FastQCFastQC Report
Fri 27 May 2016
SRR2139727_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139727_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26182064
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA375050.044.9132231
GTATCAA655500.035.070941
TCAACGC815700.027.6974144
ATCAACG820650.027.5457953
CAACGCA829300.027.248225
AACGCAG844450.026.8432856
TATCAAC850100.026.7975252
ACGCAGA973250.023.2480137
CGCAGAG985000.022.9749188
GTACATG942500.021.1054021
TACATGG940900.020.817882
ACATGGG942450.020.2263073
GCAGAGT1161250.019.4483919
AGAGTAC1039450.018.20433410-11
GAGTACT597950.017.9821812-13
CATGGGG611750.017.7979344
CAGAGTA1130550.017.16881210-11
AGTACTT658450.016.47553812-13
GTACTTT679200.015.85628814-15
ATGGGGG335950.014.1449245
Produced by FastQC (version 0.11.5)