FastQCFastQC Report
Fri 27 May 2016
SRR2139745_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139745_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8461392
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85950.041.928831
TATCTCG132200.039.79514394-95
CCGTCTT52150.035.20377392-93
TGCCGTC54050.032.93616590-91
TATGCCG53850.032.3265288-89
GCCGTCT53250.031.62547190-91
ATGCCGT54500.030.16647388-89
GTATCAA137050.029.7625711
CGTATGC64100.027.73227786-87
TCAACGC154200.025.5795964
CTCGTAT68550.025.365684-85
CGCTATC177800.025.2993592-93
GCTATCT202150.025.2783494-95
ATCAACG157150.025.1261753
CAACGCA159650.024.6538475
TCGTATG68700.024.00252384-85
AACGCAG164300.023.9821156
ATCTCGT101600.023.97493494-95
TATCAAC171500.023.2691142
TCTCGTA70550.022.91187582-83
Produced by FastQC (version 0.11.5)