FastQCFastQC Report
Fri 27 May 2016
SRR2139746_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139746_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10448131
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96250.043.4959261
GTATCAA160300.032.3730961
TCAACGC180700.027.8430864
ATCAACG184000.027.2986243
GACCGAG110200.027.22068890-91
CAACGCA186650.026.9336015
GAGACCG111150.026.6975888-89
ACCGAGG112950.026.5409592-93
AGACCGA107200.026.4635890-91
AACGCAG192600.026.1667396
ACGAGAC114350.025.99022186-87
CGAGACC113050.025.25072188-89
TATCAAC203650.025.0529842
CCACGAG121500.024.30277484-85
GCCCACG122650.024.28809482-83
CACGAGA118050.023.98641686-87
CCCACGA117500.023.78384684-85
CCGAGCC131500.022.1885778-79
ATCTCCG128300.022.11666174-75
ACGCAGA229700.021.9585347
Produced by FastQC (version 0.11.5)