##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139740_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20531335
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.307419658780102	31.0	31.0	34.0	30.0	34.0
2	31.51388733367801	31.0	31.0	34.0	30.0	34.0
3	31.564406795758774	31.0	31.0	34.0	30.0	34.0
4	35.351934591686316	37.0	35.0	37.0	33.0	37.0
5	34.98431236936127	37.0	35.0	37.0	32.0	37.0
6	34.99850940038726	36.0	35.0	37.0	32.0	37.0
7	34.96532680412647	36.0	35.0	37.0	32.0	37.0
8	34.93906855058378	36.0	35.0	37.0	32.0	37.0
9	36.65482911851567	38.0	35.0	39.0	32.0	39.0
10-11	36.44844918754674	38.0	35.0	39.0	32.0	39.0
12-13	36.462659685792474	38.0	35.0	39.0	32.0	39.0
14-15	37.4703865822656	39.0	36.5	40.0	32.0	41.0
16-17	37.36300864020777	39.0	36.0	40.0	32.0	41.0
18-19	37.41256925085485	39.0	36.0	40.0	32.0	41.0
20-21	37.455882563442074	39.0	36.0	40.0	32.0	41.0
22-23	37.431153588906696	39.0	36.0	40.0	32.0	41.0
24-25	37.376963821893895	39.0	36.0	40.0	32.0	41.0
26-27	37.22395958614669	39.0	36.0	40.0	31.5	41.0
28-29	37.03162939289098	39.0	36.0	40.0	31.0	41.0
30-31	36.9125053182709	39.0	36.0	40.0	31.0	41.0
32-33	36.795144534999835	38.5	36.0	40.0	30.5	41.0
34-35	36.705334736398065	38.0	35.0	40.0	30.0	41.0
36-37	36.64555365724888	38.0	35.0	40.0	30.0	41.0
38-39	36.55136788261652	38.0	35.0	40.0	30.0	41.0
40-41	36.53078770579161	38.0	35.0	40.0	30.0	41.0
42-43	36.47036001381092	38.0	35.0	40.0	30.0	41.0
44-45	36.39343356375481	38.0	35.0	40.0	30.0	41.0
46-47	36.324665014793155	38.0	35.0	40.0	30.0	41.0
48-49	36.33781169195736	38.0	35.0	40.0	30.0	41.0
50-51	36.21035924502428	38.0	35.0	40.0	29.5	41.0
52-53	36.00908263332304	38.0	34.0	40.0	29.0	41.0
54-55	35.79783975214097	38.0	34.0	40.0	28.5	41.0
56-57	35.665566428502466	38.0	34.0	40.0	28.0	41.0
58-59	35.47012456703617	38.0	34.0	40.0	28.0	41.0
60-61	35.29724823446375	37.0	33.0	40.0	28.0	41.0
62-63	35.17308849303097	37.0	33.0	39.5	28.0	41.0
64-65	34.931836217850545	37.0	33.0	39.0	28.0	40.0
66-67	34.816424530141134	36.0	33.0	39.0	28.0	40.0
68-69	34.56658739166875	36.0	32.5	39.0	27.5	40.0
70-71	34.25626095196529	35.5	32.0	39.0	26.5	40.0
72-73	33.97671849804537	35.0	32.0	38.0	26.0	40.0
74-75	33.63558991525797	35.0	31.5	37.5	26.0	39.5
76-77	31.576075329956524	33.0	29.5	35.5	24.5	38.0
78-79	32.84884967979213	34.0	31.0	36.0	26.0	39.0
80-81	32.884852131261	34.0	31.0	36.0	26.0	38.5
82-83	32.74460010164142	34.0	31.0	36.0	26.0	37.0
84-85	32.54605890961271	34.0	31.0	35.0	26.0	37.0
86-87	32.302102193991914	34.0	31.0	35.0	26.0	36.5
88-89	32.15254724085926	34.0	31.0	35.0	26.0	36.0
90-91	31.99949408211561	34.0	31.0	35.0	26.0	36.0
92-93	31.99189638299567	34.0	31.0	35.0	26.0	35.5
94-95	31.99128519674851	34.0	31.0	35.0	26.0	35.0
96-97	32.07216346650375	34.0	31.0	35.0	26.0	35.0
98-99	32.3048621936821	34.0	31.0	35.0	27.0	35.0
100-101	31.181747408843695	32.5	29.0	34.5	25.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	12.0
19	58.0
20	334.0
21	1736.0
22	7483.0
23	23600.0
24	57441.0
25	115519.0
26	201964.0
27	311283.0
28	439687.0
29	581108.0
30	740314.0
31	923046.0
32	1153153.0
33	1463709.0
34	1907999.0
35	2556678.0
36	3417344.0
37	3838748.0
38	2387120.0
39	401665.0
40	1333.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.67768121264399	21.345173121962112	14.258478564594071	23.718667100799827
