##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139745_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8461392
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.85998001274495	31.0	31.0	34.0	30.0	34.0
2	32.05072841442637	33.0	31.0	34.0	30.0	34.0
3	32.118109408002844	34.0	31.0	34.0	30.0	34.0
4	35.79981308040095	37.0	35.0	37.0	35.0	37.0
5	35.55055291138858	37.0	35.0	37.0	33.0	37.0
6	35.582063211348675	37.0	35.0	37.0	33.0	37.0
7	35.54525744700163	37.0	35.0	37.0	33.0	37.0
8	35.539391981839394	37.0	35.0	37.0	33.0	37.0
9	37.333035628180326	39.0	37.0	39.0	34.0	39.0
10-11	37.185740833186784	39.0	37.0	39.0	33.5	39.0
12-13	37.19545661044897	39.0	37.0	39.0	33.0	39.0
14-15	38.332611820844605	40.0	38.0	41.0	33.5	41.0
16-17	38.2659481442297	40.0	38.0	41.0	33.0	41.0
18-19	38.32172430966442	40.0	38.0	41.0	33.5	41.0
20-21	38.35964680942122	40.0	38.0	41.0	34.0	41.0
22-23	38.34829221686856	40.0	38.0	41.0	34.0	41.0
24-25	38.300445954176865	40.0	38.0	41.0	34.0	41.0
26-27	38.187779691500374	40.0	38.0	41.0	34.0	41.0
28-29	38.018099729582026	40.0	37.0	41.0	33.0	41.0
30-31	37.93032049429584	40.0	37.0	41.0	33.0	41.0
32-33	37.84700977174636	40.0	37.0	41.0	33.0	41.0
34-35	37.780492802275916	40.0	37.0	41.0	33.0	41.0
36-37	37.73787217602835	40.0	37.0	41.0	33.0	41.0
38-39	37.65084836977722	39.5	37.0	41.0	33.0	41.0
40-41	37.62933814691911	39.0	37.0	41.0	33.0	41.0
42-43	37.5716095970439	39.0	37.0	41.0	32.5	41.0
44-45	37.49761283890107	39.0	37.0	41.0	32.0	41.0
46-47	37.43869539825353	39.0	36.0	41.0	32.0	41.0
48-49	37.44548601610313	39.0	36.0	41.0	32.0	41.0
50-51	37.33178291558983	39.0	36.0	40.0	31.5	41.0
52-53	37.13329663999165	39.0	36.0	40.0	31.0	41.0
54-55	36.94372140634975	39.0	35.0	40.0	31.0	41.0
56-57	36.82571696571985	39.0	35.0	40.0	31.0	41.0
58-59	36.63732570196352	38.5	35.0	40.0	30.5	41.0
60-61	36.45216671940937	38.0	35.0	40.0	30.0	41.0
62-63	36.29504570821526	38.0	34.5	40.0	30.0	41.0
64-65	36.042895700267366	37.5	34.0	40.0	30.0	41.0
66-67	35.88457413746255	37.0	34.0	40.0	30.0	41.0
68-69	35.5946473364756	36.5	34.0	39.0	29.5	41.0
70-71	35.25857918679233	36.0	34.0	39.0	29.0	40.5
72-73	34.968643091640374	36.0	34.0	39.0	29.0	40.0
74-75	34.6119283485412	35.0	33.5	38.0	29.0	40.0
76-77	32.464803201164614	33.5	30.5	35.5	25.5	38.5
78-79	33.69232385915175	35.0	32.5	36.0	28.0	39.0
80-81	33.65595361946556	35.0	33.0	36.0	28.0	39.0
82-83	33.47452188501752	35.0	33.0	36.0	29.0	37.0
84-85	33.29937811691215	35.0	33.0	35.5	29.0	37.0
86-87	33.07405739522554	35.0	32.0	35.0	28.0	36.5
88-89	32.91862883752867	34.0	32.0	35.0	28.0	36.0
90-91	32.794196636744076	34.0	32.0	35.0	27.5	36.0
92-93	32.79209259504553	34.0	32.0	35.0	28.5	36.0
94-95	32.75376474301048	34.0	32.0	35.0	29.0	35.0
96-97	32.76118978147416	34.0	32.0	35.0	29.0	35.0
98-99	32.919883058704805	34.0	32.0	35.0	29.0	35.0
100-101	31.664032821486472	33.0	29.5	34.5	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	0.0
19	8.0
20	50.0
21	271.0
22	1250.0
23	4116.0
24	11338.0
25	24874.0
26	46156.0
27	75339.0
28	111002.0
29	152307.0
30	198189.0
31	251596.0
32	321564.0
33	419114.0
34	573195.0
35	815296.0
36	1266406.0
37	1895160.0
38	1754584.0
39	534010.0
40	5565.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.7726066822102	20.235169343294814	13.430154281943206	24.56206969255177
