FastQCFastQC Report
Fri 27 May 2016
SRR2139742_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139742_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7188098
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85300.051.293341
GTATCAA156350.035.6209451
TCAACGC195600.028.013364
ATCAACG198100.027.5979083
CAACGCA200050.027.3697765
AACGCAG206750.026.4436346
TATCAAC207450.026.4328842
ACGCAGA240850.022.6827187
GACCGAG97850.022.46566290-91
GTACATG211800.022.1057741
CGCAGAG247150.022.1045238
TACATGG209150.021.9167922
ACCGAGG100500.021.7410692-93
GAGACCG100600.021.72856588-89
AGACCGA97450.021.45992590-91
ACGAGAC101800.021.44624786-87
ACATGGG214500.020.9698163
CGAGACC101550.020.5637988-89
GCCCACG105900.019.98665682-83
CCACGAG108050.019.90854684-85
Produced by FastQC (version 0.11.5)