FastQCFastQC Report
Fri 27 May 2016
SRR2139732_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139732_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6775955
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68700.046.0114751
GTATCAA121400.033.6545221
TCAACGC146050.026.942434
ATCAACG148500.026.5527863
CAACGCA150550.026.1912255
AACGCAG155000.025.4655636
TATCAAC157150.025.2986092
GTACATG211550.023.0292261
TACATGG210950.022.8243892
ACATGGG216100.022.1484973
ACGCAGA186250.021.1928187
CGCAGAG191000.020.601798
CATGGGG140950.019.0161274
GCAGAGT226400.017.2725329
AGAGTAC201950.016.79196710-11
GAGTACT118550.016.25252212-13
ATGGGGG82800.016.0871325
CAGAGTA222850.015.68324210-11
GTGGTAT36800.015.607471
GAGTACA147950.015.4457531
Produced by FastQC (version 0.11.5)