##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139734_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7115727
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37681083043236	34.0	31.0	34.0	31.0	34.0
2	32.50773715742608	34.0	31.0	34.0	31.0	34.0
3	32.57514249773776	34.0	31.0	34.0	31.0	34.0
4	36.10969125150529	37.0	35.0	37.0	35.0	37.0
5	36.01924680359435	37.0	35.0	37.0	35.0	37.0
6	36.03760192036597	37.0	35.0	37.0	35.0	37.0
7	35.95592312633691	37.0	35.0	37.0	35.0	37.0
8	35.930711507060344	37.0	35.0	37.0	35.0	37.0
9	37.61325905279953	39.0	37.0	39.0	35.0	39.0
10-11	37.51215778795336	39.0	37.0	39.0	35.0	39.0
12-13	37.47533273831331	39.0	37.0	39.0	35.0	39.0
14-15	38.708243725483	40.0	38.0	41.0	34.0	41.0
16-17	38.75281106203203	40.0	38.0	41.0	34.5	41.0
18-19	38.73736738354352	40.0	38.0	41.0	34.0	41.0
20-21	38.711766373484444	40.0	38.0	41.0	34.0	41.0
22-23	38.61609476690525	40.0	38.0	41.0	34.0	41.0
24-25	38.52837631275497	40.0	38.0	41.0	34.0	41.0
26-27	38.38313616782759	40.0	38.0	41.0	34.0	41.0
28-29	38.249374466585756	40.0	38.0	41.0	34.0	41.0
30-31	38.10411363486271	40.0	38.0	41.0	33.0	41.0
32-33	38.3052212171165	40.0	38.0	41.0	34.0	41.0
34-35	38.2820116751399	40.0	38.0	41.0	34.0	41.0
36-37	38.17469137943225	40.0	38.0	41.0	33.5	41.0
38-39	38.03225460894067	40.0	37.5	41.0	33.0	41.0
40-41	38.01136464932179	40.0	37.0	41.0	33.0	41.0
42-43	37.91062390669421	40.0	37.0	41.0	33.0	41.0
44-45	37.94396992337017	40.0	37.0	41.0	33.0	41.0
46-47	38.06374598074838	40.0	37.5	41.0	33.0	41.0
48-49	38.002465262040936	40.0	37.0	41.0	33.0	41.0
50-51	37.881503014236856	40.0	37.0	41.0	33.0	41.0
52-53	37.75433493751619	40.0	37.0	41.0	33.0	41.0
54-55	37.640689661153964	40.0	36.5	41.0	32.0	41.0
56-57	37.48090599793805	39.0	36.0	41.0	32.0	41.0
58-59	37.346954321826615	39.0	36.0	41.0	32.0	41.0
60-61	37.0939850197916	39.0	35.0	40.5	31.0	41.0
62-63	36.86797274237291	38.5	35.0	40.0	31.0	41.0
64-65	36.645042999496376	38.0	35.0	40.0	31.0	41.0
66-67	36.36329415665428	38.0	35.0	40.0	31.0	41.0
68-69	36.05244065419514	37.0	34.5	40.0	30.5	41.0
70-71	35.740776987696975	36.5	34.0	39.0	30.0	41.0
72-73	35.410700212928994	36.0	34.0	39.0	30.0	40.0
74-75	35.00920248236703	36.0	34.0	38.5	29.5	40.0
76-77	34.00392846648457	35.0	32.5	37.0	28.5	39.0
78-79	34.28394631332073	35.0	33.5	37.0	29.0	39.0
80-81	34.15688867029559	35.0	33.5	36.5	29.0	39.0
82-83	33.900513966079814	35.0	33.0	36.0	29.0	37.5
84-85	33.64748290639455	35.0	33.0	36.0	29.0	37.0
86-87	33.43080053545943	35.0	33.0	35.0	29.0	37.0
88-89	33.19720416544753	35.0	33.0	35.0	29.0	36.0
90-91	33.074455534795774	35.0	33.0	35.0	29.0	36.0
92-93	32.986469881755895	34.0	33.0	35.0	29.0	36.0
94-95	32.93755074925717	34.0	32.5	35.0	29.0	35.5
96-97	32.89567444214924	34.0	32.0	35.0	29.0	35.0
98-99	32.991597512567225	34.0	32.0	35.0	29.0	35.0
100-101	32.182835936846935	33.5	30.5	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	8.0
20	39.0
21	226.0
22	1226.0
23	4041.0
24	10491.0
25	21626.0
26	38617.0
27	60083.0
28	86722.0
29	114722.0
30	145500.0
31	180558.0
32	225965.0
33	290433.0
34	392138.0
35	556353.0
36	882863.0
37	1532167.0
38	1827113.0
39	719593.0
40	25243.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	37.75834851449472	21.999508974978944	15.529895961438655	24.712246549087677
