##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139739_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27770809
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.666181384921124	31.0	31.0	34.0	30.0	34.0
2	31.864336469276065	31.0	31.0	34.0	30.0	34.0
3	31.921447769130527	33.0	31.0	34.0	30.0	34.0
4	35.637861900242086	37.0	35.0	37.0	33.0	37.0
5	35.38119476461777	37.0	35.0	37.0	33.0	37.0
6	35.40684626796432	37.0	35.0	37.0	33.0	37.0
7	35.36490262131002	37.0	35.0	37.0	33.0	37.0
8	35.35088556476695	37.0	35.0	37.0	33.0	37.0
9	37.099413596485434	39.0	37.0	39.0	33.0	39.0
10-11	36.94184035113993	39.0	37.0	39.0	33.0	39.0
12-13	36.94440649892482	39.0	37.0	39.0	33.0	39.0
14-15	38.03905104096896	40.0	37.0	41.0	33.0	41.0
16-17	37.95768169735351	40.0	37.0	41.0	33.0	41.0
18-19	38.015386480098584	40.0	37.0	41.0	33.0	41.0
20-21	38.05548421181839	40.0	37.0	41.0	33.0	41.0
22-23	38.03736711060659	40.0	37.0	41.0	33.0	41.0
24-25	37.988130161628916	40.0	37.0	41.0	33.0	41.0
26-27	37.858099678997114	40.0	37.0	41.0	33.0	41.0
28-29	37.681769446165575	39.0	37.0	41.0	32.5	41.0
30-31	37.58056583415817	39.0	37.0	40.5	32.0	41.0
32-33	37.484066175419635	39.0	36.5	40.0	32.0	41.0
34-35	37.410464668671395	39.0	36.0	40.0	32.0	41.0
36-37	37.35981361745861	39.0	36.0	40.0	31.5	41.0
38-39	37.26466541955644	39.0	36.0	40.0	31.0	41.0
40-41	37.250112765646634	39.0	36.0	40.0	31.0	41.0
42-43	37.19434905058808	39.0	36.0	40.0	31.0	41.0
44-45	37.11794613187301	39.0	36.0	40.0	31.0	41.0
46-47	37.05578113593171	39.0	36.0	40.0	31.0	41.0
48-49	37.065801422616474	39.0	36.0	40.0	31.0	41.0
50-51	36.94450903989829	39.0	35.5	40.0	31.0	41.0
52-53	36.753465269633374	39.0	35.0	40.0	30.5	41.0
54-55	36.552962007387436	38.0	35.0	40.0	30.0	41.0
56-57	36.420611941050296	38.0	35.0	40.0	30.0	41.0
58-59	36.22244693231593	38.0	34.5	40.0	29.5	41.0
60-61	36.03830492298296	38.0	34.0	40.0	29.0	41.0
62-63	35.896679638727434	38.0	34.0	40.0	29.0	41.0
64-65	35.642116846652314	37.0	34.0	40.0	29.0	41.0
66-67	35.49954639065018	37.0	34.0	39.0	29.0	41.0
68-69	35.23101639814072	36.0	34.0	39.0	29.0	40.5
70-71	34.91025438567681	36.0	33.0	39.0	28.5	40.0
72-73	34.61825627478164	35.5	33.0	38.5	28.0	40.0
74-75	34.268411885800056	35.0	33.0	37.5	28.0	39.5
76-77	32.14990735633648	33.5	30.0	35.5	25.5	38.5
78-79	33.42647724240618	35.0	32.0	36.0	27.0	39.0
80-81	33.44511259903796	35.0	32.0	36.0	28.0	39.0
82-83	33.29244806715677	35.0	32.0	36.0	28.0	37.5
84-85	33.10199650890548	34.5	32.0	35.5	27.5	37.0
86-87	32.87132021287975	34.0	32.0	35.0	27.0	36.5
88-89	32.717791241421445	34.0	32.0	35.0	27.0	36.0
90-91	32.56854433618343	34.0	32.0	35.0	27.0	36.0
92-93	32.55148722245515	34.0	32.0	35.0	27.0	36.0
94-95	32.528962945792856	34.0	32.0	35.0	27.0	35.0
96-97	32.57516778142127	34.0	32.0	35.0	27.5	35.0
98-99	32.74564191097036	34.0	32.0	35.0	29.0	35.0
100-101	31.555799351807806	33.0	29.5	34.5	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	8.0
19	29.0
20	206.0
21	1366.0
22	6135.0
23	20729.0
24	52790.0
25	110749.0
26	197833.0
27	311514.0
28	445595.0
29	598410.0
30	768284.0
31	967167.0
32	1223589.0
33	1581524.0
34	2126702.0
35	2977573.0
36	4436158.0
37	6023407.0
38	4753659.0
39	1158792.0
40	8590.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.33973914839859	20.57183137876898	13.762649118360217	24.325780354472208
