##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139749_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5998639
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31538087222785	33.0	33.0	34.0	31.0	34.0
2	31.34977517400197	33.0	32.0	33.0	27.0	34.0
3	32.52315133482778	33.0	33.0	33.0	31.0	34.0
4	32.93737146042627	33.0	33.0	34.0	32.0	34.0
5	33.16531149815817	34.0	33.0	34.0	33.0	34.0
6	37.08055777318822	38.0	37.0	38.0	36.0	38.0
7	37.14533029908951	38.0	38.0	38.0	36.0	38.0
8	37.349022003157714	38.0	38.0	38.0	37.0	38.0
9	37.33738436335309	38.0	38.0	38.0	37.0	38.0
10-11	37.392578216492105	38.0	38.0	38.0	37.0	38.0
12-13	37.399270817930535	38.0	38.0	38.0	37.0	38.0
14-15	37.406697252493444	38.0	38.0	38.0	37.0	38.0
16-17	37.36667684119681	38.0	38.0	38.0	37.0	38.0
18-19	37.345795687988556	38.0	38.0	38.0	37.0	38.0
20-21	37.40551310298322	38.0	38.0	38.0	37.0	38.0
22-23	37.42167737429769	38.0	38.0	38.0	37.0	38.0
24-25	37.42418470109601	38.0	38.0	38.0	37.0	38.0
26-27	37.33077319819917	38.0	38.0	38.0	37.0	38.0
28-29	37.23546337978533	38.0	38.0	38.0	37.0	38.0
30-31	37.31950962352486	38.0	38.0	38.0	37.0	38.0
32-33	37.33964605758277	38.0	38.0	38.0	37.0	38.0
34-35	37.29780660846643	38.0	38.0	38.0	37.0	38.0
36-37	37.305406252860266	38.0	38.0	38.0	37.0	38.0
38-39	37.29954791391859	38.0	38.0	38.0	37.0	38.0
40-41	37.27409865817229	38.0	38.0	38.0	37.0	38.0
42-43	37.27852705345764	38.0	38.0	38.0	37.0	38.0
44-45	37.2193381688291	38.0	38.0	38.0	37.0	38.0
46-47	37.20113805977202	38.0	38.0	38.0	37.0	38.0
48-49	37.18488622225456	38.0	38.0	38.0	37.0	38.0
50-51	37.18834971991848	38.0	38.0	38.0	37.0	38.0
52-53	37.17541514522073	38.0	38.0	38.0	37.0	38.0
54-55	37.12862557700119	38.0	38.0	38.0	36.0	38.0
56-57	37.13752794684723	38.0	38.0	38.0	36.0	38.0
58-59	37.15222022343749	38.0	38.0	38.0	36.0	38.0
60-61	37.162748565840005	38.0	38.0	38.0	36.0	38.0
62-63	37.092441017145916	38.0	38.0	38.0	36.0	38.0
64-65	37.125428524476995	38.0	38.0	38.0	36.0	38.0
66-67	37.15067208749574	38.0	38.0	38.0	36.0	38.0
68-69	37.12643426216184	38.0	38.0	38.0	36.0	38.0
70-71	37.08151328070872	38.0	38.0	38.0	36.0	38.0
72-73	37.09870376553324	38.0	38.0	38.0	36.0	38.0
74-75	37.12212723346192	38.0	38.0	38.0	36.0	38.0
76-77	37.06414081272166	38.0	38.0	38.0	36.0	38.0
78-79	37.09634531167427	38.0	38.0	38.0	36.0	38.0
80-81	37.07755253532572	38.0	38.0	38.0	36.0	38.0
82-83	37.01232930759619	38.0	38.0	38.0	36.0	38.0
84-85	37.067647374276696	38.0	38.0	38.0	36.0	38.0
86-87	37.09647988338288	38.0	38.0	38.0	36.0	38.0
88-89	37.00615215946102	38.0	38.0	38.0	36.0	38.0
90-91	36.97502864693776	38.0	38.0	38.0	36.0	38.0
92-93	37.03815157636836	38.0	38.0	38.0	36.0	38.0
94-95	37.00994041449565	38.0	38.0	38.0	36.0	38.0
96-97	36.93228515781239	38.0	38.0	38.0	35.5	38.0
98-99	36.954977297104236	38.0	38.0	38.0	35.5	38.0
100	35.50190541924816	38.0	36.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	7.0
19	16.0
20	51.0
21	136.0
22	356.0
23	993.0
24	2340.0
25	4906.0
26	8673.0
27	13825.0
28	21492.0
29	31112.0
30	42747.0
31	57404.0
32	74943.0
33	101110.0
34	148274.0
35	269291.0
36	760059.0
37	4460902.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.39414973963261	19.818845574804552	13.007967307250862	24.77903737831198
