##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2139737_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9290157 Sequences flagged as poor quality 0 Sequence length 19-101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.83836807063648 31.0 31.0 34.0 30.0 34.0 2 32.03126341137184 33.0 31.0 34.0 30.0 34.0 3 32.0950443571621 33.0 31.0 34.0 30.0 34.0 4 35.77341168722983 37.0 35.0 37.0 35.0 37.0 5 35.534250712878155 37.0 35.0 37.0 33.0 37.0 6 35.56415774243643 37.0 35.0 37.0 33.0 37.0 7 35.524862066378425 37.0 35.0 37.0 33.0 37.0 8 35.51612938295876 37.0 35.0 37.0 33.0 37.0 9 37.29589801334897 39.0 37.0 39.0 34.0 39.0 10-11 37.14774874095239 39.0 37.0 39.0 33.0 39.0 12-13 37.1529877266875 39.0 37.0 39.0 33.0 39.0 14-15 38.285770358886296 40.0 38.0 41.0 33.5 41.0 16-17 38.21076947354065 40.0 38.0 41.0 33.0 41.0 18-19 38.27039666821562 40.0 38.0 41.0 33.5 41.0 20-21 38.31014074732777 40.0 38.0 41.0 34.0 41.0 22-23 38.29404321576175 40.0 38.0 41.0 34.0 41.0 24-25 38.247766064064294 40.0 38.0 41.0 34.0 41.0 26-27 38.13187038870856 40.0 38.0 41.0 33.5 41.0 28-29 37.96449087492641 40.0 37.0 41.0 33.0 41.0 30-31 37.87175903612348 40.0 37.0 41.0 33.0 41.0 32-33 37.77839499067586 40.0 37.0 41.0 33.0 41.0 34-35 37.70742224544324 40.0 37.0 41.0 33.0 41.0 36-37 37.66117307624478 39.5 37.0 41.0 33.0 41.0 38-39 37.56321217878019 39.0 37.0 41.0 32.0 41.0 40-41 37.551355934885635 39.0 37.0 41.0 32.0 41.0 42-43 37.4957824396064 39.0 37.0 41.0 32.0 41.0 44-45 37.417188002104865 39.0 36.0 41.0 32.0 41.0 46-47 37.356317209465274 39.0 36.0 40.5 31.5 41.0 48-49 37.366075910150315 39.0 36.0 40.0 32.0 41.0 50-51 37.23039857180082 39.0 36.0 40.0 31.0 41.0 52-53 37.04297446845921 39.0 35.5 40.0 31.0 41.0 54-55 36.85706892405411 39.0 35.0 40.0 31.0 41.0 56-57 36.73109859277447 39.0 35.0 40.0 31.0 41.0 58-59 36.54694203646104 38.0 35.0 40.0 30.5 41.0 60-61 36.361509773713586 38.0 35.0 40.0 30.0 41.0 62-63 36.21639883280392 38.0 34.5 40.0 30.0 41.0 64-65 35.97139121028097 37.5 34.0 40.0 30.0 41.0 66-67 35.82297655244339 37.0 34.0 40.0 30.0 41.0 68-69 35.540887589977636 36.5 34.0 39.0 29.0 41.0 70-71 35.21202284351408 36.0 34.0 39.0 29.0 40.5 72-73 34.914341910003564 36.0 34.0 39.0 29.0 40.0 74-75 34.558423878110375 35.0 33.0 38.0 29.0 40.0 76-77 32.42410872167647 33.5 30.5 35.5 25.5 38.5 78-79 33.66736026809737 35.0 32.5 36.0 28.0 39.0 80-81 33.643807060477805 35.0 33.0 36.0 28.5 39.0 82-83 33.47186570240393 35.0 33.0 36.0 29.0 37.0 84-85 33.28639857851728 35.0 33.0 35.5 29.0 37.0 86-87 33.05782461419058 35.0 32.0 35.0 28.0 36.5 88-89 32.904461466199606 34.0 32.0 35.0 28.0 36.0 90-91 32.76811645683627 34.0 32.0 35.0 27.5 36.0 92-93 32.76205055514659 34.0 32.0 35.0 28.5 36.0 94-95 32.73121361623801 34.0 32.0 35.0 28.5 35.0 96-97 32.749403118233644 34.0 32.0 35.0 29.0 35.0 98-99 32.9052165747895 34.0 32.0 35.0 29.0 35.0 100-101 31.671308679892014 33.0 30.0 34.5 26.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 6.0 19 14.0 20 62.0 21 329.0 22 1540.0 23 5469.0 24 14194.0 25 30690.0 26 55916.0 27 89660.0 28 130630.0 29 177364.0 30 229293.0 31 290107.0 32 368935.0 33 479617.0 34 650248.0 35 925449.0 36 1434943.0 37 2104236.0 38 1826247.0 39 470573.0 40 4635.