##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139736_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15951494
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3629013056708	34.0	31.0	34.0	31.0	34.0
2	32.49593567850134	34.0	31.0	34.0	31.0	34.0
3	32.563806374500096	34.0	31.0	34.0	31.0	34.0
4	36.100018970009955	37.0	35.0	37.0	35.0	37.0
5	36.004168386986194	37.0	35.0	37.0	35.0	37.0
6	36.02158857345901	37.0	35.0	37.0	35.0	37.0
7	35.93471138189313	37.0	35.0	37.0	35.0	37.0
8	35.906507377929614	37.0	35.0	37.0	35.0	37.0
9	37.597860614184476	39.0	37.0	39.0	35.0	39.0
10-11	37.490390053746694	39.0	37.0	39.0	35.0	39.0
12-13	37.45088942766113	39.0	37.0	39.0	34.0	39.0
14-15	38.67302460822792	40.0	38.0	41.0	34.0	41.0
16-17	38.703107840557124	40.0	38.0	41.0	34.0	41.0
18-19	38.69209304156714	40.0	38.0	41.0	34.0	41.0
20-21	38.67429416761847	40.0	38.0	41.0	34.0	41.0
22-23	38.570416600951404	40.0	38.0	41.0	34.0	41.0
24-25	38.48086104495932	40.0	38.0	41.0	34.0	41.0
26-27	38.31516428887758	40.0	38.0	41.0	34.0	41.0
28-29	38.143875449070045	40.0	38.0	41.0	33.0	41.0
30-31	38.01525137591928	40.0	37.5	41.0	33.0	41.0
32-33	38.220779565032046	40.0	38.0	41.0	34.0	41.0
34-35	38.183105482240215	40.0	38.0	41.0	33.0	41.0
36-37	38.07974246952987	40.0	38.0	41.0	33.0	41.0
38-39	37.92023753240292	40.0	37.0	41.0	33.0	41.0
40-41	37.90199516739614	40.0	37.0	41.0	33.0	41.0
42-43	37.81858697687453	40.0	37.0	41.0	33.0	41.0
44-45	37.845938718183916	40.0	37.0	41.0	33.0	41.0
46-47	37.956987166644595	40.0	37.0	41.0	33.0	41.0
48-49	37.913913415361534	40.0	37.0	41.0	33.0	41.0
50-51	37.74765761781015	40.0	37.0	41.0	32.5	41.0
52-53	37.63436783643536	40.0	36.5	41.0	32.0	41.0
54-55	37.54047173787808	39.5	36.0	41.0	32.0	41.0
56-57	37.35910763802747	39.0	36.0	41.0	32.0	41.0
58-59	37.23372894972068	39.0	35.5	41.0	31.0	41.0
60-61	36.991182479553046	39.0	35.0	40.5	31.0	41.0
62-63	36.77449110504449	38.5	35.0	40.0	31.0	41.0
64-65	36.55939156298309	38.0	35.0	40.0	31.0	41.0
66-67	36.27946191417819	37.5	35.0	40.0	31.0	41.0
68-69	35.97191675554525	37.0	34.5	40.0	30.0	41.0
70-71	35.66463892241425	36.5	34.0	39.0	30.0	41.0
72-73	35.33936043493959	36.0	34.0	39.0	30.0	40.0
74-75	34.937100865328865	35.5	34.0	38.5	29.0	40.0
76-77	33.934821985585806	35.0	32.5	37.0	28.5	39.0
78-79	34.22568057188411	35.0	33.0	37.0	29.0	39.0
80-81	34.10305359189759	35.0	33.0	36.5	29.0	39.0
82-83	33.85559277584444	35.0	33.0	36.0	29.0	37.5
84-85	33.61144951108825	35.0	33.0	36.0	29.0	37.0
86-87	33.40187048225138	35.0	33.0	35.0	29.0	37.0
88-89	33.17225931684953	35.0	33.0	35.0	29.0	36.0
90-91	33.051165392155184	35.0	33.0	35.0	29.0	36.0
92-93	32.96744514935258	34.0	33.0	35.0	29.0	36.0
94-95	32.92169151529732	34.0	32.0	35.0	29.0	35.5
96-97	32.88182500314045	34.0	32.0	35.0	29.0	35.0
98-99	32.97919953809644	34.0	32.0	35.0	29.0	35.0
100-101	32.17751596692494	33.5	30.5	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	0.0
19	23.0
20	111.0
21	669.0
22	3126.0
23	10043.0
24	24887.0
25	51317.0
26	91892.0
27	144871.0
28	204681.0
29	272420.0
30	342875.0
31	425613.0
32	528356.0
33	672739.0
34	904640.0
35	1274557.0
36	2015409.0
37	3440036.0
38	3993497.0
39	1499236.0
40	50494.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	36.76051910874305	22.169120961334404	16.13609985371903	24.934260076203522