2	13.759053025591946	22.66064618945531	40.38115635603731	23.199144428915435
3	16.239255461297738	27.953353341649205	32.33695086982209	23.470440327230964
4	11.945511681378445	20.62681718958583	38.899370366513494	28.52830076252223
5	12.603195710009674	38.6152672804901	36.10404109095248	12.677495918547747
6	27.941586291885567	39.3054427186428	19.482436580267905	13.270534409203727
7	25.253203221540115	33.17766406721337	23.416665647796776	18.152467063449738
8	24.868829284765983	35.93562541114913	21.716263145002614	17.479282159082267
9	25.3814684009097	16.577828238773073	20.802863853545812	37.23783950677141
10-11	23.673455994025627	26.885060374013786	29.57153218497562	19.869951446984967
12-13	24.9670483423549	24.304670485531553	28.94902376102962	21.779257411083925
14-15	22.19848293743786	25.765540405229263	26.15541634900168	25.880560308331198
16-17	22.027840584665487	29.108157184782385	26.683787896865635	22.180214333686497
18-19	22.11943259997354	27.94003783594367	28.186323507226007	21.754206056856788
20-21	22.919425971003413	27.045586143701193	27.867910368050907	22.167077517244483
22-23	22.48383114603572	27.595736658433335	27.662242875250065	22.258189320280884
24-25	22.486611091036146	27.814806326567055	27.862309008566932	21.836273573829867
26-27	22.63904740979266	27.780962262287805	27.867660732225442	21.712329595694097
28-29	22.224470941013518	27.297423374066653	28.204593752491547	22.273511932428285
30-31	22.74978953999698	27.47136250735342	27.83709663241456	21.94175132023504
32-33	22.527769937563363	27.219014847528033	28.003989420895543	22.24922579401306
34-35	22.119146062728365	27.670615104232034	28.486087370008256	21.724151463031347
36-37	22.621866703004002	27.453313262631664	27.878509800080764	22.04631023428357
38-39	22.13560139688172	27.187194457078412	28.249892925175818	22.427311220864045
40-41	22.34819338044586	27.051195711230374	28.322600469309904	22.278010439013862
42-43	22.528770872355206	27.007326941079395	28.208957386006762	22.254944800558636
44-45	22.217083905558628	27.407268300095044	28.089142191049497	22.28650560329683
46-47	22.71393781262838	26.998036874232177	27.523372869632606	22.764652443506833
48-49	22.394314526303035	27.14195396363786	27.645287097212424	22.818444412846688
50-51	22.259198924045304	27.240858179612616	27.91984220677643	22.580100689565654
52-53	22.332076040519095	27.331815091072855	28.13148681816489	22.20462205024316
54-55	22.26621669726537	27.383802638141958	27.760703733503707	22.58927693108897
56-57	22.638344369778995	27.722421835255357	27.20951111249576	22.429722682469887
58-59	22.140361715614944	27.95966472383511	27.880568876301894	22.019404684248055
60-61	22.602220616207006	28.26497721376549	27.126080213405885	22.006721956621618
62-63	22.22144305974649	28.54823539809474	27.134773727038297	22.09554781512047
64-65	22.268228086238008	28.267798545598527	27.23759816275122	22.226375205412243
66-67	22.417867871674495	27.97566001424213	27.25862751182806	22.347844602255314
68-69	22.439860863794472	27.732622706435233	27.397882827081204	22.42963360268909
70-71	22.422794231229243	27.543947218348592	27.48012620302573	22.55313234739644
72-73	22.408536345758968	27.636943059288516	27.42846166108904	22.52605893386347
74-75	22.281822268108673	27.75085818553912	27.443078968102775	22.524240578249433
