##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139741_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13959363
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.899705738721746	31.0	31.0	34.0	30.0	34.0
2	32.08455593568274	33.0	31.0	34.0	30.0	34.0
3	32.148445670479376	34.0	31.0	34.0	30.0	34.0
4	35.82016378541055	37.0	35.0	37.0	35.0	37.0
5	35.58662218326151	37.0	35.0	37.0	33.0	37.0
6	35.6139598920094	37.0	35.0	37.0	33.0	37.0
7	35.5786794139532	37.0	35.0	37.0	33.0	37.0
8	35.57266724849838	37.0	35.0	37.0	33.0	37.0
9	37.36147630805217	39.0	37.0	39.0	34.0	39.0
10-11	37.21702533990985	39.0	37.0	39.0	33.5	39.0
12-13	37.226308499893584	39.0	37.0	39.0	33.5	39.0
14-15	38.380251520072946	40.0	38.0	41.0	34.0	41.0
16-17	38.31260197904446	40.0	38.0	41.0	34.0	41.0
18-19	38.372871527160655	40.0	38.0	41.0	34.0	41.0
20-21	38.4145052112185	40.0	38.0	41.0	34.0	41.0
22-23	38.40019158982087	40.0	38.0	41.0	34.0	41.0
24-25	38.356265480934226	40.0	38.0	41.0	34.0	41.0
26-27	38.24834818910563	40.0	38.0	41.0	34.0	41.0
28-29	38.08334820701845	40.0	38.0	41.0	33.5	41.0
30-31	37.99498401475908	40.0	37.0	41.0	33.0	41.0
32-33	37.910376882143275	40.0	37.0	41.0	33.0	41.0
34-35	37.84350612000381	40.0	37.0	41.0	33.0	41.0
36-37	37.80003697820921	40.0	37.0	41.0	33.0	41.0
38-39	37.70745392300054	40.0	37.0	41.0	33.0	41.0
40-41	37.69515416362151	40.0	37.0	41.0	33.0	41.0
42-43	37.64111312287688	40.0	37.0	41.0	33.0	41.0
44-45	37.56882579539735	39.0	37.0	41.0	32.0	41.0
46-47	37.51713542160461	39.0	37.0	41.0	32.0	41.0
48-49	37.5299414924352	39.0	37.0	41.0	32.0	41.0
50-51	37.41489309794165	39.0	36.0	41.0	32.0	41.0
52-53	37.235115503842664	39.0	36.0	40.0	31.0	41.0
54-55	37.06032849516808	39.0	35.5	40.0	31.0	41.0
56-57	36.947558033742844	39.0	35.0	40.0	31.0	41.0
58-59	36.774319345951135	39.0	35.0	40.0	31.0	41.0
60-61	36.59791478839993	38.0	35.0	40.0	31.0	41.0
62-63	36.46173758775585	38.0	35.0	40.0	30.5	41.0
64-65	36.222580850881165	38.0	34.5	40.0	30.0	41.0
66-67	36.08245598546429	37.5	34.0	40.0	30.0	41.0
68-69	35.80648934587808	37.0	34.0	39.5	30.0	41.0
70-71	35.480822501679384	36.5	34.0	39.0	29.0	41.0
72-73	35.18784171092196	36.0	34.0	39.0	29.0	40.0
74-75	34.830613312419764	35.5	33.5	38.5	29.0	40.0
76-77	32.68227539778217	33.5	31.0	36.0	26.5	38.5
78-79	33.914833324181075	35.0	32.5	37.0	28.5	39.0
80-81	33.87897230849322	35.0	33.0	36.5	29.0	39.0
82-83	33.68856240137144	35.0	33.0	36.0	29.0	38.0
84-85	33.490185607070444	35.0	33.0	36.0	29.0	37.0
86-87	33.24812325277574	35.0	33.0	35.0	29.0	37.0
88-89	33.078644139205124	35.0	32.0	35.0	29.0	36.0
90-91	32.92834170849916	34.0	32.0	35.0	29.0	36.0
92-93	32.90560671799796	34.0	32.0	35.0	29.0	36.0
94-95	32.86058889540653	34.0	32.0	35.0	29.0	35.5
96-97	32.8699330251152	34.0	32.0	35.0	29.0	35.0
98-99	33.01268830272644	34.0	32.0	35.0	29.0	35.0
100-101	31.787439186067402	33.0	31.0	34.5	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	6.0
19	18.0
20	78.0
21	406.0
22	2041.0
23	7002.0
24	18929.0
25	41100.0
26	76075.0
27	123040.0
28	180962.0
29	246815.0
30	323420.0
31	412166.0
32	523207.0
33	684250.0
34	929770.0
35	1327751.0
36	2076979.0
37	3182843.0
38	3001833.0
39	793644.0
40	7026.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.3833066738074	20.433718931157532	13.61707550695544	24.565898888079634
