FastQCFastQC Report
Fri 27 May 2016
SRR2139743_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139743_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5721153
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56100.045.843951
GTATCAA94200.032.653521
TCAACGC106800.028.1611144
ATCAACG109100.027.5285153
CAACGCA111200.027.1236615
TATCAAC116100.026.2735162
AACGCAG115050.026.2531386
GTACATG140550.022.4323061
ACGCAGA137650.022.0358877
TACATGG140400.021.6957472
CGCAGAG141650.021.3533068
ACATGGG144950.020.8378753
CATGGGG107200.018.0132484
GAGTACT100800.017.5043312-13
GCAGAGT173100.017.2760969
GTGGTAT33700.016.8604891
AGAGTAC149000.016.54375610-11
CAGAGTA167150.016.510910-11
TGGTATC32500.016.431562
GAGTACA101600.015.8944861
Produced by FastQC (version 0.11.5)