##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139747_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1159265
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	20.65388241687621	18.0	18.0	18.0	18.0	32.0
2	30.33571789021492	32.0	28.0	32.0	27.0	33.0
3	31.224932176853436	32.0	31.0	33.0	27.0	33.0
4	31.70485436893204	33.0	32.0	33.0	30.0	33.0
5	32.41431683006043	33.0	33.0	33.0	32.0	34.0
6	36.173326202378234	38.0	36.0	38.0	33.0	38.0
7	36.69062121257866	38.0	37.0	38.0	34.0	38.0
8	36.61819687474391	38.0	37.0	38.0	34.0	38.0
9	37.10528653931586	38.0	38.0	38.0	36.0	38.0
10-11	37.21699913307138	38.0	38.0	38.0	36.0	38.0
12-13	37.29131777462444	38.0	38.0	38.0	37.0	38.0
14-15	37.2927216814102	38.0	38.0	38.0	36.5	38.0
16-17	37.24781564180752	38.0	38.0	38.0	36.5	38.0
18-19	37.27767680383691	38.0	38.0	38.0	37.0	38.0
20-21	37.3583606874751	38.0	38.0	38.0	37.0	38.0
22-23	37.392277324510715	38.0	38.0	38.0	37.0	38.0
24-25	37.399771225077586	38.0	38.0	38.0	37.0	38.0
26-27	37.31487581512914	38.0	38.0	38.0	37.0	38.0
28-29	37.228285009792046	38.0	38.0	38.0	37.0	38.0
30-31	37.30997578185112	38.0	38.0	38.0	37.0	38.0
32-33	37.32918911448431	38.0	38.0	38.0	37.0	38.0
34-35	37.2864234984837	38.0	38.0	38.0	37.0	38.0
36-37	37.29357323224986	38.0	38.0	38.0	37.0	38.0
38-39	37.287643588330766	38.0	38.0	38.0	37.0	38.0
40-41	37.25528277972428	38.0	38.0	38.0	37.0	38.0
42-43	37.26203123223061	38.0	38.0	38.0	37.0	38.0
44-45	37.20389166944407	38.0	38.0	38.0	37.0	38.0
46-47	37.1849721802221	38.0	38.0	38.0	37.0	38.0
48-49	37.171304312304095	38.0	38.0	38.0	37.0	38.0
50-51	37.1619675503095	38.0	38.0	38.0	36.0	38.0
52-53	37.140962696350456	38.0	38.0	38.0	36.0	38.0
54-55	37.08621638772787	38.0	38.0	38.0	36.0	38.0
56-57	37.08534029035273	38.0	38.0	38.0	36.0	38.0
58-59	37.09911116318576	38.0	38.0	38.0	36.0	38.0
60-61	37.10047579248831	38.0	38.0	38.0	36.0	38.0
62-63	37.02721427545706	38.0	38.0	38.0	36.0	38.0
64-65	37.054928204219394	38.0	38.0	38.0	36.0	38.0
66-67	37.0749136144089	38.0	38.0	38.0	36.0	38.0
68-69	37.0422881255888	38.0	38.0	38.0	36.0	38.0
70-71	36.9913118564282	38.0	38.0	38.0	36.0	38.0
72-73	36.99248830016603	38.0	38.0	38.0	36.0	38.0
74-75	37.016712938246776	38.0	38.0	38.0	36.0	38.0
76-77	36.95078758972513	38.0	38.0	38.0	36.0	38.0
78-79	36.977193809697795	38.0	38.0	38.0	36.0	38.0
80-81	36.95231944041764	38.0	38.0	38.0	36.0	38.0
82-83	36.894538206770164	38.0	38.0	38.0	36.0	38.0
84-85	36.92729998942974	38.0	38.0	38.0	36.0	38.0
86-87	36.95173170539398	38.0	38.0	38.0	36.0	38.0
88-89	36.86000861372271	38.0	38.0	38.0	35.5	38.0
90-91	36.816991035324975	38.0	38.0	38.0	35.0	38.0
92-93	36.87518913973878	38.0	38.0	38.0	35.0	38.0
94-95	36.85741624746057	38.0	38.0	38.0	35.0	38.0
96-97	36.79894791545155	38.0	38.0	38.0	35.0	38.0
98-99	36.81279930531296	38.0	38.0	38.0	35.0	38.0
100	35.217595003297845	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	6.0
19	7.0
20	11.0
21	37.0
22	112.0
23	277.0
24	579.0
25	1124.0
26	2024.0
27	3136.0
28	4797.0
29	6820.0
30	9468.0
31	12752.0
32	17232.0
33	23621.0
34	36164.0
35	69244.0
36	225294.0
37	746559.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.343752291322524	16.645374439839035	18.141581088016974	24.86929218082147
2	14.828619858272269	20.973979202339414	40.54543180377222	23.651969135616103
3	18.42891832324792	24.8766244128823	30.673055772407515	26.021401491462264
4	12.32859814502995	17.242146741380736	38.520388798410025	31.908866315179285
5	13.305481127729651	37.1478432800156	35.88095036809425	13.665725224160507
6	30.786476533716794	37.57132789135129	18.317649464011062	13.324546110920856
