FastQCFastQC Report
Fri 27 May 2016
SRR2139744_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139744_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7586179
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73100.045.449781
GTATCAA121800.033.2846261
TCAACGC142450.027.5340084
ATCAACG145000.027.0794833
CAACGCA147950.026.5391925
AACGCAG152650.025.666336
TATCAAC156950.025.234522
GTACATG195400.022.6415881
TACATGG196800.021.746012
ACGCAGA185300.021.189827
ACATGGG202150.020.6022973
CGCAGAG190250.020.5714138
CATGGGG152850.018.8418794
GTGGTAT41200.017.1399271
GAGTACT125600.016.86688612-13
GCAGAGT234650.016.6604739
AGAGTAC200950.016.35320510-11
CAGAGTA222100.016.1175110-11
TATACTG40300.015.4116375
ATGGGGG95000.015.404085
Produced by FastQC (version 0.11.5)