2	15.227647623167604	20.689490192352096	40.73540063697825	23.347461547502057
3	18.4772412542245	25.54642811477737	30.06335270383832	25.912977927159808
4	12.551126231593098	17.403498430392393	38.43867427795632	31.606701060058185
5	13.311016494233522	37.54687036339327	35.877258496347395	13.264854646025814
6	30.77394724413794	37.46327692751641	18.425509363581213	13.337266464764438
7	27.169473445033226	32.24609673570629	22.580911350969462	18.003518468291023
8	24.881543963555732	36.53657104553216	21.06326118307836	17.518623807833745
9	25.669689407880686	16.443896690565975	20.841294166893523	37.04511973465981
10-11	24.343146837140893	26.61937149410058	28.903264557177057	20.134217111581478
12-13	25.01866673423755	23.94589839461918	29.086172713510518	21.949262157632756
14-15	22.046575898299597	25.542085463052537	26.289688377646996	26.121650261000873
16-17	21.985595399223058	28.83781637801508	27.04666841928644	22.129919803475424
18-19	22.071898308933076	27.98679000353247	28.562912353952285	21.37839933358217
20-21	22.599702276636002	27.167886286645803	28.51690203994434	21.715509396773854
22-23	22.309710629737317	27.218594677825124	28.390339726041557	22.081354966396
24-25	22.20466084507014	27.28375289741883	28.46307717675076	22.048509080760272
26-27	22.202835563526232	27.440301336981943	28.417610692921	21.93925240657083
28-29	22.159657426609883	27.275574833518522	28.493700840721853	22.071066899149738
30-31	22.212978029102597	27.194082618267124	28.56755064506837	22.025388707561913
32-33	21.97924068592766	27.445618681308943	28.497765980592956	22.077374652170445
34-35	22.234200014415816	27.34956006617865	28.45641813911933	21.959821780286205
36-37	22.10182211924909	27.365877302860138	28.54376217501371	21.988538402877065
38-39	21.93804033365651	27.355011348252038	28.61305314075243	22.09389517733902
40-41	22.268086448854394	27.199952940641975	28.611097973141923	21.920862637361708
42-43	22.129863996004126	27.196582141709175	28.54763428289271	22.125919579393994
44-45	22.06738168443928	27.35582245896189	28.43506740567833	22.14172845092051
46-47	22.21968596277013	27.204548634672875	28.368398414268476	22.20736698828852
48-49	22.1044239418021	27.417661462650806	28.22526891082161	22.252645684725483
50-51	21.982948048480917	27.563427046389293	28.16167413758224	22.29195076754755
52-53	22.06243878794841	27.40056746296983	28.23769179192464	22.299301957157127
54-55	22.16275651539309	27.198977744641667	28.34459122969446	22.293674510270776
56-57	22.100101051089084	27.092962619861225	28.33558426898345	22.47135206006624
58-59	21.837359737432102	27.06104461597183	28.493116764447446	22.60847888214862
60-61	22.106174515983632	27.403999730349316	28.48960809445529	22.000217659211767
62-63	21.672976791964878	27.64693356821999	28.462911655082706	22.217177984732427
64-65	21.498896146944517	27.934984737612528	28.19995767216926	22.3661614432737
66-67	21.57038397225296	28.382733995385983	27.848637624415883	22.19824440794517
68-69	21.68259004829367	28.26686627287531	27.715975981622066	22.33456769720896
70-71	21.7049281152927	27.8297704605607	27.728538685411152	22.73676273873545
72-73	21.918792354928733	27.319424220620427	27.704542605738997	23.057240818711843