7	27.070255722375812	32.16701962019038	22.694120843810516	18.068603813623287
8	24.733689018472912	36.61078355682264	21.0601544944426	17.595372930261846
9	25.363139575506892	16.44835305128681	21.049113015574523	37.13939435763178
10-11	24.342967311184243	26.550702384700653	28.96162654785575	20.144703756259354
12-13	24.8728289045214	23.93072334625819	29.156189482128763	22.040258267091648
14-15	21.92415021586954	25.576809443914893	26.376971615635767	26.1220687245798
16-17	21.934587863862014	28.758782504431686	27.116060607367597	22.190569024338696
18-19	21.95710212936645	27.93593354409906	28.697924978326782	21.4090393482077
20-21	22.474500446846026	27.172853500063837	28.67099537971561	21.681650673374534
22-23	22.25374323396954	27.187958158768417	28.50447919951104	22.053819407751003
24-25	22.11881895388523	27.256217712240794	28.604452734343994	22.02051059952998
26-27	22.06272969921617	27.460583086027167	28.546659436223916	21.930027778532747
28-29	22.006816652589393	27.37429396026626	28.579055517694858	22.03983386944949
30-31	22.271104089714093	27.291249315096906	28.540279215762194	21.897367379426807
32-33	22.053947587239115	27.42159566506832	28.56219164888162	21.962265098810942
34-35	22.20854429390036	27.345006967345565	28.546439703792252	21.900009034961826
36-37	22.015695578893908	27.37642469424948	28.57852785121007	22.029351875646547
38-39	21.86818847985426	27.366022627806792	28.761803707004113	22.003985185334834
40-41	22.137645984311867	27.25501947194646	28.634044614088356	21.97328992965331
42-43	22.05703882143937	27.290711651419823	28.522929050411445	22.12932047672936
44-45	22.083430412779563	27.386190342155103	28.440708812389538	22.0896704326758
46-47	22.244353322308058	27.29512244851725	28.24109104913799	22.219433180036702
48-49	22.05100414554451	27.443824785005617	28.250662532554987	22.25450853689489
50-51	21.946398921168832	27.614829960909443	28.287669242100804	22.15110187582092
52-53	22.1537298183166	27.507267661545313	28.21787312251108	22.12112939762701
54-55	22.113977921037545	27.39887584884381	28.183137920156298	22.304008309962345
56-57	22.157317816779244	27.5491470464504	28.113103200525625	22.180431936244734
58-59	22.02642814627148	27.66289376087421	28.20908435334697	22.10159373950734
60-61	22.14630238625143	27.692446192959626	28.077485411789286	22.083766008999653
62-63	22.02675834886528	27.794826745132944	28.078920414902115	22.099494491099662
64-65	21.966034771402594	27.80471562751623	28.1336091193775	22.095640481703676
66-67	22.085888962801196	27.83404304007759	27.94162589794785	22.138442099173368
68-69	21.98271330256627	27.862934032384523	27.93928428025998	22.215068384789227
70-71	22.065287547173632	27.585393448971484	28.005457081170288	22.3438619226846
72-73	22.171505518108255	27.524288124355195	28.021903472145777	22.282302885390774
74-75	22.042294479383962	27.639682675083172	27.976239405054553	22.341783440478313
76-77	22.177926139196916	27.550475343094483	27.900631963252877	22.370966554455723
78-79	22.21614199654676	27.559640153833815	27.79293436733522	22.431283482284204
80-81	22.18714772492655	27.647227973621753	27.796073778437417	22.369550523014283
82-83	22.2291776524722	27.609391664051643	27.763489490830157	22.397941192646005
84-85	22.283279465702822	27.524616254407725	27.876852823175824	22.31525145671363
86-87	22.236334030914033	27.575770415791478	27.883325913628028	22.30456963966646
88-89	22.25766689620724	27.63052941549477	27.835725338943174	22.27607834935482
90-91	22.301952312306973	27.71022468099371	27.727548071001152	22.260274935698163