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.361313915362246 20.314005457604214 13.665807800664725 24.65887282636881 2 15.083222489496073 21.141695291233233 40.34858839471752 23.426493824553177 3 18.782605213691063 25.409098722868613 30.114335481458415 25.693960581981905 4 12.831508193350919 17.69800396225897 37.6585643619509 31.81192348243921 5 13.26597602171847 37.729631479855506 35.62026992654699 13.384122571879034 6 30.904967483326708 37.323233611660164 18.18195322210378 13.58984568290934 7 27.212812442243976 32.05407615823931 22.271302842352394 18.461808557164318 8 24.922059227344647 36.61092002888677 20.880333801683022 17.586686942085564 9 25.468320933650528 16.46978624796115 20.629371495013483 37.432521323374836 10-11 24.618858939541212 26.42565281648373 28.51095732783072 20.444530916144338 12-13 25.19467739520499 23.664346171031504 28.765150649943656 22.375825783819852 14-15 22.16920553656951 25.301784458540368 25.899169411238155 26.62984059365197 16-17 22.208914230405362 28.68011810779947 26.631928825314795 22.479038836480374 18-19 22.346928209092713 27.818188100517133 28.02784636320012 21.80703732719003 20-21 22.85840908205276 26.93758015592743 28.014944714073835 22.189066047945975 22-23 22.552956612068723 27.12819860800593 27.866214489472085 22.45263029045326 24-25 22.44844809219511 27.24647380562474 27.931642992690648 22.373435109489503 26-27 22.479147216888983 27.335524636049907 27.94124807944106 22.24408006762005 28-29 22.394136215279246 27.232459297463702 28.007252655268992 22.366151831988056 30-31 22.404915931507947 27.032669501840108 28.07619816872669 22.486216397925258 32-33 22.128197386332815 27.355607454198534 27.99789641794105 22.518298741527598 34-35 22.409482661180785 27.280063522480262 27.98918357056774 22.321270245771217 36-37 22.3247891449507 27.29589377053947 28.048618709254335 22.330698375255494 38-39 22.258082000843284 27.2446131161247 28.05807347938285 22.439231403649167 40-41 22.412719169836897 27.06923055466543 28.053332800355278 22.464717475142393 42-43 22.536149403575312 27.069906323870768 27.969485340957046 22.42445893159688 44-45 22.28868434866717 27.24849077635616 27.83435413351048 22.628470741466185 46-47 22.51249488130746 27.11364848963941 27.552125983262844 22.82173064579029 48-49 22.360493825485655 27.309070298745237 27.572735036807078 22.757700838962034 50-51 22.241351947151298 27.411316606752013 27.678673865160068 22.668657580936625 52-53 22.364831823576868 27.318399842104384 27.568756542808124 22.74801179151062 54-55 22.274413179065554 27.142477062634374 27.525240735454325 23.057869022845743 56-57 22.411462826674377 27.06727483340645 27.592408396357516 22.928853943561656 58-59 22.12615211042903 27.043463980478638 27.894863547101384 22.93552036199095 60-61 22.334581787258116 26.931174267434248 27.86229172548081 22.871952219826824 62-63 22.045232247565835 27.459490505217047 27.84414104774554 22.651136199471576 64-65 21.779406007431298 27.539573152459955 27.693762642508556 22.98725819760019 66-67 21.843620959835906 27.90874280792115 27.328672841903582 22.918963390339357 68-69 21.92599717261828 28.132575208444493 26.9914533898164 22.94997422912082 70-71 21.905024320489673 27.794031160418875 26.953066294109796 23.347878224981656 72-73 21.87876588749871 27.48034796808227 26.880554785632178 23.760331358786846 74-75 22.18889688443809 