##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139743_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5721153
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.83699002631113	31.0	31.0	34.0	30.0	34.0
2	32.02384589260242	33.0	31.0	34.0	30.0	34.0
3	32.085352200858814	33.0	31.0	34.0	30.0	34.0
4	35.76772689001675	37.0	35.0	37.0	35.0	37.0
5	35.53286234435611	37.0	35.0	37.0	33.0	37.0
6	35.56284353870627	37.0	35.0	37.0	33.0	37.0
7	35.52349500179422	37.0	35.0	37.0	33.0	37.0
8	35.51361569949274	37.0	35.0	37.0	33.0	37.0
9	37.28197917447759	39.0	37.0	39.0	34.0	39.0
10-11	37.139303563460025	39.0	37.0	39.0	33.0	39.0
12-13	37.14515832735115	39.0	37.0	39.0	33.0	39.0
14-15	38.28289909394138	40.0	38.0	41.0	33.5	41.0
16-17	38.21066688830031	40.0	38.0	41.0	33.0	41.0
18-19	38.2719382788749	40.0	38.0	41.0	33.5	41.0
20-21	38.31341679708956	40.0	38.0	41.0	34.0	41.0
22-23	38.29709508216629	40.0	38.0	41.0	34.0	41.0
24-25	38.25241894709758	40.0	38.0	41.0	34.0	41.0
26-27	38.1375556671977	40.0	38.0	41.0	33.5	41.0
28-29	37.97252632482484	40.0	37.0	41.0	33.0	41.0
30-31	37.878585361685865	40.0	37.0	41.0	33.0	41.0
32-33	37.78922353326968	40.0	37.0	41.0	33.0	41.0
34-35	37.72002266982401	40.0	37.0	41.0	33.0	41.0
36-37	37.675712683866735	39.5	37.0	41.0	33.0	41.0
38-39	37.586101218977795	39.0	37.0	41.0	32.5	41.0
40-41	37.569398578811914	39.0	37.0	41.0	32.0	41.0
42-43	37.518773503055506	39.0	37.0	41.0	32.0	41.0
44-45	37.44889795407725	39.0	36.5	41.0	32.0	41.0
46-47	37.39225635342894	39.0	36.0	40.5	32.0	41.0
48-49	37.402544231826134	39.0	36.0	40.0	32.0	41.0
50-51	37.29114717883766	39.0	36.0	40.0	31.5	41.0
52-53	37.109803749352025	39.0	36.0	40.0	31.0	41.0
54-55	36.91845929557897	39.0	35.0	40.0	31.0	41.0
56-57	36.79481836828435	39.0	35.0	40.0	31.0	41.0
58-59	36.604452063569184	38.0	35.0	40.0	30.5	41.0
60-61	36.426144793568426	38.0	35.0	40.0	30.0	41.0
62-63	36.28615627069125	38.0	34.5	40.0	30.0	41.0
64-65	36.030883860928505	37.5	34.0	40.0	30.0	41.0
66-67	35.89055033702459	37.0	34.0	40.0	30.0	41.0
68-69	35.62201445732967	37.0	34.0	39.0	29.5	41.0
70-71	35.302590134322735	36.0	34.0	39.0	29.0	40.5
72-73	35.00778401467055	36.0	34.0	39.0	29.0	40.0
74-75	34.65505902868403	35.0	33.5	38.5	29.0	40.0
76-77	32.511402998671315	33.5	30.5	35.5	26.5	38.5
78-79	33.77832724709589	35.0	32.5	37.0	28.0	39.0
80-81	33.77809775735357	35.0	33.0	36.0	29.0	39.0
82-83	33.606456434312	35.0	33.0	36.0	29.0	37.5
84-85	33.405036582836296	35.0	33.0	36.0	29.0	37.0
86-87	33.17148269034409	35.0	32.0	35.0	29.0	37.0
88-89	33.00972175746277	34.5	32.0	35.0	28.5	36.0
90-91	32.849688888422854	34.0	32.0	35.0	28.0	36.0
92-93	32.82204286715415	34.0	32.0	35.0	28.5	36.0
94-95	32.78800964471519	34.0	32.0	35.0	29.0	35.5
96-97	32.81775198798104	34.0	32.0	35.0	29.0	35.0
98-99	32.96063405667165	34.0	32.0	35.0	29.0	35.0
100-101	31.743799518140726	33.0	30.5	34.5	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	6.0
20	32.0
21	213.0
22	929.0
23	3232.0
24	8312.0
25	18014.0
26	33069.0
27	53376.0
28	77857.0
29	106026.0
30	137345.0
31	174085.0
32	220421.0
33	289142.0
34	393802.0
35	557673.0
36	860443.0
37	1275063.0
38	1165178.0
39	343521.0
40	3414.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.35626157874121	20.47435193570247	13.76792405307112	24.4014624324852
