##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139731_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8660769
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.386561516650545	34.0	31.0	34.0	31.0	34.0
2	32.50531713754287	34.0	31.0	34.0	31.0	34.0
3	32.58283427256864	34.0	31.0	34.0	31.0	34.0
4	36.11330252544549	37.0	35.0	37.0	35.0	37.0
5	36.01897083272859	37.0	35.0	37.0	35.0	37.0
6	36.03934304217097	37.0	35.0	37.0	35.0	37.0
7	35.951780725245065	37.0	35.0	37.0	35.0	37.0
8	35.929022007168186	37.0	35.0	37.0	35.0	37.0
9	37.618684206910494	39.0	37.0	39.0	35.0	39.0
10-11	37.520434675027126	39.0	37.0	39.0	35.0	39.0
12-13	37.48061603998444	39.0	37.0	39.0	35.0	39.0
14-15	38.727614256886426	40.0	38.0	41.0	34.0	41.0
16-17	38.779786587080196	40.0	38.0	41.0	35.0	41.0
18-19	38.76014976268274	40.0	38.0	41.0	34.0	41.0
20-21	38.72849197470087	40.0	38.0	41.0	34.0	41.0
22-23	38.63534998123369	40.0	38.0	41.0	34.0	41.0
24-25	38.56215227621252	40.0	38.0	41.0	34.0	41.0
26-27	38.42384239352928	40.0	38.0	41.0	34.0	41.0
28-29	38.289864807412386	40.0	38.0	41.0	34.0	41.0
30-31	38.14760299924204	40.0	38.0	41.0	33.5	41.0
32-33	38.36084173636934	40.0	38.0	41.0	34.0	41.0
34-35	38.34196026375309	40.0	38.0	41.0	34.0	41.0
36-37	38.24542163077182	40.0	38.0	41.0	34.0	41.0
38-39	38.13330565363336	40.0	38.0	41.0	33.0	41.0
40-41	38.095063371718666	40.0	38.0	41.0	33.0	41.0
42-43	37.99397267214299	40.0	37.0	41.0	33.0	41.0
44-45	38.01949010632744	40.0	37.5	41.0	33.0	41.0
46-47	38.126348067037334	40.0	38.0	41.0	33.0	41.0
48-49	38.05835571245576	40.0	37.0	41.0	33.0	41.0
50-51	37.95538626911129	40.0	37.0	41.0	33.0	41.0
52-53	37.81005150111514	40.0	37.0	41.0	33.0	41.0
54-55	37.70003183080979	40.0	36.5	41.0	33.0	41.0
56-57	37.55450002869564	39.0	36.0	41.0	32.0	41.0
58-59	37.3971032301673	39.0	36.0	41.0	32.0	41.0
60-61	37.15658448663061	39.0	35.0	40.5	31.5	41.0
62-63	36.90615839139176	38.5	35.0	40.0	31.0	41.0
64-65	36.658331900761134	38.0	35.0	40.0	31.0	41.0
66-67	36.3663012790844	37.5	35.0	40.0	31.0	41.0
68-69	36.043326280425276	37.0	34.5	40.0	31.0	41.0
70-71	35.73782653049611	36.5	34.0	39.0	30.0	41.0
72-73	35.41125488984623	36.0	34.0	39.0	30.0	40.5
74-75	35.01666258315558	35.5	34.0	38.5	30.0	40.0
76-77	34.02430557049688	35.0	32.5	37.0	28.5	39.0
78-79	34.307419152402055	35.0	33.5	37.0	29.0	39.0
80-81	34.18954053013505	35.0	34.0	36.5	29.0	39.0
82-83	33.94977431615294	35.0	33.0	36.0	29.0	37.5
84-85	33.71245333991683	35.0	33.0	36.0	29.0	37.0
86-87	33.507844366424955	35.0	33.0	35.0	29.0	37.0
88-89	33.2805873378651	35.0	33.0	35.0	29.0	36.0
90-91	33.16247451457887	35.0	33.0	35.0	29.0	36.0
92-93	33.075449569026574	35.0	33.0	35.0	29.0	36.0
94-95	33.02508239872037	34.0	33.0	35.0	29.0	35.5
96-97	32.98355672125459	34.0	33.0	35.0	29.0	35.0
98-99	33.07315310381763	34.0	33.0	35.0	29.0	35.0
100-101	32.218003458935584	33.5	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	12.0
20	52.0
21	297.0
22	1313.0
23	4196.0
24	11060.0
25	23548.0
26	42754.0
27	67531.0
28	97700.0
29	130638.0
30	166778.0
31	209819.0
32	263145.0
33	338110.0
34	457803.0
35	652954.0
36	1036933.0
37	1784354.0
38	2152519.0
39	1168448.0
40	50804.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.110121861003336	20.782138399026692	13.799513645959152	25.30822609401082
