##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139733_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22568076
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.38266576202597	34.0	31.0	34.0	31.0	34.0
2	32.50915713860588	34.0	31.0	34.0	31.0	34.0
3	32.58152963504731	34.0	31.0	34.0	31.0	34.0
4	36.11420264625128	37.0	35.0	37.0	35.0	37.0
5	36.02043457315546	37.0	35.0	37.0	35.0	37.0
6	36.03995249750134	37.0	35.0	37.0	35.0	37.0
7	35.956452778695	37.0	35.0	37.0	35.0	37.0
8	35.93034067237278	37.0	35.0	37.0	35.0	37.0
9	37.61440598658034	39.0	37.0	39.0	35.0	39.0
10-11	37.512345647010406	39.0	37.0	39.0	35.0	39.0
12-13	37.47808029802806	39.0	37.0	39.0	35.0	39.0
14-15	38.71436415315156	40.0	38.0	41.0	34.0	41.0
16-17	38.76354094163809	40.0	38.0	41.0	35.0	41.0
18-19	38.74348907722572	40.0	38.0	41.0	34.0	41.0
20-21	38.7072277551219	40.0	38.0	41.0	34.0	41.0
22-23	38.611533306294575	40.0	38.0	41.0	34.0	41.0
24-25	38.53213885024269	40.0	38.0	41.0	34.0	41.0
26-27	38.38247046264466	40.0	38.0	41.0	34.0	41.0
28-29	38.24488459411349	40.0	38.0	41.0	34.0	41.0
30-31	38.09917462010017	40.0	38.0	41.0	33.0	41.0
32-33	38.31458225861388	40.0	38.0	41.0	34.0	41.0
34-35	38.30361411162258	40.0	38.0	41.0	34.0	41.0
36-37	38.196380557011686	40.0	38.0	41.0	33.5	41.0
38-39	38.06857049246564	40.0	38.0	41.0	33.0	41.0
40-41	38.02708056643532	40.0	37.5	41.0	33.0	41.0
42-43	37.92046891724462	40.0	37.0	41.0	33.0	41.0
44-45	37.946247755243846	40.0	37.0	41.0	33.0	41.0
46-47	38.05971769388696	40.0	37.0	41.0	33.0	41.0
48-49	37.98925739755414	40.0	37.0	41.0	33.0	41.0
50-51	37.864972516213456	40.0	37.0	41.0	33.0	41.0
52-53	37.72306244522961	40.0	37.0	41.0	32.5	41.0
54-55	37.60571279662209	40.0	36.0	41.0	32.0	41.0
56-57	37.448341877473666	39.0	36.0	41.0	32.0	41.0
58-59	37.29740317847711	39.0	36.0	41.0	32.0	41.0
60-61	37.04859048965556	39.0	35.0	40.5	31.0	41.0
62-63	36.80306219750524	38.5	35.0	40.0	31.0	41.0
64-65	36.55946479553252	38.0	35.0	40.0	31.0	41.0
66-67	36.265841381448524	37.5	35.0	40.0	31.0	41.0
68-69	35.94306818176196	37.0	34.5	39.5	30.0	41.0
70-71	35.631709365281395	36.5	34.0	39.0	30.0	41.0
72-73	35.30014762668331	36.0	34.0	39.0	30.0	40.0
74-75	34.897575291097766	35.5	34.0	38.0	29.5	40.0
76-77	33.898933322316246	35.0	32.5	37.0	28.5	39.0
78-79	34.18628575624395	35.0	33.0	37.0	29.0	39.0
80-81	34.067910728849746	35.0	33.0	36.5	29.0	39.0
82-83	33.82641547933393	35.0	33.0	36.0	29.0	37.5
84-85	33.58932626688872	35.0	33.0	35.5	29.0	37.0
86-87	33.38910008080411	35.0	33.0	35.0	29.0	37.0
88-89	33.16752206654607	35.0	33.0	35.0	29.0	36.0
90-91	33.05336487238453	35.0	33.0	35.0	29.0	36.0
92-93	32.972108407921276	34.0	33.0	35.0	29.0	36.0
94-95	32.929123707956336	34.0	32.5	35.0	29.0	35.0
96-97	32.891981091732006	34.0	32.0	35.0	29.0	35.0
98-99	32.99065382527489	34.0	32.0	35.0	29.0	35.0
100-101	32.18685784877576	33.5	30.5	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	5.0
19	31.0
20	132.0
21	798.0
22	3712.0
23	12464.0
24	32166.0
25	68769.0
26	122573.0
27	193047.0
28	275458.0
29	366014.0
30	464312.0
31	576970.0
32	722322.0
33	927413.0
34	1261327.0
35	1795994.0
36	2872339.0
37	4941721.0
38	5673917.0
39	2176621.0
40	79970.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	38.514098410515814	21.47191457526109	15.416183461984087	24.59780355223901