2	15.433672997236023	22.201055599272056	39.7998789748869	22.565392428605023
3	18.851438064665043	26.08370951895956	30.727972580803296	24.336879835572102
4	13.14136300843204	18.42761987251388	38.167302629595454	30.263714489458625
5	12.908488415390426	38.13171427966082	36.039195697698176	12.920601607250576
6	30.337949285811177	38.42324914575696	18.22140645018943	13.017395118242433
7	26.58377061209122	32.578227377810556	23.017529059849252	17.820472950248973
8	24.440152642338475	37.29887372990442	21.105475333751688	17.155498294005415
9	25.1534544636284	16.612061514752938	21.16712332636918	37.06736069524948
10-11	24.072577488100787	27.02093474608704	29.08632053088705	19.82016723492512
12-13	24.639030040288105	24.355401349392142	29.302532050602192	21.703036559717557
14-15	21.648395529282325	26.097921204293854	26.52175872932176	25.731924537102056
16-17	21.573564094763643	29.325856274122273	27.31972749554413	21.780852135569955
18-19	21.62967420845202	28.479790594409295	28.823522592893365	21.06701260424532
20-21	22.170169240747853	27.69979937695214	28.869219666553075	21.260811715746932
22-23	21.894444421304208	27.7309894368229	28.71754298621736	21.65702315565553
24-25	21.852856062778635	27.79200277569387	28.784262166624963	21.570878994902536
26-27	21.840646549483985	28.00585455123054	28.680180537268818	21.473318362016656
28-29	21.796455069316057	27.996035716217133	28.70922919054099	21.498280023925815
30-31	21.915297481118934	27.95243244172509	28.685643655809113	21.446626421346867
32-33	21.77867730536544	28.022986939167954	28.635945566600867	21.562390188865734
34-35	21.9223172543117	27.985787178470616	28.688125479716923	21.40377008750076
36-37	21.793064377897686	27.977392783724042	28.618266621899092	21.61127621647918
38-39	21.707385510386555	27.999510660357924	28.69835531761217	21.59474851164336
40-41	21.90073095328315	27.904659931940618	28.609711948458116	21.584897166318118
42-43	21.87311933520401	27.908045107397033	28.51568907109569	21.703146486303265
44-45	21.859789971263318	28.031073409433198	28.337655170698767	21.771481448604714
46-47	21.969078400890762	27.96274693343998	28.100832877388388	21.967341788280873
48-49	21.844665362888186	28.0795553400295	28.175589122697055	21.900190174385266
50-51	21.814846877905126	28.19744943563041	28.129262182825826	21.85844150363864
52-53	21.951692919042337	28.18281526807419	28.01255030728136	21.852941505602118
54-55	21.936310823788943	28.100346488841655	27.99711502524948	21.966227662119923
56-57	21.934588503017434	28.234024815942288	27.927072258601648	21.904314422438627
58-59	21.84157970719506	28.311794558117185	28.007768647586996	21.83885708710076
60-61	21.948141429294076	28.303177777178718	27.924954158797416	21.82372663472979
62-63	21.866639402937103	28.36929989357718	27.91540566813044	21.848655035355275
64-65	21.848408408821236	28.294610910473235	27.891936877590485	21.96504380311505
66-67	21.89774575670799	28.327317734060344	27.782199764273898	21.99273674495777
68-69	21.87724914726199	28.330720328126883	27.82590511250159	21.966125412109538
70-71	21.86410333742583	28.19268814739574	27.865081274980152	22.078127240198278
72-73	21.906789800075146	28.20015962151008	27.810101904606	22.08294867380878
74-75	21.855318662719505	28.276013847522464	27.77077796768105	22.097889522076983
76-77	21.97005940269775	28.241865233621184	27.698724392208774	22.089350971472292
