##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139735_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15717327
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37742206419705	34.0	31.0	34.0	31.0	34.0
2	32.5024631732864	34.0	31.0	34.0	31.0	34.0
3	32.57768779640457	34.0	31.0	34.0	31.0	34.0
4	36.11092573183723	37.0	35.0	37.0	35.0	37.0
5	36.02092245074496	37.0	35.0	37.0	35.0	37.0
6	36.04073097162132	37.0	35.0	37.0	35.0	37.0
7	35.959374326181546	37.0	35.0	37.0	35.0	37.0
8	35.9316080272428	37.0	35.0	37.0	35.0	37.0
9	37.62077444847969	39.0	37.0	39.0	35.0	39.0
10-11	37.51392727274809	39.0	37.0	39.0	35.0	39.0
12-13	37.48267781156427	39.0	37.0	39.0	35.0	39.0
14-15	38.72456983302568	40.0	38.0	41.0	34.0	41.0
16-17	38.77795365586019	40.0	38.0	41.0	35.0	41.0
18-19	38.76169774287956	40.0	38.0	41.0	34.5	41.0
20-21	38.73096245249833	40.0	38.0	41.0	34.0	41.0
22-23	38.6379328745995	40.0	38.0	41.0	34.0	41.0
24-25	38.563125941588005	40.0	38.0	41.0	34.0	41.0
26-27	38.4249402784291	40.0	38.0	41.0	34.0	41.0
28-29	38.29476881163996	40.0	38.0	41.0	34.0	41.0
30-31	38.1573667646764	40.0	38.0	41.0	33.5	41.0
32-33	38.36113127548485	40.0	38.0	41.0	34.0	41.0
34-35	38.33814792311813	40.0	38.0	41.0	34.0	41.0
36-37	38.23610562203292	40.0	38.0	41.0	33.5	41.0
38-39	38.11370100954433	40.0	38.0	41.0	33.0	41.0
40-41	38.07155530369199	40.0	38.0	41.0	33.0	41.0
42-43	37.96628644739631	40.0	37.0	41.0	33.0	41.0
44-45	37.997309974636146	40.0	37.5	41.0	33.0	41.0
46-47	38.121613551813	40.0	38.0	41.0	33.0	41.0
48-49	38.054599304346745	40.0	37.0	41.0	33.0	41.0
50-51	37.950229656183865	40.0	37.0	41.0	33.0	41.0
52-53	37.80876875637419	40.0	37.0	41.0	33.0	41.0
54-55	37.70101025724037	40.0	37.0	41.0	32.5	41.0
56-57	37.55275700024835	39.5	36.0	41.0	32.0	41.0
58-59	37.404409407287694	39.0	36.0	41.0	32.0	41.0
60-61	37.16710910787691	39.0	35.5	40.5	31.5	41.0
62-63	36.92630266902431	39.0	35.0	40.0	31.0	41.0
64-65	36.688621731900554	38.0	35.0	40.0	31.0	41.0
66-67	36.400545304795955	38.0	35.0	40.0	31.0	41.0
68-69	36.0811826923528	37.0	34.5	40.0	30.5	41.0
70-71	35.768191436149046	37.0	34.0	39.0	30.0	41.0
72-73	35.43401044685932	36.0	34.0	39.0	30.0	40.0
74-75	35.023281638673055	36.0	34.0	38.5	29.5	40.0
76-77	34.00647370090143	35.0	32.5	37.0	28.5	39.0
78-79	34.288187566505606	35.0	33.5	37.0	29.0	39.0
80-81	34.15444911374759	35.0	33.5	36.5	29.0	39.0
82-83	33.89531174399542	35.0	33.0	36.0	29.0	37.5
84-85	33.64339063397729	35.0	33.0	36.0	29.0	37.0
86-87	33.42609913091779	35.0	33.0	35.0	29.0	37.0
88-89	33.18897851614998	35.0	33.0	35.0	29.0	36.0
90-91	33.06544040164946	35.0	33.0	35.0	29.0	36.0
92-93	32.97768982910834	34.0	33.0	35.0	29.0	36.0
94-95	32.929058605293335	34.0	32.0	35.0	29.0	35.5
96-97	32.885465176764946	34.0	32.0	35.0	29.0	35.0
98-99	32.98353778594321	34.0	32.0	35.0	29.0	35.0
100-101	32.172058328280016	33.5	30.5	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	10.0
20	67.0
21	484.0
22	2314.0
23	8248.0
24	21809.0
25	46104.0
26	83989.0
27	131957.0
28	188177.0
29	250633.0
30	318411.0
31	394553.0
32	495158.0
33	635101.0
34	860871.0
35	1226694.0
36	1962959.0
37	3432374.0
38	4094819.0
39	1511245.0
