##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2139746_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10448131 Sequences flagged as poor quality 0 Sequence length 19-101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.815459051958673 31.0 31.0 34.0 30.0 34.0 2 32.0030852407957 33.0 31.0 34.0 30.0 34.0 3 32.069850387595636 33.0 31.0 34.0 30.0 34.0 4 35.75752447973709 37.0 35.0 37.0 35.0 37.0 5 35.51792143494372 37.0 35.0 37.0 33.0 37.0 6 35.54929537158368 37.0 35.0 37.0 33.0 37.0 7 35.508263056808914 37.0 35.0 37.0 33.0 37.0 8 35.49924823875198 37.0 35.0 37.0 33.0 37.0 9 37.27744818666611 39.0 37.0 39.0 34.0 39.0 10-11 37.12997731364585 39.0 37.0 39.0 33.0 39.0 12-13 37.1392422721346 39.0 37.0 39.0 33.0 39.0 14-15 38.270606915246375 40.0 38.0 41.0 33.5 41.0 16-17 38.20078356597941 40.0 38.0 41.0 33.0 41.0 18-19 38.26131113784848 40.0 38.0 41.0 33.5 41.0 20-21 38.30195909727 40.0 38.0 41.0 34.0 41.0 22-23 38.28786570290833 40.0 38.0 41.0 34.0 41.0 24-25 38.24452648983073 40.0 38.0 41.0 34.0 41.0 26-27 38.13132580325701 40.0 38.0 41.0 33.5 41.0 28-29 37.96512725442845 40.0 37.0 41.0 33.0 41.0 30-31 37.872871117545444 40.0 37.0 41.0 33.0 41.0 32-33 37.78609818597952 40.0 37.0 41.0 33.0 41.0 34-35 37.71971577809677 39.0 37.0 41.0 33.0 41.0 36-37 37.68032842654041 39.0 37.0 41.0 33.0 41.0 38-39 37.59680589572076 39.0 37.0 41.0 32.5 41.0 40-41 37.58218055291227 39.0 37.0 41.0 32.5 41.0 42-43 37.532719541287385 39.0 37.0 41.0 32.0 41.0 44-45 37.46527044009311 39.0 37.0 41.0 32.0 41.0 46-47 37.41535576397351 39.0 36.0 40.5 32.0 41.0 48-49 37.43254583496995 39.0 36.0 40.0 32.0 41.0 50-51 37.33095035612463 39.0 36.0 40.0 31.5 41.0 52-53 37.1490347015734 39.0 36.0 40.0 31.0 41.0 54-55 36.96394831325976 39.0 35.5 40.0 31.0 41.0 56-57 36.85058930279246 39.0 35.0 40.0 31.0 41.0 58-59 36.66718945155719 38.5 35.0 40.0 30.5 41.0 60-61 36.497878104064554 38.0 35.0 40.0 30.0 41.0 62-63 36.358870299362124 38.0 35.0 40.0 30.0 41.0 64-65 36.11823228444644 38.0 34.0 40.0 30.0 41.0 66-67 35.98843822131674 37.0 34.0 40.0 30.0 41.0 68-69 35.722139980202996 37.0 34.0 39.5 29.5 41.0 70-71 35.40615585973757 36.0 34.0 39.0 29.0 40.5 72-73 35.10581722614654 36.0 34.0 39.0 29.0 40.0 74-75 34.75444501746801 35.5 33.5 38.5 29.0 40.0 76-77 32.59990964488961 33.5 30.5 36.0 26.5 38.5 78-79 33.875812666184586 35.0 32.5 37.0 28.5 39.0 80-81 33.861697001920305 35.0 33.0 36.5 29.0 39.0 82-83 33.669462055479414 35.0 33.0 36.0 29.0 38.5 84-85 33.47165640878808 35.0 33.0 36.0 29.0 37.0 86-87 33.22666627242636 35.0 32.5 35.0 29.0 37.0 88-89 33.0659375679781 35.0 32.0 35.0 29.0 36.5 90-91 32.89572530056046 34.0 32.0 35.0 28.0 36.0 92-93 32.860362785194326 34.0 32.0 35.0 29.0 36.0 94-95 32.818718727512504 34.0 32.0 35.0 29.0 36.0 96-97 32.83797138124761 34.0 32.0 35.0 29.0 35.0 98-99 32.99245324406362 34.0 32.0 35.0 29.0 35.0 100-101 31.825280175793896 33.0 30.5 34.5 26.5 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 1.0 19 12.0 20 51.0 21 333.0 22 1504.0 23 5312.0 24 14364.0 25 30991.0 26 56884.0 27 91684.0 28 135587.0 29 185422.0 30 242973.0 31 311110.0 32 394975.0 33 517245.0 34 700989.0 35 990023.0 36 1517853.0 37 2281148.0 38 2233237.0 39 729404.0 40 7028.