2	14.955458298058108	21.471776692175627	40.39452470249536	23.178240307270904
3	18.457896557924425	25.670796049374932	30.535327319186976	25.335980073513664
4	12.631678710679775	17.849634324104922	38.41220898729139	31.10647797792391
5	12.959402230603216	37.911973490480904	36.12217835441345	13.00644592450243
6	30.55089977959596	37.931730839007486	18.375329877158435	13.142039504238124
7	26.762154316221615	32.372668607634075	22.951435746132237	17.913741330012073
8	24.535892731534624	36.916520902781166	21.231080334795234	17.316506030888977
9	25.302006975533196	16.529343065296032	21.106636456119094	37.06201350305168
10-11	24.137715259789257	26.74587513170856	29.16671934949288	19.94969025900931
12-13	24.719025261032847	24.094929071154073	29.27859990752406	21.907445760289022
14-15	21.730846295124376	25.80824252569709	26.465450211836327	25.995460967342204
16-17	21.752572090861165	29.037983323451076	27.242958722400157	21.966485863287602
18-19	21.79647223352093	28.280859646827917	28.65496112488049	21.26770699477066
20-21	22.350326624794974	27.488120626243596	28.622591551602365	21.538961197359065
22-23	22.004925518786337	27.56831428459557	28.47892303614489	21.947837160473206
24-25	21.9175584742485	27.63836696428104	28.53902507839529	21.905049483075167
26-27	21.946360339495552	27.806869892539726	28.47679389146604	21.769975876498684
28-29	21.907443294723173	27.77197949232702	28.480873244386594	21.839703968563214
30-31	22.004409132250338	27.58375918386666	28.551131619578968	21.860700064304034
32-33	21.744152024214262	27.823493365659125	28.462790702109746	21.969563908016866
34-35	22.008102795221166	27.761382545297785	28.44584020406869	21.784674455412365
36-37	21.898727753673132	27.751496236774305	28.504945487550266	21.844830522002294
38-39	21.78906897730711	27.814370766907697	28.525728717247183	21.870831538538003
40-41	21.993761582177903	27.621553772682866	28.485340324832954	21.899344320306273
42-43	22.076431291563363	27.640381183600464	28.344871828433554	21.93831569640262
44-45	21.86098599928867	27.81941741736228	28.23952093680416	22.080075646544895
46-47	22.027132857804407	27.684511796494093	28.028393265362045	22.259962080339456
48-49	21.86821844673824	27.862027609227376	28.11052035307462	22.15923359095977
50-51	21.814574911352718	27.968057073441628	28.100425151108265	22.11694286409739
52-53	21.936916367510435	27.880320557755457	27.941809942082905	22.240953132651207
54-55	21.88400417127703	27.625789191302108	28.024598075391722	22.46560856202914
56-57	21.935363300992144	27.586447255628006	28.1015397852853	22.376649658094546
58-59	21.76529708129483	27.472180645906846	28.310382123062368	22.45214014973596
60-61	21.91006165520585	27.375543557911254	28.3437740900657	22.370620696817202
62-63	21.64615313051116	27.832884168234557	28.353298815898263	22.16766388535602
64-65	21.368391839086033	27.920213805315214	28.20582649256844	22.505567863030315
66-67	21.45044278471324	28.349232139480645	27.82817667393901	22.372148401867104
68-69	21.527679524297987	28.54456708116741	27.51054712783635	22.41720626669825
70-71	21.51979884870868	28.213750686695853	27.49667806982117	22.769772394774296
72-73	21.46121194189706	27.927700832071494	27.464245877393047	23.1468413486384
74-75	21.751839070440013	27.723712291748924	27.48834819528554	23.036100442525523
76-77	22.096311270436257	27.584132369260967	27.393819816455643	22.925736543847137
