##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139727_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26182064
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.43347590930952	34.0	31.0	34.0	31.0	34.0
2	32.53905490415118	34.0	31.0	34.0	31.0	34.0
3	32.614771738393124	34.0	31.0	34.0	31.0	34.0
4	36.15290605049319	37.0	35.0	37.0	35.0	37.0
5	36.07709812335651	37.0	35.0	37.0	35.0	37.0
6	36.10680987564617	37.0	35.0	37.0	35.0	37.0
7	36.02317158800009	37.0	35.0	37.0	35.0	37.0
8	36.00123672449964	37.0	35.0	37.0	35.0	37.0
9	37.69697453951682	39.0	37.0	39.0	35.0	39.0
10-11	37.59633757292779	39.0	37.0	39.0	35.0	39.0
12-13	37.56247589953183	39.0	37.0	39.0	35.0	39.0
14-15	38.79727618112919	40.0	38.0	41.0	35.0	41.0
16-17	38.857809567649056	40.0	38.0	41.0	35.0	41.0
18-19	38.859215530143075	40.0	38.0	41.0	35.0	41.0
20-21	38.836309641062364	40.0	38.0	41.0	35.0	41.0
22-23	38.74246975214115	40.0	38.0	41.0	34.5	41.0
24-25	38.66726956667474	40.0	38.0	41.0	34.0	41.0
26-27	38.5360665556605	40.0	38.0	41.0	34.0	41.0
28-29	38.40543861426015	40.0	38.0	41.0	34.0	41.0
30-31	38.25465063960743	40.0	38.0	41.0	34.0	41.0
32-33	38.448682513253246	40.0	38.0	41.0	34.0	41.0
34-35	38.43081782010341	40.0	38.0	41.0	34.0	41.0
36-37	38.338711571444435	40.0	38.0	41.0	34.0	41.0
38-39	38.23147929883331	40.0	38.0	41.0	34.0	41.0
40-41	38.19499409361063	40.0	38.0	41.0	33.5	41.0
42-43	38.0976268719724	40.0	37.5	41.0	33.0	41.0
44-45	38.12853623463165	40.0	37.5	41.0	33.5	41.0
46-47	38.24234385395755	40.0	38.0	41.0	33.0	41.0
48-49	38.18077312104219	40.0	38.0	41.0	33.0	41.0
50-51	38.08611748855242	40.0	37.0	41.0	33.0	41.0
52-53	37.95042802117419	40.0	37.0	41.0	33.0	41.0
54-55	37.84643345213124	40.0	37.0	41.0	33.0	41.0
56-57	37.7043219858742	40.0	36.5	41.0	33.0	41.0
58-59	37.55733750500253	39.0	36.0	41.0	32.5	41.0
60-61	37.32865122111774	39.0	36.0	41.0	32.0	41.0
62-63	37.09207042788293	39.0	35.0	40.0	31.5	41.0
64-65	36.86029455411834	38.0	35.0	40.0	31.0	41.0
66-67	36.57912546931422	38.0	35.0	40.0	31.0	41.0
68-69	36.26520413891625	37.0	35.0	40.0	31.0	41.0
70-71	35.96240684154107	37.0	34.5	39.0	31.0	41.0
72-73	35.63779500282906	36.0	34.0	39.0	30.5	40.5
74-75	35.237793766874745	36.0	34.0	39.0	30.0	40.0
76-77	34.220171410045694	35.0	33.0	37.0	29.0	39.0
78-79	34.50556384014715	35.0	34.0	37.0	29.5	39.0
80-81	34.371768676890525	35.0	34.0	37.0	30.0	39.0
82-83	34.10842177153174	35.0	34.0	36.0	29.5	38.5
84-85	33.850148435728755	35.0	33.0	36.0	29.0	37.0
86-87	33.62694721316392	35.0	33.0	35.5	29.0	37.0
88-89	33.38279447375116	35.0	33.0	35.0	29.0	36.5
90-91	33.24663810980649	35.0	33.0	35.0	29.0	36.0
92-93	33.15001779021357	35.0	33.0	35.0	29.0	36.0
94-95	33.08741476215126	34.0	33.0	35.0	29.0	36.0
96-97	33.0296144340726	34.0	33.0	35.0	29.0	35.0
98-99	33.10420719392924	34.0	33.0	35.0	29.0	35.0
100-101	32.299515474583195	33.5	31.5	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	2.0
18	4.0
19	22.0
20	119.0
21	558.0
22	2808.0
23	9826.0
24	25880.0
25	56417.0
26	104146.0
27	168962.0
28	249968.0
29	343564.0
30	453874.0
31	586718.0
32	758180.0
33	999813.0
34	1388941.0
35	1992141.0
36	3205297.0
37	5577684.0
38	6744748.0
39	3370508.0
40	141882.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.71938194024734	21.573810987552395	14.434778709577673	24.272028362622596