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.22836897814547 20.69472520970497 13.656710468121044 24.420195344028517 2 14.997290891624468 21.83581549426225 40.172387538503465 22.99450607560982 3 18.36352220640862 25.925253466894972 30.586498419331342 25.124725907365065 4 12.618381220526429 17.936078711111104 38.75914266388888 30.68639740447359 5 12.909544578790655 37.974403075000026 36.15918829493891 12.95686405127042 6 30.30418550456536 38.21841437478148 18.562018412671126 12.915381707982029 7 26.51395034326717 32.5112149255417 23.393870482086268 17.580964249104863 8 24.369124484830618 37.03038470447032 21.414449819200193 17.186040991498867 9 25.348839902562474 16.51459959680827 21.360365791738253 36.776194708891 10-11 23.975420976223802 26.9079983568298 29.37083943332833 19.745741233618066 12-13 24.552222027928355 24.338790693917193 29.485482084717678 21.623505193436774 14-15 21.603232195308426 26.000291343973387 26.772309803542854 25.624166657175333 16-17 21.53401510442466 29.115826032226533 27.562768286632718 21.787390576716092 18-19 21.508743684906424 28.493101151038847 28.94414080821617 21.05401435583856 20-21 22.150273871039314 27.752790157526817 28.857329010306465 21.239606961127407 22-23 21.84654660311983 27.680599796984012 28.706348404073033 21.766505195823125 24-25 21.686632549323846 27.747103979540128 28.786188436600174 21.78007503453585 26-27 21.702572002530964 27.910683368661456 28.67600174586713 21.71074288294045 28-29 21.66610053346571 28.032158538641095 28.63447090239108 21.667270025502116 30-31 21.880615143324032 27.894453895141414 28.58379445784589 21.641136503688667 32-33 21.67844402976472 27.98825348999775 28.561683063811564 21.771619416425967 34-35 21.82734446921156 28.012587123892622 28.54841569932655 21.611652707569267 36-37 21.782818672517976 27.95224538203986 28.529916572204527 21.735019373237638 38-39 21.62887273972938 28.08569331277986 28.602211945484353 21.68322200200641 40-41 21.8689302970423 27.94976867281747 28.487716268906226 21.693584761233996 42-43 21.786851593108306 28.01646932103223 28.337143852275727 21.859535233583742 44-45 21.809947049458835 28.072824665379798 28.232587107104134 21.88464117805723 46-47 21.845352438164316 28.064463560048214 28.046515686020136 22.043668315767334 48-49 21.761384745265424 28.151481772839116 28.11641150356225 21.970721978333213 50-51 21.747862203381775 28.22863456593047 28.05149672385899 21.972006506828766 52-53 21.921031729313345 28.156358567363714 27.88336633545967 22.039243367863275 54-55 21.862459045010027 28.05752095065111 27.98958778400294 22.090432220335927 56-57 21.844303875404552 28.090223551173704 27.966891618771765 22.098580954649975 58-59 21.83481652560136 28.101679185274236 27.970913725544545 22.092590563579858 60-61 21.795615776934056 27.910577399846538 28.129757491595807 22.164049331623602 62-63 21.960882746944353 28.02610934210629 28.101647863554017 21.911360047395334 64-65 21.677707847693974 28.00231700751817 28.030177566564024 22.289797578223837 66-67 21.712654669504754 28.124087039663813 27.878833642144365 22.28442464868707 68-69 21.730486994003137 28.398637621148325 27.843241061358327 22.027634323490204 70-71 21.72734128849496 28.194827384565883 27.862284594184562 22.215546732754593 72-73 21.735635228320373 28.17569103290608 27.601913545769825 22.486760193003718 74-75 21.729226230335033 28.2264443104975 27.580189454257493 