78-79	21.992014388193812	28.2324343258127	27.66257001379423	22.112981272199264
80-81	21.9473963554292	28.250839846244464	27.695469569925034	22.106294228401307
82-83	21.973458848032635	28.258024768303443	27.750207640037267	22.018308743626662
84-85	22.033414966646383	28.293029114296388	27.703464434103747	21.97009148495348
86-87	21.920076994518187	28.298969362417303	27.775118409153343	22.00583523391117
88-89	21.96653595833422	28.310731376582808	27.761967159130535	21.96076550595244
90-91	21.952539788193686	28.3466602257148	27.80025556836019	21.900544417731325
92-93	21.935490560760094	28.32151015193122	27.863780486016026	21.87921880129266
94-95	21.79147918030079	28.43958954963803	27.86766206350858	21.901269206552595
96-97	21.719401562711006	28.466862663366545	27.92726958724196	21.886466186680494
98-99	21.863475802337412	29.09074374617221	28.01133383567308	21.034446615817295
100-101	22.7159839951677	31.773101649576986	25.129123399300518	20.38179095595479
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4675.0
1	3761.5
2	4260.5
3	5992.0
4	7729.5
5	11875.5
6	15757.5
7	19028.0
8	21783.5
9	22283.5
10	20474.0
11	17921.0
12	18603.5
13	24611.5
14	39651.5
15	60878.0
16	78592.5
17	88944.5
18	92683.0
19	91628.5
20	87891.0
21	84073.5
22	83621.0
23	90892.0
24	107555.5
25	133987.0
26	168956.5
27	209479.5
28	255739.0
29	306099.0
30	356297.0
31	407762.5
32	460241.5
33	510907.5
34	558175.5
35	600986.0
36	648049.5
37	690336.5
38	721364.5
39	749529.0
40	776858.5
41	802546.0
42	819010.0
43	830399.5
44	841876.5
45	848590.0
46	852388.0
47	855334.0
48	848074.0
49	832555.0
50	816131.5
51	784773.0
52	737448.0
53	685838.5
54	633323.5
55	582170.5
56	530164.5
57	476998.0
58	422715.5
59	362835.5
60	300273.5
61	243321.0
62	192708.5
63	148237.0
64	111313.5
65	83149.5
66	62778.0
67	48441.5
68	37322.5
69	27783.0
70	20514.0
71	15188.5
72	11143.0
73	8116.0
74	5843.5
75	4107.0
76	2934.0
77	2040.0
78	1396.5
79	1018.0
80	724.5
81	516.0
82	397.0
83	292.5
84	216.5
85	164.0
86	127.5
87	97.5
88	71.0
89	52.0
90	42.0
91	33.5
92	25.0
93	15.0
94	5.5
95	2.5
96	2.0
97	2.0
98	1.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	2.873656291712156E-4
3	1.2663570099070518E-4
4	9.741207768515783E-6
5	0.0
6	0.0
7	0.0
8	9.88732588504352E-4
9	0.0
10-11	1.8264764565967094E-4
12-13	4.4078965152533913E-4
14-15	2.435301942128946E-6
16-17	4.870603884257892E-6
18-19	3.7990710297211556E-4
20-21	0.0
22-23	0.0
24-25	1.7055674106026842E-4
26-27	2.4376899036612764E-6
28-29	4.5858988245829055E-4
30-31	2.6128922054983555E-4
32-33	9.441558708394768E-4
34-35	0.0010050497868571305
36-37	3.4423975866432413E-5
38-39	0.0015479879218427564
40-41	2.9010524646411916E-4
42-43	1.1213889313978361E-4
44-45	1.4288978905556467E-4
46-47	2.776560087902863E-5
48-49	1.5247085500547714E-5
50-51	1.5344814869412043E-5
52-53	1.54351314035183E-5
54-55	2.84625743798225E-4
56-57	4.94884869983847E-4
58-59	3.5934259400290786E-4
60-61	4.228631535548255E-4
62-63	9.321076242355652E-5
64-65	2.953473660921892E-5
66-67	1.1377775466102762E-4
68-69	1.1757552593788561E-4
70-71	7.452945209342051E-5
72-73	0.0
74-75	5.628616245222149E-6
76-77	7.136176674220968E-4
78-79	3.445612542190449E-5
80-81	1.4806832673906685E-4
82-83	0.0
84-85	8.917124865696953E-6
86-87	3.011174045317808E-6
88-89	3.0547647533300374E-5
90-91	8.692793810532114E-5