27.199418561157966 26.997379831747075 23.614304722656865 76-77 22.546725295428153 27.043272937249153 26.86175350958562 23.54824825773707 78-79 22.669418935504556 27.001962035625894 26.323169833780717 24.005449195088833 80-81 22.628818845961074 27.316264015578266 26.2575466876757 23.797370450784964 82-83 22.56381538802834 27.442243211641344 26.33648306043131 23.65745833989901 84-85 22.595649456498226 27.456287893864896 26.312667774681227 23.635394874955654 86-87 22.828062002869924 27.670066448607162 26.30412744446741 23.197744104055506 88-89 22.949374145874167 27.44528375373522 26.58892816769667 23.016413932693943 90-91 22.714466502939246 27.480140676469166 26.77380878841153 23.031584032180064 92-93 22.518239210959027 27.106656294483184 26.731056313453237 23.64404818110455 94-95 22.411481267439182 27.124419271273855 27.252227221318048 23.211872239968915 96-97 22.058868547319104 27.294955719559926 27.48542409326214 23.16075163985883 98-99 22.365049481727254 27.488043145642955 27.78308503152509 22.363822341104704 100-101 23.334930733417067 30.266454067673347 24.787968295040542 21.61064690386904 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1184.0 1 1075.0 2 1530.5 3 2255.5 4 3057.5 5 5042.5 6 6837.5 7 8084.0 8 8950.5 9 8901.0 10 8215.0 11 7296.0 12 7611.5 13 10048.5 14 16183.0 15 25029.5 16 32512.5 17 36826.5 18 38000.0 19 36810.0 20 34334.5 21 31598.0 22 29999.0 23 31770.5 24 37104.0 25 45631.5 26 57956.0 27 72346.0 28 89200.5 29 108672.5 30 127056.5 31 146177.0 32 166533.0 33 185951.5 34 206100.5 35 225841.5 36 247686.0 37 269268.5 38 288188.0 39 305065.0 40 322812.5 41 340916.0 42 354438.0 43 366993.5 44 378143.5 45 389502.0 46 398488.0 47 403724.0 48 416688.0 49 422972.5 50 416143.0 51 406382.5 52 391159.0 53 372248.5 54 349534.0 55 322582.0 56 293587.0 57 262920.5 58 230945.0 59 196955.5 60 162332.0 61 131469.5 62 104205.0 63 80300.0 64 59454.0 65 43811.5 66 32792.0 67 24513.0 68 18429.0 69 13331.0 70 9408.5 71 6607.0 72 4530.0 73 3134.0 74 2257.5 75 1615.0 76 1137.5 77 768.0 78 512.5 79 354.0 80 243.5 81 163.5 82 113.0 83 81.5 84 65.0 85 54.5 86 48.0 87 45.5 88 37.5 89 29.0 90 22.5 91 16.0 92 13.5 93 9.0 94 4.5 95 3.5 96 3.0 97 2.5 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 6.458448441721707E-4 3 4.3056322944811374E-4 4 2.152816147240569E-5 5 0.0 6 0.0 7 0.0 8 9.687672662582558E-4 9 0.0 10-11 2.906301798774768E-4 12-13 4.736195523929251E-4 14-15 0.0 16-17 0.0 18-19 4.1979914871191095E-4 20-21 0.0 22-23 0.0 24-25 1.1845274653422071E-4 26-27 0.0 28-29 5.928859613497643E-4 30-31 3.7234244626181137E-4 32-33 0.0010814102974483909 34-35 0.0011546845824562972 36-37 4.897149788086726E-5 38-39 0.0017392665512953186 40-41 4.2828410170978704E-4 42-43 1.27071507280783E-4 44-45 1.2796497631980294E-4 46-47 6.729583033913622E-5 48-49 3.9568065947982465E-5 50-51 4.557256285624215E-5 52-53 3.4384921661692605E-5 54-55 3.732697082673268E-4 56-57 5.238356141673241E-4 58-59 4.7893773226676786E-4 60-61 5.333097749537092E-4 62-63 7.027073146151233E-5 64-65 8.284342297188945E-5 66-67 1.4960722418576715E-4 68-69 7.868964549588338E-5 70-71 1.4079828710925567E-4 72-73 0.0 74-75 1.2520214669096674E-5 76-77 7.471964145716724E-4 78-79 1.2810782374336314E-5 80-81 2.0107811599405173E-4 82-83 1.3142296905514772E-5 84-85 