78-79	22.228413736875556	27.491409064151444	26.910772503062102	23.3694046959109
80-81	22.159888771233277	27.817858113425775	26.87021261319017	23.15204050215078
82-83	22.11671821724827	27.932333979319875	26.93845160020706	23.0124962032248
84-85	22.15211378096663	27.912335125837252	26.905635372269593	23.02991572092652
86-87	22.341494521337253	28.141946504722366	26.875071258395362	22.64148771554502
88-89	22.461984021363957	27.953547404380423	27.138527912075833	22.445940662179787
90-91	22.260761423651967	27.974931796912095	27.31181787359212	22.452488905843822
92-93	22.09879353142949	27.59675624909662	27.299117736740598	23.005332482733294
94-95	21.94730469758278	27.659151809340944	27.773104913362957	22.62043857971332
96-97	21.686852126460643	27.82251379085355	27.94793522747494	22.542698855210865
98-99	21.945014109137155	28.00611064743055	28.278123882198642	21.770751361233657
100-101	22.813352132494856	30.863542302145415	25.411432781035042	20.91167278432469
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2468.0
1	2093.5
2	2556.5
3	3616.5
4	4753.0
5	7560.5
6	10216.5
7	12211.0
8	13616.5
9	13752.5
10	12793.5
11	11510.5
12	12122.5
13	15679.5
14	24540.0
15	36800.5
16	46857.0
17	53118.0
18	55454.5
19	54543.0
20	52420.5
21	50432.0
22	50504.0
23	55420.5
24	65617.0
25	81501.0
26	103398.0
27	129322.5
28	158839.5
29	191831.5
30	225355.0
31	259885.0
32	294164.0
33	326817.5
34	358619.0
35	388798.5
36	421059.0
37	450034.0
38	473671.0
39	495167.0
40	517011.5
41	538049.5
42	552578.0
43	564830.0
44	574119.5
45	578418.0
46	581884.0
47	584124.0
48	587583.5
49	582357.5
50	565719.5
51	545363.0
52	520365.5
53	492075.0
54	458795.0
55	422310.0
56	384697.5
57	345830.0
58	305707.5
59	262247.0
60	217412.0
61	176417.0
62	138894.0
63	106206.0
64	79093.0
65	59007.5
66	45016.5
67	34371.5
68	26346.0
69	19386.0
70	13687.5
71	9845.0
72	7261.0
73	5127.5
74	3580.5
75	2562.0
76	1809.0
77	1206.0
78	804.5
79	568.0
80	413.5
81	312.0
82	240.5
83	179.5
84	134.0
85	98.0
86	71.5
87	51.0
88	33.5
89	26.0
90	24.0
91	21.0
92	15.5
93	8.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	6.089103062940622E-4
3	3.7967348510100355E-4
4	5.01455546359816E-5
5	0.0
6	0.0
7	7.163650662283086E-6
8	0.0010315656953687642
9	0.0
10-11	2.758005504978988E-4
12-13	4.0474626241899435E-4
14-15	7.163650662283086E-6
16-17	0.0
18-19	3.510188824518712E-4
20-21	1.4327924864075453E-5
22-23	0.0
24-25	1.2182904089012598E-4
26-27	0.0
28-29	4.268438128271946E-4
30-31	3.5552757940555865E-4
32-33	0.0011407026080347924
34-35	0.0012736451040955916
36-37	2.1732749982858292E-5
38-39	0.0017550150465231915
40-41	3.837639627949245E-4
42-43	1.6177495955993683E-4
44-45	1.4807084419926546E-4
46-47	4.476406985074988E-5
48-49	4.134590389137123E-5
50-51	3.7855128423523175E-5
52-53	4.947769254462897E-5
54-55	4.269924985424115E-4
56-57	6.263784144498472E-4
58-59	5.586703126154529E-4
60-61	6.029681163133946E-4
62-63	7.746334502293942E-5
64-65	8.205121152130292E-5
66-67	1.8935959256443266E-4
68-69	1.0757789875023168E-4
70-71	1.5288942505690302E-4
72-73	0.0
74-75	8.204412275490873E-6
76-77	7.99713230294082E-4
78-79	1.2556021830401796E-5
80-81	2.030891467002181E-4
82-83	3.4224440802583674E-5
84-85	3.0279016814370598E-5
86-87	2.1883571521420298E-5
88-89	3.10316328929457E-5
90-91	8.541393819708169E-5