92-93	7.271064756703376E-5
94-95	2.1317151935481117E-4
96-97	3.6561160905935045E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	790.0
20-21	2548.0
22-23	4917.0
24-25	8968.0
26-27	12428.0
28-29	18947.0
30-31	31719.0
32-33	41176.0
34-35	56763.0
36-37	78567.0
38-39	86494.0
40-41	96618.0
42-43	113191.0
44-45	134834.0
46-47	135195.0
48-49	127427.0
50-51	118643.0
52-53	107984.0
54-55	124601.0
56-57	131467.0
58-59	142788.0
60-61	144934.0
62-63	149154.0
64-65	160544.0
66-67	171957.0
68-69	172139.0
70-71	172704.0
72-73	172438.0
74-75	184849.0
76-77	166804.0
78-79	188724.0
80-81	197938.0
82-83	199562.0
84-85	212370.0
86-87	231677.0
88-89	255078.0
90-91	282464.0
92-93	319734.0
94-95	399145.0
96-97	620051.0
98-99	1985999.0
100-101	1.2567005E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.10086828870678
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.40790673506633	75.19282588294793
2	7.1961495047327215	12.104048433667382
3	1.8665185841091017	4.7092750080174905
4	0.6905166856482575	2.322922113234338
5	0.32699591027749	1.3750319990596485
6	0.16444081971679914	0.8297769432173699
7	0.09905223287799139	0.5831265153681985
8	0.06249896951197768	0.4204974082485389
9	0.043680902548990944	0.3306241648804071
>10	0.1385117399723534	1.8086958896210426
>50	0.0028846903379423244	0.16528233715747492
>100	8.025307006281265E-4	0.11166930973852766
>500	2.0366607292687757E-5	0.013476739367218965
>1k	2.032789205973577E-5	0.03274725547446509
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.870603884257892E-6
2	0.0	1.4611811652773676E-5	0.0	4.870603884257892E-6	4.870603884257892E-6
3	0.0	1.4611811652773676E-5	0.0	9.741207768515783E-6	4.870603884257892E-6
4	0.0	3.896483107406313E-5	0.0	3.409422718980524E-5	4.870603884257892E-6
5	0.0	5.357664272683681E-5	0.0	3.409422718980524E-5	4.870603884257892E-6
6	3.409422718980524E-5	5.357664272683681E-5	0.0	3.409422718980524E-5	9.741207768515783E-6
7	3.409422718980524E-5	5.357664272683681E-5	0.0	3.409422718980524E-5	1.4611811652773676E-5
8	3.409422718980524E-5	5.84472466110947E-5	0.0	3.896483107406313E-5	2.435301942128946E-5
9	3.409422718980524E-5	8.280026603238416E-5	0.0	6.818845437961048E-5	4.3835434958321025E-5
10-11	3.409422718980524E-5	8.280026603238416E-5	0.0	1.3150630487496307E-4	4.3835434958321025E-5
12-13	3.409422718980524E-5	8.280026603238416E-5	0.0	1.5585932429625253E-4	4.3835434958321025E-5
14-15	3.409422718980524E-5	9.741207768515784E-5	0.0	1.704711359490262E-4	5.357664272683681E-5
16-17	5.1141340784707864E-5	1.0228268156941573E-4	0.0	1.9482415537031567E-4	6.331785049535259E-5
18-19	5.357664272683681E-5	1.1445919128006044E-4	0.0	2.3135368450224986E-4	7.062375632173943E-5
20-21	5.357664272683681E-5	1.3150630487496307E-4	0.0	3.0441274276611825E-4	8.280026603238416E-5
22-23	5.357664272683681E-5	1.485534184698657E-4	0.0	4.237425379304366E-4	8.767086991664205E-5
24-25	5.357664272683681E-5	1.6316523012263937E-4	0.0	5.479429369790129E-4	9.254147380089994E-5
26-27	5.357664272683681E-5	1.704711359490262E-4	0.0	8.036496409025521E-4	9.254147380089994E-5
28-29	5.357664272683681E-5	1.753417398332841E-4	0.0	0.001926323836223996	9.254147380089994E-5
30-31	5.357664272683681E-5	1.9969475925457356E-4	0.0	0.005547617824169739	9.254147380089994E-5
32-33	5.6011944668965756E-5	2.070006650809604E-4	0.0	0.013075136127290311	1.0715328545367362E-4