2	15.329016755281554	21.428363761166683	39.70953982186256	23.5330796616892
3	18.661119573099764	25.578192557042783	30.225431632331794	25.535256237525655
4	12.981718963229568	17.506625092730026	37.98899022631149	31.522665717728916
5	13.3527674879027	37.751979816087	35.646841559875725	13.248411136134575
6	31.043544608263034	37.35119469704276	18.286541977962965	13.318718716731242
7	27.147738137587325	32.146089472297106	22.93675152664042	17.769420863475148
8	24.96798665455665	36.43093301691285	21.047224928985624	17.553855399544872
9	25.731463570060342	16.273374404589696	20.97736400819156	37.017798017158405
10-11	24.58875308674877	26.400197225733113	28.853895477097947	20.157154210420167
12-13	25.078165713617466	23.90225413779987	28.985219321667387	22.03436082691528
14-15	22.180199320643656	25.45417027447554	26.185976620984857	26.179653783895944
16-17	22.147994215844992	28.570383488342316	26.97565760903511	22.305964686777582
18-19	22.182876017914623	27.95928501053998	28.321378294529758	21.53646067701564
20-21	22.801102047671325	27.212785237449527	28.200537597490218	21.785575117388927
22-23	22.575411418828764	27.068323468863802	28.05629130618563	22.299973806121802
24-25	22.401689204247795	27.195192876001705	28.088583435200054	22.314534484550446
26-27	22.327404450358543	27.35275523028018	28.04172395948843	22.278116359872847
28-29	22.34413582804395	27.386188495384566	28.028360247829596	22.24131542874189
30-31	22.485314940623905	27.291915389916138	27.936251513534767	22.28651815592519
32-33	22.253638747147836	27.45069111994183	27.944701604062356	22.350968528847982
34-35	22.433612432407717	27.416711152098866	27.938465274677593	22.211211140815827
36-37	22.437140501653378	27.313068065710866	27.892685072797864	22.357106359837896
38-39	22.308215522807508	27.46521230716513	27.96342952209736	22.263142647929993
40-41	22.433679620878927	27.20343184040452	27.96405030794822	22.39883823076833
42-43	22.45652762755182	27.265105463229293	27.845412728801556	22.432954180417333
44-45	22.30931155219753	27.305769478705304	27.72865297346181	22.65626599563535
46-47	22.395019678172442	27.265612876064193	27.58384221768551	22.755525228077854
48-49	22.217809861060136	27.553431500187703	27.64533359164266	22.5834250471095
50-51	22.19008795866941	27.61966262702439	27.624757848630594	22.56549156567561
52-53	22.301581546142053	27.472650101069984	27.2203293276451	23.005439025142866
54-55	22.266014537372783	27.128866283951464	27.462323232637353	23.1427959460384
56-57	22.21133510696488	26.80116799619821	27.870245801662037	23.117251095174872
58-59	22.04293591365247	26.555258091701354	27.994775623179407	23.40703037146676
60-61	22.118403767327337	26.257099468588095	28.356342909798325	23.26815385428624
62-63	21.848454337877524	26.939025261882076	28.323870140785935	22.88865025945446
64-65	21.360243947796583	27.240667880717222	28.03470455781185	23.364383613674338
66-67	21.476223994544025	27.98543274993811	27.407409114106624	23.130934141411238
68-69	21.59920140352382	28.382439345737065	26.817723914810877	23.200635335928236
70-71	21.579808058422095	27.97627299721474	26.778534956603238	23.665383987759924
72-73	21.473924761434354	27.483944903999042	26.670433421280077	24.37169691328653