2	13.547409938466428	22.348744989093472	40.538251839722456	23.565593232717642
3	15.95830273005868	27.66127497358861	33.005064366554215	23.37535792979849
4	11.920707865984214	20.62492077544586	38.259620070696826	29.1947512878731
5	12.612498866877297	38.21245834402721	36.13570616018788	13.039336628907611
6	28.191453414959128	39.05813461735935	19.143529753388616	13.606882214292906
7	24.74402084218569	33.29211044432578	23.29134813328457	18.67252058020396
8	24.88930503939004	35.95063885552037	21.669406013004174	17.49065009208542
9	24.92504463845205	16.299971651558153	20.67227684127894	38.102706868710854
10-11	23.60579203427591	26.654785438906224	29.33715174265182	20.402270784166046
12-13	24.73103773226508	24.228348140932752	28.659666611791973	22.380947515010195
14-15	22.241687140198188	25.749049148386767	25.706325866737213	26.30293784467783
16-17	22.188960619548276	29.140742499777296	26.3140133653947	22.356283515279728
18-19	22.18876380362204	27.663511608284235	27.653390286897928	22.494334301195792
20-21	23.02896647606625	26.984736805000477	27.400499650184685	22.58579706874859
22-23	22.673298898673394	27.813153297692267	27.25196684724218	22.26158095639216
24-25	22.90754496941144	28.012194339802825	27.2445926075304	21.83566808325533
26-27	22.8503131164625	28.10235904031495	27.26821873005606	21.779109113166488
28-29	22.239232910023233	27.214420866343175	27.618401926458034	22.927944297175554
30-31	23.180863087542214	27.336094074254152	27.607411305114233	21.8756315330894
32-33	23.05262089510462	27.457610236395446	27.826023641120962	21.663745227378968
34-35	22.625534851813782	27.690203233732035	28.044941934980557	21.639319979473626
36-37	22.82122676946255	27.234457438978488	27.643171274720107	22.301144516838853
38-39	22.27533961501495	26.87516207003468	28.1825384527863	22.666959862164074
40-41	22.2529036781875	26.74899187808906	28.445924521928966	22.552179921794473
42-43	22.509321633390726	26.713851923988745	28.468420660033072	22.30840578258745
44-45	22.10671107760475	27.19166108997047	28.183591030386413	22.51803680203837
46-47	22.818854941395696	26.72652939814131	27.465329125118366	22.989286535344633
48-49	22.420250469708158	26.826881611131615	27.850687848391882	22.902180070768352
50-51	22.219808615279987	27.22550179917358	27.863008272925484	22.691681312620958
52-53	22.24893777212089	27.45010893604245	28.118733688947557	22.1822196028891
54-55	22.160171297224508	27.482790809521813	27.66608674788354	22.690951145370136
56-57	22.58966630227465	27.72543819712717	27.149110652200154	22.53578484839803
58-59	22.087854271863016	27.951190288174022	27.817694033153213	22.143261406809753
60-61	22.581124797715322	28.235017079816227	27.056896284916576	22.126961837551878
62-63	22.171807634932676	28.550522392691153	27.061799745460473	22.215870226915698
64-65	22.228028460863058	28.26939755141004	27.104620155451336	22.397953832275565
66-67	22.37241725755933	27.999356481026133	27.12302051180899	22.50520574960555
68-69	22.39707984902079	27.727057540740297	27.240536171972472	22.635326438266443
70-71	22.42560900042445	27.506315683344745	27.32653388020077	22.741541436030033
72-73	22.41563236982225	27.599701220376428	27.233117569397614	22.751548840403707