2	14.117205361620194	22.472662543990882	39.713078177983554	23.697053916405373
3	16.64947638887984	27.372598418051336	31.865661579328137	24.112263613740684
4	12.386372679709162	19.999680079063893	38.664944233615664	28.949003007611285
5	13.100385695262636	38.21978887345116	35.616195195372434	13.063630235913775
6	28.806252690747762	38.64601483972316	19.25801738703822	13.289715082490861
7	25.91373318664825	33.02032038530888	23.211003897718175	17.854942530324696
8	25.367434955465413	35.49400046330932	21.3221632185216	17.816401362703672
9	25.69216356768738	16.564176760127893	20.689597110537914	37.054062561646816
10-11	24.068090695901592	26.587456990130658	29.250364541487716	20.09408777248003
12-13	25.288679903417556	24.09400783655638	28.743026211007088	21.874286049018977
14-15	22.575014883305382	25.55075651900518	26.0098121365842	25.86441646110524
16-17	22.341064143509453	28.72881778195123	26.534687307949113	22.39543076659021
18-19	22.51872198542608	27.737058835714777	27.924972702008738	21.819246476850406
20-21	23.297641599972277	26.894564398542308	27.616755735919803	22.191038265565613
22-23	22.868057043003226	27.081306582010782	27.569517376468937	22.48111899851705
24-25	22.837463493454823	27.199305720631823	27.55168548321814	22.411545302695217
26-27	22.83279483673069	27.266270131806092	27.542698018223838	22.358237013239375
28-29	22.641898528203306	27.022854269129017	27.649311152374327	22.68593605029335
30-31	22.875723565915486	27.002693950846137	27.66990064848581	22.451681834752566
32-33	22.53219140001267	27.024674081651685	27.81695439243182	22.626180125903826
34-35	22.616300601215276	27.069282023873786	27.67705095897957	22.637366415931368
36-37	22.69335838076679	27.127422952940993	27.732377028878265	22.446841637413957
38-39	22.596945195030653	27.023087193409317	27.77447156250664	22.605496049053382
40-41	22.765209551245388	26.88709951869098	27.76781618216001	22.57987474790362
42-43	22.752956303991535	26.951515021049175	27.680587666240346	22.614941008718933
44-45	22.68775376328146	27.114119052242703	27.526632486853497	22.67149469762234
46-47	22.969902103769225	26.969464429246337	27.125831125490325	22.934802341494116
48-49	22.720571099242008	27.060857650240163	27.309616656184126	22.908954594333704
50-51	22.689255165953565	27.192433207413067	27.32850352019589	22.789808106437473
52-53	22.794035214199624	27.173050099239177	27.383239154568926	22.649675531992273
54-55	22.71589441975595	27.13065636186086	27.26193557384265	22.891513644540535
56-57	22.877568158014387	27.355800694357846	27.03008601362511	22.736545134002654
58-59	22.662620274683004	27.465117595910705	27.299863500247483	22.572398629158805
60-61	22.885873501391057	27.56905792601223	26.987521347720495	22.557547224876217
62-63	22.718079322590206	27.765129478364713	26.93907251057733	22.577718688467755
64-65	22.74516910472295	27.61253018078792	26.939865336827275	22.70243537766186
66-67	22.81874982190328	27.47819688164097	26.954236643248073	22.74881665320768
68-69	22.75583272079043	27.39505881832458	27.043920543816334	22.805187917068658
70-71	22.783268217205794	27.185096412003663	27.100802869673775	22.93083250111677
72-73	22.76579691543567	27.2807566324712	27.011872525307318	22.941573926785818