2	14.703998939583265	22.18829648161948	39.58600102115915	23.52170355763811
3	17.55738165068824	26.35702334408184	30.91061859732755	25.17497640790237
4	12.437521425637838	18.279739362636274	38.37109614631218	30.911643065413706
5	12.918367872414102	38.0052625811865	35.99116891352258	13.085200632876825
6	30.53833903201898	38.11035717498065	18.333579847239893	13.017723945760476
7	26.46902370909558	32.416994380060245	23.490442938727497	17.623538972116677
8	24.781517668927552	36.66495434758738	21.21041445626826	17.34311352721681
9	25.726329844382178	15.95175901816571	20.914678592628437	37.40723254482368
10-11	24.34144704702319	26.590133047076996	29.20611322158575	19.86230668431406
12-13	24.957806864494366	24.163096833549076	29.1638941068628	21.71520219509376
14-15	22.05985140689931	25.610617213917173	26.41290195422102	25.916629424962494
16-17	21.937601614821965	28.586780227021414	27.216243730781876	22.259374427374752
18-19	21.970354454397572	27.94737506626871	28.60679167761403	21.47547880171969
20-21	22.67040854820054	27.258174006613363	28.38380993652796	21.68760750865814
22-23	22.375527465141683	27.222854166714782	28.251795171456486	22.149823196687045
24-25	22.17395781719622	27.325499764151996	28.348468478063303	22.15207394058848
26-27	22.178886075291107	27.45976226141999	28.267934759841857	22.09341690344705
28-29	22.13920341844582	27.4861625208436	28.277732186242204	22.096901874468376
30-31	22.386156000782023	27.400177298885986	28.14900681120518	22.064659889126812
32-33	22.15302559308603	27.604359654794063	28.13511064194467	22.10750411017523
34-35	22.27813284630223	27.559887632612202	28.157101822797415	22.00487769828815
36-37	22.256756475945465	27.513706311882974	28.11854035864463	22.110996853526927
38-39	22.1156884690764	27.55728071287769	28.222021969471207	22.105008848574702
40-41	22.323076928590893	27.41602588279793	28.116851069615155	22.144046118996023
42-43	22.202905101014327	27.567534421711155	27.92495609933982	22.3046043779347
44-45	22.28965260137674	27.636320679759663	27.7923104498151	22.281716269048495
46-47	22.397032714953465	27.58017620492712	27.61232570256035	22.410465377559056
48-49	22.28331182487603	27.69754246029499	27.618778127105294	22.40036758772369
50-51	22.29061906184231	27.785551886648367	27.546063518646914	22.37776553286241
52-53	22.448028003548394	27.696826424352857	27.414527639917846	22.440617932180903
54-55	22.386988342644845	27.608278415005255	27.47693740597369	22.527795836376207
56-57	22.41970460480988	27.687439720678427	27.441929556304913	22.450926118206784
58-59	22.375351547904838	27.754048581989277	27.450213058833945	22.420386811271946
60-61	22.450009234850647	27.759524489144855	27.41279110767836	22.377675168326142
62-63	22.416012733630616	27.828546989842977	27.36202530337136	22.393414973155043
64-65	22.423193420973277	27.76397111004691	27.351639097545586	22.461196371434227
66-67	22.47403958318217	27.751385578817526	27.243535360864445	22.531039477135856
68-69	22.445519232882273	27.71279229757856	27.313707576022683	22.52798089351649
70-71	22.428048632051315	27.52273311852799	27.38372637122129	22.6654918781994
72-73	22.439054922804722	27.58394390614731	27.306378608760077	22.67062256228789
74-75	22.30507302984481	27.68581793153176	27.342662848322956	22.66644619030047