2	14.074282727500332	22.90095922789688	40.567974289201416	22.456783755401375
3	16.707165903259778	28.132186361036553	31.800064739195573	23.360582996508093
4	11.7839334591803	19.382646837926913	39.9086145385635	28.924805164329292
5	12.224204325526054	38.89795701362581	36.316170489843735	12.561668171004396
6	28.36512430799955	39.752931625253076	19.24952517112478	12.63241889562259
7	25.061901154928044	33.567044217751516	24.363720904509286	17.007333722811158
8	24.351838724403088	36.54090449095228	21.922274729753926	17.184982054890707
9	25.80264489461182	15.9141616948152	21.370973655858453	36.91221975471453
10-11	23.51676132179648	27.06845228092025	29.91683734330494	19.497949053978328
12-13	24.612668046338896	24.86961875885721	29.72380061403868	20.793912580765213
14-15	21.78670217597104	26.073322807550113	27.32207682069912	24.81789819577972
16-17	21.19808974556702	29.2123763972448	27.884112030595603	21.705421826592573
18-19	21.27011290276532	28.402799361432557	29.5434361165673	20.783651619234824
20-21	22.281375032834084	27.56614724039187	29.202383740221343	20.9500939865527
22-23	21.87344489782275	27.595156996497394	29.073143105453035	21.45825500022682
24-25	21.54025111856693	27.776159292199743	29.197847723272897	21.485741865960428
26-27	21.521743127517905	27.903670439662175	29.126978311210376	21.447608121609544
28-29	21.47286496334837	27.942970142993413	29.10751950946697	21.476645384191244
30-31	21.71545422838237	27.813414016381792	29.050475964956	21.420655790279834
32-33	21.474803848715098	28.00443924486759	29.01956372256467	21.501193183852646
34-35	21.590194803016544	28.08313607444337	29.04210397099857	21.284565151541514
36-37	21.60573262203971	28.006389159041817	28.919177916889826	21.46870030202864
38-39	21.49435511554298	28.086104027616425	28.967371863337803	21.452168993502788
40-41	21.678966286829322	27.979098244661145	28.865855897734267	21.47607957077527
42-43	21.65346038556448	28.06158621346373	28.666370114039335	21.61858328693246
44-45	21.68505953924897	28.20308567526824	28.465056605142454	21.646798180340333
46-47	21.811246089645937	28.147506677202376	28.1763126246832	21.864934608468488
48-49	21.73948837462921	28.253776782147792	28.13755245442301	21.86918238879999
50-51	21.749526426350496	28.38274977736233	27.9916472919247	21.87607650436247
52-53	21.876721767346645	28.27783236489814	27.935847382563367	21.909598485191857
54-55	21.8479227579107	28.221074536719755	27.962265364962434	21.968737340407113
56-57	21.916601088438203	28.288792532127133	27.880539457007565	21.914066922427104
58-59	21.836933101965315	28.333924362976866	27.930578785728606	21.898563749329213
60-61	21.918087220556046	28.32288666790714	27.886349479901074	21.872676631635734
62-63	21.848368484678865	28.364803605585404	27.858416094273338	21.928411815462397
64-65	21.814446696831084	28.30300624110237	27.86373531670567	22.01881174536088
66-67	21.877004350799723	28.344167694599797	27.72969341097145	22.04913454362903
68-69	21.910379033947187	28.30409796282029	27.75429685011474	22.03122615311778
70-71	21.90426524182901	28.06433728656114	27.83088735902881	22.200510112581036
72-73	21.90317413979656	28.073484539505362	27.82601927666478	22.197322044033303
74-75	21.85964177078807	28.10203520384778	27.78670539843922	22.251617626924933
76-77	21.950204841657545	28.104486629535074	27.75212049705561	22.19318803175177