74-75	22.35812915564824	27.652083007114058	27.23982263913983	22.749965198097872
76-77	22.477834570529758	27.644245021697788	27.2108877852911	22.667032622481354
78-79	22.454214749150932	27.664162215903648	27.142122004992075	22.73950102995335
80-81	22.47197768367143	27.678270496063966	27.17033292924638	22.679418891018223
82-83	22.455260975988654	27.681316446417814	27.257047886922024	22.606374690671508
84-85	22.485864367077586	27.71411146058148	27.266255192023948	22.53376898031699
86-87	22.353623187484065	27.735706272746256	27.35030925650048	22.56036128326919
88-89	22.391364635194826	27.729684257843708	27.30855373912287	22.570397367838595
90-91	22.425758390851243	27.735873032356366	27.37533370552666	22.463034871265723
92-93	22.41064744161268	27.722301742148925	27.384941141690415	22.482109674547974
94-95	22.279311652503036	27.75928804928848	27.45601703305973	22.505383265148755
96-97	22.162032831321287	27.821839037706354	27.577296089648364	22.438832041324
98-99	22.290044544809565	28.407212180434737	27.66715608228151	21.635587192474183
100-101	21.769847550483824	31.616867807474325	25.21513850315519	21.398146138886666
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1368.0
1	1402.5
2	2355.0
3	3470.0
4	4524.0
5	6545.5
6	8115.0
7	9316.0
8	10097.5
9	10065.5
10	9690.0
11	9532.0
12	11309.0
13	16176.5
14	27355.0
15	42425.5
16	54075.5
17	60131.5
18	61676.0
19	59343.5
20	53792.5
21	47196.5
22	43093.5
23	44793.5
24	52876.5
25	68049.0
26	90268.0
27	116630.0
28	147758.0
29	183511.5
30	219963.5
31	259061.0
32	300862.0
33	343679.5
34	386204.0
35	425521.5
36	467751.0
37	505697.5
38	535493.0
39	562579.0
40	588881.0
41	612925.5
42	629777.0
43	644916.5
44	658963.5
45	666879.0
46	670100.0
47	681223.5
48	735496.0
49	747397.5
50	705176.0
51	702522.5
52	695658.5
53	649068.5
54	589224.0
55	530985.0
56	476660.0
57	429996.0
58	383335.5
59	333134.0
60	282948.5
61	235950.0
62	188649.0
63	146675.5
64	110384.5
65	81524.0
66	61807.5
67	45989.0
68	34022.5
69	25176.0
70	17708.0
71	12400.5
72	8620.5
73	5912.5
74	4089.0
75	2763.0
76	1872.5
77	1294.0
78	918.0
79	667.0
80	495.0
81	358.0
82	272.5
83	202.0
84	154.0
85	116.0
86	83.5
87	67.5
88	54.0
89	41.5
90	31.0
91	22.0
92	16.5
93	11.0
94	6.5
95	5.0
96	5.0
97	4.5
98	2.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	2.444912056513327E-4
3	2.63298221470666E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	2.5076021092444386E-5
16-17	6.269005273111096E-6
18-19	1.2224560282566636E-4
20-21	0.0
22-23	7.83788101820721E-5
24-25	0.0017719458631336473
26-27	0.002978311303140182
28-29	0.0028837231919762382
30-31	0.004384465818732784
32-33	0.002036192791020175
34-35	0.005923393142114149
36-37	0.021535689207598047
38-39	0.009426899706180067
40-41	0.003743178562255058
42-43	0.0074942999928605735
44-45	0.004419379717164244
46-47	0.0035659349051996208
48-49	0.0023104189976163136
50-51	7.966666934435194E-5
52-53	5.678707419298052E-5
54-55	0.002773822885390413
56-57	0.0026035405503816835
58-59	0.004159048173881266
60-61	0.0018460019459561976
62-63	0.0012321132250448154
64-65	3.149443017463662E-4
66-67	7.166130431098283E-5
68-69	9.42975411662263E-5
70-71	1.5742048883308476E-4
72-73	0.001027155250673544
74-75	0.002722174870052508
76-77	0.0031131124371138116
78-79	0.0011823110302966251
80-81	0.005690734953231842