2	15.087766829076449	21.49912823089864	40.24956630033429	23.16353863969062
3	18.54480248146609	25.642853322435727	30.4674562770248	25.34488791907339
4	12.798128487826485	17.854437980902087	38.25764719639359	31.08978633487784
5	13.059500418884095	37.872296021448825	35.94191590401445	13.126287655652627
6	30.632269404436485	37.811923575544995	18.36234758972536	13.193459430293158
7	26.88669574122559	32.348059910301295	22.924102886253873	17.84114146221924
8	24.632758624607394	36.92201903095226	21.040687797680903	17.404534546759443
9	25.354906607112238	16.589505646851254	21.14620951406124	36.90937823197527
10-11	24.254324293783235	26.732712984561722	28.99904590635095	20.013916815304093
12-13	24.806408386037823	24.1353694467796	29.18901402057304	21.869208146609537
14-15	21.86861634359368	25.754965825944527	26.409825082461523	25.966592748000274
16-17	21.790170617705908	29.009895382976126	27.205136796726116	21.994797202591858
18-19	21.825254010267557	28.236630649870207	28.65078855966665	21.28732678019558
20-21	22.413844639011252	27.443816547066284	28.608860525617054	21.533478288305403
22-23	22.121010312257468	27.420044101361977	28.487282376596124	21.971663209784428
24-25	21.998688887843606	27.527208899615285	28.525039042220158	21.949063170320954
26-27	21.9745722380872	27.729034105584592	28.424734606689427	21.871659049638776
28-29	21.913464628733514	27.787098115852427	28.464700503228237	21.834736752185826
30-31	22.209113334315404	27.58643504392198	28.379818279441082	21.824633342321533
32-33	21.9346707918764	27.71915601166536	28.3771920079872	21.96898118847104
34-35	22.087777812609964	27.6717245348641	28.395894482898786	21.844603169627145
36-37	22.04260093254189	27.743377697148446	28.301346828137515	21.912674542172148
38-39	21.89294480900211	27.755971490118714	28.43469478655373	21.91638891432544
40-41	22.12606915849124	27.580164587791465	28.358885475736802	21.934880777980492
42-43	22.054294825081577	27.662912690143443	28.20802273319839	22.074769751576593
44-45	22.042074671533072	27.79222053491644	28.076629194694476	22.089075598856017
46-47	22.14961197326063	27.73894959375235	27.84336309720362	22.2680753357834
48-49	22.076742896627817	27.81377859084816	27.874417527632556	22.235060984891465
50-51	22.03712748458307	27.921916929722297	27.858521328523317	22.18243425717132
52-53	22.149156149152216	27.871579577084866	27.77344202657227	22.205822247190653
54-55	22.158827994938566	27.767347640728783	27.75055543978161	22.323268924551037
56-57	22.121208577743033	27.904768939844438	27.708242958666702	22.265779523745827
58-59	22.065419005679573	27.906286637932688	27.766856420326853	22.261437936060886
60-61	22.197034892193855	27.934565979194716	27.70238717027616	22.16601195833527
62-63	22.108168876538333	28.021441387130853	27.701223776547906	22.16916595978291
64-65	22.082419677311616	27.969905320563377	27.623668962859938	22.324006039265072
66-67	22.12174704995573	28.06711409796698	27.489678738514804	22.321460113562487
68-69	22.06850838954189	28.0607293801938	27.52434586517591	22.346416365088402
70-71	22.088014228178174	27.870829926558045	27.587299453590695	22.45385639167309
72-73	22.090005645819343	27.892865721489564	27.509293298207904	22.50783533448319
74-75	22.05143881087311	27.966889592978518	27.484709186208477	22.496962409939897
