FastQCFastQC Report
Fri 27 May 2016
SRR2139748_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139748_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6538841
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65350.044.3966451
GTATCAA110600.034.043151
TCAACGC128300.030.366794
ATCAACG129500.030.0517083
CAACGCA132900.029.315725
AACGCAG137950.028.2416826
CTTATAC66000.027.697731
TATCAAC142250.027.603512
ACGCAGA161450.024.1577767
CGCAGAG168950.023.0595478
TATACAC96850.022.433853
TACATGG168350.022.0541612
GTACATG166450.021.7554651
ACATGGG169400.021.3508513
TTATACA95300.020.6012552
GCAGAGT207150.018.9124979
TATGCCG52450.018.73339842-43
CATGGGG121650.018.4700984
GAGTACT116250.018.38910512-13
GTGGTAT37100.018.2277011
Produced by FastQC (version 0.11.5)