40	51349.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	38.158409505636676	21.786242660727233	15.53227212235261	24.523075711283475
2	13.677758426822246	22.787698102782777	40.26585892739858	23.268684542996397
3	16.36326491372966	27.857143720614747	31.877877843868525	23.901713521787073
4	11.976311239182081	20.18740845692146	39.8357621496327	28.000518154263766
5	12.73318293880378	38.63395474306795	36.05235164987023	12.580510668258032
6	28.126716457575768	39.49039808104775	19.714153685292672	12.668731776083808
7	25.582301621643428	33.18479662604207	23.912482065175585	17.320419687138912
8	24.903732040441735	36.12973121956424	21.636535270914706	17.330001469079313
9	25.670637252759327	16.768201106969396	21.05186842520996	36.50929321506131
10-11	23.77194290097801	26.93200631379623	29.686899050964584	19.609151734261175
12-13	24.99060749960855	24.367833029114937	29.268052385752362	21.37350708552415
14-15	22.156319701165366	25.777156114036355	26.630377192449746	25.436146992348537
16-17	21.903116465429072	28.945090847202053	27.078259182144542	22.07353350522433
18-19	21.960478219298114	28.193062705140093	28.440528747034797	21.405930328527
20-21	22.84007973859748	27.303260701126636	28.16055783550448	21.696101724771403
22-23	22.436470960908018	27.404672237896776	28.03788178544252	22.120975015752684
24-25	22.250173270331185	27.592446540159543	28.101392533982082	22.055987655527186
26-27	22.279323697714034	27.625118243765424	27.978669211528146	22.1168888469924
28-29	22.140216630570915	27.47690977042872	28.041657167365365	22.341216431635004
30-31	22.444660640220906	27.31777988066295	28.020818116960992	22.216741362155147
32-33	22.265872680415875	27.542183910235806	28.001300354968055	22.19064305438026
34-35	22.35025323332975	27.640025711407983	28.06152184478713	21.948199210475135
36-37	22.37531661509863	27.521036502168727	27.870932092280796	22.232714790451844
38-39	22.249467531753332	27.510819187784364	28.035775327473324	22.20393795298898
40-41	22.478138055529065	27.358690098488665	27.973995009142516	22.189176836839756
42-43	22.387106914358494	27.4338235341873	27.877842501407734	22.30122705004647
44-45	22.38681419519909	27.52026503539493	27.79501556462483	22.297905204781152
46-47	22.51253601599293	27.456804557008173	27.49946315187193	22.531196275126966
48-49	22.34745973722589	27.54352780404195	27.64300243781181	22.466010020920347
50-51	22.36107684149505	27.619485917016682	27.597552622672172	22.421884618816097
52-53	22.49540745976562	27.56173480767721	27.573656129765904	22.369201602791264
54-55	22.38327539425127	27.522820390777824	27.60103823581738	22.492865979153525
56-57	22.482715949018274	27.66179338832544	27.459807396885726	22.395683265770554
58-59	22.349927947263108	27.735017466108285	27.622704587605888	22.29234999902271
60-61	22.475508433927434	27.790909119081814	27.45847254441297	22.275109902577782
62-63	22.36245922905734	27.93476427048503	27.416593211840436	22.2861832886172
64-65	22.327692311728608	27.829945542904966	27.44027516268478	22.402086982681645
66-67	22.414372424931063	27.802687465971097	27.35328279511083	22.42965731398701
68-69	22.376866352190287	27.755666775396463	27.411690736007703	22.455776136405543
70-71	22.36244352950943	27.536895585858268	27.490999622835865	22.609661261796433
