##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139738_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10638350
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.70431815084106	31.0	31.0	34.0	30.0	34.0
2	31.902129841563777	33.0	31.0	34.0	30.0	34.0
3	31.96486222017512	33.0	31.0	34.0	30.0	34.0
4	35.68282835214107	37.0	35.0	37.0	33.0	37.0
5	35.429285462501234	37.0	35.0	37.0	33.0	37.0
6	35.45658574872983	37.0	35.0	37.0	33.0	37.0
7	35.41333750064625	37.0	35.0	37.0	33.0	37.0
8	35.405646552331895	37.0	35.0	37.0	33.0	37.0
9	37.179300925425466	39.0	37.0	39.0	33.0	39.0
10-11	37.018321779223285	39.0	37.0	39.0	33.0	39.0
12-13	37.0229567555119	39.0	37.0	39.0	33.0	39.0
14-15	38.10425615814482	40.0	37.5	41.0	33.0	41.0
16-17	38.03111304854606	40.0	37.0	41.0	33.0	41.0
18-19	38.09950081544601	40.0	37.0	41.0	33.0	41.0
20-21	38.14509267743341	40.0	37.0	41.0	33.0	41.0
22-23	38.12645171123596	40.0	37.0	41.0	33.0	41.0
24-25	38.07789680858009	40.0	37.0	41.0	33.0	41.0
26-27	37.95109517176343	40.0	37.0	41.0	33.0	41.0
28-29	37.77490396557049	39.5	37.0	41.0	33.0	41.0
30-31	37.67256179360035	39.0	37.0	41.0	33.0	41.0
32-33	37.57241659996149	39.0	37.0	41.0	32.0	41.0
34-35	37.50243875424958	39.0	36.5	40.0	32.0	41.0
36-37	37.46152733800473	39.0	36.0	40.0	32.0	41.0
38-39	37.37810286205427	39.0	36.0	40.0	32.0	41.0
40-41	37.36941168768462	39.0	36.0	40.0	32.0	41.0
42-43	37.31913076630594	39.0	36.0	40.0	31.5	41.0
44-45	37.25267753389927	39.0	36.0	40.0	31.0	41.0
46-47	37.206472835138584	39.0	36.0	40.0	31.0	41.0
48-49	37.22636638511523	39.0	36.0	40.0	31.0	41.0
50-51	37.12573566112038	39.0	36.0	40.0	31.0	41.0
52-53	36.942098576933674	39.0	35.0	40.0	31.0	41.0
54-55	36.75068487000188	39.0	35.0	40.0	30.5	41.0
56-57	36.634260948127036	38.5	35.0	40.0	30.0	41.0
58-59	36.44633041070562	38.0	35.0	40.0	30.0	41.0
60-61	36.27815305901093	38.0	34.5	40.0	30.0	41.0
62-63	36.13942712825934	38.0	34.0	40.0	29.5	41.0
64-65	35.9007239261105	37.5	34.0	40.0	29.0	41.0
66-67	35.77465052955846	37.0	34.0	40.0	29.0	41.0
68-69	35.509246868304075	37.0	34.0	39.0	29.0	41.0
70-71	35.19476307000629	36.0	34.0	39.0	29.0	40.5
72-73	34.89610078431726	36.0	33.5	39.0	29.0	40.0
74-75	34.551269802569735	35.0	33.0	38.0	28.5	40.0
76-77	32.38997961174794	33.5	30.0	35.5	25.5	38.5
78-79	33.686497410897175	35.0	32.0	37.0	28.0	39.0
80-81	33.682013028713584	35.0	33.0	36.0	28.0	39.0
82-83	33.497884247532745	35.0	33.0	36.0	28.0	37.5
84-85	33.312796832064905	35.0	32.5	36.0	28.5	37.0
86-87	33.07895107786223	35.0	32.0	35.0	28.0	37.0
88-89	32.92810715752112	34.0	32.0	35.0	28.0	36.0
90-91	32.765096226862774	34.0	32.0	35.0	27.0	36.0
92-93	32.74054625981773	34.0	32.0	35.0	27.5	36.0
94-95	32.706470786594316	34.0	32.0	35.0	28.0	35.5
96-97	32.73393676852338	34.0	32.0	35.0	29.0	35.0
98-99	32.90921691421718	34.0	32.0	35.0	29.0	35.0
100-101	31.748644528386695	33.0	30.5	34.5	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	5.0
19	18.0
20	83.0
21	465.0
22	1953.0
23	6620.0
24	16756.0
25	35121.0
26	63041.0
27	100720.0
28	146269.0
29	199206.0
30	259331.0
31	329993.0
32	421684.0
33	547926.0
34	737631.0
35	1033718.0
36	1555260.0
37	2245338.0
38	2146959.0
39	782160.0
40	8091.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.65097501022245	21.410068290665375	13.719796772995812	24.21915992611636