76-77	22.419133465545034	27.656828253155357	27.435490721472323	22.488547559827285
78-79	22.351794195010186	27.727620442410274	27.343890209082765	22.57669515349678
80-81	22.358976911462218	27.749732575007734	27.356599322012414	22.534691191517627
82-83	22.37614816783387	27.704667452560333	27.47657949214597	22.44260488745983
84-85	22.397739450342666	27.68939103757926	27.489770202693958	22.423099309384117
86-87	22.271059171062603	27.759340812545908	27.493807534474474	22.47579248191702
88-89	22.32135934484594	27.758282010458846	27.460693130264048	22.459665514431165
90-91	22.295918554022904	27.793823863012694	27.507674435622555	22.402583147341844
92-93	22.300815177936347	27.734387228702495	27.5437768099606	22.421020783400554
94-95	22.1086637742353	27.864334316911272	27.587214936518095	22.439786972335334
96-97	22.038905623195408	27.90502494704392	27.687395508844006	22.368673920916667
98-99	22.234938259333596	28.48612183868425	27.740850608652355	21.538089293329797
100-101	21.608928874935945	31.71199294469284	25.33577763455117	21.343300545820046
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	583.0
1	614.0
2	1018.0
3	1455.0
4	1839.0
5	2639.0
6	3312.0
7	3862.0
8	4292.0
9	4367.0
10	4168.5
11	4044.0
12	4771.5
13	6920.5
14	11777.5
15	18239.5
16	23346.5
17	26015.5
18	26335.0
19	24833.5
20	22312.0
21	19743.5
22	18236.0
23	18885.0
24	21914.0
25	27723.0
26	36362.5
27	46700.5
28	60128.5
29	76682.0
30	94441.0
31	114625.0
32	136532.5
33	158526.0
34	181011.5
35	201913.0
36	222651.5
37	241398.5
38	256227.5
39	269233.5
40	281412.5
41	291570.0
42	297309.5
43	303172.0
44	308651.5
45	313704.5
46	329128.5
47	347280.0
48	342460.5
49	330113.0
50	322546.5
51	303763.0
52	279267.5
53	255901.5
54	234167.5
55	213340.0
56	193182.0
57	173918.0
58	153653.5
59	131237.5
60	108714.5
61	88418.5
62	69995.5
63	54113.5
64	40922.5
65	30909.0
66	23760.0
67	18289.0
68	14216.5
69	10504.5
70	7460.0
71	5506.5
72	3957.0
73	2757.0
74	1958.5
75	1405.5
76	987.0
77	683.5
78	486.0
79	340.0
80	232.0
81	160.0
82	122.0
83	91.0
84	63.0
85	41.0
86	31.0
87	26.5
88	20.0
89	13.5
90	9.5
91	6.0
92	3.5
93	5.0
94	4.5
95	3.0
96	3.0
97	2.5
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.6864053384847396E-4
3	2.670141785934171E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	2.8106755641412325E-5
16-17	0.0
18-19	9.134695583459006E-5
20-21	0.0
22-23	1.1947631143173243E-4
24-25	0.0021089986755488317
26-27	0.0031648476294658286
28-29	0.00333543615929452
30-31	0.005014725826908568
32-33	0.002391394366326346
34-35	0.006356221008137659
36-37	0.02207431743634795
38-39	0.008883194898793247
40-41	0.003453850254811027
42-43	0.007343482569137915
44-45	0.004244796623086513
46-47	0.00362756625629092
48-49	0.0021081108533138877
50-51	8.168266892031625E-5
52-53	5.233940308406194E-5
54-55	0.0028169998559865314
56-57	0.0026214749709928095
58-59	0.004388479902680088
60-61	0.001953433100536303
62-63	0.0010890744598641196
64-65	3.010419616844882E-4
66-67	4.011413916702027E-5
68-69	7.305181167275418E-5
70-71	1.3111644918954873E-4
72-73	9.099934571470431E-4
74-75	0.002622385214691504
76-77	0.003178848599114307
78-79	0.001107286807469825
80-81	0.0057864819171579
82-83	0.004223027761139629
84-85	0.006929453496807389
86-87	0.003465255336760462
88-89	0.05393764667314147
90-91	0.0
92-93	1.5792056149863267E-4
94-95	0.001493780766683924
