FastQCFastQC Report
Fri 27 May 2016
SRR2139740_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139740_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20531335
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA202750.046.514341
GTATCAA348500.033.310711
TCAACGC390650.028.7965974
ATCAACG396800.028.3830173
CAACGCA405400.027.770235
AACGCAG421250.026.7974936
TATCAAC434200.026.1178632
ACGCAGA505450.022.3592077
CGCAGAG519550.021.7023988
GTACATG502250.021.561731
TACATGG498300.021.0115172
ACATGGG506450.020.0748083
GAGTACT366500.017.73373812-13
GCAGAGT631700.017.6985369
CATGGGG363050.017.472744
GTGGTAT118400.017.2615641
AGAGTAC546300.016.98171810-11
CAGAGTA611300.016.64937210-11
TGGTATC110250.016.534672
GTACTTT425050.015.28670414-15
Produced by FastQC (version 0.11.5)