22.46414000490997 76-77 21.86553725620872 27.91657746784483 27.74225229527538 22.475632980671058 78-79 21.869487724029028 27.90634664230444 27.6989423183777 22.52522331528883 80-81 22.137984775936648 27.93722940593447 27.446950600566765 22.477835217562117 82-83 22.15747177158511 28.237044347753194 27.492696752723987 22.112787127937708 84-85 21.921391233683156 27.936014068701397 27.45853452782197 22.684060169793476 86-87 21.827694751186392 28.26011468079408 27.538115149397978 22.374075418621548 88-89 22.15786330425744 27.96443499538211 27.502646110977725 22.375055589382722 90-91 22.224420724472875 28.249790810373103 27.5457930354284 21.97999542972562 92-93 21.885096458155598 28.19009549495005 27.556581445909128 22.36822660098522 94-95 22.131692761375195 27.94682807161572 27.63292033861661 22.28855882839247 96-97 22.076447597841224 28.224593723020945 27.761698781704098 21.93725989743374 98-99 22.345444097732077 28.549872277766582 27.96913370498374 21.1355499195176 100-101 26.016472182212606 35.72124161962996 28.680369517637132 9.581916680520305 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2124.0 1 1845.0 2 2216.5 3 3092.5 4 4047.0 5 6181.5 6 8145.5 7 9574.0 8 10542.0 9 10642.0 10 10069.0 11 9395.5 12 10135.0 13 13226.0 14 20313.0 15 29624.0 16 37092.0 17 41773.0 18 44143.0 19 44392.5 20 43759.0 21 43891.5 22 45802.0 23 51782.0 24 63083.5 25 78994.0 26 99181.5 27 121783.5 28 146683.0 29 173599.5 30 199206.5 31 223458.5 32 248769.0 33 274239.0 34 297827.5 35 319539.5 36 342554.5 37 363156.0 38 380820.5 39 397903.0 40 412529.5 41 425508.5 42 435076.0 43 443544.0 44 451496.0 45 456915.5 46 457799.5 47 455928.0 48 452649.0 49 443490.5 50 431263.0 51 415905.5 52 395284.0 53 371960.0 54 347393.5 55 320916.0 56 292223.5 57 262862.0 58 233360.0 59 202024.0 60 168825.0 61 137173.5 62 109101.5 63 85000.0 64 64232.0 65 48373.0 66 36666.0 67 27875.0 68 21211.5 69 15483.5 70 11321.0 71 8288.0 72 5890.5 73 4142.5 74 2923.0 75 2062.5 76 1449.0 77 1017.5 78 703.0 79 501.0 80 357.5 81 255.5 82 199.5 83 144.0 84 93.0 85 59.5 86 43.5 87 35.0 88 25.0 89 17.5 90 12.5 91 8.0 92 5.5 93 2.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.211279510182252E-4 3 2.009928857132438E-4 4 0.0 5 9.571089795868753E-6 6 0.0 7 9.571089795868753E-6 8 0.0010623909673414316 9 0.0 10-11 3.349881428554064E-4 12-13 3.8284359183475016E-4 14-15 0.0 16-17 4.785544897934376E-6 18-19 4.5462676530376586E-4 20-21 9.571558840344387E-6 22-23 0.0 24-25 1.9628724119527248E-4 26-27 0.0 28-29 4.505399841534543E-4 30-31 4.320360905668616E-4 32-33 0.001034843518183019 34-35 0.0012704638883924045 36-37 3.399664511392689E-5 38-39 0.0017519084301931534 40-41 3.8470125949712733E-4 42-43 1.0943322889258099E-4 44-45 1.6076192309641933E-4 46-47 5.589651867334999E-5 48-49 1.5417759614514896E-5 50-51 1.0389315171148125E-5 52-53 1.0493242745168714E-5 54-55 4.2381894242869976E-4 56-57 6.638764581591327E-4 58-59 5.521610881254511E-4 60-61 6.375750785525193E-4 62-63 9.925483878367386E-5 64-65 8.927869953075116E-5 66-67 1.4129758888663943E-4 68-69 1.3178010049435873E-4 70-71 1.394356169111002E-4 72-73 0.0 74-75 1.1940593636165098E-5 76-77 9.141786628399511E-4 78-79 2.455868955814865E-5 80-81 1.8072809115924918E-4 82-83 0.0 84-85 1.2842028981505745E-5 86-87 1.3042132936504701E-5 