FastQCFastQC Report
Fri 27 May 2016
SRR2139730_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139730_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5003859
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50450.043.4534651
GTATCAA82550.030.31481
TCAACGC96300.025.0113434
ATCAACG98000.024.6607883
CAACGCA98700.024.2790815
AACGCAG102650.023.4243566
TATCAAC106000.023.1076972
GTACATG129200.021.896871
TACATGG126950.021.7704432
ACATGGG131000.020.3494743
ACGCAGA119800.020.0710357
CGCAGAG122950.019.6564228
CATGGGG98700.017.5371914
GAGTACT80150.017.03742212-13
GTGGTAT27500.016.7747881
AGAGTAC129900.016.15344810-11
GCAGAGT151950.015.7974849
CAGAGTA145600.015.64530910-11
TATACTG25400.014.9472245
GTACTTT94500.014.40770614-15
Produced by FastQC (version 0.11.5)