6.656074190200382E-6 86-87 1.3491553275782863E-5 88-89 1.3689968887492208E-5 90-91 7.652108437611282E-5 92-93 1.1325392251421443E-4 94-95 3.5361758365599737E-4 96-97 9.612510105151247E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 284.0 20-21 850.0 22-23 1652.0 24-25 3794.0 26-27 5124.0 28-29 8770.0 30-31 17431.0 32-33 22221.0 34-35 30317.0 36-37 44072.0 38-39 36808.0 40-41 54464.0 42-43 60350.0 44-45 69989.0 46-47 71051.0 48-49 69192.0 50-51 64987.0 52-53 16850.0 54-55 20809.0 56-57 20744.0 58-59 23087.0 60-61 87508.0 62-63 88183.0 64-65 90618.0 66-67 97910.0 68-69 92270.0 70-71 92310.0 72-73 88874.0 74-75 92460.0 76-77 87333.0 78-79 96440.0 80-81 100010.0 82-83 97291.0 84-85 98252.0 86-87 105227.0 88-89 114993.0 90-91 122163.0 92-93 131207.0 94-95 153168.0 96-97 234541.0 98-99 913866.0 100-101 5662687.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.1768219922847 #Duplication Level Percentage of deduplicated Percentage of total 1 88.7346949339882 74.69404619998012 2 7.874429669027637 13.256889290810095 3 1.9500798403583406 4.92454570777761 4 0.6901592761487341 2.323816581387842 5 0.3086637768026226 1.2991167897690328 6 0.15263913797679857 0.7709206527917255 7 0.08650156876584192 0.5096999009238955 8 0.05471191621453849 0.3684380185638402 9 0.03345207493278732 0.25343004212008263 >10 0.11253928901125844 1.4445260154985153 >50 0.0018398318522762221 0.10376621016192837 >100 2.6801210854223204E-4 0.041024825556260115 >500 2.0672812376866496E-5 0.009779764659103936 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.0764080736202843E-5 0.0 1.0764080736202843E-5 0.0 3 0.0 1.0764080736202843E-5 0.0 1.0764080736202843E-5 0.0 4 0.0 5.382040368101422E-5 0.0 1.0764080736202843E-5 0.0 5 2.1528161472405686E-5 5.382040368101422E-5 0.0 1.0764080736202843E-5 0.0 6 3.229224220860853E-5 5.382040368101422E-5 0.0 1.0764080736202843E-5 0.0 7 3.229224220860853E-5 5.382040368101422E-5 0.0 2.1528161472405686E-5 0.0 8 3.229224220860853E-5 6.458448441721706E-5 0.0 2.1528161472405686E-5 1.0764080736202843E-5 9 3.229224220860853E-5 8.611264588962274E-5 0.0 9.687672662582559E-5 2.1528161472405686E-5 10-11 7.534856515341991E-5 8.611264588962274E-5 0.0 1.453150899387384E-4 3.229224220860853E-5 12-13 8.611264588962274E-5 8.611264588962274E-5 0.0 1.8298937251544833E-4 3.767428257670995E-5 14-15 9.149468625772417E-5 1.7222529177924548E-4 0.0 2.1528161472405687E-4 7.53485651534199E-5 16-17 1.0764080736202843E-4 2.1528161472405687E-4 0.0 2.5833793766886825E-4 1.0225876699392701E-4 18-19 1.3993304957063697E-4 2.475738569326654E-4 0.0 2.960122202455782E-4 1.3455100920253555E-4 20-21 1.6146121104304264E-4 2.906301798774768E-4 0.0 4.574734312886208E-4 1.668432514111441E-4 22-23 2.0451753398785402E-4 3.0139426061367964E-4 0.0 6.458448441721706E-4 1.991354936197526E-4 24-25 2.475738569326654E-4 3.7136078539899806E-4 0.0 8.395982974238218E-4 2.1528161472405687E-4 26-27 2.744840587731725E-4 4.0903506797570805E-4 0.0 0.0012540154057676311 2.1528161472405687E-4 28-29 2.7986609914127395E-4 4.1441710834380944E-4 0.0 0.003950417630186443 2.206636550921583E-4 30-31 2.906301798774768E-4 4.682375120248237E-4 0.0 0.011748994123565403 2.3142773582836114E-4 32-33 3.0139426061367964E-4 4.736195523929251E-4 0.0 0.027055516930445847 2.3680977619646256E-4 