##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139748_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6538841
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	20.88950213042342	18.0	18.0	18.0	18.0	32.0
2	30.494060950556833	32.0	28.0	32.0	27.0	33.0
3	31.27701820551991	33.0	31.0	33.0	27.0	33.0
4	31.737284023269567	33.0	32.0	33.0	30.0	33.0
5	32.435698314120195	33.0	33.0	33.0	32.0	34.0
6	36.163690476645634	38.0	36.0	38.0	33.0	38.0
7	36.65767526691657	38.0	37.0	38.0	34.0	38.0
8	36.5682014595553	38.0	37.0	38.0	34.0	38.0
9	37.07339312884348	38.0	38.0	38.0	36.0	38.0
10-11	37.184144789573566	38.0	38.0	38.0	36.0	38.0
12-13	37.255634752397256	38.0	38.0	38.0	36.0	38.0
14-15	37.256577962363664	38.0	38.0	38.0	36.5	38.0
16-17	37.213847683404445	38.0	38.0	38.0	36.0	38.0
18-19	37.24556500150409	38.0	38.0	38.0	36.5	38.0
20-21	37.32631579576537	38.0	38.0	38.0	37.0	38.0
22-23	37.36311396473801	38.0	38.0	38.0	37.0	38.0
24-25	37.37112936337489	38.0	38.0	38.0	37.0	38.0
26-27	37.288894570655316	38.0	38.0	38.0	37.0	38.0
28-29	37.200150035655554	38.0	38.0	38.0	36.5	38.0
30-31	37.283248535370525	38.0	38.0	38.0	37.0	38.0
32-33	37.304695069546824	38.0	38.0	38.0	37.0	38.0
34-35	37.26043858715331	38.0	38.0	38.0	37.0	38.0
36-37	37.267992325303666	38.0	38.0	38.0	37.0	38.0
38-39	37.25654907761101	38.0	38.0	38.0	37.0	38.0
40-41	37.2318868477456	38.0	38.0	38.0	37.0	38.0
42-43	37.23746883168496	38.0	38.0	38.0	37.0	38.0
44-45	37.17796321725028	38.0	38.0	38.0	36.5	38.0
46-47	37.155800991375855	38.0	38.0	38.0	36.5	38.0
48-49	37.134789711533216	38.0	38.0	38.0	36.0	38.0
50-51	37.12838034837904	38.0	38.0	38.0	36.0	38.0
52-53	37.10604702634873	38.0	38.0	38.0	36.0	38.0
54-55	37.05011567514742	38.0	38.0	38.0	36.0	38.0
56-57	37.052289960862154	38.0	38.0	38.0	36.0	38.0
58-59	37.062888559895605	38.0	38.0	38.0	36.0	38.0
60-61	37.067583659646964	38.0	38.0	38.0	36.0	38.0
62-63	36.991337438281846	38.0	38.0	38.0	36.0	38.0
64-65	37.02146347432278	38.0	38.0	38.0	36.0	38.0
66-67	37.03664921063789	38.0	38.0	38.0	36.0	38.0
68-69	37.005462897181516	38.0	38.0	38.0	36.0	38.0
70-71	36.9556746709732	38.0	38.0	38.0	36.0	38.0
72-73	36.95845535855942	38.0	38.0	38.0	36.0	38.0
74-75	36.97703079527919	38.0	38.0	38.0	36.0	38.0
76-77	36.91570539081728	38.0	38.0	38.0	35.5	38.0
78-79	36.942364446501195	38.0	38.0	38.0	36.0	38.0
80-81	36.91805493505398	38.0	38.0	38.0	36.0	38.0
82-83	36.86188853363862	38.0	38.0	38.0	35.0	38.0
84-85	36.89345389650053	38.0	38.0	38.0	35.0	38.0
86-87	36.913951844731955	38.0	38.0	38.0	35.0	38.0
88-89	36.823934743850415	38.0	38.0	38.0	35.0	38.0
90-91	36.7875942596805	38.0	38.0	38.0	35.0	38.0
92-93	36.8371122981691	38.0	38.0	38.0	35.0	38.0
94-95	36.81840563353643	38.0	38.0	38.0	35.0	38.0
96-97	36.7628990345853	38.0	38.0	38.0	35.0	38.0
98-99	36.775607294591325	38.0	38.0	38.0	35.0	38.0
100	35.164619684083675	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	6.0
18	27.0
19	36.0
20	86.0
21	220.0
22	622.0
23	1799.0
24	3690.0
25	7166.0
26	11886.0
27	19012.0
28	28601.0
29	40642.0
30	56234.0
31	75597.0
32	100812.0
33	138934.0
34	210789.0
35	394707.0
36	1217481.0
