FastQCFastQC Report
Fri 27 May 2016
SRR2139736_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139736_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15951494
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA180050.052.237121
CTTATAC358850.043.789981
TATACAC433050.038.116443
TTATACA427000.037.52322
GTATCAA302650.033.312391
TACACAT541950.030.3387415
ACACATC569000.027.9028786
ATACACA640150.026.9023974
ACATCTC589650.026.8966438
TCAACGC371350.026.2729594
ATCAACG375600.026.044093
CACATCT616400.025.5765447
CAACGCA385100.025.3465
TATCAAC398800.024.819032
AACGCAG396050.024.6668626
CATCTCC666550.023.6200839
ACGCAGA465150.021.0393287
CGCAGAG473850.020.6259258
GTACATG479800.019.0223081
TACATGG485700.018.4826052
Produced by FastQC (version 0.11.5)