FastQCFastQC Report
Fri 27 May 2016
SRR2139728_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139728_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10726092
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108900.046.3765951
GTATCAA193850.034.277161
TCAACGC231750.027.889294
ATCAACG235300.027.5036643
CAACGCA238400.027.1977355
AACGCAG246050.026.4023516
TATCAAC249750.026.1762662
GTACATG310100.022.9284821
TACATGG311500.022.521272
ACGCAGA292900.022.1370437
ACATGGG317350.021.5479453
CGCAGAG301750.021.4331828
CATGGGG229350.018.607814
GCAGAGT365300.017.6932479
GAGTACT193250.017.17039512-13
AGAGTAC319150.016.76673710-11
CAGAGTA348300.016.39836910-11
ATGGGGG132350.015.3135335
GTACTTT218600.015.07593314-15
AGTACTT217700.014.74999712-13
Produced by FastQC (version 0.11.5)