78-79	21.93487273785295	28.07455707369007	27.70379447310439	22.28677571535259
80-81	21.947831220669297	28.056399384816693	27.702411058611958	22.29335833590205
82-83	21.97868893851016	27.99854412242844	27.79102878286549	22.231738156195906
84-85	22.044254390473114	28.009247362616208	27.7552089961463	22.191289250764378
86-87	21.92591589533278	28.001108234949633	27.8323592139849	22.24061665573268
88-89	21.975071047805443	27.913394927938118	27.83498593207859	22.276548092177844
90-91	21.983685592412254	27.882695456378727	27.94143319987286	22.19218575133616
92-93	21.9468871316375	27.814062318568418	27.97487524854299	22.264175301251097
94-95	21.8162565859038	27.86317602828096	28.021886862324475	22.29868052349076
96-97	21.775426780292	27.90245440794486	28.117999909122098	22.20411890264104
98-99	21.959046121965432	28.51653844650688	28.154107037853265	21.370308393674424
100-101	21.449051171595347	31.659408783317044	25.726513742848145	21.16502630223946
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3479.0
1	3745.5
2	7220.0
3	11043.0
4	15261.0
5	24330.0
6	31090.5
7	34890.0
8	36407.5
9	34678.0
10	31821.0
11	29889.0
12	35073.0
13	50831.0
14	84060.5
15	126301.5
16	158083.0
17	173545.5
18	172941.0
19	161066.5
20	144866.5
21	131166.0
22	127803.0
23	140347.5
24	168660.5
25	211530.5
26	266893.0
27	326031.5
28	387403.0
29	452039.5
30	512656.5
31	574314.5
32	638702.0
33	700171.5
34	761475.5
35	819653.0
36	879675.5
37	931985.5
38	968149.0
39	1000325.5
40	1031808.0
41	1058416.0
42	1076922.0
43	1094032.0
44	1110067.0
45	1117867.5
46	1120552.5
47	1121118.5
48	1109653.5
49	1086893.5
50	1056655.5
51	1012163.0
52	952920.0
53	889498.0
54	823727.0
55	753131.5
56	684267.0
57	614012.5
58	540015.5
59	465143.5
60	389952.5
61	316258.5
62	249514.5
63	193177.0
64	145095.0
65	107768.0
66	80677.0
67	61167.0
68	46345.5
69	33483.5
70	23969.0
71	17482.5
72	12669.0
73	9044.5
74	6383.0
75	4490.5
76	3121.5
77	2155.5
78	1480.5
79	1031.5
80	752.5
81	556.5
82	436.0
83	313.5
84	222.0
85	175.0
86	142.5
87	117.0
88	85.5
89	57.5
90	42.5
91	33.5
92	28.0
93	22.5
94	13.5
95	7.5
96	6.0
97	5.5
98	3.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	2.6353919232647205E-4
3	3.399273640153045E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	2.291645150664974E-5
16-17	7.638817168883247E-6
18-19	8.21172845654949E-5
20-21	0.0
22-23	1.3179522826398715E-4
24-25	0.0020729517378815384
26-27	0.0030789887912767255
28-29	0.0030058891908779214
30-31	0.004639580871408262
32-33	0.002294254782849265
34-35	0.006130637173382454
36-37	0.022449460629967773
38-39	0.009795697269244496
40-41	0.00386064914119545
42-43	0.00721871663130369
44-45	0.004142969732466275
46-47	0.0033566889571390824
48-49	0.0021839481373336442
50-51	9.346224726484291E-5
52-53	5.243492919669563E-5
54-55	0.002662544421421266
56-57	0.0025255480786834186
58-59	0.004210706248054737
60-61	0.001886697744637581
62-63	0.0011432735357784025
64-65	2.862216071240379E-4
66-67	5.2048174885488355E-5
68-69	1.191626568432639E-4
70-71	2.2284123597220222E-4
72-73	0.0012274235483841311
74-75	0.0033905025072337464
76-77	0.003908275357166579
78-79	0.0013826941800985065
80-81	0.006374614358128858
82-83	0.004310639512905276
84-85	0.00714767202565331
86-87	0.003646397600970366
88-89	0.056673664796664226
90-91	5.341979933279739E-6
92-93	1.7688166442489568E-4