74-75	21.954127480917872	27.323880766966703	27.88060647455782	22.841385277557606
76-77	22.31253027506855	27.2185621677225	27.38707837860412	23.081829178604828
78-79	22.425068528117517	27.265130954639005	27.01879627020097	23.29100424704251
80-81	22.262619315319434	27.62566810597333	27.134184030095366	22.977528548611872
82-83	22.290558189446127	27.4035370128639	27.16240226380455	23.14350253388542
84-85	22.326221536200975	27.700912419606084	27.167558944942392	22.805307099250548
86-87	22.68161380411725	27.634977341849254	27.2060633659704	22.477345488063097
88-89	22.485530577749245	27.740116435912388	27.471618245244056	22.302734741094312
90-91	22.53819861584847	27.434251225119592	27.296007582750033	22.731542576281903
92-93	22.500568398174448	27.099664843817695	27.50284000346604	22.89692675454182
94-95	21.983244948093127	27.455140365594648	27.94715443081201	22.614460255500212
96-97	22.10492349795609	27.39662233673526	28.106531909034537	22.391922256274107
98-99	23.262962315148194	29.220154470820287	28.444896203573222	19.071987010458297
100	22.186285082596907	30.30443831959592	22.20688420960404	25.30239238820313
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	778.0
1	706.5
2	973.5
3	1513.5
4	1890.5
5	2435.5
6	3102.5
7	3989.0
8	5193.0
9	6065.0
10	6103.0
11	6135.0
12	6833.5
13	8115.5
14	10337.5
15	12685.5
16	14363.0
17	15240.0
18	14924.0
19	13949.0
20	13056.0
21	12868.0
22	13954.5
23	16764.0
24	21612.5
25	28291.0
26	36975.0
27	47481.0
28	59261.5
29	72394.0
30	85837.5
31	99566.5
32	114308.0
33	128516.5
34	142393.0
35	155737.0
36	169640.0
37	182580.0
38	194344.5
39	205665.0
40	216219.0
41	225986.5
42	232995.0
43	239406.5
44	249726.5
45	262080.0
46	262956.5
47	258307.5
48	255033.0
49	249522.5
50	244463.5
51	236710.5
52	226763.0
53	214753.5
54	200517.5
55	185843.5
56	169169.0
57	151816.0
58	134451.5
59	115239.0
60	95646.5
61	77050.0
62	60766.5
63	47034.5
64	35124.5
65	25782.5
66	19066.0
67	14513.5
68	11027.0
69	8110.5
70	5871.0
71	4264.5
72	3021.0
73	2022.0
74	1369.0
75	917.0
76	593.0
77	353.0
78	218.5
79	131.5
80	88.5
81	60.5
82	44.0
83	34.5
84	26.0
85	17.5
86	11.5
87	9.5
88	7.5
89	6.0
90	5.5
91	4.0
92	2.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	6.668179231989122E-5
3	0.0
4	1.0002268847983685E-4
5	0.0029840102063151326
6	4.33431650079293E-4
7	1.0002268847983685E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.001050766183675597
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	1.703851377133616E-5
44-45	1.7138486339169617E-5
46-47	3.450691941374814E-5
48-49	1.1290094817690154E-4
50-51	5.155844578026455E-4
52-53	2.1888760095424488E-4
54-55	3.2411844163853263E-4
56-57	4.8202802335644873E-4
58-59	3.595029879959322E-4
60-61	7.049686099508257E-5
62-63	3.7247795839509887E-4
64-65	1.2496602486199065E-4
66-67	1.5287107159563768E-4
68-69	5.257856620244325E-4
70-71	1.3712069217062785E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0010657680579730891
90-91	0.0
92-93	1.9874058093859214E-5
94-95	3.0060725671929868E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	82.0
20-21	185.0
22-23	289.0
24-25	373.0
26-27	1313.0
28-29	4174.0
30-31	9744.0
32-33	12814.0
34-35	18437.0
36-37	28212.0
38-39	16458.0
40-41	29879.0
42-43	32623.0
44-45	38128.0
