FastQCFastQC Report
Fri 27 May 2016
SRR2139735_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139735_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15717327
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA154850.046.0142061
GTATCAA270150.036.677211
TCAACGC319000.030.0910954
ATCAACG323350.029.7401583
CAACGCA330250.029.0528495
AACGCAG342150.028.106046
TATCAAC353100.027.5306422
ACGCAGA395800.024.3403267
CGCAGAG403950.023.784558
CTTATAC147050.021.2656781
GTACATG448500.020.4413471
TACATGG447350.020.016842
GCAGAGT502900.018.9401869
ACATGGG460650.018.8336873
TATACAC206800.018.7437533
AGAGTAC435900.018.62913110-11
GAGTACT234900.017.43787412-13
CAGAGTA483400.017.09140410-11
CATGGGG337150.016.715844
TTATACA213350.016.1882692
Produced by FastQC (version 0.11.5)