##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139744_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7586179
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.869065968519855	31.0	31.0	34.0	30.0	34.0
2	32.045630349613425	33.0	31.0	34.0	30.0	34.0
3	32.10588018025939	34.0	31.0	34.0	30.0	34.0
4	35.785836453371324	37.0	35.0	37.0	35.0	37.0
5	35.550567420041105	37.0	35.0	37.0	33.0	37.0
6	35.5828523160342	37.0	35.0	37.0	33.0	37.0
7	35.5412329448066	37.0	35.0	37.0	33.0	37.0
8	35.533602357655944	37.0	35.0	37.0	33.0	37.0
9	37.308353124807624	39.0	37.0	39.0	34.0	39.0
10-11	37.163562776464936	39.0	37.0	39.0	33.0	39.0
12-13	37.168448503521994	39.0	37.0	39.0	33.0	39.0
14-15	38.30782301604009	40.0	38.0	41.0	33.5	41.0
16-17	38.23939416668128	40.0	38.0	41.0	33.0	41.0
18-19	38.2996985702552	40.0	38.0	41.0	33.5	41.0
20-21	38.33754416250899	40.0	38.0	41.0	34.0	41.0
22-23	38.32418198042342	40.0	38.0	41.0	34.0	41.0
24-25	38.276753493714004	40.0	38.0	41.0	34.0	41.0
26-27	38.16405736072274	40.0	38.0	41.0	34.0	41.0
28-29	37.99523748713398	40.0	37.0	41.0	33.0	41.0
30-31	37.90563612997551	40.0	37.0	41.0	33.0	41.0
32-33	37.82051664056261	40.0	37.0	41.0	33.0	41.0
34-35	37.75144616121076	40.0	37.0	41.0	33.0	41.0
36-37	37.70894481287782	39.5	37.0	41.0	33.0	41.0
38-39	37.618777596188266	39.0	37.0	41.0	32.5	41.0
40-41	37.60006844327604	39.0	37.0	41.0	32.5	41.0
42-43	37.54604034916545	39.0	37.0	41.0	32.0	41.0
44-45	37.474091582692395	39.0	37.0	41.0	32.0	41.0
46-47	37.415362122950015	39.0	36.0	40.5	32.0	41.0
48-49	37.42166016163689	39.0	36.0	40.5	32.0	41.0
50-51	37.30870981410915	39.0	36.0	40.0	31.5	41.0
52-53	37.11868987721188	39.0	36.0	40.0	31.0	41.0
54-55	36.92088316236941	39.0	35.0	40.0	31.0	41.0
56-57	36.79529760021825	39.0	35.0	40.0	31.0	41.0
58-59	36.59595709287403	38.0	35.0	40.0	30.5	41.0
60-61	36.40964312565079	38.0	35.0	40.0	30.0	41.0
62-63	36.258852777838996	38.0	34.5	40.0	30.0	41.0
64-65	35.99314685701236	37.5	34.0	40.0	30.0	41.0
66-67	35.84157000878929	37.0	34.0	40.0	30.0	41.0
68-69	35.56377764110823	36.5	34.0	39.0	29.0	41.0
70-71	35.23685365587883	36.0	34.0	39.0	29.0	40.5
72-73	34.94263031311117	36.0	34.0	39.0	29.0	40.0
74-75	34.5860644397535	35.0	33.0	38.0	29.0	40.0
76-77	32.45506280057378	33.5	30.5	35.5	26.5	38.5
78-79	33.72615666716966	35.0	32.5	36.0	28.5	39.0
80-81	33.72974986156643	35.0	33.0	36.0	29.0	39.0
82-83	33.56441073627156	35.0	33.0	36.0	29.0	37.5
84-85	33.37089904248263	35.0	33.0	35.5	29.0	37.0
86-87	33.14723532029314	35.0	32.5	35.0	29.0	37.0
88-89	32.99199666381877	34.5	32.0	35.0	29.0	36.0
90-91	32.8416905221938	34.0	32.0	35.0	28.0	36.0
92-93	32.8183826022995	34.0	32.0	35.0	29.0	36.0
94-95	32.786625056792275	34.0	32.0	35.0	29.0	35.0
96-97	32.82041129711086	34.0	32.0	35.0	29.0	35.0
98-99	32.9633029076031	34.0	32.0	35.0	29.0	35.0
100-101	31.746409278601977	33.0	30.5	34.5	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	4.0
19	7.0
20	49.0
21	257.0
22	1210.0
23	4123.0
24	10855.0
25	23595.0
26	43033.0
27	69338.0
28	101385.0
29	137775.0
30	178809.0
31	227087.0
32	290442.0
33	380220.0
34	519325.0
35	741970.0
36	1153067.0
37	1706605.0
38	1537065.0
39	455440.0