72-73	22.351375532692042	27.61661258653823	27.42665344842153	22.6053584323482
74-75	22.255777945830513	27.69835111318856	27.42683154547956	22.61903939550137
76-77	22.354559425660952	27.644015507820885	27.404781313864135	22.596643752654025
78-79	22.327525343088077	27.676504486431902	27.357667946959257	22.638302223520764
80-81	22.334123668095486	27.727004490925683	27.336529252660053	22.602342588318784
82-83	22.327380058641026	27.67727860312923	27.452329621458	22.543011716771748
84-85	22.36787671008358	27.698024394731995	27.469548807158912	22.464550088025515
86-87	22.214355858070217	27.727565310071277	27.508833654915748	22.549245176942755
88-89	22.290114134589707	27.70021710424472	27.516940757616865	22.49272800354871
90-91	22.255338411092985	27.753763723849858	27.573614237242484	22.417283627814676
92-93	22.262086841090404	27.69171439140142	27.58072272526112	22.465476042247058
94-95	22.09146873192112	27.768370607225478	27.645375199007532	22.494785461845872
96-97	22.010375243786022	27.84855430432434	27.749568265804296	22.391502186085344
98-99	22.18588608905522	28.45650193315226	27.794458494484253	21.563153483308266
100-101	21.656217739225127	31.66711638513086	25.353746737031546	21.322919138612466
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	618.0
1	741.5
2	1682.0
3	2638.0
4	3406.5
5	5030.0
6	6392.5
7	7361.0
8	7953.5
9	7881.0
10	7468.5
11	7235.0
12	8507.5
13	12351.5
14	21494.0
15	33744.0
16	43420.5
17	48724.5
18	49518.0
19	46891.0
20	42489.0
21	38162.0
22	36025.5
23	38078.0
24	44761.0
25	57162.0
26	75285.0
27	97531.0
28	126807.0
29	163292.5
30	202939.5
31	248389.5
32	299934.5
33	353412.0
34	405359.0
35	452593.5
36	502277.5
37	547387.5
38	581649.0
39	610776.0
40	637304.0
41	662099.0
42	676216.5
43	685407.0
44	694718.5
45	700594.5
46	707259.0
47	711969.0
48	701814.5
49	681934.0
50	660831.0
51	628318.5
52	586075.0
53	543353.5
54	501011.0
55	457163.5
56	414402.5
57	370982.0
58	325988.5
59	279184.5
60	230947.0
61	186274.0
62	145984.0
63	111166.5
64	82883.5
65	62232.5
66	47201.0
67	36146.5
68	27837.0
69	20293.0
70	14285.5
71	10349.5
72	7479.0
73	5212.5
74	3604.0
75	2469.0
76	1698.0
77	1147.5
78	780.0
79	548.0
80	399.0
81	304.5
82	226.0
83	150.5
84	99.5
85	72.5
86	53.0
87	41.0
88	31.0
89	22.0
90	17.0
91	15.5
92	13.0
93	8.0
94	4.0
95	1.5
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	2.799458203039232E-4
3	3.0539544033155255E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	3.49932275379904E-5
16-17	1.2724810013814692E-5
18-19	1.2088569513123955E-4
20-21	0.0
22-23	1.3045025542000925E-4
24-25	0.002033565801622569
26-27	0.0031835223937083153
28-29	0.0030415949091790904
30-31	0.004640595592396728
32-33	0.0022593268795086615
34-35	0.006360956741594475
36-37	0.02290093976546425
38-39	0.010206027585940174
40-41	0.003932631470099721
42-43	0.0074391640522707934
44-45	0.0042646804358372785
46-47	0.003536679358502899
48-49	0.002259908839975888
50-51	9.65783361204509E-5
52-53	3.350567199093015E-5
54-55	0.0026185391086061506
56-57	0.0025751863659658754
58-59	0.004165257888063905
60-61	0.001880024416903196
62-63	0.0011730884757558187
64-65	2.483664136407592E-4
66-67	6.351057901007119E-5
68-69	1.245811271053899E-4
