FastQCFastQC Report
Fri 27 May 2016
SRR2139734_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139734_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7115727
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC95150.054.934321
GGTATCA77300.048.2442471
TATACAC128050.042.9310723
TTATACA127350.042.0553132
GTATCAA137650.036.694881
TACACAT171500.031.929255
ACACATC173550.029.7470026
ACATCTC175750.029.5943958
ATCAACG167050.029.491553
TCAACGC167600.029.3947724
CAACGCA172550.028.5266425
TATCAAC177750.027.9095922
AACGCAG178300.027.6307536
CACATCT192000.026.9779057
ATACACA223800.025.9250034
CATCTCC203750.025.1904419
ACGCAGA211550.023.2473687
CGCAGAG216300.022.8162128
GTACATG240550.020.2664761
TACATGG240100.019.8576182
Produced by FastQC (version 0.11.5)