34-35 3.175403817179839E-4 5.86642400123055E-4 0.0 0.04687757160616338 2.475738569326654E-4 36-37 3.2292242208608533E-4 6.996652478531848E-4 0.0 0.07603746632053689 2.475738569326654E-4 38-39 3.336865028222881E-4 7.104293285893876E-4 0.0 0.13417426637676844 2.691020184050711E-4 40-41 3.6597874503089666E-4 7.750138130066047E-4 0.0 0.38464904306784053 2.691020184050711E-4 42-43 3.767428257670995E-4 8.019240148471119E-4 0.0 0.43859861571768916 2.691020184050711E-4 44-45 3.8750690650330235E-4 8.880366607367346E-4 0.0 0.5206693492908677 2.691020184050711E-4 46-47 3.982709872395052E-4 9.58003185522053E-4 0.0 0.6210067278733825 3.0139426061367964E-4 48-49 3.982709872395052E-4 9.633852258901544E-4 0.0 0.7013067701654557 3.283044624541867E-4 50-51 3.982709872395052E-4 0.0010279697103073716 0.0 0.7816229585786333 3.390685431903896E-4 52-53 3.982709872395052E-4 0.001033351750675473 0.0 0.9861566386875916 3.7136078539899806E-4 54-55 4.0365302760760665E-4 0.0010494978717797772 0.0 1.5959149021916423 3.982709872395052E-4 56-57 4.0903506797570805E-4 0.0010656439928840816 0.0 2.2666785932681224 4.2518118908001234E-4 58-59 4.0903506797570805E-4 0.0010764080736202844 0.0 2.9686312082777504 4.3056322944811374E-4 60-61 4.0903506797570805E-4 0.0011087003158288928 0.0 3.5121903752541535 4.3056322944811374E-4 62-63 4.1441710834380944E-4 0.0011409925580375014 0.0 3.533966110583492 4.413273101843166E-4 64-65 4.197991487119109E-4 0.0012809256076081383 0.0 3.545591317778591 4.413273101843166E-4 66-67 4.197991487119109E-4 0.0012809256076081383 0.0 3.552975477163626 4.413273101843166E-4 68-69 4.197991487119109E-4 0.0012970717287124427 0.0 3.55891724973001 4.413273101843166E-4 70-71 4.197991487119109E-4 0.0013024537690805441 0.0 3.5646760329238782 4.628554716567223E-4 72-73 4.197991487119109E-4 0.0013024537690805441 0.0 3.56920771091382 4.8438363312912797E-4 74-75 4.197991487119109E-4 0.001313217849816747 0.0 3.573195802826583 4.8438363312912797E-4 76-77 4.3056322944811374E-4 0.0013562741727615583 0.0 3.577006287407199 4.8438363312912797E-4 78-79 4.413273101843166E-4 0.0013993304957063697 0.0 3.580208601426219 4.8438363312912797E-4 80-81 4.413273101843166E-4 0.0013993304957063697 0.0 3.581844741698122 4.897656734972294E-4 82-83 4.5209139092051943E-4 0.0013993304957063697 0.0 3.582549788986343 5.059117946015336E-4 84-85 4.5209139092051943E-4 0.0014154766168106739 0.0 3.5832440721938283 5.059117946015336E-4 86-87 4.5209139092051943E-4 0.0014208586571787753 0.0 3.5835992868581235 5.059117946015336E-4 88-89 4.5209139092051943E-4 0.0014746790608597897 0.0 3.5837930403113747 5.11293834969635E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9495 0.0 45.050053 1 GTATCAA 15685 0.0 33.875843 1 TCAACGC 17965 0.0 28.687721 4 ATCAACG 18210 0.0 28.278202 3 CAACGCA 18760 0.0 27.472012 5 TATCAAC 19600 0.0 26.559052 2 AACGCAG 19880 0.0 25.946283 6 GTACATG 27215 0.0 22.235981 1 ACGCAGA 23280 0.0 22.027088 7 TACATGG 26815 0.0 21.907944 2 CGCAGAG 23470 0.0 21.86716 8 ACATGGG 27210 0.0 20.971245 3 CATGGGG 20030 0.0 18.985144 4 GCAGAGT 29120 0.0 17.579733 9 TATCTCG 17550 0.0 17.477047 94-95 GTGGTAT 5255 0.0 17.084696 1 GAGTACT 14610 0.0 16.98778 12-13 CAGAGTA 27835 0.0 16.70879 10-11 AGAGTAC 25245 0.0 16.602093 10-11 GAGTACA 19230 0.0 15.353005 1 >>END_MODULE