2	14.993078304227305	22.946528337404633	39.72425453002828	22.336138828339784
3	17.4976666913886	27.471471783110257	31.578677737580353	23.452183787920788
4	12.654763191660361	18.90129578365066	39.294317257845435	29.14962376684354
5	12.616524179031524	38.31659984866074	36.39025788773635	12.676618084571386
6	28.819779383081023	38.964783072562945	19.24399930440341	12.971438239952624
7	25.081925298566038	33.2251617967072	24.723298255838547	16.96961464888822
8	24.008977434119906	36.246578260347654	22.506951110001484	17.23749319553095
9	25.60102835496106	16.094206338388943	21.874849013239835	36.429916293410166
10-11	23.487663388782124	26.719162137949574	30.283152467432693	19.510022005835605
12-13	24.400924470319904	24.751201036856614	29.82792940381121	21.019945089012275
14-15	21.72379645339738	26.060897601601752	27.305578402665827	24.90972754233504
16-17	21.30824328960788	28.915080816104	28.05458083255392	21.722095061734198
18-19	21.328406485616604	28.349366581722098	29.476138597202002	20.8460883354593
20-21	22.097084326672466	27.73125548431073	29.17671873932896	20.99494144968784
22-23	21.84377254666437	27.625543493896455	29.044155876390548	21.486528083048633
24-25	21.624028124321086	27.762868447510016	29.096389564733244	21.516713863435655
26-27	21.531350744608208	27.9509543858926	29.04271487874077	21.474979990758413
28-29	21.547388761395048	28.024071686763524	28.954839243107998	21.473700308733427
30-31	21.80178619335553	27.87472187157839	28.869647593749765	21.453844341316312
32-33	21.54423592798106	28.059080434864274	28.85357516945894	21.543108467695728
34-35	21.645620877417855	28.083199749410166	28.850497738151436	21.42068163502054
36-37	21.690973627939854	28.000735194655217	28.79288871497325	21.51540246243168
38-39	21.51201043916373	28.090734271681168	28.833046119812707	21.564209169342398
40-41	21.726240640024567	27.948772040527896	28.72267594947569	21.602311369971844
42-43	21.68826702931758	28.04253047119023	28.49519304148404	21.774009458008152
44-45	21.682791808301314	28.134226758408083	28.339483644170233	21.843497789120374
46-47	21.754496402324605	28.079494101050635	28.15286322560091	22.013146271023853
48-49	21.700615819911683	28.204498833085744	28.118951508322347	21.975933838680223
50-51	21.7151571156982	28.310974574798703	28.005285895398234	21.968582414104862
52-53	21.850768187379995	28.15483655221747	27.831113876127905	22.16328138427463
54-55	21.829613199840004	28.042804857188745	27.90020143257384	22.227380510397413
56-57	21.788664972049418	28.12894000719196	27.915903514607905	22.166491506150717
58-59	21.78765322364942	28.11433445424066	27.901018631567275	22.196993690542648
60-61	21.75982327433325	27.822465888056946	28.052374567152043	22.365336270457753
62-63	21.99617152326694	27.95662756589628	28.01677683859684	22.03042407223994
64-65	21.570343852254613	27.959000485272405	27.986201511929586	22.48445415054339
66-67	21.70358480687863	28.081101544067856	27.737425254015708	22.47788839503781
68-69	21.686902300479417	28.469026385625533	27.733871898961297	22.110199414933753
70-71	21.683078949258345	28.210442049412855	27.789444085221405	22.3170349161074
72-73	21.73112097778329	28.129229753891604	27.33379333957257	22.805855928752532
74-75	21.699748198920148	28.155285972241405	27.313853598439497	22.831112230398958