92-93	5.4767464420202645E-5
94-95	2.9247227885068424E-4
96-97	6.168808105130537E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	386.0
20-21	1214.0
22-23	2392.0
24-25	5469.0
26-27	7550.0
28-29	13383.0
30-31	26196.0
32-33	34245.0
34-35	47856.0
36-37	67968.0
38-39	53903.0
40-41	78846.0
42-43	87680.0
44-45	102273.0
46-47	102867.0
48-49	96271.0
50-51	88325.0
52-53	21543.0
54-55	23985.0
56-57	23876.0
58-59	26748.0
60-61	109906.0
62-63	111030.0
64-65	117917.0
66-67	128331.0
68-69	121615.0
70-71	120228.0
72-73	118127.0
74-75	124313.0
76-77	116801.0
78-79	128190.0
80-81	130388.0
82-83	127826.0
84-85	132747.0
86-87	142438.0
88-89	153550.0
90-91	164726.0
92-93	178222.0
94-95	213626.0
96-97	337322.0
98-99	1374488.0
100-101	8894596.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.5598216664024
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.63978969156562	74.06725019174638
2	7.846371129202276	13.11282744569101
3	1.953690729295933	4.897501467938155
4	0.7116373445413927	2.3785715840412376
5	0.33000347788993606	1.3787515880887815
6	0.1802191048105826	0.9035445755310579
7	0.1032503055085151	0.6039303980705162
8	0.0634348630219698	0.4240484673238768
9	0.042300896530128716	0.31811898333478456
>10	0.12591931189845437	1.6181489672464684
>50	0.002729464796852065	0.1553914466608361
>100	5.817051078635396E-4	0.08325787058206512
>500	4.31940778347262E-5	0.022702292832455066
>1k	2.8781752619289953E-5	0.03595472091241134
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.4327301324566171E-5	0.0	0.0	7.163650662283086E-6
3	0.0	1.4327301324566171E-5	0.0	0.0	7.163650662283086E-6
4	0.0	2.8654602649132343E-5	0.0	2.1490951986849258E-5	7.163650662283086E-6
5	7.163650662283086E-6	2.8654602649132343E-5	0.0	2.1490951986849258E-5	7.163650662283086E-6
6	6.447285596054776E-5	2.8654602649132343E-5	0.0	2.1490951986849258E-5	7.163650662283086E-6
7	6.447285596054776E-5	2.8654602649132343E-5	0.0	3.581825331141543E-5	7.163650662283086E-6
8	6.447285596054776E-5	2.8654602649132343E-5	0.0	3.581825331141543E-5	7.163650662283086E-6
9	6.447285596054776E-5	1.504366639079448E-4	0.0	7.880015728511395E-5	1.4327301324566171E-5
10-11	6.80546812916893E-5	1.504366639079448E-4	0.0	8.596380794739703E-5	1.4327301324566171E-5
12-13	7.880015728511395E-5	1.504366639079448E-4	0.0	9.670928394082166E-5	1.4327301324566171E-5
14-15	7.880015728511395E-5	2.3998229718648337E-4	0.0	1.1461841059652937E-4	1.7909126655707716E-5
16-17	1.361093625833786E-4	2.543095985110495E-4	0.0	1.1461841059652937E-4	2.1490951986849258E-5
18-19	1.361093625833786E-4	2.793823758290403E-4	0.0	1.4685483857680324E-4	2.1490951986849258E-5
20-21	1.361093625833786E-4	3.008733278158896E-4	0.0	2.6505507450447414E-4	2.1490951986849258E-5
22-23	1.361093625833786E-4	3.5460070778301275E-4	0.0	4.2981903973698515E-4	3.223642798027389E-5
24-25	1.4685483857680324E-4	3.8683713576328664E-4	0.0	6.375649089431947E-4	3.581825331141543E-5
26-27	1.6118213990136944E-4	4.083280877501359E-4	0.0	0.0010136565687130566	4.2981903973698516E-5
28-29	1.6476396523251098E-4	4.083280877501359E-4	0.0	0.003954335165580263	4.2981903973698516E-5
30-31	1.7909126655707715E-4	4.513099917238344E-4	0.0	0.012135224221907548	4.656372930484006E-5
32-33	1.7909126655707715E-4	4.692191183795421E-4	0.0	0.027264854420649423	6.447285596054776E-5