94-95	0.0015026999713125965
96-97	0.025659433613202148
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	689.0
20-21	3061.0
22-23	5841.0
24-25	8501.0
26-27	11679.0
28-29	25141.0
30-31	64662.0
32-33	84433.0
34-35	117905.0
36-37	182859.0
38-39	213198.0
40-41	220162.0
42-43	251518.0
44-45	294857.0
46-47	292188.0
48-49	268721.0
50-51	243351.0
52-53	211449.0
54-55	237869.0
56-57	248872.0
58-59	256887.0
60-61	266694.0
62-63	248120.0
64-65	258406.0
66-67	277584.0
68-69	277100.0
70-71	279519.0
72-73	265962.0
74-75	261599.0
76-77	265735.0
78-79	283318.0
80-81	290802.0
82-83	279694.0
84-85	278086.0
86-87	291783.0
88-89	311326.0
90-91	341815.0
92-93	358365.0
94-95	444584.0
96-97	585541.0
98-99	2155044.0
100-101	1.4917144E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.97490848497512
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.92392130406309	76.41276983422864
2	6.988479639636946	11.876908356545231
3	1.6926388292650023	4.31495488844727
4	0.6343912473984566	2.1562935276541224
5	0.28196310826239984	1.197989466036828
6	0.15307183157078635	0.7804358927613059
7	0.09848197474374529	0.5857947753888572
8	0.058844135645342535	0.40002200330723553
9	0.039630487469571894	0.30308373413476425
>10	0.12407095106207718	1.6190766665049456
>50	0.0035015559085773964	0.1977775475969618
>100	9.725554209332627E-4	0.127093867255676
>500	1.636229169361096E-5	0.008086724597665839
>1k	1.601726137065957E-5	0.019712715540532624
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.8194085844416235E-6	7.638817168883247E-6
2	0.0	2.2916451506649745E-5	0.0	3.8194085844416235E-6	7.638817168883247E-6
3	0.0	2.2916451506649745E-5	0.0	1.1458225753324872E-5	7.638817168883247E-6
4	0.0	5.7291128766624355E-5	0.0	1.5277634337766494E-5	7.638817168883247E-6
5	0.0	7.256876310439085E-5	0.0	1.5277634337766494E-5	7.638817168883247E-6
6	0.0	7.256876310439085E-5	0.0	1.909704292220812E-5	7.638817168883247E-6
7	0.0	8.02075802732741E-5	0.0	1.909704292220812E-5	7.638817168883247E-6
8	7.638817168883247E-6	8.402698885771572E-5	0.0	2.2916451506649745E-5	1.5277634337766494E-5
9	7.638817168883247E-6	1.3367930045545684E-4	0.0	4.201349442885786E-5	1.909704292220812E-5
10-11	1.909704292220812E-5	1.3367930045545684E-4	0.0	6.111053735106598E-5	1.909704292220812E-5
12-13	2.2916451506649745E-5	1.4513752620878172E-4	0.0	6.874935451994923E-5	2.2916451506649745E-5
14-15	2.2916451506649745E-5	1.7951220346875633E-4	0.0	9.166580602659898E-5	3.24649729677538E-5
16-17	2.2916451506649745E-5	1.8524131634541877E-4	0.0	1.1840166611769032E-4	3.819408584441624E-5
18-19	2.2916451506649745E-5	2.081577678520685E-4	0.0	1.604151605465482E-4	4.77426073055203E-5
20-21	2.2916451506649745E-5	2.406227408198223E-4	0.0	3.4756618118418777E-4	5.347172018218273E-5
22-23	2.2916451506649745E-5	2.711780094953553E-4	0.0	8.307213671160532E-4	5.920083305884516E-5
24-25	2.2916451506649745E-5	3.1701091250865474E-4	0.0	0.0024425117897504183	6.683965022772842E-5
26-27	2.2916451506649745E-5	3.3228854684642125E-4	0.0	0.005664182930726928	6.874935451994923E-5
28-29	2.2916451506649745E-5	3.399273640153045E-4	0.0	0.025660696574571047	6.874935451994923E-5
30-31	2.2916451506649745E-5	3.8194085844416237E-4	0.0	0.03850536764404823	7.065905881217005E-5
32-33	2.2916451506649745E-5	3.85760267028604E-4	0.0	0.04559609968106411	8.020758027327409E-5