76-77	22.484121534230823	27.583226461742587	27.306598711824133	22.626053292202457
78-79	22.42096556675883	27.619448679071102	27.243499380897035	22.716086373273036
80-81	22.452324977956454	27.610433474074224	27.281235990211915	22.656005557757403
82-83	22.42412167951678	27.585361861936086	27.39283147680246	22.597684981744674
84-85	22.495979898737918	27.57350721247495	27.347126167575592	22.583386721211536
86-87	22.43586298460634	27.53589514211493	27.414061907123717	22.61417996615501
88-89	22.40434839464198	27.503464520432242	27.396292613601315	22.69589447132446
90-91	22.42574990138384	27.531321094883815	27.44468453879449	22.598244464937853
92-93	22.494245082846646	27.427069023233702	27.463353409840174	22.615332484079477
94-95	22.33554976183773	27.43729509898222	27.55983885975167	22.66731627942838
96-97	22.32675019060021	27.460706584715	27.611976167754733	22.600567056930053
98-99	22.492901295066133	28.076526754821096	27.619435745749747	21.81113620436302
100-101	25.19238397951529	35.90366403893281	28.941557534766293	9.96239444678561
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1507.0
1	1434.5
2	2050.5
3	2901.0
4	3879.5
5	5892.0
6	7525.5
7	8759.5
8	9652.5
9	9716.0
10	9218.5
11	8905.5
12	10191.0
13	13418.5
14	20317.5
15	29264.0
16	35997.0
17	39318.0
18	39997.5
19	39259.0
20	37682.5
21	36490.5
22	37348.5
23	41763.0
24	50016.0
25	62313.0
26	78051.0
27	94987.5
28	113501.5
29	134089.0
30	153162.0
31	171661.5
32	191268.5
33	210350.0
34	227786.0
35	244220.0
36	263350.5
37	281172.0
38	295673.5
39	308452.0
40	321064.5
41	334289.0
42	344342.5
43	352934.0
44	361392.0
45	369979.0
46	377224.5
47	381080.5
48	381496.0
49	378384.5
50	373922.0
51	366231.0
52	352765.0
53	336028.0
54	318541.5
55	298162.5
56	276059.5
57	252631.5
58	226368.5
59	197285.0
60	166438.5
61	137053.0
62	110627.5
63	87241.5
64	66522.0
65	50054.5
66	37937.5
67	29254.5
68	22382.0
69	16345.0
70	11771.5
71	8576.5
72	6151.5
73	4362.5
74	3104.0
75	2180.0
76	1516.0
77	1039.5
78	744.0
79	549.5
80	397.5
81	282.0
82	202.5
83	132.0
84	81.5
85	57.5
86	49.0
87	45.0
88	35.5
89	27.5
90	19.5
91	13.0
92	11.5
93	7.0
94	3.0
95	3.0
96	3.0
97	3.0
98	1.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	2.655653325934452E-4
3	3.463895642523199E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	4.041211582943732E-5
16-17	0.0
18-19	8.659739106307997E-5
20-21	0.0
22-23	8.083750425984777E-5
24-25	0.002004104776214846
26-27	0.0030218378922062467
28-29	0.0030181437271265036
30-31	0.004731066656328196
32-33	0.0022243353085004977
34-35	0.006333211606595825
36-37	0.023034703997011182
38-39	0.00976107273538229
40-41	0.003912468463562882
42-43	0.007608627629061196
44-45	0.004431227291483937
46-47	0.0036975493519442906
48-49	0.0023564363057454233
50-51	1.3280649208726272E-4
52-53	4.475450936846145E-5
54-55	0.002773913445494702
56-57	0.0027888433174142317
58-59	0.004614708015953364
60-61	0.0020618477769352036
62-63	0.001380464053532084
64-65	2.891520478505332E-4
66-67	7.674398437045968E-5
68-69	1.9103050912885276E-4
70-71	2.7989302345108816E-4
72-73	0.001259097250561008
74-75	0.003453125044962566
76-77	0.0039731531571976925
78-79	0.0014730386471430833
80-81	0.006369914508189775
82-83	0.004394905356770739