76-77	21.904475620978186	27.676285582545056	27.638852255109676	22.780386541367083
78-79	21.945909829497193	27.64869200978573	27.523649595943784	22.88174856477329
80-81	22.398333310489452	27.643573788017928	27.092216176208556	22.865876725284064
82-83	22.409096247168485	28.04101510156471	27.177452296446386	22.37243635482042
84-85	22.009767970636567	27.580259691354332	27.165565900465477	23.244406437543617
86-87	21.953929556341194	28.060222718035348	27.213536390799952	22.772311334823502
88-89	22.482240028935742	27.53615171383796	27.236105064533533	22.745503192692766
90-91	22.589516525289653	27.900921919928408	27.26903740559904	22.240524149182907
92-93	22.11252575999264	27.776203359093586	27.31091669184511	22.80035418906866
94-95	22.576226691651215	27.252871618391193	27.467280713440413	22.703620976517183
96-97	22.450304680757018	27.752051226885243	27.568489337638518	22.229154754719225
98-99	22.699783926937243	27.954883011819454	27.856987559356906	21.488345501886403
100-101	26.41266572731303	35.18129805200268	28.578862224176284	9.827173996508009
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3678.0
1	3127.5
2	3642.5
3	4942.5
4	6418.5
5	10036.0
6	13292.5
7	15675.5
8	17202.0
9	17043.0
10	15877.5
11	14677.0
12	15957.0
13	20748.0
14	30749.5
15	44073.5
16	55037.5
17	61579.0
18	64041.5
19	63601.0
20	62073.0
21	61310.5
22	63168.5
23	69709.5
24	82317.5
25	100613.0
26	123871.0
27	149668.0
28	177881.5
29	207178.0
30	233710.5
31	259102.5
32	284683.0
33	308696.5
34	330512.5
35	350544.5
36	372715.0
37	392663.5
38	408465.0
39	423022.5
40	436986.0
41	449452.0
42	456922.5
43	463435.0
44	468514.5
45	470882.0
46	470467.0
47	467088.0
48	461720.0
49	452649.0
50	440629.0
51	424835.0
52	404322.5
53	382580.5
54	359537.5
55	334056.5
56	307308.5
57	278735.0
58	248263.0
59	215662.0
60	182097.0
61	149163.0
62	118701.5
63	92615.5
64	70599.5
65	53725.5
66	41273.0
67	31910.0
68	24546.5
69	18089.5
70	13106.5
71	9501.5
72	6716.5
73	4745.0
74	3343.5
75	2344.0
76	1652.5
77	1169.5
78	823.0
79	565.5
80	377.5
81	261.0
82	200.5
83	141.5
84	96.5
85	72.0
86	53.5
87	37.0
88	23.5
89	16.0
90	11.0
91	7.5
92	5.0
93	2.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	5.733971903537673E-4
3	2.1619894062519093E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.001137394426767309
9	0.0
10-11	3.3369836487801214E-4
12-13	3.947980654894791E-4
14-15	0.0
16-17	0.0
18-19	3.759981576090277E-4
20-21	0.0
22-23	0.0
24-25	1.927994484619095E-4
26-27	0.0
28-29	4.6614203244141366E-4
30-31	3.963305453366757E-4
32-33	9.09539520510661E-4
34-35	0.001176531334125563
36-37	4.800171730943847E-5
38-39	0.0016013112507012408
40-41	3.484869701948911E-4
42-43	1.3418980223106947E-4
44-45	1.2606352229952773E-4
46-47	5.637861788638304E-5
48-49	1.0416086186863546E-5
50-51	2.1157117674883544E-5
52-53	5.365954329074876E-6
54-55	3.53637397296219E-4
56-57	5.742934754518254E-4
58-59	4.711164928069205E-4
60-61	5.887801488696638E-4
62-63	1.0365589146398917E-4
64-65	5.271514015140022E-5
66-67	1.4912341083654775E-4
68-69	1.0337063675582566E-4
70-71	1.054307830474495E-4
72-73	0.0
74-75	6.447510206892221E-6
76-77	8.221977609252818E-4
78-79	3.3548608041413475E-5
80-81	2.3300703667544466E-4
82-83	7.002844275230827E-6
84-85	2.8614857749819157E-5
86-87	7.313836352180236E-6
88-89	5.2398100104773736E-5
90-91	1.150902411071497E-4