34-35	1.7909126655707715E-4	5.551829263269392E-4	0.0	0.048766551883492104	7.163650662283086E-5
36-37	1.7909126655707715E-4	6.304012582809116E-4	0.0	0.07832735634140325	7.163650662283086E-5
38-39	1.7909126655707715E-4	6.447285596054777E-4	0.0	0.1349345238747642	7.52183319539724E-5
40-41	1.7909126655707715E-4	6.805468129168932E-4	0.0	0.37384585528723624	7.880015728511395E-5
42-43	1.7909126655707715E-4	6.948741142414593E-4	0.0	0.42219691543231597	7.880015728511395E-5
44-45	1.7909126655707715E-4	8.238198261625549E-4	0.0	0.49725406524638693	7.880015728511395E-5
46-47	1.7909126655707715E-4	9.384382367590842E-4	0.0	0.588809102535696	8.238198261625549E-5
48-49	1.7909126655707715E-4	9.456018874213673E-4	0.0	0.6605351547918054	9.312745860968012E-5
50-51	2.077458692062095E-4	0.0010494748220244718	0.0	0.7290375642498874	9.312745860968012E-5
52-53	2.2207317053077566E-4	0.0010566384726867551	0.0	0.9095687245900834	9.670928394082166E-5
54-55	2.2207317053077566E-4	0.0010745475993424627	0.0	1.4729576127506676	1.002911092719632E-4
56-57	2.2207317053077566E-4	0.001088874900667029	0.0	2.093562578750907	1.002911092719632E-4
58-59	2.2207317053077566E-4	0.001096038551329312	0.0	2.7373133000409835	1.002911092719632E-4
60-61	2.2923682119305874E-4	0.0011103658526538784	0.0	3.2229622512144713	1.002911092719632E-4
62-63	2.3640047185534183E-4	0.0011354386299718693	0.0	3.2409286870754777	1.0745475993424629E-4
64-65	2.3640047185534183E-4	0.0013073662458666633	0.0	3.250094578097869	1.0745475993424629E-4
66-67	2.4714594784876643E-4	0.001325275372522371	0.0	3.2562732267940877	1.0745475993424629E-4
68-69	2.5072777317990797E-4	0.0013539299751715033	0.0	3.261570746458846	1.0745475993424629E-4
70-71	2.614732491733326E-4	0.0013718391018272109	0.0	3.266470683511848	1.1461841059652937E-4
72-73	3.152006291404558E-4	0.0013825845778206356	0.0	3.270271000188189	1.2894571192109553E-4
74-75	3.2236427980273887E-4	0.0014040755298074848	0.0	3.273423006479594	1.2894571192109553E-4
76-77	3.2236427980273887E-4	0.001450639259112325	0.0	3.2763207031724875	1.3252753725223707E-4
78-79	3.2236427980273887E-4	0.0014757120364303157	0.0	3.2792004907387247	1.361093625833786E-4
80-81	3.2236427980273887E-4	0.0014757120364303157	0.0	3.2804218931766442	1.504366639079448E-4
82-83	3.2952793046502196E-4	0.0014757120364303157	0.0	3.2808982759456864	1.6118213990136944E-4
84-85	3.2952793046502196E-4	0.0015079484644105897	0.0	3.2815609136319472	1.6476396523251098E-4
86-87	3.2952793046502196E-4	0.001536603067059722	0.0	3.2819190961650615	1.6476396523251098E-4
88-89	3.3669158112730504E-4	0.0016404760016628266	0.0	3.282033714575658	1.755094412259356E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12935	0.0	45.573006	1
GTATCAA	21875	0.0	33.341393	1
TCAACGC	24330	0.0	29.027802	4
ATCAACG	24775	0.0	28.50682	3
CAACGCA	25225	0.0	28.050095	5
AACGCAG	26420	0.0	26.798141	6
TATCAAC	27550	0.0	26.03081	2
GTACATG	33580	0.0	22.536966	1
ACGCAGA	31710	0.0	22.313818	7
TACATGG	33725	0.0	21.794281	2
CGCAGAG	32605	0.0	21.714674	8
ACATGGG	33390	0.0	21.373564	3
TATCTCG	19885	0.0	20.943974	94-95
CATGGGG	24920	0.0	18.444956	4
GCAGAGT	40105	0.0	17.512476	9
GAGTACT	22960	0.0	16.907578	12-13
AGAGTAC	34680	0.0	16.885006	10-11
CAGAGTA	38580	0.0	16.510725	10-11
GCTATCT	29130	0.0	16.32139	94-95
GTGGTAT	8125	0.0	15.76523	1
>>END_MODULE