74-75	22.043691614983093	27.01142676074293	26.800974011137153	24.143907613136825
76-77	22.597306517541014	26.8246675727774	26.6568626387708	23.92116327091078
78-79	22.850988831618864	26.641973414200425	25.797231716013663	24.70980603816705
80-81	22.698862334639635	27.19482392745663	25.73708870967077	24.369225028232965
82-83	22.585795042463943	27.396023862249603	25.843486285574052	24.174694809712406
84-85	22.581634948994132	27.392844521540155	25.80245029568467	24.223070233781044
86-87	23.005374171894754	27.83638399956493	25.706826242758098	23.45141558578222
88-89	23.20940131615292	27.408169460464926	26.182581700320934	23.19984752306122
90-91	22.827936659540306	27.40782611896517	26.51743276403884	23.246804457455692
92-93	22.59332935718546	26.76879126461187	26.4143432350268	24.223536143175874
94-95	22.413997907325683	26.77234174192907	27.275740895288564	23.537919455456684
96-97	21.8714566386428	27.101056219482505	27.576827543674508	23.45065959820019
98-99	22.198799898228348	27.033439769063122	28.05381712976959	22.713943202938935
100-101	26.68479321860064	34.39022409524192	28.81931326733617	10.105669418821268
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1572.0
1	1372.5
2	1742.0
3	2449.5
4	3164.5
5	4865.0
6	6388.0
7	7465.0
8	8250.0
9	8234.5
10	7541.0
11	6802.5
12	7430.0
13	9891.0
14	15171.5
15	22253.5
16	27976.0
17	31520.0
18	33028.0
19	32481.5
20	31115.5
21	30214.5
22	30401.5
23	33386.5
24	39542.5
25	48839.0
26	61681.0
27	76406.5
28	93273.5
29	112081.5
30	130475.0
31	148353.0
32	166520.0
33	184385.5
34	201711.5
35	219031.5
36	238262.0
37	256744.5
38	273626.0
39	289913.0
40	305886.5
41	321781.5
42	333417.5
43	343583.5
44	354005.0
45	362738.5
46	370016.0
47	373855.5
48	375422.0
49	374478.5
50	370391.0
51	363399.0
52	351203.0
53	336159.0
54	319089.5
55	299255.0
56	276980.5
57	251016.0
58	223266.0
59	193416.0
60	161282.0
61	131563.5
62	105568.0
63	82257.5
64	62070.0
65	46594.5
66	35121.0
67	26717.0
68	20480.0
69	15249.0
70	11078.5
71	8026.0
72	5855.0
73	4188.5
74	2972.0
75	2102.5
76	1470.5
77	980.5
78	660.5
79	455.0
80	307.0
81	218.5
82	176.5
83	134.0
84	97.5
85	75.0
86	60.0
87	48.0
88	33.0
89	19.5
90	13.5
91	9.0
92	7.5
93	4.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	7.09103183022368E-4
3	2.8364127320894717E-4
4	1.1818386383706132E-5
5	0.0
6	0.0
7	2.3636772767412264E-5
8	9.100157515453722E-4
9	0.0
10-11	2.540953072496819E-4
12-13	4.904630349238045E-4
14-15	5.909193191853066E-6
16-17	5.909193191853066E-6
18-19	4.3137110300527385E-4
20-21	0.0
22-23	0.0
24-25	1.773476873033953E-4
26-27	0.0
28-29	5.504400472242048E-4
30-31	3.616522027524223E-4
32-33	0.0012070935476687485
34-35	0.0012238070224673656
36-37	4.803967789155775E-5
38-39	0.0019372787831951962
40-41	5.478175981655658E-4
42-43	2.0268946812625194E-4
44-45	1.8613068309712523E-4
46-47	5.647701642715601E-5
48-49	5.07816244152972E-5
50-51	5.775436772218763E-5
52-53	1.2938198173475748E-5
54-55	3.952047539633368E-4
56-57	6.228997410424097E-4
58-59	4.808961408604584E-4
60-61	6.852249511940371E-4
62-63	1.3211503890589725E-4
64-65	8.693116923760028E-5
66-67	2.5073356509246647E-4
68-69	1.7183844244536E-4
70-71	1.952606338941219E-4
72-73	0.0
74-75	2.1521615521503896E-5
76-77	8.880029563219733E-4
78-79	2.9537963482954198E-5
80-81	2.399768242381992E-4