82-83	0.004037048369784305
84-85	0.006639880315567974
86-87	0.003175898720709042
88-89	0.05472147762240594
90-91	0.0
92-93	1.5751511450789232E-4
94-95	0.0015060807694663681
96-97	0.023773500546340816
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	574.0
20-21	1921.0
22-23	4047.0
24-25	8864.0
26-27	15501.0
28-29	16938.0
30-31	31563.0
32-33	40770.0
34-35	56133.0
36-37	85053.0
38-39	82292.0
40-41	85886.0
42-43	97411.0
44-45	112199.0
46-47	113174.0
48-49	110925.0
50-51	97713.0
52-53	91255.0
54-55	125883.0
56-57	154607.0
58-59	145554.0
60-61	155423.0
62-63	151608.0
64-65	154567.0
66-67	192563.0
68-69	129660.0
70-71	126050.0
72-73	123714.0
74-75	126219.0
76-77	127177.0
78-79	137796.0
80-81	144595.0
82-83	141841.0
84-85	144922.0
86-87	155934.0
88-89	167639.0
90-91	182966.0
92-93	201130.0
94-95	266809.0
96-97	377643.0
98-99	1423461.0
100-101	9841514.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.7070727501923
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.25696510314555	71.29511195643703
2	8.817301301355258	14.408717577803987
3	2.1645709679192553	5.305822726461981
4	0.8083037749369066	2.6417654137209956
5	0.37026185494672503	1.5126506159376605
6	0.18751087039359285	0.9192578597220702
7	0.10735720463267197	0.6140290050425308
8	0.07312042728970175	0.47795648576678457
9	0.047970106504171675	0.35275472837737454
>10	0.16175094759771141	2.07725460355218
>50	0.003824914428545415	0.20936553154714205
>100	9.766951392623459E-4	0.12673085692454109
>500	7.153473685995426E-5	0.0430110141971029
>1k	1.4296973808980244E-5	0.015571624508642178
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.5076021092444382E-5	0.0
2	0.0	1.2538010546222191E-5	0.0	6.269005273111095E-5	0.0
3	0.0	1.2538010546222191E-5	0.0	1.0030408436977753E-4	0.0
4	0.0	6.895905800422205E-5	0.0	2.444912056513327E-4	6.2690052731110956E-6
5	0.0	6.895905800422205E-5	0.0	3.0091225310933256E-4	6.2690052731110956E-6
6	0.0	6.895905800422205E-5	0.0	3.1971926892866584E-4	6.2690052731110956E-6
7	0.0	6.895905800422205E-5	0.0	3.5106429529422137E-4	6.2690052731110956E-6
8	0.0	8.149706855044423E-5	0.0	4.200233532984434E-4	6.2690052731110956E-6
9	0.0	1.0657308964288862E-4	0.0	8.901987487817755E-4	6.2690052731110956E-6
10-11	0.0	1.0657308964288862E-4	0.0	0.0012757425730781078	6.2690052731110956E-6
12-13	0.0	1.0657308964288862E-4	0.0	0.0013948536732672188	6.2690052731110956E-6
14-15	0.0	1.4105261864499964E-4	0.0	0.0015076957681832185	9.403507909666644E-6
16-17	0.0	1.5985963446433292E-4	0.0	0.0016738244079206625	1.2538010546222191E-5
18-19	0.0	1.9433916346644396E-4	0.0	0.001836818545021551	1.2538010546222191E-5
20-21	0.0	2.3195319510511053E-4	0.0	0.002031157708487995	1.2538010546222191E-5
22-23	0.0	2.695672267437771E-4	0.0	0.0022913214273221055	1.5672513182777738E-5
24-25	9.403507909666644E-6	2.852397399265548E-4	0.0	0.002639251219979771	1.8807015819333285E-5
26-27	1.2538010546222191E-5	2.97777750472777E-4	0.0	0.0032003271919232145	1.8807015819333285E-5
28-29	1.2538010546222191E-5	3.0718125838244365E-4	0.0	0.004939976155211544	2.1941518455888835E-5
30-31	2.821052372899993E-5	3.5733330056733246E-4	0.0	0.010368934721725752	2.5076021092444382E-5
32-33	3.1345026365555476E-5	3.6360230584044355E-4	0.0	0.022527670448924723	3.1345026365555476E-5
