FastQCFastQC Report
Fri 27 May 2016
SRR2139747_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139747_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1159265
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11800.036.95431
GTATCAA21350.033.5118641
TCAACGC22400.032.8859944
ATCAACG22600.032.4076423
CAACGCA23050.031.9586225
AACGCAG23950.030.755026
TGGACCG1401.5643309E-930.2399965
TATCAAC25250.028.8387742
ACGCAGA28700.025.5163047
CGCAGAG29300.025.1382588
CTTACGC850.001254518224.9035243
ACCGACC1353.2731376E-521.9491588
ACACCGT2156.2918843E-921.6584076
GCAGAGT34600.021.0429139
GGACCGT1656.5958575E-620.524776
GTACATG24250.018.6802121
GACCGTG2052.060884E-618.5841277
TACATGG24150.018.5822582
GAGTACT21350.018.24078412-13
TATACTG6500.018.2370435
Produced by FastQC (version 0.11.5)