37	4230494.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.541618919927856	16.87959685821998	17.766543031096795	24.812241190755365
2	14.764833706768524	21.05307347280657	40.66622815878227	23.515864661642635
3	18.29018322971915	25.139638660735137	30.76863315685456	25.80154495269116
4	12.181435989744351	17.472424369172796	38.62574908221419	31.720390558868665
5	13.156413661809587	37.352255118651655	35.976264774488136	13.51506644505062
6	30.413045536204624	37.75157256990903	18.509932756970336	13.32544913691601
7	26.895726733098453	32.20790911168009	22.76404462077207	18.132319534449394
8	24.76201822310712	36.52364386899758	21.183081221886262	17.531256686009034
9	25.52606799890072	16.38252100028124	20.88204928059881	37.20936172021923
10-11	24.242193685394707	26.604110728491488	29.068553586178346	20.085141999935463
12-13	24.909582600341558	23.95626380883095	29.14371216550456	21.990441425322928
14-15	21.889437287127794	25.52342991670848	26.40250772269887	26.184625073464858
16-17	21.892144188855486	28.84667175727319	27.11518753858673	22.145996515284587
18-19	21.93891853311619	27.976624603656823	28.6542982158459	21.430158647381088
20-21	22.499738481565856	27.145460529374482	28.657832339461326	21.696968649598332
22-23	22.201315343935736	27.25593169583689	28.50203211186388	22.0407208483635
24-25	22.12630097446138	27.324126790101705	28.649251717910317	21.900320517526602
26-27	22.182012413323253	27.48458812359726	28.56475404858594	21.76864541449355
28-29	22.1221183696711	27.37637844045418	28.5576469128358	21.943856277038922
30-31	22.125646470829388	27.457247663317396	28.673189403108207	21.743916462745013
32-33	22.059406870058286	27.528919755444107	28.584647672445556	21.827025702052048
34-35	22.3276997028685	27.3649764237961	28.58042843017164	21.726895443163762
36-37	22.001328335058794	27.519440704924524	28.563514837496434	21.915716122520244
38-39	21.852017259002185	27.366054299781258	28.829713017331528	21.952215423885036
40-41	22.12473190812434	27.241124287420803	28.731865769968586	21.902278034486265
42-43	22.10894843853517	27.268036623564473	28.64206965637478	21.980945281525578
44-45	22.024141617858348	27.505617142992474	28.51779259596941	21.95244864317977
46-47	22.20502532507956	27.301797071351874	28.28387593177833	22.209301671790232
48-49	22.057699683505863	27.395959765997745	28.31583959578531	22.230500954711083
50-51	22.000810892909207	27.505845132926453	28.373064305924743	22.12027966823959
52-53	22.13358600433134	27.458025868894488	28.421786859393606	21.98660126738057
54-55	22.10208297113651	27.455876828359433	28.23514202775917	22.20689817274489
56-57	22.188204444129266	27.65361696335087	28.090604123949152	22.06757446857071
58-59	21.97253845758993	27.723371026836226	28.343843595879157	21.960246919694683
60-61	22.152241091471353	27.92239692343711	28.05827564612927	21.867086338962267
62-63	21.968464324716408	28.112898544216787	28.026825302611357	21.89181182845545
64-65	21.9693646516355	28.03358842880332	28.01279573513366	21.984251184427517
66-67	21.974770617883063	28.03632044105725	27.949653661067675	22.039255279992016
68-69	22.022897629277804	27.974896858366407	27.97768767977046	22.024517832585325