92-93	22.306447007213904	27.639571011050094	27.833428327969266	22.220553653766736
94-95	22.255617122608697	27.739578844535167	27.78100284042038	22.22380119243575
96-97	22.39178237729431	27.81440071843978	27.834981024606915	21.958835879659
98-99	23.38862130751401	29.667918096073866	28.13490653478818	18.80855406162394
100	22.164053758875475	30.556200882807683	22.118124260655243	25.1616210976616
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	137.0
1	154.5
2	234.5
3	331.0
4	413.0
5	527.5
6	652.0
7	858.0
8	1111.5
9	1218.0
10	1217.5
11	1257.5
12	1367.5
13	1611.0
14	2107.0
15	2616.5
16	2928.5
17	3087.5
18	3091.5
19	2934.0
20	2816.0
21	2936.0
22	3331.5
23	4120.0
24	5354.5
25	6898.5
26	8838.5
27	11097.0
28	13544.5
29	16367.5
30	19143.5
31	21783.0
32	24572.5
33	27388.5
34	29975.0
35	32227.5
36	34769.5
37	37430.5
38	39591.0
39	41378.5
40	43301.0
41	45001.5
42	46197.0
43	48359.5
44	49990.0
45	50595.5
46	50860.5
47	50341.0
48	50194.5
49	49475.5
50	48189.5
51	46758.5
52	44869.5
53	42755.5
54	40102.0
55	37009.0
56	33903.0
57	31083.0
58	27897.5
59	24080.5
60	20294.0
61	16708.0
62	13309.5
63	10516.5
64	8159.5
65	5981.0
66	4365.0
67	3370.5
68	2582.0
69	1899.5
70	1399.5
71	1012.0
72	725.0
73	494.5
74	342.5
75	226.0
76	129.5
77	83.5
78	67.0
79	51.0
80	30.5
81	18.0
82	10.0
83	7.0
84	6.0
85	5.5
86	4.0
87	2.5
88	2.0
89	2.0
90	1.5
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	8.626155365684291E-5
5	0.004399339236498989
6	5.175693219410575E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	9.495057175349968E-4
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	9.146256414383951E-5
50-51	7.381616177180933E-4
52-53	2.3252742428442008E-4
54-55	4.68745678750774E-4
56-57	8.980865556915291E-4
58-59	6.6771020232573E-4
60-61	9.628378297057616E-5
62-63	5.344644544697019E-4
64-65	2.4518613796035537E-4
66-67	4.953145718079823E-4
68-69	7.009153955065315E-4
70-71	2.0248868721015645E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0011680845915736603
90-91	0.0
92-93	0.0
94-95	5.75332945175373E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	18.0
20-21	43.0
22-23	81.0
24-25	85.0
26-27	356.0
28-29	1003.0
30-31	2416.0
32-33	3227.0
34-35	4365.0
36-37	7203.0
38-39	8024.0
40-41	7792.0
42-43	8453.0
44-45	10045.0
46-47	10376.0
48-49	9669.0
50-51	8890.0
52-53	8339.0
54-55	8976.0
56-57	9128.0
58-59	9653.0
60-61	9757.0
62-63	9389.0
64-65	9812.0
66-67	10722.0
68-69	10888.0
70-71	11540.0
72-73	9533.0
74-75	9450.0
76-77	9876.0
78-79	10424.0
80-81	10019.0
82-83	9413.0
84-85	9262.0
86-87	9696.0
88-89	9807.0
90-91	10089.0
92-93	9732.0
94-95	11376.0
96-97	35328.0
98-99	76036.0
100-101	748974.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.90363640100642
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.7107373379488	89.87586278654973
2	3.354504360369876	6.3000031552352675
3	0.5458333966256975	1.5376722243679748
4	0.18797154339537261	0.7060484585894028
5	0.07488214202226781	0.351585271869378
6	0.03998584662429317	0.2252889841556421
7	0.020716075105386058	0.1361720349046479
8	0.014525167396656661	0.10911728303035201
9	0.011263988014908726	0.09519564914795547
>10	0.03839344041919074	0.5938734641196948
>50	0.001186702077520366	0.06918068802996721
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	8.626155365684291E-5	0.0	0.0	0.0	0.0
7	8.626155365684291E-5	0.0	0.0	0.0	0.0
8	8.626155365684291E-5	0.0	0.0	0.0	0.0
9	8.626155365684291E-5	0.0	0.0	0.0	0.0
10-11	8.626155365684291E-5	0.0	0.0	0.0	0.0
12-13	8.626155365684291E-5	0.0	0.0	0.0	0.0
14-15	8.626155365684291E-5	1.7252310731368583E-4	0.0	0.0	0.0