82-83	2.285013548226163E-5
84-85	3.0938653214127734E-5
86-87	1.5717856923649175E-5
88-89	4.796881259680207E-5
90-91	9.77254085410704E-5
92-93	8.305551954175945E-5
94-95	2.8848005796752225E-4
96-97	6.968578592021344E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	264.0
20-21	827.0
22-23	1565.0
24-25	3222.0
26-27	5462.0
28-29	11124.0
30-31	23366.0
32-33	30639.0
34-35	42717.0
36-37	64027.0
38-39	46721.0
40-41	72375.0
42-43	77917.0
44-45	89798.0
46-47	92290.0
48-49	86893.0
50-51	80324.0
52-53	11311.0
54-55	11848.0
56-57	11640.0
58-59	12436.0
60-61	92697.0
62-63	91204.0
64-65	96581.0
66-67	103803.0
68-69	104638.0
70-71	102257.0
72-73	98687.0
74-75	101848.0
76-77	96192.0
78-79	103479.0
80-81	103727.0
82-83	100103.0
84-85	101004.0
86-87	106709.0
88-89	112657.0
90-91	118718.0
92-93	123981.0
94-95	141620.0
96-97	210171.0
98-99	810304.0
100-101	4764246.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.7143924925509
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.0267922141909	77.16591802692486
2	7.04187850749448	12.071806765524808
3	1.6490112507587003	4.240319927164905
4	0.6062133922329197	2.078448505443728
5	0.2720141349740458	1.165776316434354
6	0.13308796927222333	0.6844532660541545
7	0.08187753698349527	0.4912658338928666
8	0.04943303448123575	0.33896980156979556
9	0.03505606278127828	0.27043282120301926
>10	0.1025263984563902	1.3446028129184417
>50	0.0017397416233803243	0.0949769427380452
>100	3.69756750949731E-4	0.05302898013105597
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.3636772767412264E-5	0.0	0.0	0.0
3	0.0	2.3636772767412264E-5	0.0	0.0	0.0
4	0.0	7.091031830223679E-5	0.0	0.0	0.0
5	1.1818386383706132E-5	7.091031830223679E-5	0.0	1.1818386383706132E-5	0.0
6	7.091031830223679E-5	7.091031830223679E-5	0.0	1.1818386383706132E-5	0.0
7	7.091031830223679E-5	7.091031830223679E-5	0.0	1.1818386383706132E-5	0.0
8	7.091031830223679E-5	1.1818386383706132E-4	0.0	2.3636772767412264E-5	1.1818386383706132E-5
9	7.091031830223679E-5	2.1273095490671038E-4	0.0	5.909193191853066E-5	1.1818386383706132E-5
10-11	7.681951149408986E-5	2.1273095490671038E-4	0.0	1.1818386383706132E-4	1.1818386383706132E-5
12-13	9.454709106964906E-5	2.2454934129041652E-4	0.0	1.890941821392981E-4	1.1818386383706132E-5
14-15	9.454709106964906E-5	2.954596595926533E-4	0.0	2.3045853448226957E-4	1.1818386383706132E-5
16-17	1.5363902298817973E-4	3.1909643236006555E-4	0.0	4.3137110300527385E-4	1.1818386383706132E-5
18-19	1.5363902298817973E-4	3.309148187437717E-4	0.0	6.20465285144572E-4	1.1818386383706132E-5
20-21	1.5363902298817973E-4	3.604607847030371E-4	0.0	8.922881719698129E-4	1.1818386383706132E-5
22-23	1.6545740937188585E-4	4.018251370460085E-4	0.0	0.0014713891047714134	1.1818386383706132E-5
24-25	1.7136660256373892E-4	4.4318948938897994E-4	0.0	0.0020032164920381896	1.1818386383706132E-5
26-27	1.890941821392981E-4	4.6682626215639224E-4	0.0	0.0031555091644495372	1.1818386383706132E-5
28-29	1.890941821392981E-4	4.727354553482453E-4	0.0	0.008391054332431353	1.1818386383706132E-5
30-31	2.068217617148573E-4	5.31827387266776E-4	0.0	0.023489042937615938	1.1818386383706132E-5
32-33	2.304585344822696E-4	5.31827387266776E-4	0.0	0.051380434803162414	3.5455159151118396E-5
