FastQCFastQC Report
Fri 27 May 2016
SRR2139729_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139729_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14008484
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA210100.043.830291
GTATCAA360450.033.2523041
TCAACGC456950.025.7475684
ATCAACG460850.025.5645473
CAACGCA466700.025.2182525
TATCAAC474800.025.007562
AACGCAG471800.024.9626376
CTTATAC188750.024.4152551
GTACATG541600.023.15881
TACATGG543550.022.596512
TATACAC233250.022.2140253
ACATGGG543650.022.0395223
ACGCAGA554200.021.2294317
CGCAGAG558800.021.0833578
TTATACA248000.019.454732
CATGGGG352250.019.2258054
GCAGAGT647600.018.1304939
ACATCTC263500.017.6867458
GAGTACT339550.017.50790412-13
TACACAT294850.017.2196485
Produced by FastQC (version 0.11.5)