FastQCFastQC Report
Fri 27 May 2016
SRR2139741_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139741_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13959363
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA129350.045.5730061
GTATCAA218750.033.3413931
TCAACGC243300.029.0278024
ATCAACG247750.028.506823
CAACGCA252250.028.0500955
AACGCAG264200.026.7981416
TATCAAC275500.026.030812
GTACATG335800.022.5369661
ACGCAGA317100.022.3138187
TACATGG337250.021.7942812
CGCAGAG326050.021.7146748
ACATGGG333900.021.3735643
TATCTCG198850.020.94397494-95
CATGGGG249200.018.4449564
GCAGAGT401050.017.5124769
GAGTACT229600.016.90757812-13
AGAGTAC346800.016.88500610-11
CAGAGTA385800.016.51072510-11
GCTATCT291300.016.3213994-95
GTGGTAT81250.015.765231
Produced by FastQC (version 0.11.5)