88-89 1.9891828238041532E-5 90-91 1.0126151596590321E-4 92-93 6.880034895536991E-5 94-95 3.3714887525028035E-4 96-97 2.8823288525369576E-5 98-99 7.594828073978182E-6 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 341.0 20-21 945.0 22-23 2007.0 24-25 4005.0 26-27 6373.0 28-29 12594.0 30-31 26014.0 32-33 34547.0 34-35 48815.0 36-37 73691.0 38-39 80644.0 40-41 84063.0 42-43 94437.0 44-45 111395.0 46-47 112494.0 48-49 105466.0 50-51 98585.0 52-53 89646.0 54-55 97749.0 56-57 101830.0 58-59 59346.0 60-61 109284.0 62-63 105669.0 64-65 112123.0 66-67 119406.0 68-69 120712.0 70-71 118112.0 72-73 114159.0 74-75 117539.0 76-77 112587.0 78-79 120146.0 80-81 121071.0 82-83 116358.0 84-85 117998.0 86-87 124604.0 88-89 132482.0 90-91 138311.0 92-93 144819.0 94-95 166526.0 96-97 249852.0 98-99 922398.0 100-101 5818988.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.1344890504368 #Duplication Level Percentage of deduplicated Percentage of total 1 90.91962612252897 79.22235166843308 2 6.462102977618567 11.261440822922001 3 1.5418841324399615 4.030538581653962 4 0.5245406702871723 1.8282233316658563 5 0.22422679579320348 0.9768943641428711 6 0.11321972952516991 0.59192059695626 7 0.06316584826987402 0.38527467401030235 8 0.04260616755181365 0.2969973312019666 9 0.026454414440037346 0.2074582696825036 >10 0.08079676978444675 1.0693180527061381 >50 0.0010343595663520845 0.05808677072272715 >100 3.258564927828004E-4 0.05919207355497747 >500 1.6155701598523192E-5 0.012303462347297744 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.914217959173751E-5 0.0 9.571089795868754E-6 0.0 3 0.0 1.914217959173751E-5 0.0 9.571089795868754E-6 0.0 4 0.0 2.871326938760626E-5 0.0 1.914217959173751E-5 0.0 5 0.0 2.871326938760626E-5 0.0 2.871326938760626E-5 0.0 6 4.785544897934377E-5 2.871326938760626E-5 0.0 2.871326938760626E-5 9.571089795868754E-6 7 4.785544897934377E-5 2.871326938760626E-5 0.0 2.871326938760626E-5 9.571089795868754E-6 8 5.742653877521252E-5 3.828435918347502E-5 0.0 2.871326938760626E-5 1.914217959173751E-5 9 5.742653877521252E-5 8.613980816281878E-5 0.0 2.871326938760626E-5 1.914217959173751E-5 10-11 5.742653877521252E-5 8.613980816281878E-5 0.0 3.828435918347502E-5 1.914217959173751E-5 12-13 5.742653877521252E-5 8.613980816281878E-5 0.0 5.742653877521252E-5 1.914217959173751E-5 14-15 6.699762857108128E-5 1.3399525714216255E-4 0.0 7.178317346901566E-5 2.3927724489671885E-5 16-17 1.2920971224422817E-4 1.3399525714216255E-4 0.0 1.052819877545563E-4 3.828435918347502E-5 18-19 1.3399525714216255E-4 1.3878080204009693E-4 0.0 1.435663469380313E-4 3.828435918347502E-5 20-21 1.3399525714216255E-4 1.5313743673390007E-4 0.0 2.0577843061117822E-4 3.828435918347502E-5 22-23 1.435663469380313E-4 1.7706516122357194E-4 0.0 2.8234714897812824E-4 3.828435918347502E-5 24-25 1.435663469380313E-4 2.3927724489671882E-4 0.0 4.1155686122235644E-4 3.828435918347502E-5 26-27 1.435663469380313E-4 2.679905142843251E-4 0.0 6.460485612211409E-4 3.828435918347502E-5 28-29 1.435663469380313E-4 2.679905142843251E-4 0.0 0.0020721409408055854 4.785544897934377E-5 30-31 1.5313743673390007E-4 3.445592326512751E-4 0.0 0.007130461897922222 5.742653877521252E-5 32-33 1.5313743673390007E-4 3.6370141224301264E-4 0.0 0.016098573036651243 5.742653877521252E-5 34-35 