FastQCFastQC Report
Fri 27 May 2016
SRR2139731_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139731_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8660769
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91150.043.9798471
GTATCAA146600.032.365151
TCAACGC168300.027.2880384
ATCAACG171900.026.7645513
CAACGCA174400.026.4043085
AACGCAG180350.025.669986
TATCAAC183100.025.3968622
ACGCAGA214400.021.5164877
CGCAGAG218750.020.9758428
GTACATG210800.020.4212281
TACATGG210400.020.069222
ACATGGG213550.019.1185723
TATACCG5750.017.876215
GCAGAGT264050.017.2059789
GAGTACT148150.016.74861512-13
CATGGGG165300.016.6401234
CAGAGTA255550.016.52333810-11
AGAGTAC230300.016.38898310-11
GTACTTT169950.014.49181314-15
TATACTG46300.014.2971075
Produced by FastQC (version 0.11.5)