70-71	1.867145690074785E-4
72-73	0.0010832332127563137
74-75	0.0029274967600176308
76-77	0.0033219722619371814
78-79	0.0011386092267831253
80-81	0.00557387978453027
82-83	0.003980499956111949
84-85	0.006509464631077244
86-87	0.0030805779878192237
88-89	0.05287819688528615
90-91	0.0
92-93	1.6144216284065557E-4
94-95	0.001424430902909394
96-97	0.023939789936014973
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	437.0
20-21	1486.0
22-23	2734.0
24-25	5244.0
26-27	6380.0
28-29	9318.0
30-31	18088.0
32-33	23726.0
34-35	34970.0
36-37	58354.0
38-39	66090.0
40-41	72760.0
42-43	82692.0
44-45	97468.0
46-47	101518.0
48-49	98472.0
50-51	93276.0
52-53	84452.0
54-55	96506.0
56-57	103547.0
58-59	109344.0
60-61	117043.0
62-63	111711.0
64-65	117040.0
66-67	127861.0
68-69	120660.0
70-71	124535.0
72-73	121077.0
74-75	120052.0
76-77	120414.0
78-79	132448.0
80-81	138974.0
82-83	137206.0
84-85	138294.0
86-87	148503.0
88-89	160114.0
90-91	178786.0
92-93	198488.0
94-95	264424.0
96-97	376676.0
98-99	1464510.0
100-101	1.0131649E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.12142395963048
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.8870209782852	72.17407310306696
2	8.198911281858507	13.466125387697996
3	2.1207898387358632	5.224868444283127
4	0.7994873076655584	2.6262014457258744
5	0.3793582493170275	1.5576719812373412
6	0.20340375078614226	1.0022283391972686
7	0.1182785301326719	0.6799240922842955
8	0.07574796398926747	0.49764245318731654
9	0.05209349226260345	0.385019258727149
>10	0.1602295665183466	2.0199723066836093
>50	0.0036956022206523775	0.20275570301119253
>100	9.268789310781725E-4	0.12878528822353963
>500	4.243391373437287E-5	0.02278423173973638
>1k	1.4125383361087972E-5	0.011947964934558622
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.272481001381469E-5	0.0
2	0.0	1.9087215020722034E-5	0.0	1.9087215020722034E-5	0.0
3	0.0	1.9087215020722034E-5	0.0	2.544962002762938E-5	0.0
4	0.0	6.99864550759808E-5	0.0	6.362405006907345E-5	0.0
5	0.0	7.634886008288814E-5	0.0	6.99864550759808E-5	0.0
6	0.0	7.634886008288814E-5	0.0	6.99864550759808E-5	0.0
7	0.0	7.634886008288814E-5	0.0	9.543607510361018E-5	0.0
8	0.0	8.271126508979548E-5	0.0	1.0179848011051752E-4	0.0
9	0.0	1.7814734019340567E-4	0.0	2.290465802486644E-4	0.0
10-11	0.0	1.7814734019340567E-4	0.0	3.085766428350062E-4	0.0
12-13	0.0	1.7814734019340567E-4	0.0	3.467510728764503E-4	0.0
14-15	0.0	2.4813379526938647E-4	0.0	3.722006929040797E-4	0.0
16-17	0.0	2.640398077866548E-4	0.0	3.9765031293170907E-4	0.0
18-19	0.0	3.022142378280989E-4	0.0	4.1037512294552376E-4	9.543607510361017E-6
20-21	0.0	3.308450603591819E-4	0.0	4.3264354046969946E-4	2.226841752417571E-5
22-23	0.0	4.040127179386164E-4	0.0	4.803615780215046E-4	4.771803755180508E-5
24-25	0.0	4.4218714798006047E-4	0.0	5.72616450621661E-4	5.72616450621661E-5
26-27	0.0	4.580931604973288E-4	0.0	6.648713232218175E-4	5.72616450621661E-5
28-29	0.0	4.580931604973288E-4	0.0	0.0010148035986017215	5.72616450621661E-5
30-31	0.0	5.217172105664023E-4	0.0	0.0022841033974797366	5.72616450621661E-5
32-33	0.0	5.217172105664023E-4	0.0	0.005271252548222735	6.362405006907345E-5
34-35	0.0	5.694352481182074E-4	0.0	0.00971857364805097	6.362405006907345E-5