40	4517.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.54128184953189	20.29063643238579	13.809376235388065	24.35870548269425
2	15.134146132481252	21.36537338537568	40.18535142424398	23.315129057899085
3	18.602512317855375	25.625555124067017	30.3097938319636	25.462138726114013
4	12.99440099918602	17.664593817042185	38.11554571308993	31.225459470681866
5	13.30969121609179	37.78430485228466	35.631745573100766	13.274258358522781
6	30.901103177238504	37.47658735708714	18.32970194876762	13.292607516906733
7	27.04033572636972	32.33481998445067	22.82125465550637	17.80358963367324
8	24.842735086675145	36.58657924344375	20.970777416809856	17.59990825307125
9	25.700830418053673	16.515376186087884	20.99148991870611	36.79230347715233
10-11	24.507713750585687	26.5332772751117	28.79608861426014	20.162920360042474
12-13	25.04887696187541	24.000155283177264	28.954037959091316	21.99692979585601
14-15	22.164550823280074	25.500894455561884	26.297158292732085	26.037396428425957
16-17	22.01901128479906	28.69159353421489	27.032207322830594	22.25718785815546
18-19	22.085973150403415	28.01151045375798	28.43173692575843	21.47077947008017
20-21	22.747490451932208	27.20183610806889	28.35751746546323	21.693155974535674
22-23	22.490266643410237	27.07301609400344	28.23181651045702	22.2049007521293
24-25	22.299735010180175	27.224164877221074	28.268325913016163	22.207774199582587
26-27	22.277367128199163	27.403213631896396	28.18019985280864	22.139219387095803
28-29	22.252506344451	27.46062948597587	28.1596527702282	22.127211399344933
30-31	22.44703365256022	27.34192446483106	28.06366305101204	22.147378831596686
32-33	22.250098630199414	27.457554569857074	28.05138255825938	22.24096424168413
34-35	22.41131988136304	27.438570404425345	28.03613288359984	22.11397683061178
36-37	22.372474322232954	27.386091731758675	28.022453567098253	22.218980378910114
38-39	22.20471367486876	27.508750192299935	28.088129023748316	22.19840710908299
40-41	22.470695295466182	27.34108582281519	27.969818560642928	22.218400321075695
42-43	22.37886101343695	27.43507865740845	27.793548187192645	22.39251214196195
44-45	22.41103298569437	27.495630400019834	27.676537078404056	22.416799535881744
46-47	22.457855956763296	27.50621965024561	27.521326069997176	22.514598322993912
48-49	22.333054890163687	27.609595949701188	27.543263214970697	22.514085945164428
50-51	22.346955677814215	27.682305969685437	27.484912885783398	22.48582546671695
52-53	22.54061716495887	27.57503006133561	27.314559888727008	22.569792884978515
54-55	22.51250466258917	27.476297984512172	27.371070708945567	22.64012664395309
56-57	22.418559990556325	27.57220867213061	27.42147694918878	22.58775438812429
58-59	22.406269611411986	27.57730798639859	27.38915459332121	22.62726780886821
60-61	22.485490367550003	27.48943062221149	27.45364742443034	22.571431585808167
62-63	22.46496984107093	27.607391626918687	27.380625757577437	22.547012774432947
64-65	22.43394860996398	27.613207628151322	27.28556601108237	22.667277750802327
66-67	22.44123891128538	27.700807518281458	27.17812885519169	22.67982471524147
68-69	22.43575435529624	27.68023481914919	27.221007702962748	22.663003122591824