46-47	39276.0
48-49	36444.0
50-51	18769.0
52-53	2957.0
54-55	2751.0
56-57	2694.0
58-59	19692.0
60-61	36644.0
62-63	35850.0
64-65	39743.0
66-67	43836.0
68-69	45265.0
70-71	51044.0
72-73	38364.0
74-75	37179.0
76-77	38920.0
78-79	40236.0
80-81	39805.0
82-83	37319.0
84-85	36901.0
86-87	38543.0
88-89	40443.0
90-91	41537.0
92-93	40645.0
94-95	50027.0
96-97	198732.0
98-99	460868.0
100-101	4291444.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.97632842097815
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.17238399089725	80.21011596906706
2	6.512521646847236	11.458954865035237
3	1.4088940399546475	3.718479743082263
4	0.4604261393983187	1.6202640501323824
5	0.18444427218475798	0.8113364932547276
6	0.09142687859438549	0.48260406586347326
7	0.05071899717629601	0.31234498069351363
8	0.032024198122882576	0.22538970971817512
9	0.022733367364269168	0.17999983740183217
>10	0.06296665498018353	0.8400486604807316
>50	0.0011041235501013057	0.06602475170891199
>100	3.2909374867944816E-4	0.05854880095680031
>500	2.6597180965030937E-5	0.015888072604755388
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6670448079972806E-5
2	0.0	3.334089615994561E-5	0.0	1.6670448079972806E-5	1.6670448079972806E-5
3	0.0	3.334089615994561E-5	0.0	3.334089615994561E-5	1.6670448079972806E-5
4	0.0	1.1669313655980965E-4	0.0	8.335224039986403E-5	1.6670448079972806E-5
5	0.0	1.3336358463978244E-4	0.0	8.335224039986403E-5	1.6670448079972806E-5
6	1.3336358463978244E-4	1.3336358463978244E-4	0.0	8.335224039986403E-5	3.334089615994561E-5
7	1.3336358463978244E-4	1.3336358463978244E-4	0.0	8.335224039986403E-5	3.334089615994561E-5
8	1.3336358463978244E-4	1.3336358463978244E-4	0.0	8.335224039986403E-5	3.334089615994561E-5
9	1.3336358463978244E-4	2.333862731196193E-4	0.0	1.0002268847983685E-4	5.0011344239918424E-5
10-11	1.4169880867976887E-4	2.333862731196193E-4	0.0	1.0835791251982325E-4	5.0011344239918424E-5
12-13	1.5003403271975527E-4	2.333862731196193E-4	0.0	1.1669313655980965E-4	5.0011344239918424E-5
14-15	1.5003403271975527E-4	3.0840328947949697E-4	0.0	1.2502836059979605E-4	6.668179231989122E-5
16-17	2.000453769596737E-4	3.1673851351948334E-4	0.0	1.5003403271975527E-4	6.668179231989122E-5
18-19	2.083806009996601E-4	3.3340896159945614E-4	0.0	2.000453769596737E-4	1.0835791251982325E-4
20-21	2.167158250396465E-4	3.5007940967942894E-4	0.0	2.8339761735953774E-4	1.1669313655980965E-4
22-23	2.333862731196193E-4	3.91755529879361E-4	0.0	3.7508508179938816E-4	1.3336358463978244E-4
24-25	2.333862731196193E-4	4.667725462392386E-4	0.0	4.584373221992522E-4	1.5003403271975527E-4
26-27	2.333862731196193E-4	5.001134423991842E-4	0.0	5.834656827990482E-4	1.5836925675974167E-4
28-29	2.333862731196193E-4	5.001134423991842E-4	0.0	0.0012002722617580422	1.7503970483971447E-4
30-31	2.333862731196193E-4	5.16783890479157E-4	0.0	0.0028839875178352957	1.8337492887970087E-4
32-33	2.500567211995921E-4	5.334543385591298E-4	0.0	0.005584600106790891	2.167158250396465E-4
34-35	2.917328413995241E-4	6.00136130879021E-4	0.0	0.009068723755505208	2.333862731196193E-4
36-37	3.0006806543951054E-4	6.751531472388988E-4	0.0	0.018620890505329626	2.500567211995921E-4
38-39	3.0006806543951054E-4	7.001588193588579E-4	0.0	0.15905274513102055	2.500567211995921E-4