2	15.395826433070676	21.62595070585591	39.77547616583561	23.202746695237806
3	18.778647510195803	25.61276909529736	30.425716450171397	25.182866944335437
4	13.058011822127591	17.839000345866822	37.801165648601106	31.301822183404475
5	13.184050660396773	37.794079069728085	35.80107598609403	13.220794283781116
6	30.927388539527247	37.62475554817291	18.11669944508999	13.331156467209867
7	26.98755830222873	32.266324407989856	22.694370289838165	18.051746999943248
8	24.85127913876324	36.71006862610451	20.9587683785971	17.47988385653515
9	25.4703923101412	16.390602808870277	20.852766658688264	37.286238222300256
10-11	24.4679370475249	26.606665716745546	28.784887410401605	20.140509825327943
12-13	25.00652981650059	23.943036198040247	29.003947813537284	22.04648617192188
14-15	22.037981393987526	25.5577097360653	26.212582651538263	26.191726218408913
16-17	22.007684796069224	28.831911753667526	26.969186006954025	22.19121744330922
18-19	22.066441932208374	28.029118314822487	28.454663936186993	21.44977581678215
20-21	22.599103293812288	27.21876839767979	28.437857617314865	21.74427069119306
22-23	22.33699808051687	27.257051190442578	28.290262613643367	22.11568811539718
24-25	22.238350695218422	27.386919887655477	28.333823726093353	22.040905691032748
26-27	22.23082452534842	27.58375912000662	28.25747010822305	21.927946246421914
28-29	22.165772958962176	27.616607644660867	28.24017648086148	21.97744291551548
30-31	22.44406277999121	27.432890031747892	28.189867826998498	21.9331793612624
32-33	22.183667053518715	27.56752787071231	28.192838992371005	22.05596608339797
34-35	22.298616517698424	27.472354127136146	28.227477698103108	22.00155165706232
36-37	22.19935970057487	27.590764862023125	28.190792166266316	22.019083271135685
38-39	22.11409565407564	27.547124244692377	28.289930158852773	22.048849942379213
40-41	22.3256651771859	27.386357819286932	28.217257776487454	22.070719227039714
42-43	22.292282251841154	27.465801590501428	28.055665239068905	22.186250918588513
44-45	22.244044326078143	27.562509609858317	27.95253049660211	22.240915567461425
46-47	22.368120417628155	27.4993752414181	27.699718151019216	22.432786189934525
48-49	22.233276914083774	27.629892923381007	27.715642597309596	22.421187565225623
50-51	22.222428022653006	27.713536739710758	27.73892697127508	22.325108266361156
52-53	22.40474560497092	27.617075512678426	27.67261268570967	22.305566196640985
54-55	22.294036068193797	27.543417685946313	27.665389556616294	22.497156689243596
56-57	22.346490572704422	27.67595012159802	27.56824682825792	22.40931247743964
58-59	22.229642523222324	27.782892318335286	27.651400715231368	22.336064443211022
60-61	22.395000518798454	27.77141148292325	27.54713274099569	22.286455257282608
62-63	22.294514186027182	27.891651695611504	27.53060566696265	22.283228451398667
64-65	22.268067884939338	27.842897572539055	27.475408908302835	22.413625634218775
66-67	22.327587820860025	27.86373699886396	27.329098951804365	22.47957622847165
68-69	22.279828138582708	27.86115372085371	27.403250191129935	22.45576794943365
70-71	22.26403396009606	27.682583050539204	27.456041185296638	22.5973418040681
72-73	22.32044592114521	27.70297220235346	27.349389845175313	22.627192031326018
74-75	22.201791993660354	27.81093110778382	27.371486082491188	22.61579081606464
76-77	22.432353025372375	27.7047032494267	27.29410738317703	22.568836342023893