36-37	0.0	6.266968931803735E-4	0.0	0.015673784734516243	6.362405006907345E-5
38-39	0.0	6.744149307321785E-4	0.0	0.02655667849883126	6.680525257252712E-5
40-41	6.362405006907345E-6	7.09408158270169E-4	0.0	0.045239880801614674	6.99864550759808E-5
42-43	6.362405006907345E-6	7.221329682839837E-4	0.0	0.05639953918373016	6.99864550759808E-5
44-45	6.362405006907345E-6	7.921194233599645E-4	0.0	0.06989738140588408	7.316765757943447E-5
46-47	6.362405006907345E-6	8.461998659186769E-4	0.0	0.08542483082524147	8.271126508979548E-5
48-49	6.362405006907345E-6	8.557434734290379E-4	0.0	0.0993139609553202	1.0179848011051752E-4
50-51	9.543607510361017E-6	9.257299285050187E-4	0.0	0.11527087271264383	1.0179848011051752E-4
52-53	1.272481001381469E-5	9.352735360153797E-4	0.0	0.13215033319596903	1.1134208762087854E-4
54-55	1.272481001381469E-5	9.41635941022287E-4	0.0	0.18093089238392762	1.272481001381469E-4
56-57	1.272481001381469E-5	9.543607510361017E-4	0.0	0.21289561513863015	1.3679170764850792E-4
58-59	1.272481001381469E-5	9.639043585464627E-4	0.0	0.24588786630194814	1.399729101519616E-4
60-61	1.9087215020722034E-5	9.798103710637312E-4	0.0	0.2669728764948391	1.4633531515886893E-4
62-63	1.9087215020722034E-5	9.957163835809997E-4	0.0	0.2774104019086706	1.5269772016577628E-4
64-65	1.9087215020722034E-5	0.0011197832812156927	0.0	0.2877334040323778	1.5587892266922995E-4
66-67	1.9087215020722034E-5	0.0011388704962364148	0.0	0.2936345346762843	1.5906012517268362E-4
68-69	1.9087215020722034E-5	0.0011579577112571368	0.0	0.2985208617215892	1.5906012517268362E-4
70-71	1.9087215020722034E-5	0.0011738637237744052	0.0	0.30341991357690784	1.5906012517268362E-4
72-73	1.9087215020722034E-5	0.0011834073312847661	0.0	0.30771135575406683	1.8450974520031302E-4
74-75	1.9087215020722034E-5	0.0012215817613262102	0.0	0.3113315642029971	1.8450974520031302E-4
76-77	1.9087215020722034E-5	0.0012692997988780152	0.0	0.31460502157905096	1.9087215020722036E-4
78-79	1.9087215020722034E-5	0.001291568216402191	0.0	0.3175699023122698	1.9087215020722036E-4
80-81	1.9087215020722034E-5	0.001291568216402191	0.0	0.3192654832466106	1.9087215020722036E-4
82-83	1.9087215020722034E-5	0.0012947494189056445	0.0	0.32034391089528136	2.0995936522794239E-4
84-85	1.9087215020722034E-5	0.0013042930264160057	0.0	0.32122510398873805	2.0995936522794239E-4
86-87	1.9087215020722034E-5	0.0013520110639678108	0.0	0.3213555332913796	2.0995936522794239E-4
88-89	1.9087215020722034E-5	0.0014219975190437918	0.0	0.32138416411391074	2.0995936522794239E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15485	0.0	46.014206	1
GTATCAA	27015	0.0	36.67721	1
TCAACGC	31900	0.0	30.091095	4
ATCAACG	32335	0.0	29.740158	3
CAACGCA	33025	0.0	29.052849	5
AACGCAG	34215	0.0	28.10604	6
TATCAAC	35310	0.0	27.530642	2
ACGCAGA	39580	0.0	24.340326	7
CGCAGAG	40395	0.0	23.78455	8
CTTATAC	14705	0.0	21.265678	1
GTACATG	44850	0.0	20.441347	1
TACATGG	44735	0.0	20.01684	2
GCAGAGT	50290	0.0	18.940186	9
ACATGGG	46065	0.0	18.833687	3
TATACAC	20680	0.0	18.743753	3
AGAGTAC	43590	0.0	18.629131	10-11
GAGTACT	23490	0.0	17.437874	12-13
CAGAGTA	48340	0.0	17.091404	10-11
CATGGGG	33715	0.0	16.71584	4
TTATACA	21335	0.0	16.188269	2
>>END_MODULE