76-77	22.221713139978995	27.909891041804702	27.420012641819834	22.448383176396472
78-79	22.230543424454215	27.889035333546303	27.342142117632257	22.53827912436722
80-81	22.197147927806256	27.946981153327418	27.35340278567438	22.502468133191947
82-83	22.20223844672395	27.958077791258052	27.441479643764932	22.398204118253066
84-85	22.293400012045588	27.956479697563154	27.39138511214197	22.358735178249294
86-87	22.175610061430884	27.918268757880053	27.464925379807394	22.441195800881673
88-89	22.235810676805958	27.95442579745613	27.425678051599313	22.3840854741386
90-91	22.224842081789305	28.009960432185448	27.48298086367286	22.282216622352387
92-93	22.260348885723623	27.9414027016517	27.51357615540994	22.284672257214734
94-95	22.14325535366672	27.971449658093928	27.584176212880273	22.301118775359075
96-97	22.109841047281222	27.947459816622562	27.635992623415788	22.306706512680428
98-99	22.292997635292537	28.52543699740022	27.73856130987522	21.44300405743202
100-101	23.108448154239568	31.144174645468386	24.821791613302757	20.925585586989286
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1187.0
1	993.0
2	1181.0
3	1636.0
4	2134.5
5	3346.0
6	4408.0
7	5170.0
8	5825.5
9	5940.0
10	5527.0
11	5006.0
12	5282.0
13	6946.5
14	11025.5
15	16649.0
16	21197.0
17	23814.5
18	24788.0
19	24658.5
20	23762.5
21	22657.5
22	22578.0
23	24665.0
24	29405.5
25	36716.5
26	46434.0
27	57362.5
28	69960.5
29	83821.0
30	97263.5
31	110917.5
32	124602.0
33	137970.5
34	150383.0
35	162298.0
36	175315.0
37	186957.5
38	196943.5
39	205971.5
40	214555.0
41	222414.0
42	227501.5
43	232002.5
44	236210.0
45	238405.5
46	240381.0
47	241721.0
48	239732.5
49	235840.5
50	232047.5
51	225720.0
52	214755.5
53	202442.0
54	189808.5
55	176359.5
56	162504.0
57	147722.5
58	131992.0
59	114858.0
60	96912.0
61	79631.5
62	63677.5
63	49386.5
64	37348.5
65	28174.5
66	21266.5
67	16221.5
68	12321.0
69	9124.0
70	6706.0
71	4909.0
72	3596.0
73	2535.5
74	1753.0
75	1251.0
76	878.0
77	555.5
78	372.0
79	276.5
80	204.0
81	145.0
82	110.5
83	86.0
84	65.0
85	52.5
86	44.0
87	31.5
88	19.5
89	10.5
90	5.0
91	4.0
92	4.0
93	3.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.894118370894819E-4
3	3.321008894535769E-4
4	6.991597672706884E-5
5	0.0
6	0.0
7	0.0
8	9.089076974518948E-4
9	0.0
10-11	2.884034039991589E-4
12-13	4.282353574532966E-4
14-15	0.0
16-17	0.0
18-19	4.544538487259474E-4
20-21	0.0
22-23	0.0
24-25	1.836043260326716E-4
26-27	0.0
28-29	4.988845554006485E-4
30-31	4.0310909280035764E-4
32-33	0.0012469965604849096
34-35	0.0012680320318980503
36-37	1.768186818131907E-5
38-39	0.00160914477866747
40-41	3.934453788275417E-4
42-43	9.899954658207664E-5
44-45	1.9043364734028126E-4
46-47	5.4882231877429686E-5
48-49	2.7676366956521255E-5
50-51	2.789889255346009E-5
52-53	1.873568769107474E-5
54-55	3.584839036482058E-4
56-57	5.989291147428398E-4
58-59	4.6006871893098435E-4
60-61	5.318741543200946E-4
62-63	1.2685347564862133E-4
64-65	4.924703257080541E-5
66-67	1.592208844461397E-4
68-69	9.052368859086703E-5
70-71	1.6269115575288638E-4
72-73	0.0
74-75	0.0
76-77	7.56611921240482E-4
78-79	3.188199072425362E-5
80-81	2.2591557126302295E-4
82-83	4.357453691433236E-5
84-85	1.1030754515742707E-5
86-87	1.117737930917313E-5
88-89	3.401837264269687E-5