96-97	0.02416443988543304
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	252.0
20-21	787.0
22-23	1467.0
24-25	3021.0
26-27	3561.0
28-29	5242.0
30-31	10395.0
32-33	14380.0
34-35	21490.0
36-37	33130.0
38-39	35209.0
40-41	38058.0
42-43	45183.0
44-45	53329.0
46-47	53914.0
48-49	51096.0
50-51	46711.0
52-53	47228.0
54-55	54344.0
56-57	63407.0
58-59	66463.0
60-61	69885.0
62-63	68862.0
64-65	71833.0
66-67	81734.0
68-69	58680.0
70-71	58095.0
72-73	56784.0
74-75	57301.0
76-77	58068.0
78-79	63092.0
80-81	64446.0
82-83	63695.0
84-85	64894.0
86-87	69165.0
88-89	74372.0
90-91	82239.0
92-93	89642.0
94-95	118705.0
96-97	168389.0
98-99	635948.0
100-101	4391231.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.45569236638111
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.12298602030376	77.91645154511046
2	7.140287364028608	12.346369755040312
3	1.630681196754417	4.229450156827262
4	0.5561120959622058	1.9231622515892748
5	0.2324374793970608	1.004777160658467
6	0.11399883396285616	0.5913508871531311
7	0.059061505947419844	0.35743423722197226
8	0.03738330930825589	0.2585599911353474
9	0.02649681062866048	0.20617200975615557
>10	0.07921165838270118	1.0305331621697067
>50	0.0010975846853269338	0.06715001707587938
>100	1.9809682435121875E-4	0.040323233596145654
>500	4.80438143497065E-5	0.028265592665830834
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.4053377820706162E-5	1.4053377820706162E-5
2	0.0	1.4053377820706162E-5	0.0	5.621351128282465E-5	1.4053377820706162E-5
3	0.0	1.4053377820706162E-5	0.0	5.621351128282465E-5	1.4053377820706162E-5
4	0.0	4.216013346211849E-5	0.0	8.432026692423698E-5	1.4053377820706162E-5
5	0.0	4.216013346211849E-5	0.0	8.432026692423698E-5	1.4053377820706162E-5
6	1.4053377820706162E-5	4.216013346211849E-5	0.0	8.432026692423698E-5	1.4053377820706162E-5
7	1.4053377820706162E-5	5.621351128282465E-5	0.0	8.432026692423698E-5	1.4053377820706162E-5
8	1.4053377820706162E-5	8.432026692423698E-5	0.0	1.124270225656493E-4	1.4053377820706162E-5
9	2.8106755641412325E-5	1.6864053384847396E-4	0.0	1.826939116691801E-4	1.4053377820706162E-5
10-11	2.8106755641412325E-5	1.6864053384847396E-4	0.0	1.9674728948988626E-4	1.4053377820706162E-5
12-13	4.216013346211849E-5	1.6864053384847396E-4	0.0	2.1080066731059245E-4	2.1080066731059245E-5
14-15	4.216013346211849E-5	1.9674728948988626E-4	0.0	2.178273562209455E-4	5.621351128282465E-5
16-17	4.216013346211849E-5	2.1080066731059245E-4	0.0	2.3890742295200475E-4	5.621351128282465E-5
18-19	4.216013346211849E-5	2.178273562209455E-4	0.0	2.740408675037701E-4	6.324020019317773E-5
20-21	4.216013346211849E-5	2.3890742295200475E-4	0.0	3.021476231451825E-4	7.026688910353081E-5
22-23	5.621351128282465E-5	2.8106755641412324E-4	0.0	3.5133444551765404E-4	7.026688910353081E-5
24-25	9.837364474494313E-5	3.0917431205553555E-4	0.0	4.145746457108318E-4	7.026688910353081E-5
26-27	9.837364474494313E-5	3.2322768987624173E-4	0.0	5.691618017385995E-4	7.026688910353081E-5
28-29	9.837364474494313E-5	3.2322768987624173E-4	0.0	8.221226025113105E-4	7.026688910353081E-5
30-31	1.1945371147600238E-4	4.1457464571083183E-4	0.0	0.0015739783159190901	7.729357801388389E-5
32-33	1.2648040038635547E-4	4.216013346211849E-4	0.0	0.0029793160979897067	8.432026692423698E-5