70-71	22.431144729957694	27.516008994979295	27.238620070163304	22.8142262048997
72-73	22.45711681916249	27.53127883765153	27.179190653074325	22.832413690111654
74-75	22.37856121010957	27.63449687289119	27.147892760246567	22.839049156752672
76-77	22.548829115499824	27.577043695773124	27.086796330941628	22.787330857785424
78-79	22.558772234319292	27.534059772470776	27.024124834557924	22.883043158652004
80-81	22.539174765537435	27.57305171187896	27.05189629347316	22.835877229110444
82-83	22.562507314992875	27.55611497686074	27.105590328335506	22.77578737981088
84-85	22.63206707798099	27.598692160870037	27.08039200153618	22.688848759612792
86-87	22.53808103350243	27.56537507320813	27.143055942109655	22.753487951179782
88-89	22.524509893153493	27.622624879489738	27.111103136326687	22.741762091030086
90-91	22.595588095975636	27.59249704003303	27.161441247244376	22.650473616746957
92-93	22.634000575461908	27.51366452867705	27.219285348806267	22.633049547054775
94-95	22.509807725568535	27.61741130951782	27.233631141667757	22.639149823245887
96-97	22.484985625862677	27.60400963666631	27.29326260741204	22.617742130058975
98-99	22.68804159530772	28.14327841834591	27.41729134989206	21.751388636454312
100-101	23.529886327517715	30.724970399144613	24.353196310477525	21.39194696286015
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1382.0
1	1181.0
2	1430.0
3	1992.0
4	2632.0
5	4215.5
6	5665.5
7	6673.5
8	7353.0
9	7350.5
10	6774.0
11	6120.5
12	6547.5
13	8677.0
14	13698.0
15	20663.0
16	26443.0
17	29921.0
18	31343.5
19	30950.5
20	29567.0
21	28157.0
22	27863.5
23	30199.5
24	35600.0
25	44280.5
26	56445.0
27	70354.5
28	85941.5
29	103068.5
30	119792.0
31	136972.0
32	154782.0
33	171909.5
34	188436.5
35	204664.0
36	222825.5
37	240099.5
38	254199.0
39	267022.0
40	279808.0
41	292125.5
42	300622.5
43	307740.5
44	314754.0
45	320359.0
46	324051.5
47	325586.0
48	324451.5
49	320031.5
50	314828.5
51	307131.0
52	295063.5
53	281136.0
54	266702.5
55	250741.5
56	232805.5
57	212450.0
58	190138.5
59	165383.0
60	138904.5
61	114017.0
62	91495.5
63	71007.0
64	53088.5
65	39703.0
66	30165.0
67	23247.5
68	17945.0
69	13287.0
70	9568.0
71	6898.0
72	4942.0
73	3456.5
74	2361.0
75	1616.5
76	1081.0
77	711.5
78	494.5
79	345.0
80	245.5
81	189.5
82	157.0
83	113.0
84	77.0
85	56.0
86	41.5
87	33.0
88	23.5
89	15.5
90	12.0
91	9.0
92	6.0
93	4.0
94	2.5
95	2.0
96	2.0
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	6.459114661017094E-4
3	4.877290662400663E-4
4	5.272746662054771E-5
5	0.0
6	0.0
7	1.3181866655136927E-5
8	0.0012390954655828713
9	0.0
10-11	2.4386453312003314E-4
12-13	5.075018662227717E-4
14-15	0.0
16-17	6.5909333275684635E-6
18-19	4.1522879963681327E-4
20-21	0.0
22-23	0.0
24-25	1.1209091760262582E-4
26-27	0.0
28-29	5.214544857363056E-4
30-31	2.577583955214016E-4
32-33	0.0010002022263176785
34-35	0.0010362823715348644
36-37	4.002274092139154E-5
38-39	0.0015162364435211653
40-41	4.1848742445289524E-4
42-43	1.6307941777386265E-4
44-45	1.301238847869427E-4
46-47	4.8373690351439005E-5
48-49	2.7888465937062565E-5
50-51	4.9214664029416875E-5
52-53	4.250953028244324E-5
54-55	3.069714355226489E-4