90-91	4.6074695143899914E-5
92-93	7.029871211587759E-5
94-95	3.462690701720241E-4
96-97	4.8922805439139656E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	201.0
20-21	509.0
22-23	1057.0
24-25	2196.0
26-27	3269.0
28-29	5632.0
30-31	11310.0
32-33	14487.0
34-35	19915.0
36-37	29809.0
38-39	32690.0
40-41	35109.0
42-43	39812.0
44-45	46781.0
46-47	47338.0
48-49	44095.0
50-51	40736.0
52-53	36138.0
54-55	40300.0
56-57	41696.0
58-59	46068.0
60-61	46754.0
62-63	46603.0
64-65	50301.0
66-67	54067.0
68-69	53694.0
70-71	52965.0
72-73	51854.0
74-75	55123.0
76-77	51928.0
78-79	56405.0
80-81	58214.0
82-83	56901.0
84-85	58569.0
86-87	62751.0
88-89	67356.0
90-91	72357.0
92-93	77324.0
94-95	91401.0
96-97	141283.0
98-99	515095.0
100-101	3461060.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.85890371976527
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.6175646440271	83.22512824106767
2	5.598536320566082	10.061566724027129
3	1.087700232214033	2.932186513274613
4	0.34457317700699824	1.2385187174834196
5	0.143427731151281	0.6444129342133668
6	0.06575957860884325	0.3545450185718629
7	0.0432860691244951	0.2722747102505576
8	0.027503046913884013	0.19771149157079126
9	0.0171661661410439	0.1388279583455002
>10	0.05305178316599471	0.7444032432660501
>50	7.829928988660633E-4	0.05003382197798905
>100	6.217196082248685E-4	0.12465142714237325
>500	2.653857317982406E-5	0.015739198808689986
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.7478994181767206E-5	0.0	1.7478994181767206E-5	0.0
3	0.0	1.7478994181767206E-5	0.0	3.495798836353441E-5	0.0
4	0.0	6.991597672706882E-5	0.0	3.495798836353441E-5	0.0
5	0.0	1.0487396509060324E-4	0.0	3.495798836353441E-5	0.0
6	1.7478994181767206E-5	1.0487396509060324E-4	0.0	5.243698254530162E-5	1.7478994181767206E-5
7	3.495798836353441E-5	1.0487396509060324E-4	0.0	5.243698254530162E-5	1.7478994181767206E-5
8	3.495798836353441E-5	1.3983195345413765E-4	0.0	5.243698254530162E-5	1.7478994181767206E-5
9	3.495798836353441E-5	2.447059185447409E-4	0.0	6.991597672706882E-5	3.495798836353441E-5
10-11	6.117647963618522E-5	2.447059185447409E-4	0.0	9.613446799971964E-5	3.495798836353441E-5
12-13	6.991597672706882E-5	2.621849127265081E-4	0.0	1.2235295927237044E-4	5.243698254530162E-5
14-15	7.865547381795243E-5	3.146218952718097E-4	0.0	1.3109245636325404E-4	5.243698254530162E-5
16-17	8.739497090883603E-5	3.321008894535769E-4	0.0	1.3983195345413765E-4	5.243698254530162E-5
18-19	8.739497090883603E-5	3.5831938072622773E-4	0.0	2.0100843309032288E-4	5.243698254530162E-5
20-21	8.739497090883603E-5	4.0201686618064576E-4	0.0	2.447059185447409E-4	6.117647963618522E-5
22-23	9.613446799971964E-5	4.369748545441802E-4	0.0	3.4957988363534413E-4	7.865547381795243E-5
24-25	1.0487396509060324E-4	5.156303283621326E-4	0.0	4.894118370894817E-4	1.0487396509060324E-4
26-27	1.0487396509060324E-4	5.768068079983178E-4	0.0	8.215127265430587E-4	1.0487396509060324E-4
28-29	1.0487396509060324E-4	5.855463050892015E-4	0.0	0.002228571758175319	1.0487396509060324E-4
30-31	1.1361346218148683E-4	6.991597672706883E-4	0.0	0.006790589239616561	1.0487396509060324E-4
32-33	1.3109245636325404E-4	6.991597672706883E-4	0.0	0.014830926563229475	1.2235295927237044E-4
34-35	1.3983195345413765E-4	8.389917207248259E-4	0.0	0.02660302914464969	1.4857145054502125E-4