78-79	22.405179346297764	27.711820549536405	27.241540119670855	22.641459984494976
80-81	22.340823742262085	27.798560272584595	27.229461553209028	22.631154431944292
82-83	22.382791888070756	27.758241340761806	27.301765547304214	22.557201223863224
84-85	22.440929241130732	27.781317773681803	27.277319300430296	22.50043368475717
86-87	22.358616168938894	27.801374844489196	27.325390474847065	22.514618511724844
88-89	22.357122233593838	27.84525140236868	27.302114108385723	22.495512255651757
90-91	22.371083821184357	27.871569015484127	27.336084910092996	22.42126225323852
92-93	22.413592366198596	27.811556826719396	27.36518339093229	22.409667416149716
94-95	22.282871003054897	27.86111747267524	27.42412368572809	22.431887838541773
96-97	22.231653557042844	27.81740731067647	27.516137743260188	22.4348013890205
98-99	22.412870002450525	28.422321756997686	27.61470227376938	21.55010596678241
100-101	23.266195836702874	31.069612531687774	24.63451940029662	21.029672231312734
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6553.0
1	5432.0
2	6261.5
3	8730.5
4	11146.5
5	17101.5
6	22800.0
7	27358.5
8	31061.0
9	31567.5
10	28819.5
11	25403.5
12	26540.0
13	34412.5
14	54075.5
15	81180.5
16	102922.5
17	115883.5
18	120836.5
19	118736.0
20	113068.0
21	107245.5
22	105672.0
23	114333.0
24	134638.0
25	166900.0
26	209808.5
27	257672.0
28	314340.5
29	378376.5
30	439649.5
31	503202.5
32	569472.5
33	630314.5
34	687691.5
35	740669.0
36	797539.0
37	851588.0
38	898333.5
39	942636.0
40	984882.0
41	1028808.5
42	1061490.0
43	1088929.0
44	1117740.5
45	1139990.0
46	1159893.0
47	1176420.5
48	1179690.5
49	1171781.0
50	1157504.0
51	1130501.5
52	1084666.0
53	1027372.0
54	966807.0
55	899634.0
56	826235.0
57	747837.5
58	663837.0
59	572590.5
60	478756.0
61	393161.5
62	314549.0
63	244022.5
64	183310.0
65	136104.5
66	102367.0
67	78472.5
68	60356.5
69	44234.0
70	32406.0
71	24064.5
72	17396.0
73	12317.0
74	8647.5
75	5963.5
76	4159.0
77	2862.5
78	1978.0
79	1421.0
80	1043.5
81	782.0
82	615.5
83	446.0
84	302.5
85	208.5
86	154.5
87	126.5
88	93.5
89	63.5
90	40.0
91	26.5
92	20.5
93	14.0
94	11.5
95	10.5
96	9.0
97	8.5
98	4.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.573147292900254E-4
3	2.556641399967859E-4
4	1.4403613520945682E-5
5	1.4403613520945682E-5
6	0.0
7	3.6009033802364205E-6
8	0.0011774954053373093
9	0.0
10-11	2.9527407717938646E-4
12-13	4.3390885731848865E-4
14-15	3.6009033802364205E-6
16-17	1.8004516901182103E-6
18-19	3.906980167556516E-4
20-21	1.800549722234596E-6
22-23	0.0
24-25	1.4950427265198677E-4
26-27	0.0
28-29	4.904826794967799E-4
30-31	3.5385239098060584E-4
32-33	0.0010256056468146615
34-35	0.0011550978539242945
36-37	3.639593875189518E-5
38-39	0.0016293789421918295
40-41	3.290136113114806E-4
42-43	1.164670249315599E-4
44-45	1.6192835150647157E-4
46-47	4.501748619843592E-5
48-49	2.2680593660002935E-5
50-51	2.4746808266126873E-5
52-53	1.916065633601997E-5
54-55	3.2973078985043894E-4
56-57	5.437078909161188E-4
58-59	4.949741246896165E-4
60-61	4.8526159920208786E-4
62-63	1.0543685962401097E-4
64-65	5.017154151760284E-5
66-67	1.4588531679685443E-4
68-69	1.227813914160255E-4
70-71	9.300163713881911E-5
72-73	0.0
74-75	1.0540220448289068E-5
76-77	7.15660073733435E-4
78-79	2.7972638284135987E-5
80-81	1.762464825771431E-4
82-83	1.1005408806255194E-5
84-85	8.907516683667405E-6