84-85	0.007095781677116637
86-87	0.003547787239441696
88-89	0.0536763799268423
90-91	0.0
92-93	1.9636217722967118E-4
94-95	0.0015001573420840187
96-97	0.02490498809796177
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	272.0
20-21	829.0
22-23	1642.0
24-25	3308.0
26-27	5062.0
28-29	10010.0
30-31	23164.0
32-33	32521.0
34-35	46613.0
36-37	71386.0
38-39	76704.0
40-41	75900.0
42-43	85823.0
44-45	101448.0
46-47	102234.0
48-49	95410.0
50-51	87136.0
52-53	85569.0
54-55	93963.0
56-57	96377.0
58-59	96722.0
60-61	93777.0
62-63	89441.0
64-65	93524.0
66-67	100114.0
68-69	99988.0
70-71	98494.0
72-73	93585.0
74-75	93923.0
76-77	95690.0
78-79	100755.0
80-81	102479.0
82-83	97391.0
84-85	97263.0
86-87	102941.0
88-89	108235.0
90-91	115580.0
92-93	119574.0
94-95	147183.0
96-97	194811.0
98-99	695695.0
100-101	4728233.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.43564002795486
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.44060736286116	79.95168029316689
2	6.145577628088989	10.746850265068831
3	1.3535893236848262	3.5505584655416813
4	0.48954354475304773	1.7121421262814598
5	0.21324477592025312	0.9322596732602576
6	0.11668997835425621	0.6121717765351554
7	0.06973064714007116	0.4267860633578922
8	0.0406851157752178	0.2845863309934089
9	0.029839222246618363	0.2348110345562519
>10	0.09767845964152264	1.341697438861144
>50	0.0024339238612286454	0.13959214388073266
>100	3.391943905332344E-4	0.044270046381534756
>500	4.0823282247055547E-5	0.022594342114774724
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.1546318808410661E-5	0.0	0.0	0.0
3	0.0	1.1546318808410661E-5	0.0	0.0	0.0
4	0.0	5.773159404205331E-5	0.0	0.0	0.0
5	0.0	5.773159404205331E-5	0.0	0.0	0.0
6	4.6185275233642646E-5	5.773159404205331E-5	0.0	0.0	1.1546318808410661E-5
7	4.6185275233642646E-5	5.773159404205331E-5	0.0	0.0	1.1546318808410661E-5
8	4.6185275233642646E-5	6.927791285046397E-5	0.0	0.0	1.1546318808410661E-5
9	4.6185275233642646E-5	1.3855582570092794E-4	0.0	1.1546318808410661E-5	2.3092637616821323E-5
10-11	4.6185275233642646E-5	1.3855582570092794E-4	0.0	1.1546318808410661E-5	2.3092637616821323E-5
12-13	4.6185275233642646E-5	1.3855582570092794E-4	0.0	1.1546318808410661E-5	2.3092637616821323E-5
14-15	4.6185275233642646E-5	1.8474110093457058E-4	0.0	1.1546318808410661E-5	5.773159404205331E-5
16-17	1.0391686927569595E-4	1.8474110093457058E-4	0.0	1.7319478212615992E-5	9.237055046728529E-5
18-19	1.0391686927569595E-4	2.1938005735980257E-4	0.0	2.3092637616821323E-5	9.237055046728529E-5
20-21	1.1546318808410661E-4	2.4824585438082923E-4	0.0	1.1546318808410661E-4	9.814370987149062E-5
22-23	1.2700950689251728E-4	2.886579702102665E-4	0.0	2.078337385513919E-4	1.2123634748831194E-4
24-25	1.2700950689251728E-4	3.8102852067755185E-4	0.0	3.5216272365652516E-4	1.3855582570092794E-4
26-27	1.2700950689251728E-4	4.041211582943732E-4	0.0	6.177280562499704E-4	1.3855582570092794E-4
28-29	1.2700950689251728E-4	4.041211582943732E-4	0.0	0.0020379252696844817	1.3855582570092794E-4
30-31	1.2700950689251728E-4	4.7339907114483714E-4	0.0	0.005594191462674966	1.3855582570092794E-4
32-33	1.3855582570092794E-4	4.7339907114483714E-4	0.0	0.012412292719041462	1.3855582570092794E-4
34-35	1.6742162272195458E-4	5.542233028037117E-4	0.0	0.023006040225758244	1.3855582570092794E-4