16-17	1.7252310731368583E-4	1.7252310731368583E-4	0.0	0.0	0.0
18-19	1.7252310731368583E-4	1.7252310731368583E-4	0.0	4.3130776828421456E-5	0.0
20-21	1.7252310731368583E-4	1.7252310731368583E-4	0.0	8.626155365684291E-5	0.0
22-23	1.7252310731368583E-4	1.7252310731368583E-4	0.0	8.626155365684291E-5	0.0
24-25	1.7252310731368583E-4	2.156538841421073E-4	0.0	2.156538841421073E-4	0.0
26-27	1.7252310731368583E-4	2.5878466097052877E-4	0.0	3.4504621462737165E-4	0.0
28-29	1.7252310731368583E-4	3.0191543779895024E-4	0.0	9.057463133968506E-4	0.0
30-31	1.7252310731368583E-4	4.7443854511263606E-4	0.0	0.002630977386533709	0.0
32-33	1.7252310731368583E-4	5.175693219410575E-4	0.0	0.004787516227954782	0.0
34-35	1.7252310731368583E-4	5.175693219410575E-4	0.0	0.008971201580311663	0.0
36-37	1.7252310731368583E-4	5.175693219410575E-4	0.0	0.015613341211888569	0.0
38-39	1.7252310731368583E-4	5.175693219410575E-4	0.0	0.03208929796034556	0.0
40-41	1.7252310731368583E-4	5.175693219410575E-4	0.0	0.05029048578193942	0.0
42-43	1.7252310731368583E-4	5.175693219410575E-4	0.0	0.059994910568334246	0.0
44-45	1.7252310731368583E-4	7.763539829115863E-4	0.0	0.07245970507174805	0.0
46-47	1.7252310731368583E-4	0.001035138643882115	0.0	0.0878573923994945	0.0
48-49	1.7252310731368583E-4	0.001035138643882115	0.0	0.10364325671869676	0.0
50-51	1.7252310731368583E-4	0.001121400197538958	0.0	0.12007608269032533	0.0
52-53	1.7252310731368583E-4	0.001121400197538958	0.0	0.151561549775073	0.0
54-55	1.7252310731368583E-4	0.001207661751195801	0.0	0.19145751834136285	0.0
56-57	1.7252310731368583E-4	0.0012939233048526438	0.0	0.2590434456314993	0.0
58-59	1.7252310731368583E-4	0.0012939233048526438	0.0	0.31364700909628085	0.0
60-61	1.7252310731368583E-4	0.0012939233048526438	0.0	0.33818842111165265	0.0
62-63	1.7252310731368583E-4	0.0012939233048526438	0.0	0.3546643778601096	0.0
64-65	1.7252310731368583E-4	0.0014664464121663294	0.0	0.36229852535874024	0.0
66-67	1.7252310731368583E-4	0.0014664464121663294	0.0	0.3679917879000919	0.0
68-69	1.7252310731368583E-4	0.0014664464121663294	0.0	0.37325374267315925	0.0
70-71	1.7252310731368583E-4	0.0014664464121663294	0.0	0.3776962126864867	0.0
72-73	1.7252310731368583E-4	0.0014664464121663294	0.0	0.3824837289144415	0.0
74-75	1.7252310731368583E-4	0.0014664464121663294	0.0	0.3867105450436268	0.0
76-77	1.7252310731368583E-4	0.0014664464121663294	0.0	0.3892983916533321	0.0
78-79	1.7252310731368583E-4	0.0014664464121663294	0.0	0.3913686689410963	0.0
80-81	1.7252310731368583E-4	0.0014664464121663294	0.0	0.3920156305935226	0.0
82-83	1.7252310731368583E-4	0.0014664464121663294	0.0	0.3924038075849784	0.0
84-85	1.7252310731368583E-4	0.0014664464121663294	0.0	0.39253319991546365	0.0
86-87	1.7252310731368583E-4	0.0014664464121663294	0.0	0.39266259224594896	0.0
88	1.7252310731368583E-4	0.0015527079658231725	0.0	0.39266259224594896	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1180	0.0	36.9543	1
GTATCAA	2135	0.0	33.511864	1
TCAACGC	2240	0.0	32.885994	4
ATCAACG	2260	0.0	32.407642	3
CAACGCA	2305	0.0	31.958622	5
AACGCAG	2395	0.0	30.75502	6
TGGACCG	140	1.5643309E-9	30.239996	5
TATCAAC	2525	0.0	28.838774	2
ACGCAGA	2870	0.0	25.516304	7
CGCAGAG	2930	0.0	25.138258	8
CTTACGC	85	0.0012545182	24.903524	3
ACCGACC	135	3.2731376E-5	21.949158	8
ACACCGT	215	6.2918843E-9	21.658407	6
GCAGAGT	3460	0.0	21.042913	9
GGACCGT	165	6.5958575E-6	20.52477	6
GTACATG	2425	0.0	18.680212	1
GACCGTG	205	2.060884E-6	18.584127	7
TACATGG	2415	0.0	18.582258	2
GAGTACT	2135	0.0	18.240784	12-13
TATACTG	650	0.0	18.237043	5
>>END_MODULE