FastQCFastQC Report
Fri 27 May 2016
SRR2139738_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139738_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10638350
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112050.046.633311
GTATCAA196900.033.1368451
GACCGAG160200.027.8067690-91
ACCGAGG163700.027.56127792-93
AGACCGA157950.027.176590-91
TCAACGC234550.027.074944
ATCAACG237500.026.7387683
GAGACCG165050.026.71027488-89
CAACGCA243500.026.0797825
ACGAGAC166400.026.05634986-87
CGAGACC167700.025.47432188-89
TATCAAC253550.025.3744072
AACGCAG251100.025.3386
CACGAGA169200.024.50760886-87
CCACGAG176250.024.22866684-85
GCCCACG177250.023.79016382-83
CCCACGA172100.023.68590784-85
ACGCAGA296100.021.4471047
CCGAGCC192600.021.39755878-79
CGAGCCC189600.021.0861180-81
Produced by FastQC (version 0.11.5)