74-75	22.57378306975147	27.383585279046457	27.09080338051657	22.9518282706855
76-77	22.745727909759196	27.31933093480037	27.061824357993437	22.873116797447
78-79	22.701945268535226	27.352799299780884	27.013851720761206	22.931403710922684
80-81	22.699074971575982	27.382582566991374	27.021992506415852	22.896349955016788
82-83	22.688326712745656	27.359726664535682	27.117680023414874	22.834266599303792
84-85	22.68471498156595	27.37878660761262	27.131244715431073	22.805253695390356
86-87	22.588857201700797	27.363823821463733	27.184954243087272	22.86236473374819
88-89	22.629685054112926	27.370487212586426	27.12656039035069	22.87326734294996
90-91	22.618001592457414	27.447521693067657	27.18979925064397	22.744677463830957
92-93	22.619924406899784	27.389253887374608	27.21778620007193	22.77303550565367
94-95	22.455729093317725	27.443799374809615	27.28284788020127	22.817623651671394
96-97	22.383133826957252	27.509127119102857	27.36754550366457	22.74019355027532
98-99	22.568577980305886	28.110226420341444	27.405976347844508	21.915219251508166
100-101	22.02773640674941	31.269451697487533	24.94068286727666	21.762129028486395
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1671.0
1	1728.0
2	2928.0
3	4253.0
4	5343.5
5	7728.0
6	9802.5
7	11480.0
8	12671.5
9	12848.0
10	12234.0
11	11762.0
12	13756.0
13	19599.5
14	33551.0
15	52486.5
16	67779.5
17	76368.0
18	78015.5
19	74002.0
20	66692.0
21	58592.0
22	53265.5
23	54265.0
24	62521.5
25	78878.5
26	102661.5
27	131656.5
28	170068.0
29	219295.5
30	271325.0
31	331804.5
32	401954.0
33	472256.5
34	539777.0
35	602203.5
36	667166.0
37	728904.5
38	777375.5
39	814870.0
40	854929.0
41	895435.5
42	920588.0
43	941280.5
44	961041.5
45	975293.5
46	984383.0
47	991506.0
48	1019331.0
49	1021176.5
50	987427.5
51	963857.0
52	931564.0
53	879105.0
54	816079.5
55	749496.5
56	686383.0
57	624280.5
58	555868.5
59	481923.0
60	405644.5
61	333865.5
62	266437.0
63	206547.0
64	155560.5
65	117101.5
66	89945.0
67	69664.0
68	53693.0
69	39508.0
70	28482.0
71	21031.0
72	15369.5
73	10935.0
74	7731.5
75	5496.0
76	3801.5
77	2569.0
78	1788.0
79	1242.5
80	876.5
81	615.5
82	467.0
83	349.0
84	262.0
85	207.5
86	164.0
87	125.5
88	86.5
89	58.5
90	39.5
91	29.0
92	24.5
93	17.0
94	11.0
95	8.5
96	8.0
97	7.5
98	3.5
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	2.0825878112073E-4
3	3.8550029696815974E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.9939670532835853E-5
16-17	6.646556844278617E-6
18-19	1.04129390560365E-4
20-21	0.0
22-23	1.0636485992213293E-4
24-25	0.0019616660312124783
26-27	0.003013671691271223
28-29	0.003048607382169494
30-31	0.004703876118292135
32-33	0.002275752761952991
34-35	0.00603150120408163
36-37	0.022818095179162667
38-39	0.009820597672534093
40-41	0.0037565537963140474
42-43	0.007244056373351255
44-45	0.004189880630506784
46-47	0.0033696296959168665
48-49	0.0021663493150872793
50-51	1.1005502774803365E-4
52-53	4.9511699119384916E-5
54-55	0.002562487833229397
56-57	0.0025605053522585897
58-59	0.004308857348063842
60-61	0.0019122547749721266
62-63	0.001260369337941526
64-65	2.7332812701230067E-4
66-67	5.021226607831281E-5
68-69	1.2683782297792987E-4
70-71	1.7672644835776564E-4
72-73	0.0010602573197967264
74-75	0.0029450689806693295
76-77	0.003451089662446785