56-57	5.614349066970763E-4
58-59	5.880666466820808E-4
60-61	4.615747450330711E-4
62-63	8.132572164009435E-5
64-65	7.464377192894063E-5
66-67	1.5840322309826747E-4
68-69	1.0679688970841092E-4
70-71	1.080384129033672E-4
72-73	0.0
74-75	7.897237356838881E-6
76-77	8.711951550679421E-4
78-79	2.4268742345234182E-5
80-81	2.3768925392129897E-4
82-83	2.491977079791611E-5
84-85	1.683667074029915E-5
86-87	0.0
88-89	1.7336484875130503E-5
90-91	6.168889023625259E-5
92-93	1.1663542332647457E-4
94-95	4.3010134743431277E-4
96-97	8.442676151982054E-5
98-99	9.851104495984984E-6
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	226.0
20-21	692.0
22-23	1354.0
24-25	3051.0
26-27	4294.0
28-29	7635.0
30-31	15856.0
32-33	20047.0
34-35	27496.0
36-37	41081.0
38-39	45105.0
40-41	48054.0
42-43	54747.0
44-45	64525.0
46-47	65069.0
48-49	60946.0
50-51	56360.0
52-53	51590.0
54-55	57081.0
56-57	58396.0
58-59	62452.0
60-61	61954.0
62-63	62925.0
64-65	68335.0
66-67	73596.0
68-69	75254.0
70-71	74927.0
72-73	72919.0
74-75	76602.0
76-77	73120.0
78-79	79540.0
80-81	81750.0
82-83	79549.0
84-85	81714.0
86-87	86972.0
88-89	92851.0
90-91	99520.0
92-93	105726.0
94-95	123584.0
96-97	188076.0
98-99	677872.0
100-101	4503336.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.1259055281299
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.97583179097401	79.26351725963438
2	6.488957238631618	11.30712550698186
3	1.4560483976113703	3.8057860540401953
4	0.5029919404734275	1.7529451314839433
5	0.23500780416470446	1.0237633872013645
6	0.11247589440976616	0.587973849032232
7	0.0676163350847193	0.41237940959247404
8	0.043396754292054356	0.30247852117415946
9	0.025648936961770043	0.20112181727653225
>10	0.08999675902589666	1.1990324750706562
>50	0.001711693360227434	0.09497943375731333
>100	2.9375248594947265E-4	0.03764245993862012
>500	2.270252443680351E-5	0.011254694816193409
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.6363733310273854E-5
2	0.0	1.3181866655136927E-5	0.0	0.0	2.6363733310273854E-5
3	0.0	1.3181866655136927E-5	0.0	0.0	2.6363733310273854E-5
4	0.0	2.6363733310273854E-5	0.0	1.3181866655136927E-5	2.6363733310273854E-5
5	0.0	2.6363733310273854E-5	0.0	1.3181866655136927E-5	2.6363733310273854E-5
6	0.0	2.6363733310273854E-5	0.0	1.3181866655136927E-5	2.6363733310273854E-5
7	0.0	2.6363733310273854E-5	0.0	1.3181866655136927E-5	2.6363733310273854E-5
8	0.0	2.6363733310273854E-5	0.0	1.3181866655136927E-5	2.6363733310273854E-5
9	0.0	7.909119993082156E-5	0.0	6.590933327568463E-5	3.954559996541078E-5
10-11	0.0	7.909119993082156E-5	0.0	9.886399991352696E-5	3.954559996541078E-5
12-13	0.0	8.568213325839004E-5	0.0	1.0545493324109542E-4	4.613653329297924E-5
14-15	0.0	2.504554664476016E-4	0.0	1.0545493324109542E-4	9.886399991352696E-5
16-17	0.0	2.768191997578755E-4	0.0	1.0545493324109542E-4	1.3181866655136927E-4
18-19	0.0	2.9659199974058087E-4	0.0	1.0545493324109542E-4	1.3181866655136927E-4
20-21	0.0	3.1636479972328626E-4	0.0	1.3181866655136927E-4	1.450005332065062E-4
22-23	0.0	3.7568319967140243E-4	0.0	1.7795519984434852E-4	1.7136426651678008E-4
24-25	0.0	4.086378663092447E-4	0.0	2.768191997578755E-4	1.977279998270539E-4