92-93	7.87646303331728E-5
94-95	3.24136815556752E-4
96-97	8.371499698081863E-6
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	376.0
20-21	1238.0
22-23	2414.0
24-25	5645.0
26-27	7352.0
28-29	15085.0
30-31	39295.0
32-33	53835.0
34-35	71562.0
36-37	106271.0
38-39	117956.0
40-41	124125.0
42-43	138522.0
44-45	158827.0
46-47	157625.0
48-49	149144.0
50-51	140626.0
52-53	124762.0
54-55	134810.0
56-57	138201.0
58-59	80440.0
60-61	151343.0
62-63	145321.0
64-65	151648.0
66-67	159304.0
68-69	160563.0
70-71	157895.0
72-73	151039.0
74-75	155183.0
76-77	147529.0
78-79	155144.0
80-81	157538.0
82-83	150589.0
84-85	152242.0
86-87	155568.0
88-89	161271.0
90-91	167647.0
92-93	173844.0
94-95	189331.0
96-97	251498.0
98-99	849475.0
100-101	4926267.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.66134863414163
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.22343981243584	81.76654549450153
2	5.603976170543434	9.937121699879468
3	1.2501059641149306	3.3250824214204084
4	0.42553956704477863	1.5091564764551524
5	0.1913287878723427	0.8481734182648754
6	0.1014592040045878	0.5397305915035956
7	0.06064973833122217	0.37641013163277665
8	0.036793628014933084	0.26097381447575224
9	0.023968840705323857	0.19125987679178313
>10	0.08094583001744414	1.0923034143627324
>50	0.0014573414132053538	0.08465055238726898
>100	2.592288088624338E-4	0.030067804636678366
>500	7.588669311636076E-5	0.03852430368792188
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	9.399953940225694E-6	0.0
2	0.0	1.8799907880451387E-5	0.0	9.399953940225694E-6	0.0
3	0.0	1.8799907880451387E-5	0.0	9.399953940225694E-6	0.0
4	0.0	3.7599815760902774E-5	0.0	2.8199861820677077E-5	9.399953940225694E-6
5	2.8199861820677077E-5	4.6999769701128465E-5	0.0	3.7599815760902774E-5	9.399953940225694E-6
6	2.8199861820677077E-5	4.6999769701128465E-5	0.0	4.6999769701128465E-5	1.8799907880451387E-5
7	2.8199861820677077E-5	4.6999769701128465E-5	0.0	4.6999769701128465E-5	1.8799907880451387E-5
8	2.8199861820677077E-5	5.6399723641354155E-5	0.0	4.6999769701128465E-5	1.8799907880451387E-5
9	2.8199861820677077E-5	1.22199401222934E-4	0.0	6.579967758157985E-5	2.8199861820677077E-5
10-11	3.7599815760902774E-5	1.22199401222934E-4	0.0	7.98996084919184E-5	2.8199861820677077E-5
12-13	4.229979273101562E-5	1.22199401222934E-4	0.0	8.459958546203124E-5	2.8199861820677077E-5
14-15	5.169974667124131E-5	1.8799907880451386E-4	0.0	1.0339949334248262E-4	3.7599815760902774E-5
16-17	5.6399723641354155E-5	1.9739903274473955E-4	0.0	1.5509924001372392E-4	5.6399723641354155E-5
18-19	5.6399723641354155E-5	2.349988485056423E-4	0.0	2.9139857214699645E-4	6.579967758157985E-5
20-21	6.1099700611467E-5	2.6789868729643225E-4	0.0	5.592972594434287E-4	7.519963152180555E-5
22-23	6.579967758157985E-5	2.960985491171094E-4	0.0	0.0013629933213327255	7.98996084919184E-5
24-25	7.98996084919184E-5	3.1489845699756076E-4	0.0	0.0029421855832906423	8.459958546203124E-5
26-27	8.929956243214408E-5	3.2899838790789927E-4	0.0	0.007980560895251614	8.459958546203124E-5
28-29	9.399953940225693E-5	3.2899838790789927E-4	0.0	0.03630262211715163	8.459958546203124E-5
30-31	1.1279944728270831E-4	3.4779829578835065E-4	0.0	0.055290529076407524	8.459958546203124E-5
32-33	1.503992630436111E-4	3.4779829578835065E-4	0.0	0.06992625736133892	9.399953940225693E-5
