FastQCFastQC Report
Fri 27 May 2016
SRR2139733_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139733_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22568076
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA244150.048.1725921
CTTATAC211900.039.9703181
GTATCAA416650.035.6541371
TATACAC293100.032.3863333
TTATACA293550.030.1701742
TCAACGC512000.028.4098364
ATCAACG520000.028.0224673
CAACGCA531700.027.3814685
TATCAAC544300.027.0082632
AACGCAG545050.026.7029276
TACACAT375450.024.5959915
ACACATC373300.022.9534076
ACATCTC387100.022.457058
ACGCAGA653700.022.2844127
CGCAGAG661450.022.0363068
GTACATG715450.019.9707451
ATACACA523650.019.875274
CACATCT429500.019.8098877
TACATGG714800.019.6041562
ACATGGG743250.018.3912263
Produced by FastQC (version 0.11.5)