26-27	0.0	4.2181973296438166E-4	0.0	5.602293328433194E-4	1.977279998270539E-4
28-29	0.0	4.2181973296438166E-4	0.0	0.002135462398132182	1.977279998270539E-4
30-31	0.0	4.2181973296438166E-4	0.0	0.007137980793756646	1.977279998270539E-4
32-33	0.0	4.2181973296438166E-4	0.0	0.01729460905153965	2.1090986648219083E-4
34-35	0.0	4.8772906624006633E-4	0.0	0.03133329703926048	2.2409173313732778E-4
36-37	0.0	5.470474661881825E-4	0.0	0.050539276755794976	2.3068266646489625E-4
38-39	0.0	5.53638399515751E-4	0.0	0.08065984206278286	2.372735997924647E-4
40-41	0.0	6.195477327914356E-4	0.0	0.1352789065483427	2.372735997924647E-4
42-43	0.0	6.854570660671203E-4	0.0	0.16166900359192682	2.4386453312003317E-4
44-45	0.0	7.645482659979419E-4	0.0	0.1979191368935534	2.5045546644760164E-4
46-47	0.0	8.304575992736264E-4	0.0	0.2373131453924301	2.636373331027386E-4
48-49	0.0	8.436394659287634E-4	0.0	0.26802030376557157	3.427285330335601E-4
50-51	0.0	9.425034658422902E-4	0.0	0.299248145871591	3.427285330335601E-4
52-53	0.0	9.622762658249957E-4	0.0	0.3403360242356528	3.493194663611286E-4
54-55	0.0	9.622762658249957E-4	0.0	0.42249859909712123	3.7568319967140243E-4
56-57	0.0	9.622762658249957E-4	0.0	0.5112191526195203	4.0204693298167627E-4
58-59	0.0	9.754581324801326E-4	0.0	0.5957874181455514	4.0863786630924477E-4
60-61	0.0	0.0010018218657904063	0.0	0.642925773304321	4.0863786630924477E-4
62-63	0.0	0.0010281855991006803	0.0	0.682985466069282	4.2181973296438166E-4
64-65	0.0	0.001278641065548282	0.0	0.6998582553878574	4.2181973296438166E-4
66-67	0.0	0.0012918229322034188	0.0	0.7068644175150626	4.2841066629195016E-4
68-69	0.0	0.0013181866655136926	0.0	0.7137651247090268	4.4159253294708705E-4
70-71	0.0	0.001337959465496398	0.0	0.7195783279039422	4.4818346627465555E-4
72-73	0.0	0.0013445503988239666	0.0	0.7247851652327213	4.8772906624006633E-4
74-75	0.0	0.0013577322654791035	0.0	0.7292076814955197	4.8772906624006633E-4
76-77	0.0	0.0013906869321169458	0.0	0.7330633774921473	4.8772906624006633E-4
78-79	0.0	0.0014104597320996513	0.0	0.7364313444225348	4.8772906624006633E-4
80-81	0.0	0.0014170506654272198	0.0	0.7381713508210128	5.075018662227717E-4
82-83	0.0	0.0014434143987374936	0.0	0.7388963534870453	5.272746662054771E-4
84-85	0.0	0.001450005332065062	0.0	0.7395224921531643	5.272746662054771E-4
86-87	0.0	0.0014697781320477674	0.0	0.7398520388195428	5.272746662054771E-4
88-89	0.0	0.001476369065375336	0.0	0.7400431758860422	5.470474661881825E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7310	0.0	45.44978	1
GTATCAA	12180	0.0	33.284626	1
TCAACGC	14245	0.0	27.534008	4
ATCAACG	14500	0.0	27.079483	3
CAACGCA	14795	0.0	26.539192	5
AACGCAG	15265	0.0	25.66633	6
TATCAAC	15695	0.0	25.23452	2
GTACATG	19540	0.0	22.641588	1
TACATGG	19680	0.0	21.74601	2
ACGCAGA	18530	0.0	21.18982	7
ACATGGG	20215	0.0	20.602297	3
CGCAGAG	19025	0.0	20.571413	8
CATGGGG	15285	0.0	18.841879	4
GTGGTAT	4120	0.0	17.139927	1
GAGTACT	12560	0.0	16.866886	12-13
GCAGAGT	23465	0.0	16.660473	9
AGAGTAC	20095	0.0	16.353205	10-11
CAGAGTA	22210	0.0	16.11751	10-11
TATACTG	4030	0.0	15.411637	5
ATGGGGG	9500	0.0	15.40408	5
>>END_MODULE