34-35	2.718228868252411E-4	5.968285123771597E-4	0.0	0.08851971401395893	3.5455159151118396E-5
36-37	2.954596595926533E-4	6.618296374875434E-4	0.0	0.14032561072693478	3.5455159151118396E-5
38-39	2.954596595926533E-4	6.736480238712496E-4	0.0	0.23928686911089808	4.727354553482453E-5
40-41	2.954596595926533E-4	7.209215694060741E-4	0.0	0.6703743308429629	4.727354553482453E-5
42-43	3.131872391682125E-4	7.327399557897803E-4	0.0	0.7567312801487036	4.727354553482453E-5
44-45	3.1909643236006555E-4	8.391054332431354E-4	0.0	0.8896940361585896	4.727354553482453E-5
46-47	3.1909643236006555E-4	9.100157515453722E-4	0.0	1.0517122950928168	5.3182738726677594E-5
48-49	3.1909643236006555E-4	9.100157515453722E-4	0.0	1.1781276650461296	5.909193191853066E-5
50-51	3.250056255519186E-4	0.0010459271949579928	0.0	1.2974401847828347	5.909193191853066E-5
52-53	3.309148187437717E-4	0.001063654774533552	0.0	1.603359116325068	6.500112511038372E-5
54-55	3.309148187437717E-4	0.001081382354109111	0.0	2.5354988871807382	7.681951149408986E-5
56-57	3.309148187437717E-4	0.0011227467064520826	0.0	3.5565365604146457	8.272870468594292E-5
58-59	3.309148187437717E-4	0.0011227467064520826	0.0	4.5963654679986465	8.272870468594292E-5
60-61	3.309148187437717E-4	0.001140474286027642	0.0	5.380604042455426	8.272870468594292E-5
62-63	3.4273320512747784E-4	0.0011700202519869072	0.0	5.409671363766151	8.272870468594292E-5
64-65	3.4273320512747784E-4	0.0012291121839054378	0.0	5.424674805280266	8.272870468594292E-5
66-67	3.4273320512747784E-4	0.001240930570289144	0.0	5.434619977422155	8.272870468594292E-5
68-69	3.4273320512747784E-4	0.0012763857294402622	0.0	5.442591479037965	8.272870468594292E-5
70-71	3.4273320512747784E-4	0.0013000225022076746	0.0	5.449895241823095	8.272870468594292E-5
72-73	3.6636997789489013E-4	0.0013000225022076746	0.0	5.455225334082146	8.272870468594292E-5
74-75	3.6636997789489013E-4	0.0013177500817832337	0.0	5.460271785067989	8.272870468594292E-5
76-77	3.6636997789489013E-4	0.0013591144341262053	0.0	5.464928229303169	8.272870468594292E-5
78-79	3.6636997789489013E-4	0.0013709328205099114	0.0	5.46914148404896	8.272870468594292E-5
80-81	3.6636997789489013E-4	0.0013768420137017644	0.0	5.470784239756295	8.863789787779598E-5
82-83	3.722791710867432E-4	0.0013886604000854705	0.0	5.471487433746126	9.454709106964906E-5
84-85	3.781883642785962E-4	0.001418206366044736	0.0	5.47245654142959	9.454709106964906E-5
86-87	3.781883642785962E-4	0.0014891166843469727	0.0	5.472787456248334	1.0636547745335519E-4
88-89	3.781883642785962E-4	0.001524571843498091	0.0	5.4729174584985545	1.0636547745335519E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8595	0.0	41.92883	1
TATCTCG	13220	0.0	39.795143	94-95
CCGTCTT	5215	0.0	35.203773	92-93
TGCCGTC	5405	0.0	32.936165	90-91
TATGCCG	5385	0.0	32.32652	88-89
GCCGTCT	5325	0.0	31.625471	90-91
ATGCCGT	5450	0.0	30.166473	88-89
GTATCAA	13705	0.0	29.762571	1
CGTATGC	6410	0.0	27.732277	86-87
TCAACGC	15420	0.0	25.579596	4
CTCGTAT	6855	0.0	25.3656	84-85
CGCTATC	17780	0.0	25.29935	92-93
GCTATCT	20215	0.0	25.27834	94-95
ATCAACG	15715	0.0	25.126175	3
CAACGCA	15965	0.0	24.653847	5
TCGTATG	6870	0.0	24.002523	84-85
AACGCAG	16430	0.0	23.982115	6
ATCTCGT	10160	0.0	23.974934	94-95
TATCAAC	17150	0.0	23.269114	2
TCTCGTA	7055	0.0	22.911875	82-83
>>END_MODULE