34-35	1.4053377820706162E-4	4.567347791729503E-4	0.0	0.005255963304944105	8.432026692423698E-5
36-37	1.5458715602776778E-4	4.918682237247157E-4	0.0	0.008565533781720406	8.432026692423698E-5
38-39	1.5458715602776778E-4	5.410550460971872E-4	0.0	0.0132242285292845	8.432026692423698E-5
40-41	1.5458715602776778E-4	6.253753130214242E-4	0.0	0.01780562969883471	8.432026692423698E-5
42-43	1.5458715602776778E-4	6.464553797524835E-4	0.0	0.02214812344543291	8.432026692423698E-5
44-45	1.6864053384847396E-4	7.378023355870735E-4	0.0	0.027214366149797484	8.432026692423698E-5
46-47	1.897206005795332E-4	8.010425357802513E-4	0.0	0.03274437032224536	9.837364474494313E-5
48-49	1.9674728948988626E-4	8.080692246906044E-4	0.0	0.037880879915713456	1.2648040038635547E-4
50-51	1.9674728948988626E-4	9.415763139873129E-4	0.0	0.04324224355431286	1.2648040038635547E-4
52-53	2.1080066731059245E-4	9.415763139873129E-4	0.0	0.04784472479059413	1.6864053384847396E-4
54-55	2.1080066731059245E-4	9.48603002897666E-4	0.0	0.053297435385028125	2.178273562209455E-4
56-57	2.1080066731059245E-4	9.696830696287252E-4	0.0	0.05905932029151765	2.318807340416517E-4
58-59	2.1080066731059245E-4	9.907631363597845E-4	0.0	0.0642028565738961	2.3890742295200475E-4
60-61	2.3890742295200475E-4	0.0010118432030908437	0.0	0.06936747292300562	2.3890742295200475E-4
62-63	2.5296080077271093E-4	0.0010258965809115499	0.0	0.07399103422601794	2.5296080077271093E-4
64-65	2.5296080077271093E-4	0.0011523769812979052	0.0	0.07875512930723734	2.59987489683064E-4
66-67	2.5296080077271093E-4	0.0011734570480289646	0.0	0.0838213720116019	2.8106755641412324E-4
68-69	2.5296080077271093E-4	0.0012015638036703769	0.0	0.08906328193872531	2.8106755641412324E-4
70-71	2.5296080077271093E-4	0.001215617181491083	0.0	0.09413655133200022	3.021476231451825E-4
72-73	2.5296080077271093E-4	0.0012366972482221422	0.0	0.09838067143385348	3.2322768987624173E-4
74-75	2.5296080077271093E-4	0.0013069641373256733	0.0	0.10220319020108556	3.2322768987624173E-4
76-77	2.5296080077271093E-4	0.001377231026429204	0.0	0.10554086743350329	3.2322768987624173E-4
78-79	2.5296080077271093E-4	0.0014193911598913225	0.0	0.10906123857759017	3.2322768987624173E-4
80-81	2.5296080077271093E-4	0.0014193911598913225	0.0	0.11086007093864056	3.3025437878659485E-4
82-83	2.6701417859341706E-4	0.0014404712266223817	0.0	0.11205460805340059	3.372810676969479E-4
84-85	2.8106755641412324E-4	0.0014545246044430878	0.0	0.11314374483450532	3.372810676969479E-4
86-87	2.8106755641412324E-4	0.0015177648046362656	0.0	0.11349507928002298	3.372810676969479E-4
88-89	2.8106755641412324E-4	0.0015880316937397965	0.0	0.11357939954694721	3.372810676969479E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	9515	0.0	54.93432	1
GGTATCA	7730	0.0	48.244247	1
TATACAC	12805	0.0	42.931072	3
TTATACA	12735	0.0	42.055313	2
GTATCAA	13765	0.0	36.69488	1
TACACAT	17150	0.0	31.92925	5
ACACATC	17355	0.0	29.747002	6
ACATCTC	17575	0.0	29.594395	8
ATCAACG	16705	0.0	29.49155	3
TCAACGC	16760	0.0	29.394772	4
CAACGCA	17255	0.0	28.526642	5
TATCAAC	17775	0.0	27.909592	2
AACGCAG	17830	0.0	27.630753	6
CACATCT	19200	0.0	26.977905	7
ATACACA	22380	0.0	25.925003	4
CATCTCC	20375	0.0	25.190441	9
ACGCAGA	21155	0.0	23.247368	7
CGCAGAG	21630	0.0	22.816212	8
GTACATG	24055	0.0	20.266476	1
TACATGG	24010	0.0	19.857618	2
>>END_MODULE