FastQCFastQC Report
Fri 27 May 2016
SRR2139737_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139737_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9290157
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94950.045.0500531
GTATCAA156850.033.8758431
TCAACGC179650.028.6877214
ATCAACG182100.028.2782023
CAACGCA187600.027.4720125
TATCAAC196000.026.5590522
AACGCAG198800.025.9462836
GTACATG272150.022.2359811
ACGCAGA232800.022.0270887
TACATGG268150.021.9079442
CGCAGAG234700.021.867168
ACATGGG272100.020.9712453
CATGGGG200300.018.9851444
GCAGAGT291200.017.5797339
TATCTCG175500.017.47704794-95
GTGGTAT52550.017.0846961
GAGTACT146100.016.9877812-13
CAGAGTA278350.016.7087910-11
AGAGTAC252450.016.60209310-11
GAGTACA192300.015.3530051
Produced by FastQC (version 0.11.5)