86-87	0.0
88-89	2.5142699103034212E-5
90-91	6.496787129938944E-5
92-93	4.717485814048409E-5
94-95	2.8602920627400014E-4
96-97	5.17272594035847E-5
98-99	2.588216981721261E-6
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	976.0
20-21	2901.0
22-23	5651.0
24-25	11829.0
26-27	16107.0
28-29	26884.0
30-31	49554.0
32-33	63888.0
34-35	87397.0
36-37	127035.0
38-39	139636.0
40-41	151460.0
42-43	173957.0
44-45	204371.0
46-47	205415.0
48-49	192533.0
50-51	177542.0
52-53	159529.0
54-55	178256.0
56-57	187107.0
58-59	208101.0
60-61	215602.0
62-63	214791.0
64-65	229764.0
66-67	246994.0
68-69	240490.0
70-71	238694.0
72-73	235047.0
74-75	249081.0
76-77	230204.0
78-79	254707.0
80-81	264055.0
82-83	261642.0
84-85	271918.0
86-87	294510.0
88-89	319428.0
90-91	344996.0
92-93	380029.0
94-95	459614.0
96-97	725021.0
98-99	2594476.0
100-101	1.7129617E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.98946828959157
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.1695229312044	71.40802781545194
2	7.710972093903383	12.490150597622272
3	2.090775899400034	5.07992485215304
4	0.8438757142618684	2.733801816022721
5	0.41832537036627715	1.6939974659005628
6	0.2319563968311144	1.1271615147432876
7	0.13858946796746602	0.785700112485572
8	0.09305264609699133	0.6029027464267893
9	0.06460858060012274	0.4709353130774247
>10	0.2298658405536991	2.9531861384225397
>50	0.006451947631122889	0.3528629296308176
>100	0.0019430902881275144	0.25496670600550797
>500	4.0064010035286066E-5	0.024691791718781907
>1k	1.9956885420426315E-5	0.021690200338689528
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.60090338023642E-6	2.160542028141852E-5
2	0.0	1.440361352094568E-5	0.0	3.60090338023642E-6	2.160542028141852E-5
3	0.0	1.440361352094568E-5	0.0	1.440361352094568E-5	2.160542028141852E-5
4	0.0	3.60090338023642E-5	0.0	2.160542028141852E-5	2.520632366165494E-5
5	0.0	3.960993718260062E-5	0.0	2.520632366165494E-5	2.520632366165494E-5
6	0.0	3.960993718260062E-5	0.0	2.520632366165494E-5	3.960993718260062E-5
7	0.0	3.960993718260062E-5	0.0	3.60090338023642E-5	4.321084056283704E-5
8	0.0	4.321084056283704E-5	0.0	3.960993718260062E-5	5.40135507035463E-5
9	0.0	1.0082529464661976E-4	0.0	6.481626084425556E-5	6.481626084425556E-5
10-11	7.20180676047284E-6	1.0082529464661976E-4	0.0	7.021761591461019E-5	6.841716422449199E-5
12-13	7.20180676047284E-6	1.0262574633673798E-4	0.0	1.062266497169744E-4	7.20180676047284E-5
14-15	7.20180676047284E-6	1.4043523182922038E-4	0.0	1.1162800478732903E-4	1.2063026323792007E-4
16-17	1.080271014070926E-5	1.440361352094568E-4	0.0	1.2603161830827472E-4	1.4763703858969323E-4
18-19	1.440361352094568E-5	1.6384110380075713E-4	0.0	1.5303839366004784E-4	1.5843974873040249E-4
20-21	2.160542028141852E-5	1.8724697577229385E-4	0.0	2.14253751124067E-4	1.6384110380075713E-4
22-23	2.340587197153673E-5	2.0525149267347595E-4	0.0	3.222808525311596E-4	1.8544652408217563E-4
24-25	3.420858211224599E-5	2.322582680252491E-4	0.0	4.1770479210742476E-4	2.160542028141852E-4
26-27	3.960993718260062E-5	2.4126052647584014E-4	0.0	6.481626084425556E-4	2.1965510619442163E-4
28-29	3.960993718260062E-5	2.466618815461948E-4	0.0	0.0019732950523695583	2.2325600957465803E-4
30-31	4.321084056283704E-5	2.862718187287954E-4	0.0	0.005874873864855719	2.2685691295489446E-4