34-35	1.7859912486428817E-4	3.900980885193663E-4	0.0	0.08993875930007944	1.0339949334248262E-4
36-37	1.9739903274473955E-4	4.2299792731015617E-4	0.0	0.11604243139208618	1.0339949334248262E-4
38-39	2.020990097148524E-4	4.4179783519060755E-4	0.0	0.16422189531271297	1.0339949334248262E-4
40-41	2.1619894062519093E-4	4.7939765095151036E-4	0.0	0.26740988969154056	1.0339949334248262E-4
42-43	2.1619894062519093E-4	4.981975588319617E-4	0.0	0.3095733830904228	1.1279944728270831E-4
44-45	2.3029887153552946E-4	5.404973515629773E-4	0.0	0.3711430813989012	1.22199401222934E-4
46-47	2.4909877941598087E-4	5.780971673238801E-4	0.0	0.4397298453237579	1.22199401222934E-4
48-49	2.631987103263194E-4	5.874971212641058E-4	0.0	0.49396757955886017	1.4099930910338538E-4
50-51	2.6789868729643225E-4	6.532967988456856E-4	0.0	0.5481207142085004	1.4099930910338538E-4
52-53	2.7259866426654507E-4	6.626967527859114E-4	0.0	0.6235318446939611	1.503992630436111E-4
54-55	2.7259866426654507E-4	7.002965685468141E-4	0.0	0.7759191979959298	1.6449919395394963E-4
56-57	2.7259866426654507E-4	7.237964533973783E-4	0.0	0.9370767083241291	1.7389914789417532E-4
58-59	2.772986412366579E-4	7.284964303674911E-4	0.0	1.083424591219503	1.8329910183440101E-4
60-61	2.913985721469965E-4	7.472963382479426E-4	0.0	1.7554695981989688	1.8799907880451386E-4
62-63	2.913985721469965E-4	7.519963152180554E-4	0.0	1.772102816696198	1.8799907880451386E-4
64-65	2.913985721469965E-4	7.989960849191839E-4	0.0	1.789342332222572	1.8799907880451386E-4
66-67	3.007985260872222E-4	8.224959697697481E-4	0.0	1.8004107779871878	1.8799907880451386E-4
68-69	3.007985260872222E-4	8.27195946739861E-4	0.0	1.8114604238439231	1.926990557746267E-4
70-71	3.05498503057335E-4	8.27195946739861E-4	0.0	1.821283375711459	1.9739903274473955E-4
72-73	3.101984800274479E-4	8.27195946739861E-4	0.0	1.829028937758205	2.349988485056423E-4
74-75	3.101984800274479E-4	8.318959237099738E-4	0.0	1.83590500406548	2.44398802445868E-4
76-77	3.101984800274479E-4	8.553958085605381E-4	0.0	1.841483876729004	2.44398802445868E-4
78-79	3.195984339676736E-4	8.694957394708765E-4	0.0	1.8465128520870246	2.44398802445868E-4
80-81	3.195984339676736E-4	8.741957164409895E-4	0.0	1.8490414396969455	2.537987563860937E-4
82-83	3.195984339676736E-4	8.788956934111023E-4	0.0	1.8499203353903564	2.631987103263194E-4
84-85	3.242984109377864E-4	8.976956012915537E-4	0.0	1.8510436298862136	2.631987103263194E-4
86-87	3.2899838790789927E-4	9.117955322018922E-4	0.0	1.8516734268002086	2.631987103263194E-4
88-89	3.2899838790789927E-4	9.305954400823436E-4	0.0	1.8519225255796246	2.631987103263194E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11205	0.0	46.63331	1
GTATCAA	19690	0.0	33.136845	1
GACCGAG	16020	0.0	27.80676	90-91
ACCGAGG	16370	0.0	27.561277	92-93
AGACCGA	15795	0.0	27.1765	90-91
TCAACGC	23455	0.0	27.07494	4
ATCAACG	23750	0.0	26.738768	3
GAGACCG	16505	0.0	26.710274	88-89
CAACGCA	24350	0.0	26.079782	5
ACGAGAC	16640	0.0	26.056349	86-87
CGAGACC	16770	0.0	25.474321	88-89
TATCAAC	25355	0.0	25.374407	2
AACGCAG	25110	0.0	25.338	6
CACGAGA	16920	0.0	24.507608	86-87
CCACGAG	17625	0.0	24.228666	84-85
GCCCACG	17725	0.0	23.790163	82-83
CCCACGA	17210	0.0	23.685907	84-85
ACGCAGA	29610	0.0	21.447104	7
CCGAGCC	19260	0.0	21.397558	78-79
CGAGCCC	18960	0.0	21.08611	80-81
>>END_MODULE