FastQCFastQC Report
Fri 27 May 2016
SRR2139739_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139739_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27770809
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA270950.046.7520561
GTATCAA471900.035.1311841
TCAACGC546700.029.5761474
ATCAACG559850.028.8353923
CAACGCA569650.028.3849455
AACGCAG591550.027.3344426
TATCAAC597250.027.3326572
ACGCAGA704500.022.958127
CGCAGAG717750.022.4802938
GTACATG715000.022.307071
TACATGG714500.021.7381342
ACATGGG736150.020.5546383
GTGGTAT153000.019.058651
CATGGGG551100.018.5700234
GCAGAGT872100.018.3680619
GAGTACT494700.018.03600112-13
AGAGTAC757850.017.32632410-11
TGGTATC149500.016.9120272
CAGAGTA839350.016.90378610-11
GAGTACA522750.015.7704011
Produced by FastQC (version 0.11.5)