70-71	22.09080426608796	27.69718032117202	28.075151519920638	22.136863892819377
72-73	22.12308927230513	27.565661709491714	28.066891652784854	22.24435736541831
74-75	22.038310487264525	27.692259564478572	28.02062772124856	22.24880222700834
76-77	22.19005592916514	27.588764475488013	27.938590774006105	22.28258882134074
78-79	22.169611906453223	27.584301868119493	27.91170607036186	22.334380155065425
80-81	22.16564461560243	27.65420307312122	27.874951900651045	22.305200410625307
82-83	22.257486722315175	27.617432439404038	27.90786461138123	22.217216226899563
84-85	22.268629266922044	27.637033649938974	27.91332422057907	22.18101286255991
86-87	22.182693011186934	27.62203675597109	27.95941360091147	22.235856631930503
88-89	22.244188846106493	27.591705402821297	27.933142547953004	22.230963203119206
90-91	22.333719945506093	27.673128612982804	27.859530910563716	22.133620530947383
92-93	22.2943189177053	27.649569794908047	27.934004293701026	22.122106993685627
94-95	22.2201206678827	27.749450446658248	27.877246153126606	22.153182732332443
96-97	22.344041341980798	27.813687813011263	27.949490663506648	21.892780181501287
98-99	23.33938717196567	29.753629135591954	28.199743568469138	18.707240123973243
100	22.253875603577605	30.61522955041747	22.227188962560607	24.903705883444317
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	851.0
1	889.0
2	1284.0
3	1795.5
4	2219.0
5	2885.0
6	3641.0
7	5042.0
8	6459.0
9	7035.5
10	7019.5
11	6996.5
12	7756.0
13	9329.5
14	12177.0
15	15249.0
16	17150.5
17	17804.5
18	17335.5
19	16289.0
20	15414.0
21	15413.0
22	17132.0
23	21133.0
24	27342.0
25	35799.5
26	46554.5
27	58864.5
28	72128.5
29	87231.5
30	102373.5
31	117220.0
32	132984.5
33	148298.0
34	162731.5
35	176853.5
36	191713.5
37	204994.5
38	216798.5
39	227736.0
40	238067.0
41	248014.5
42	256851.0
43	270890.5
44	279581.0
45	280260.5
46	278961.5
47	274156.0
48	270245.5
49	265883.0
50	260074.5
51	251557.5
52	241419.5
53	228473.0
54	213532.0
55	198758.0
56	182817.0
57	165481.5
58	146738.0
59	126693.0
60	106341.5
61	86597.5
62	68505.0
63	52788.0
64	39245.0
65	28732.5
66	21323.0
67	16441.5
68	12329.5
69	8844.0
70	6424.0
71	4589.5
72	3232.5
73	2235.5
74	1530.0
75	1005.5
76	657.0
77	394.0
78	240.0
79	162.0
80	99.0
81	61.5
82	44.0
83	35.5
84	23.5
85	13.0
86	10.5
87	6.5
88	2.5
89	1.5
90	2.0
91	2.0
92	1.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	9.175938059971178E-5
5	0.0031351121704901526
6	3.364510621989432E-4
7	4.587969029985589E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0011934489749688612
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	1.5647348814924567E-5
44-45	1.573594455597295E-5
46-47	3.959578719830188E-5
48-49	1.1158741223849292E-4
50-51	3.7689698467176003E-4
52-53	1.612379332553963E-4
54-55	2.9988793917170306E-4
56-57	4.238618921015537E-4
58-59	3.279919300865521E-4
60-61	8.249572672135583E-6
62-63	3.984087884994655E-4
64-65	4.1769154248140186E-5
66-67	1.1781080594372347E-4
68-69	5.004791451612616E-4
70-71	1.0263973137813768E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	8.402962226858059E-4
90-91	0.0
92-93	2.783297210774366E-5