34-35	5.84472466110947E-5	2.824950252869577E-4	0.0	0.02395850050666457	1.0958858739580257E-4
36-37	5.84472466110947E-5	3.458128757823103E-4	0.0	0.039890245812072136	1.1202388933793151E-4
38-39	5.84472466110947E-5	3.506834796665682E-4	0.0	0.0663717191307823	1.2663570099070518E-4
40-41	5.84472466110947E-5	3.8721300879850237E-4	0.0	0.11135418130384606	1.2663570099070518E-4
42-43	5.84472466110947E-5	4.018248204512761E-4	0.0	0.13463566787059877	1.2663570099070518E-4
44-45	6.575315243748153E-5	4.5540146317811286E-4	0.0	0.1685277649992073	1.2663570099070518E-4
46-47	6.818845437961048E-5	4.968015961943049E-4	0.0	0.20600462658662966	1.2663570099070518E-4
48-49	6.818845437961048E-5	5.041075020206918E-4	0.0	0.23501881392515392	1.3150630487496307E-4
50-51	7.062375632173943E-5	5.747312583424312E-4	0.0	0.26318551618781727	1.3150630487496307E-4
52-53	7.305905826386837E-5	5.747312583424312E-4	0.0	0.300781707570404	1.3394160681709201E-4
54-55	7.305905826386837E-5	5.84472466110947E-4	0.0	0.3721896311175089	1.3637690875922096E-4
56-57	7.305905826386837E-5	6.088254855322365E-4	0.0	0.44957865623448257	1.388122107013499E-4
58-59	7.305905826386837E-5	6.136960894164943E-4	0.0	0.5255162413939474	1.4124751264347885E-4
60-61	8.036496409025521E-5	6.404844107799128E-4	0.0	0.5660007008798988	1.5098872041199464E-4
62-63	9.254147380089994E-5	6.575315243748154E-4	0.0	0.5999317628395815	1.5098872041199464E-4
64-65	9.254147380089994E-5	6.964963554488785E-4	0.0	0.614521656774876	1.5098872041199464E-4
66-67	1.0471798351154467E-4	7.038022612752653E-4	0.0	0.6200838864106986	1.5098872041199464E-4
68-69	1.0715328545367362E-4	7.232846768122969E-4	0.0	0.6253831034367712	1.5098872041199464E-4
70-71	1.168944932221894E-4	7.330258845808127E-4	0.0	0.6306530968395382	1.5585932429625253E-4
72-73	1.4611811652773675E-4	7.378964884650705E-4	0.0	0.6347906748392154	1.6560053206476832E-4
74-75	1.4611811652773675E-4	7.67120111770618E-4	0.0	0.6381051207824527	1.6803583400689726E-4
76-77	1.5098872041199464E-4	7.914731311919074E-4	0.0	0.6409130239217274	1.7290643789115515E-4
78-79	1.6072992818051042E-4	8.036496409025521E-4	0.0	0.643492008678442	1.753417398332841E-4
80-81	1.6072992818051042E-4	8.036496409025521E-4	0.0	0.6448923072951662	1.753417398332841E-4
82-83	1.6072992818051042E-4	8.060849428446811E-4	0.0	0.6454012854010711	1.8508294760179989E-4
84-85	1.6072992818051042E-4	8.0852024478681E-4	0.0	0.6459419224322237	1.8508294760179989E-4
86-87	1.6072992818051042E-4	8.255673583817127E-4	0.0	0.6461489230973047	1.8508294760179989E-4
88-89	1.6072992818051042E-4	8.620968875136468E-4	0.0	0.6462024997400315	1.8508294760179989E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	20275	0.0	46.51434	1
GTATCAA	34850	0.0	33.31071	1
TCAACGC	39065	0.0	28.796597	4
ATCAACG	39680	0.0	28.383017	3
CAACGCA	40540	0.0	27.77023	5
AACGCAG	42125	0.0	26.797493	6
TATCAAC	43420	0.0	26.117863	2
ACGCAGA	50545	0.0	22.359207	7
CGCAGAG	51955	0.0	21.702398	8
GTACATG	50225	0.0	21.56173	1
TACATGG	49830	0.0	21.011517	2
ACATGGG	50645	0.0	20.074808	3
GAGTACT	36650	0.0	17.733738	12-13
GCAGAGT	63170	0.0	17.698536	9
CATGGGG	36305	0.0	17.47274	4
GTGGTAT	11840	0.0	17.261564	1
AGAGTAC	54630	0.0	16.981718	10-11
CAGAGTA	61130	0.0	16.649372	10-11
TGGTATC	11025	0.0	16.53467	2
GTACTTT	42505	0.0	15.286704	14-15
>>END_MODULE