32-33	5.041264732330988E-5	2.9527407717938646E-4	0.0	0.013330544313635229	2.4126052647584014E-4
34-35	5.40135507035463E-5	3.6729214478411484E-4	0.0	0.023470688232380986	2.4846233323631297E-4
36-37	5.761445408378272E-5	4.28507502248134E-4	0.0	0.038463049455995324	2.5026278492643115E-4
38-39	5.761445408378272E-5	4.4831247083943436E-4	0.0	0.06335249362018947	2.736686568979679E-4
40-41	5.761445408378272E-5	5.041264732330989E-4	0.0	0.1120205032557748	2.7726956027820433E-4
42-43	5.761445408378272E-5	5.185300867540445E-4	0.0	0.1337915650926842	2.844713670386772E-4
44-45	6.661671253437378E-5	5.851467992884184E-4	0.0	0.16532647644510465	2.9527407717938646E-4
46-47	7.741942267508304E-5	6.481626084425556E-4	0.0	0.20137872108803168	3.078772390102139E-4
48-49	7.921987436520124E-5	6.589653185832649E-4	0.0	0.22910927801923237	3.2768220760151425E-4
50-51	7.921987436520124E-5	7.255820311176386E-4	0.0	0.2558117770353755	3.312831109817507E-4
52-53	7.921987436520124E-5	7.345842895682297E-4	0.0	0.2945773023753107	3.5468898295328737E-4
54-55	7.921987436520124E-5	7.45386999708939E-4	0.0	0.37610535580724347	3.798953066149423E-4
56-57	7.921987436520124E-5	7.633915166101211E-4	0.0	0.4644967310819069	3.8709711337541516E-4
58-59	7.921987436520124E-5	7.70593323370594E-4	0.0	0.5511398677654655	3.960993718260062E-4
60-61	7.921987436520124E-5	7.759946784409485E-4	0.0	0.5937961692077461	4.0330117858647905E-4
62-63	7.921987436520124E-5	7.939991953421306E-4	0.0	0.6165196699887281	4.0330117858647905E-4
64-65	7.921987436520124E-5	8.588154561863862E-4	0.0	0.6304857737489751	4.0510163027659724E-4
66-67	7.921987436520124E-5	8.786204247776865E-4	0.0	0.6359771514038356	4.105029853469519E-4
68-69	8.282077774543767E-5	8.984253933689869E-4	0.0	0.6409698039405334	4.1950524379754295E-4
70-71	8.282077774543767E-5	9.038267484393415E-4	0.0	0.6459714587356817	4.411106640789615E-4
72-73	8.462122943555587E-5	9.09228103509696E-4	0.0	0.6498766384515482	4.861219563319167E-4
74-75	8.642168112567408E-5	9.272326204108781E-4	0.0	0.6530652383947475	4.8792240802203495E-4
76-77	8.642168112567408E-5	9.560398474527695E-4	0.0	0.6558415349009098	4.969246664726259E-4
78-79	8.642168112567408E-5	9.614412025231242E-4	0.0	0.6586142305036918	5.041264732330988E-4
80-81	8.82221328157923E-5	9.614412025231242E-4	0.0	0.6598943516553659	5.185300867540445E-4
82-83	9.362348788614694E-5	9.668425575934789E-4	0.0	0.6604182830971903	5.437364104156994E-4
84-85	9.722439126638335E-5	9.758448160440699E-4	0.0	0.6609296113771839	5.491377654860541E-4
86-87	9.902484295650155E-5	9.938493329452519E-4	0.0	0.6612482913263348	5.509382171761723E-4
88-89	1.0082529464661976E-4	0.001055064690409271	0.0	0.6613707220412628	5.581400239366451E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	27095	0.0	46.752056	1
GTATCAA	47190	0.0	35.131184	1
TCAACGC	54670	0.0	29.576147	4
ATCAACG	55985	0.0	28.835392	3
CAACGCA	56965	0.0	28.384945	5
AACGCAG	59155	0.0	27.334442	6
TATCAAC	59725	0.0	27.332657	2
ACGCAGA	70450	0.0	22.95812	7
CGCAGAG	71775	0.0	22.480293	8
GTACATG	71500	0.0	22.30707	1
TACATGG	71450	0.0	21.738134	2
ACATGGG	73615	0.0	20.554638	3
GTGGTAT	15300	0.0	19.05865	1
CATGGGG	55110	0.0	18.570023	4
GCAGAGT	87210	0.0	18.368061	9
GAGTACT	49470	0.0	18.036001	12-13
AGAGTAC	75785	0.0	17.326324	10-11
TGGTATC	14950	0.0	16.912027	2
CAGAGTA	83935	0.0	16.903786	10-11
GAGTACA	52275	0.0	15.770401	1
>>END_MODULE