94-95	6.547865316397062E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	73.0
20-21	183.0
22-23	336.0
24-25	411.0
26-27	1365.0
28-29	4290.0
30-31	9958.0
32-33	13242.0
34-35	18907.0
36-37	29130.0
38-39	31384.0
40-41	30725.0
42-43	34086.0
44-45	40492.0
46-47	41271.0
48-49	38800.0
50-51	34299.0
52-53	32442.0
54-55	34893.0
56-57	35620.0
58-59	36807.0
60-61	36945.0
62-63	38126.0
64-65	41701.0
66-67	46671.0
68-69	47675.0
70-71	55496.0
72-73	40225.0
74-75	38190.0
76-77	40141.0
78-79	41645.0
80-81	41391.0
82-83	38723.0
84-85	38166.0
86-87	40352.0
88-89	42079.0
90-91	42819.0
92-93	42531.0
94-95	53572.0
96-97	200446.0
98-99	467955.0
100-101	4635278.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.86450803174989
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.18437972441066	80.11870664665614
2	6.434762792001446	11.30774534040433
3	1.4296698159385672	3.7685170507585393
4	0.47348342666441307	1.6640955338022314
5	0.20068701376001769	0.8816632866192489
6	0.09638063042228985	0.5081062005506642
7	0.05745636636568777	0.35338627548091966
8	0.033879228675763366	0.23814254080728814
9	0.023721106390526738	0.18758190086751877
>10	0.06432177163558325	0.8399431677704003
>50	9.811864896871595E-4	0.05656616035540602
>100	2.273546435177403E-4	0.044287644189957814
>500	4.9582601831408494E-5	0.03125825173735547
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.058646019990393E-5	0.0	0.0	0.0
3	0.0	3.058646019990393E-5	0.0	0.0	0.0
4	0.0	3.058646019990393E-5	0.0	0.0	0.0
5	0.0	4.587969029985589E-5	0.0	0.0	0.0
6	6.117292039980786E-5	4.587969029985589E-5	0.0	0.0	0.0
7	6.117292039980786E-5	4.587969029985589E-5	0.0	0.0	0.0
8	7.646615049975981E-5	6.117292039980786E-5	0.0	1.5293230099951965E-5	0.0
9	7.646615049975981E-5	1.0705261069966375E-4	0.0	4.587969029985589E-5	1.5293230099951965E-5
10-11	9.175938059971178E-5	1.0705261069966375E-4	0.0	8.41127655497358E-5	1.5293230099951965E-5
12-13	1.0705261069966375E-4	1.0705261069966375E-4	0.0	9.175938059971178E-5	1.5293230099951965E-5
14-15	1.299924558495917E-4	1.9116537624939956E-4	0.0	9.175938059971178E-5	1.5293230099951965E-5
16-17	1.8351876119942356E-4	1.9881199129937553E-4	0.0	9.175938059971178E-5	1.5293230099951965E-5
18-19	1.8351876119942356E-4	2.2939845149927944E-4	0.0	9.175938059971178E-5	1.5293230099951965E-5
20-21	1.8351876119942356E-4	2.599849116991834E-4	0.0	9.940599564968777E-5	1.5293230099951965E-5
22-23	1.9881199129937553E-4	2.829247568491113E-4	0.0	1.299924558495917E-4	2.2939845149927945E-5
24-25	1.9881199129937553E-4	3.440976772489192E-4	0.0	1.3763907089956769E-4	3.058646019990393E-5
26-27	1.9881199129937553E-4	3.593909073488712E-4	0.0	1.6057891604949562E-4	3.058646019990393E-5
28-29	1.9881199129937553E-4	3.670375223988471E-4	0.0	6.423156641979825E-4	3.8233075249879907E-5
30-31	1.9881199129937553E-4	4.3585705784863094E-4	0.0	0.0019193003775439714	4.587969029985589E-5
32-33	2.064586063493515E-4	4.5115028794858293E-4	0.0	0.004412096883836141	4.587969029985589E-5
34-35	2.2939845149927947E-4	5.352630534983187E-4	0.0	0.007638968434926006	4.587969029985589E-5
36-37	2.4469168159923143E-4	6.652555093479104E-4	0.0	0.013091004965558881	4.587969029985589E-5