34-35	3.761403163866658E-5	4.137543480253323E-4	0.0	0.041933376271840114	5.328654482144431E-5
36-37	4.388303691177767E-5	4.733098981198877E-4	0.0	0.06946057842607094	5.9555550094555405E-5
38-39	4.388303691177767E-5	4.795789033929988E-4	0.0	0.11104289040261683	6.58245553676665E-5
40-41	4.388303691177767E-5	5.015204218488876E-4	0.0	0.16314772773007968	7.209356064077759E-5
42-43	5.0152042184888765E-5	5.077894271219987E-4	0.0	0.20073354884501726	7.522806327733314E-5
44-45	5.328654482144431E-5	5.61075971943443E-4	0.0	0.2505219887240656	7.522806327733314E-5
46-47	6.269005273111095E-5	6.080935114917762E-4	0.0	0.30206261557694847	7.522806327733314E-5
48-49	6.895905800422205E-5	6.080935114917762E-4	0.0	0.3441871965096185	9.403507909666644E-5
50-51	6.895905800422205E-5	6.770525694959984E-4	0.0	0.3860986312630027	9.403507909666644E-5
52-53	6.895905800422205E-5	6.833215747691094E-4	0.0	0.4266590953800315	9.403507909666644E-5
54-55	6.895905800422205E-5	6.895905800422205E-4	0.0	0.5014514627908835	1.0343858700633308E-4
56-57	6.895905800422205E-5	7.021285905884427E-4	0.0	0.5562801829095131	1.2851460809877745E-4
58-59	6.895905800422205E-5	7.334736169539982E-4	0.0	0.5980568340495254	1.3478361337188855E-4
60-61	6.895905800422205E-5	7.648186433195537E-4	0.0	0.6216157558658769	1.4418712128155519E-4
62-63	6.895905800422205E-5	7.773566538657759E-4	0.0	0.6356395206618264	1.5672513182777737E-4
64-65	6.895905800422205E-5	8.964677540548866E-4	0.0	0.6460460694151908	1.5985963446433292E-4
66-67	6.895905800422205E-5	9.027367593279977E-4	0.0	0.6515972735845307	1.6299413710088847E-4
68-69	7.522806327733314E-5	9.21543775147331E-4	0.0	0.6564714251843746	1.6299413710088847E-4
70-71	7.522806327733314E-5	9.434852936032198E-4	0.0	0.6615681264714139	1.6299413710088847E-4
72-73	7.522806327733314E-5	9.56023304149442E-4	0.0	0.6654611787460158	1.7553214764711068E-4
74-75	7.522806327733314E-5	9.84233827878442E-4	0.0	0.6693416930100716	1.7553214764711068E-4
76-77	7.522806327733314E-5	0.0010406548753364418	0.0	0.6729181605183816	1.7866665028366623E-4
78-79	7.522806327733314E-5	0.0010625963937923306	0.0	0.6760401251443908	1.8180115292022178E-4
80-81	7.522806327733314E-5	0.0010657308964288862	0.0	0.6775917039494859	1.8180115292022178E-4
82-83	7.522806327733314E-5	0.0010782689069751084	0.0	0.6785790722800009	1.8180115292022178E-4
84-85	7.522806327733314E-5	0.001115882938613775	0.0	0.6795131540656945	1.8180115292022178E-4
86-87	7.522806327733314E-5	0.0011346899544331082	0.0	0.6796918207159781	1.8493565555677732E-4
88-89	7.522806327733314E-5	0.0011785729913448859	0.0	0.6797545107687092	1.8807015819333287E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	18005	0.0	52.23712	1
CTTATAC	35885	0.0	43.78998	1
TATACAC	43305	0.0	38.11644	3
TTATACA	42700	0.0	37.5232	2
GTATCAA	30265	0.0	33.31239	1
TACACAT	54195	0.0	30.338741	5
ACACATC	56900	0.0	27.902878	6
ATACACA	64015	0.0	26.902397	4
ACATCTC	58965	0.0	26.896643	8
TCAACGC	37135	0.0	26.272959	4
ATCAACG	37560	0.0	26.04409	3
CACATCT	61640	0.0	25.576544	7
CAACGCA	38510	0.0	25.346	5
TATCAAC	39880	0.0	24.81903	2
AACGCAG	39605	0.0	24.666862	6
CATCTCC	66655	0.0	23.620083	9
ACGCAGA	46515	0.0	21.039328	7
CGCAGAG	47385	0.0	20.625925	8
GTACATG	47980	0.0	19.022308	1
TACATGG	48570	0.0	18.482605	2
>>END_MODULE