78-79	0.0011984801992694384
80-81	0.005625109324767154
82-83	0.004052284160653351
84-85	0.006477816645289689
86-87	0.003082229536587625
88-89	0.05075054638693086
90-91	2.8622786846764675E-6
92-93	1.598750207546845E-4
94-95	0.001369818959341732
96-97	0.02389350410517993
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	797.0
20-21	2411.0
22-23	4846.0
24-25	10052.0
26-27	11413.0
28-29	17579.0
30-31	34486.0
32-33	47257.0
34-35	69646.0
36-37	107495.0
38-39	112386.0
40-41	119509.0
42-43	139952.0
44-45	167887.0
46-47	170196.0
48-49	161792.0
50-51	147055.0
52-53	148499.0
54-55	171644.0
56-57	186674.0
58-59	189680.0
60-61	191375.0
62-63	186264.0
64-65	193762.0
66-67	218206.0
68-69	188234.0
70-71	188378.0
72-73	183926.0
74-75	185782.0
76-77	186502.0
78-79	203606.0
80-81	209409.0
82-83	205196.0
84-85	209867.0
86-87	223681.0
88-89	239706.0
90-91	263079.0
92-93	287450.0
94-95	379908.0
96-97	536620.0
98-99	2036087.0
100-101	1.4029782E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.51100774406369
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.74239903989964	68.96975561800124
2	8.7404491590152	13.89923841538105
3	2.309492880917029	5.5089031901836165
4	0.9264846513055237	2.9466291313883857
5	0.4452774764770818	1.7702230440213196
6	0.25032894328505667	1.1942343928860855
7	0.15980670690761883	0.8894474617339501
8	0.1020884768625151	0.6493726139516085
9	0.06941036320316689	0.49669991335487484
>10	0.24579246375481126	3.0596136490971757
>50	0.006544192525889668	0.3442775866530968
>100	0.0019004482284148473	0.2487864063316509
>500	1.2600883612560536E-5	0.007915080740552735
>1k	1.2596734454597904E-5	0.014903496275449384
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.431037896185745E-6
2	0.0	1.3293113688557235E-5	0.0	3.101726527330021E-5	4.431037896185745E-6
3	0.0	1.3293113688557235E-5	0.0	4.431037896185745E-5	4.431037896185745E-6
4	0.0	3.544830316948596E-5	0.0	1.2406906109320085E-4	4.431037896185745E-6
5	0.0	3.9879341065671706E-5	0.0	1.5065528847031532E-4	4.431037896185745E-6
6	0.0	3.9879341065671706E-5	0.0	1.5065528847031532E-4	4.431037896185745E-6
7	0.0	4.431037896185745E-5	0.0	1.5951736426268682E-4	8.86207579237149E-6
8	0.0	4.8741416858043194E-5	0.0	1.7281047795124406E-4	8.86207579237149E-6
9	0.0	7.975868213134341E-5	0.0	3.9879341065671706E-4	8.86207579237149E-6
10-11	0.0	8.197420107943629E-5	0.0	5.405866233346608E-4	8.86207579237149E-6
12-13	0.0	8.418972002752915E-5	0.0	5.871125212446112E-4	1.9939670532835853E-5
14-15	0.0	1.3957769372985096E-4	0.0	6.42500494946933E-4	2.6586227377114467E-5
16-17	0.0	1.528708074184082E-4	0.0	6.868108739087904E-4	3.544830316948596E-5
18-19	0.0	1.7502599689933694E-4	0.0	7.444143665592052E-4	4.874141685804319E-5
20-21	0.0	2.0825878112073E-4	0.0	8.241730486905485E-4	7.089660633897192E-5
22-23	0.0	2.5478467903068035E-4	0.0	9.127938066142634E-4	7.089660633897192E-5
24-25	0.0	2.7693986851160907E-4	0.0	0.0010723111708769502	7.975868213134341E-5
26-27	0.0	2.8358642535588767E-4	0.0	0.0012495526867243802	7.975868213134341E-5
28-29	0.0	2.8358642535588767E-4	0.0	0.0018167255374361554	7.975868213134341E-5
30-31	0.0	3.3897439905820946E-4	0.0	0.0033609422442568877	7.975868213134341E-5
32-33	0.0	3.4118991800630237E-4	0.0	0.006812720765385583	7.975868213134341E-5