36-37	1.3983195345413765E-4	9.613446799971964E-4	0.0	0.043295468588237365	1.5731094763590486E-4
38-39	1.3983195345413765E-4	0.0010050421654516145	0.0	0.07138421223833727	1.7478994181767207E-4
40-41	1.3983195345413765E-4	0.0010749581421786833	0.0	0.12534186727745264	1.9226893599943927E-4
42-43	1.3983195345413765E-4	0.0011099161305422175	0.0	0.14866758501302096	1.9226893599943927E-4
44-45	1.3983195345413765E-4	0.0012410085869054717	0.0	0.18165918653110658	2.0100843309032288E-4
46-47	1.3983195345413765E-4	0.001345882551996075	0.0	0.22014793171935798	2.272269243629737E-4
48-49	1.3983195345413765E-4	0.001345882551996075	0.0	0.24956507892727217	2.272269243629737E-4
50-51	1.5731094763590486E-4	0.0014857145054502127	0.0	0.28069516756499957	2.272269243629737E-4
52-53	1.7478994181767207E-4	0.0014857145054502127	0.0	0.3227933250517859	2.621849127265081E-4
54-55	1.7478994181767207E-4	0.0015468909850863979	0.0	0.4101358589780766	2.971429010900425E-4
56-57	1.7478994181767207E-4	0.001608067464722583	0.0	0.5059469655854336	2.971429010900425E-4
58-59	1.7478994181767207E-4	0.001651764950177001	0.0	0.6016007612451546	2.971429010900425E-4
60-61	1.7478994181767207E-4	0.0017129414298131863	0.0	0.6494320288235605	2.971429010900425E-4
62-63	1.7478994181767207E-4	0.0017478994181767207	0.0	0.6745668224569418	3.321008894535769E-4
64-65	1.7478994181767207E-4	0.0018877313716308584	0.0	0.6895463204707163	3.321008894535769E-4
66-67	1.7478994181767207E-4	0.001896470868721742	0.0	0.696022287815061	3.321008894535769E-4
68-69	1.7478994181767207E-4	0.0019314288570852762	0.0	0.7027953980604958	3.321008894535769E-4
70-71	1.7478994181767207E-4	0.0019838658396305778	0.0	0.7087557350764784	3.321008894535769E-4
72-73	1.7478994181767207E-4	0.002027563325084996	0.0	0.7132478365811927	4.1949586036241297E-4
74-75	1.7478994181767207E-4	0.0020974793018120647	0.0	0.7172243077575446	4.2823535745329657E-4
76-77	1.7478994181767207E-4	0.002123697793084716	0.0	0.7205278376578987	4.369748545441802E-4
78-79	1.7478994181767207E-4	0.0021324372901755993	0.0	0.72439943486916	4.369748545441802E-4
80-81	1.7478994181767207E-4	0.0021499162843573666	0.0	0.7259375863571557	4.369748545441802E-4
82-83	1.7478994181767207E-4	0.0021761347756300174	0.0	0.7265231326622448	4.369748545441802E-4
84-85	1.7478994181767207E-4	0.002184874272720901	0.0	0.7271261579615158	4.457143516350638E-4
86-87	1.9226893599943927E-4	0.002202353266902668	0.0	0.7274233008626059	4.544538487259474E-4
88-89	1.9226893599943927E-4	0.002272269243629737	0.0	0.7275106958335147	4.544538487259474E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5610	0.0	45.84395	1
GTATCAA	9420	0.0	32.65352	1
TCAACGC	10680	0.0	28.161114	4
ATCAACG	10910	0.0	27.528515	3
CAACGCA	11120	0.0	27.123661	5
TATCAAC	11610	0.0	26.273516	2
AACGCAG	11505	0.0	26.253138	6
GTACATG	14055	0.0	22.432306	1
ACGCAGA	13765	0.0	22.035887	7
TACATGG	14040	0.0	21.695747	2
CGCAGAG	14165	0.0	21.353306	8
ACATGGG	14495	0.0	20.837875	3
CATGGGG	10720	0.0	18.013248	4
GAGTACT	10080	0.0	17.50433	12-13
GCAGAGT	17310	0.0	17.276096	9
GTGGTAT	3370	0.0	16.860489	1
AGAGTAC	14900	0.0	16.543756	10-11
CAGAGTA	16715	0.0	16.5109	10-11
TGGTATC	3250	0.0	16.43156	2
GAGTACA	10160	0.0	15.894486	1
>>END_MODULE