36-37	1.7319478212615992E-4	6.350475344625864E-4	0.0	0.03795274992324585	1.3855582570092794E-4
38-39	1.7319478212615992E-4	6.696864908878184E-4	0.0	0.05678479589976364	1.3855582570092794E-4
40-41	1.7319478212615992E-4	7.909228383761303E-4	0.0	0.07545519341296367	1.3855582570092794E-4
42-43	1.8474110093457058E-4	8.082423165887464E-4	0.0	0.09155076183188815	1.3855582570092794E-4
44-45	1.8474110093457058E-4	9.237055046728529E-4	0.0	0.11104094798048533	1.3855582570092794E-4
46-47	1.905142603387759E-4	0.001027622373948549	0.0	0.12706723848655932	1.3855582570092794E-4
48-49	1.9628741974298124E-4	0.001027622373948549	0.0	0.14009725926185077	1.501021445093386E-4
50-51	2.1938005735980257E-4	0.0011084466056074235	0.0	0.15209388450378947	1.501021445093386E-4
52-53	2.4824585438082923E-4	0.0011084466056074235	0.0	0.16248557143135903	1.6742162272195458E-4
54-55	2.5979217318923987E-4	0.001119992924415834	0.0	0.17275602201144033	1.8474110093457058E-4
56-57	2.6556533259344524E-4	0.0011546318808410662	0.0	0.18185452123246792	1.8474110093457058E-4
58-59	2.7133849199765056E-4	0.0011661781996494768	0.0	0.1904623019041381	1.8474110093457058E-4
60-61	3.0020428901867725E-4	0.0011777245184578875	0.0	0.19927791631435962	1.8474110093457058E-4
62-63	3.348432454439092E-4	0.0012008171560747088	0.0	0.20775868747913723	1.8474110093457058E-4
64-65	3.348432454439092E-4	0.0013393729817756368	0.0	0.215933481195492	1.8474110093457058E-4
66-67	3.348432454439092E-4	0.001345146141179842	0.0	0.22413714070886775	1.8474110093457058E-4
68-69	3.406164048481145E-4	0.0013855582570092796	0.0	0.232381212338073	1.8474110093457058E-4
70-71	3.7525536127334654E-4	0.0014086508946261006	0.0	0.24039435759111	1.9628741974298124E-4
72-73	4.041211582943732E-4	0.0014259703728387169	0.0	0.2472470978039017	1.9628741974298124E-4
74-75	4.041211582943732E-4	0.0014779288074765647	0.0	0.2527200529190884	1.9628741974298124E-4
76-77	4.098943176985785E-4	0.0015241140827102074	0.0	0.2576965163255134	1.9628741974298124E-4
78-79	4.156674771027838E-4	0.0015472067203270287	0.0	0.2621360759073473	1.9628741974298124E-4
80-81	4.156674771027838E-4	0.001552979879731234	0.0	0.26464740024817657	1.9628741974298124E-4
82-83	4.272137959111945E-4	0.0015587530391354394	0.0	0.2660329585051858	1.9628741974298124E-4
84-85	4.329869553153998E-4	0.0015818456767522607	0.0	0.26713563195138906	1.9628741974298124E-4
86-87	4.3876011471960513E-4	0.0016280309519859032	0.0	0.2673607851681531	1.9628741974298124E-4
88-89	4.3876011471960513E-4	0.0016799893866237515	0.0	0.26741274360279094	1.9628741974298124E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9115	0.0	43.979847	1
GTATCAA	14660	0.0	32.36515	1
TCAACGC	16830	0.0	27.288038	4
ATCAACG	17190	0.0	26.764551	3
CAACGCA	17440	0.0	26.404308	5
AACGCAG	18035	0.0	25.66998	6
TATCAAC	18310	0.0	25.396862	2
ACGCAGA	21440	0.0	21.516487	7
CGCAGAG	21875	0.0	20.975842	8
GTACATG	21080	0.0	20.421228	1
TACATGG	21040	0.0	20.06922	2
ACATGGG	21355	0.0	19.118572	3
TATACCG	575	0.0	17.87621	5
GCAGAGT	26405	0.0	17.205978	9
GAGTACT	14815	0.0	16.748615	12-13
CATGGGG	16530	0.0	16.640123	4
CAGAGTA	25555	0.0	16.523338	10-11
AGAGTAC	23030	0.0	16.388983	10-11
GTACTTT	16995	0.0	14.491813	14-15
TATACTG	4630	0.0	14.297107	5
>>END_MODULE