34-35	0.0	4.0543996750099567E-4	0.0	0.012378104362994878	9.748283371608639E-5
36-37	0.0	4.6969001699568897E-4	0.0	0.019786799725417442	9.748283371608639E-5
38-39	0.0	4.8741416858043193E-4	0.0	0.02908754826951132	9.969835266417925E-5
40-41	2.2155189480928724E-6	5.405866233346609E-4	0.0	0.03880259885689857	1.1077594740464362E-4
42-43	4.431037896185745E-6	5.583107749194038E-4	0.0	0.04741875204603175	1.1520698530082936E-4
44-45	4.431037896185745E-6	6.535780896873974E-4	0.0	0.059528778616307385	1.1520698530082936E-4
46-47	4.431037896185745E-6	7.355522907668337E-4	0.0	0.06802750930119164	1.2628458004129372E-4
48-49	4.431037896185745E-6	7.421988476111123E-4	0.0	0.07464304888019696	1.285000989893866E-4
50-51	4.431037896185745E-6	8.773455034447775E-4	0.0	0.08074901910114092	1.3293113688557235E-4
52-53	4.431037896185745E-6	8.817765413409632E-4	0.0	0.08589566961756066	1.440087316260367E-4
54-55	6.646556844278618E-6	8.884230981852418E-4	0.0	0.09128602721827062	1.5730184531459395E-4
56-57	8.86207579237149E-6	9.172248445104492E-4	0.0	0.09649471226523697	1.683794400550583E-4
58-59	8.86207579237149E-6	9.238714013547278E-4	0.0	0.10119604347309004	1.7945703479552266E-4
60-61	1.1077594740464362E-5	9.460265908356565E-4	0.0	0.10595719369254161	1.861035916398013E-4
62-63	1.3293113688557235E-5	9.659662613684923E-4	0.0	0.11057211966141908	1.9496566743217278E-4
64-65	1.3293113688557235E-5	0.0010634490950845788	0.0	0.11515159732712704	1.9496566743217278E-4
66-67	1.3293113688557235E-5	0.0010656646140326715	0.0	0.1200789114676856	1.9496566743217278E-4
68-69	1.3293113688557235E-5	0.0010856042845655074	0.0	0.12512143259354497	1.9718118638026565E-4
70-71	1.772415158474298E-5	0.0011033284361502505	0.0	0.13017503131414482	2.0604326217263713E-4
72-73	1.772415158474298E-5	0.0011166215498388077	0.0	0.13443104321343122	2.3041397060165873E-4
74-75	1.772415158474298E-5	0.001163147447748758	0.0	0.1382262271715143	2.3041397060165873E-4
76-77	1.772415158474298E-5	0.0012606302814648445	0.0	0.14178656612109955	2.3041397060165873E-4
78-79	1.772415158474298E-5	0.0012916475467381447	0.0	0.145136430770616	2.3041397060165873E-4
80-81	1.772415158474298E-5	0.0012982941035824232	0.0	0.14695094078910403	2.414915653421231E-4
82-83	1.772415158474298E-5	0.0013071561793747948	0.0	0.14806091578209857	2.570001979787732E-4
84-85	1.772415158474298E-5	0.0013182337741152591	0.0	0.14906011482768844	2.6143123587495895E-4
86-87	1.772415158474298E-5	0.0013536820772847452	0.0	0.14919526148352213	2.658622737711447E-4
88-89	2.4370708429021597E-5	0.00142679420257181	0.0	0.14923735634353588	2.7029331166733046E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	24415	0.0	48.172592	1
CTTATAC	21190	0.0	39.970318	1
GTATCAA	41665	0.0	35.654137	1
TATACAC	29310	0.0	32.386333	3
TTATACA	29355	0.0	30.170174	2
TCAACGC	51200	0.0	28.409836	4
ATCAACG	52000	0.0	28.022467	3
CAACGCA	53170	0.0	27.381468	5
TATCAAC	54430	0.0	27.008263	2
AACGCAG	54505	0.0	26.702927	6
TACACAT	37545	0.0	24.595991	5
ACACATC	37330	0.0	22.953407	6
ACATCTC	38710	0.0	22.45705	8
ACGCAGA	65370	0.0	22.284412	7
CGCAGAG	66145	0.0	22.036306	8
GTACATG	71545	0.0	19.970745	1
ATACACA	52365	0.0	19.87527	4
CACATCT	42950	0.0	19.809887	7
TACATGG	71480	0.0	19.604156	2
ACATGGG	74325	0.0	18.391226	3
>>END_MODULE