1.6749407142770318E-4 4.259134959161596E-4 0.0 0.028287355891690104 6.699762857108128E-5 36-37 1.866362510194407E-4 4.8812557958930646E-4 0.0 0.04492669550180793 6.699762857108128E-5 38-39 2.0099288571324384E-4 5.072677591810439E-4 0.0 0.0768989209649075 6.699762857108128E-5 40-41 2.0099288571324384E-4 5.934075673438628E-4 0.0 0.14252309815028164 6.699762857108128E-5 42-43 2.0099288571324384E-4 6.125497469356003E-4 0.0 0.16915465550728642 6.699762857108128E-5 44-45 2.105639755091126E-4 6.60405195914944E-4 0.0 0.2086593286397347 6.699762857108128E-5 46-47 2.2013506530498133E-4 6.98689555098419E-4 0.0 0.2548111236354138 7.178317346901566E-5 48-49 2.2013506530498133E-4 6.98689555098419E-4 0.0 0.2909084888005328 7.656871836695003E-5 50-51 2.5841942448845634E-4 7.752582734653691E-4 0.0 0.326776147810551 7.656871836695003E-5 52-53 2.7756160408019386E-4 7.944004530571066E-4 0.0 0.3760194048102957 9.092535306075316E-5 54-55 2.7756160408019386E-4 7.944004530571066E-4 0.0 0.4797843748322068 1.052819877545563E-4 56-57 2.7756160408019386E-4 7.944004530571066E-4 0.0 0.5912110022357109 1.052819877545563E-4 58-59 2.8234714897812824E-4 8.039715428529754E-4 0.0 0.6944926322229306 1.052819877545563E-4 60-61 3.0627487346780014E-4 8.278992673426473E-4 0.0 1.1868151346877256 1.052819877545563E-4 62-63 3.2541705305953766E-4 8.566125367302534E-4 0.0 1.1993724044999055 1.052819877545563E-4 64-65 3.30202597957472E-4 9.475378897910066E-4 0.0 1.212145023832492 1.052819877545563E-4 66-67 3.445592326512751E-4 9.618945244848098E-4 0.0 1.2206680792957132 1.052819877545563E-4 68-69 3.445592326512751E-4 9.714656142806785E-4 0.0 1.228344093312 1.052819877545563E-4 70-71 3.493447775492095E-4 9.762511591786129E-4 0.0 1.2359722518793075 1.244241673462938E-4 72-73 3.732725020388814E-4 9.762511591786129E-4 0.0 1.2419541830017253 1.8185070612150632E-4 74-75 3.732725020388814E-4 9.906077938724158E-4 0.0 1.246801939983333 1.8185070612150632E-4 76-77 3.780580469368158E-4 0.0010145355183620878 0.0 1.2512668533731057 1.8185070612150632E-4 78-79 3.8284359183475016E-4 0.0010336776979538254 0.0 1.2553010677220644 1.8185070612150632E-4 80-81 3.8284359183475016E-4 0.0010384632428517599 0.0 1.2573014254894008 1.9142179591737508E-4 82-83 3.8284359183475016E-4 0.0010432487877496941 0.0 1.2580049005893974 2.105639755091126E-4 84-85 3.8284359183475016E-4 0.0010528198775455629 0.0 1.258909368575107 2.1534952040704698E-4 86-87 3.8762913673268454E-4 0.0010623909673414316 0.0 1.2592395711730644 2.2013506530498133E-4 88-89 3.924146816306189E-4 0.001105460871422841 0.0 1.2593544242506147 2.2013506530498133E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9625 0.0 43.495926 1 GTATCAA 16030 0.0 32.373096 1 TCAACGC 18070 0.0 27.843086 4 ATCAACG 18400 0.0 27.298624 3 GACCGAG 11020 0.0 27.220688 90-91 CAACGCA 18665 0.0 26.933601 5 GAGACCG 11115 0.0 26.69758 88-89 ACCGAGG 11295 0.0 26.54095 92-93 AGACCGA 10720 0.0 26.46358 90-91 AACGCAG 19260 0.0 26.166739 6 ACGAGAC 11435 0.0 25.990221 86-87 CGAGACC 11305 0.0 25.250721 88-89 TATCAAC 20365 0.0 25.052984 2 CCACGAG 12150 0.0 24.302774 84-85 GCCCACG 12265 0.0 24.288094 82-83 CACGAGA 11805 0.0 23.986416 86-87 CCCACGA 11750 0.0 23.783846 84-85 CCGAGCC 13150 0.0 22.18857 78-79 ATCTCCG 12830 0.0 22.116661 74-75 ACGCAGA 22970 0.0 21.958534 7 >>END_MODULE