40-41	3.0006806543951054E-4	7.334997155188035E-4	0.0	0.3038355867055844	2.500567211995921E-4
42-43	3.0006806543951054E-4	7.334997155188035E-4	0.0	0.32388180052175164	2.500567211995921E-4
44-45	3.1673851351948334E-4	8.168519559186675E-4	0.0	0.35121300014886714	2.917328413995241E-4
46-47	3.3340896159945614E-4	9.002041963185316E-4	0.0	0.38158655655057755	3.1673851351948334E-4
48-49	3.3340896159945614E-4	9.168746443985044E-4	0.0	0.410118028439451	3.4174418563944257E-4
50-51	3.584146337194153E-4	0.0010085621088383547	0.0	0.4376242677714062	3.5007940967942894E-4
52-53	3.6674985775940174E-4	0.001033567780958314	0.0	0.8237951975439762	3.584146337194153E-4
54-55	3.6674985775940174E-4	0.0010419030049983004	0.0	1.3455802224471252	3.7508508179938816E-4
56-57	3.6674985775940174E-4	0.0010669086771182596	0.0	1.9211941242005062	3.91755529879361E-4
58-59	3.6674985775940174E-4	0.0011002495732782053	0.0	2.508835754243588	4.000907539193474E-4
60-61	4.0842597795933376E-4	0.0011419256934781373	0.0	2.6639209327315747	4.000907539193474E-4
62-63	4.834429943192114E-4	0.0011502609175181237	0.0	2.6701890212096444	4.000907539193474E-4
64-65	4.834429943192114E-4	0.001283624502157906	0.0	2.6758069622125955	4.000907539193474E-4
66-67	4.834429943192114E-4	0.001283624502157906	0.0	2.6799078924402684	4.000907539193474E-4
68-69	4.834429943192114E-4	0.0013169653983178518	0.0	2.6839421408756223	4.0842597795933376E-4
70-71	4.917782183591978E-4	0.001341971070437811	1.6670448079972806E-5	2.687267895267577	4.167612019993202E-4
72-73	5.16783890479157E-4	0.0013586415185177837	1.6670448079972806E-5	2.690218564577732	4.834429943192114E-4
74-75	5.16783890479157E-4	0.0014169880867976885	1.6670448079972806E-5	2.6924690750685283	5.001134423991842E-4
76-77	5.16783890479157E-4	0.0014920051031575663	1.6670448079972806E-5	2.6946195628708445	5.001134423991842E-4
78-79	5.16783890479157E-4	0.0015003403271975527	1.6670448079972806E-5	2.6959948748374423	5.001134423991842E-4
80-81	5.16783890479157E-4	0.0015003403271975527	1.6670448079972806E-5	2.6965283291760014	5.084486664391707E-4
82-83	5.16783890479157E-4	0.0015170107752775254	1.6670448079972806E-5	2.696853402913561	5.251191145191434E-4
84-85	5.251191145191434E-4	0.001525345999317512	1.6670448079972806E-5	2.6970701187386004	5.334543385591298E-4
86-87	5.334543385591298E-4	0.001550351671437471	1.6670448079972806E-5	2.6971951470992	5.417895625991162E-4
88	5.667952347190755E-4	0.0016170334637573623	1.6670448079972806E-5	2.6971951470992	5.501247866391026E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6505	0.0	45.00088	1
GTATCAA	10960	0.0	35.143433	1
TCAACGC	12115	0.0	30.957283	4
ATCAACG	12370	0.0	30.212362	3
CAACGCA	12505	0.0	30.027004	5
AACGCAG	12925	0.0	29.083876	6
TATCAAC	13625	0.0	27.558731	2
ACGCAGA	15385	0.0	24.40487	7
GTACATG	17085	0.0	23.652353	1
CGCAGAG	15915	0.0	23.59214	8
TACATGG	16995	0.0	23.052368	2
ACATGGG	16980	0.0	22.787563	3
TATCTCG	8780	0.0	22.212837	94
ATCTCGT	7850	0.0	21.95372	94
CATGGGG	12460	0.0	20.066748	4
GCAGAGT	19305	0.0	19.494911	9
GAGTACT	10375	0.0	19.070698	12-13
AGAGTAC	16625	0.0	17.845274	10-11
CAGAGTA	18685	0.0	17.656454	10-11
GAGTACA	11925	0.0	17.238726	1
>>END_MODULE