34-35	2.864556438331218E-5	4.6405814300965733E-4	0.0	0.05506441356189489	1.0312403177992385E-4
36-37	3.437467725997462E-5	5.557239490362563E-4	0.0	0.06762262898753896	1.0694344036436547E-4
38-39	3.628438155219543E-5	5.939180348806725E-4	0.0	0.08841930872982359	1.107628489488071E-4
40-41	3.819408584441624E-5	6.359315293095304E-4	0.0	0.12310335808513798	1.1267255324102791E-4
42-43	3.819408584441624E-5	6.531188679395176E-4	0.0	0.14128565265137233	1.1649196182546952E-4
44-45	4.0103790136637046E-5	7.180488138750253E-4	0.0	0.16324152289903499	1.1840166611769034E-4
46-47	4.201349442885786E-5	7.753399426416496E-4	0.0	0.1889155874036516	1.1840166611769034E-4
48-49	4.583290301329949E-5	7.887078726871953E-4	0.0	0.21115027447797852	1.2222107470213195E-4
50-51	4.583290301329949E-5	9.147483559737689E-4	0.0	0.23628580237218882	1.2604048328657358E-4
52-53	4.965231159774111E-5	9.242968774348729E-4	0.0	0.2618166390548889	1.413181176243401E-4
54-55	5.156201588996192E-5	9.376648074804186E-4	0.0	0.3366694084927758	1.6041516054654818E-4
56-57	5.347172018218273E-5	9.586715546948476E-4	0.0	0.38354882945821234	1.7187338629987307E-4
58-59	5.347172018218273E-5	9.758588933248349E-4	0.0	0.4327027082356838	1.7951220346875633E-4
60-61	5.538142447440355E-5	0.001004504457708147	0.0	0.46262586479049167	1.8142190776097714E-4
62-63	5.7291128766624355E-5	0.0010197820920459135	0.0	0.4773344072491764	1.8715102063763956E-4
64-65	5.7291128766624355E-5	0.0010904411508580836	0.0	0.49145285108156483	1.8715102063763956E-4
66-67	5.7291128766624355E-5	0.001109538193780292	0.0	0.4992559028195791	1.9669954209874363E-4
68-69	6.111053735106598E-5	0.0011420031667480454	0.0	0.506304621362166	2.0433835926762689E-4
70-71	6.49299459355076E-5	0.001155371096793591	0.0	0.5132979584802787	2.1770628931317256E-4
72-73	6.49299459355076E-5	0.0011611002096702536	0.0	0.5189239473251612	2.444421494042639E-4
74-75	6.49299459355076E-5	0.0011725584354235785	0.0	0.5240018510381763	2.444421494042639E-4
76-77	6.49299459355076E-5	0.0012145719298524364	0.0	0.5286443421725652	2.444421494042639E-4
78-79	6.683965022772842E-5	0.0012374883813590861	0.0	0.5328915245184642	2.444421494042639E-4
80-81	6.874935451994923E-5	0.0012432174942357487	0.0	0.535250009319357	2.5208096657314716E-4
82-83	6.874935451994923E-5	0.0012546757199890734	0.0	0.5367013845814448	2.597197837420304E-4
84-85	6.874935451994923E-5	0.0012756824672035024	0.0	0.5380572746289216	2.597197837420304E-4
86-87	6.874935451994923E-5	0.0012890503972490482	0.0	0.5382539741710203	2.673586009109137E-4
88-89	6.874935451994923E-5	0.0013329735959701266	0.0	0.5382883488482803	2.807265309564594E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	37505	0.0	44.913223	1
GTATCAA	65550	0.0	35.07094	1
TCAACGC	81570	0.0	27.697414	4
ATCAACG	82065	0.0	27.545795	3
CAACGCA	82930	0.0	27.24822	5
AACGCAG	84445	0.0	26.843285	6
TATCAAC	85010	0.0	26.797525	2
ACGCAGA	97325	0.0	23.248013	7
CGCAGAG	98500	0.0	22.974918	8
GTACATG	94250	0.0	21.105402	1
TACATGG	94090	0.0	20.81788	2
ACATGGG	94245	0.0	20.226307	3
GCAGAGT	116125	0.0	19.448391	9
AGAGTAC	103945	0.0	18.204334	10-11
GAGTACT	59795	0.0	17.98218	12-13
CATGGGG	61175	0.0	17.797934	4
CAGAGTA	113055	0.0	17.168812	10-11
AGTACTT	65845	0.0	16.475538	12-13
GTACTTT	67920	0.0	15.856288	14-15
ATGGGGG	33595	0.0	14.144924	5
>>END_MODULE