38-39	2.599849116991834E-4	6.881953544978384E-4	0.0	0.026097897165568026	4.587969029985589E-5
40-41	2.599849116991834E-4	7.340750447976942E-4	0.0	0.03938771412242628	4.587969029985589E-5
42-43	2.599849116991834E-4	7.799547350975501E-4	0.0	0.04767664483660025	5.3526305349831875E-5
44-45	2.599849116991834E-4	9.787667263969257E-4	0.0	0.05808368791961756	6.117292039980786E-5
46-47	2.599849116991834E-4	0.0011087591822465173	0.0	0.06878130237453396	6.117292039980786E-5
48-49	2.599849116991834E-4	0.0011316990273964453	0.0	0.07967008220569977	6.117292039980786E-5
50-51	2.6763152674915937E-4	0.0012922779434459408	0.0	0.09023770420476657	6.117292039980786E-5
52-53	2.7527814179913537E-4	0.0013305110186958209	0.0	0.11568563909108665	6.117292039980786E-5
54-55	2.7527814179913537E-4	0.001368744093945701	0.0	0.14569860316224237	6.117292039980786E-5
56-57	2.7527814179913537E-4	0.0014069771691955807	0.0	0.1887031662033073	6.117292039980786E-5
58-59	2.7527814179913537E-4	0.0014222703992955326	0.0	0.2223865054984515	6.117292039980786E-5
60-61	2.829247568491113E-4	0.0014681500895953885	0.0	0.2405625094722444	6.881953544978384E-5
62-63	3.2115783209899125E-4	0.0014757967046453645	0.0	0.25420407072140155	7.646615049975981E-5
64-65	3.2115783209899125E-4	0.0017051951561446442	0.0	0.25892203220723675	7.646615049975981E-5
66-67	3.2115783209899125E-4	0.0017587214614944759	0.0	0.26355588092752213	7.646615049975981E-5
68-69	3.2115783209899125E-4	0.0018275409969442597	0.0	0.268075030422058	7.646615049975981E-5
70-71	3.517442922988952E-4	0.0018734206872441156	0.0	0.27184481164169616	7.646615049975981E-5
72-73	3.670375223988471E-4	0.0018963605323940435	0.0	0.27513285611318583	7.646615049975981E-5
74-75	3.670375223988471E-4	0.0019040071474440195	0.0	0.277312141402429	7.646615049975981E-5
76-77	3.746841374488231E-4	0.0019116537624939956	0.0	0.27944554700137225	7.646615049975981E-5
78-79	3.823307524987991E-4	0.0019116537624939956	0.0	0.2810513361618672	7.646615049975981E-5
80-81	3.823307524987991E-4	0.0019116537624939956	0.0	0.28145660675951595	7.646615049975981E-5
82-83	3.823307524987991E-4	0.0019193003775439714	0.0	0.2817701179765649	7.646615049975981E-5
84-85	3.823307524987991E-4	0.0019269469925939475	0.0	0.2819001104324146	7.646615049975981E-5
86-87	3.823307524987991E-4	0.0019422402226938994	0.0	0.2819689299678643	7.646615049975981E-5
88	3.823307524987991E-4	0.001972826682893803	0.0	0.2819765765829143	7.646615049975981E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6535	0.0	44.396645	1
GTATCAA	11060	0.0	34.04315	1
TCAACGC	12830	0.0	30.36679	4
ATCAACG	12950	0.0	30.051708	3
CAACGCA	13290	0.0	29.31572	5
AACGCAG	13795	0.0	28.241682	6
CTTATAC	6600	0.0	27.69773	1
TATCAAC	14225	0.0	27.60351	2
ACGCAGA	16145	0.0	24.157776	7
CGCAGAG	16895	0.0	23.059547	8
TATACAC	9685	0.0	22.43385	3
TACATGG	16835	0.0	22.054161	2
GTACATG	16645	0.0	21.755465	1
ACATGGG	16940	0.0	21.350851	3
TTATACA	9530	0.0	20.601255	2
GCAGAGT	20715	0.0	18.912497	9
TATGCCG	5245	0.0	18.733398	42-43
CATGGGG	12165	0.0	18.470098	4
GAGTACT	11625	0.0	18.389105	12-13
GTGGTAT	3710	0.0	18.227701	1
>>END_MODULE