FastQCFastQC Report
Fri 27 May 2016
SRR2139726_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139726_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9106746
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91550.043.6233671
GTATCAA152050.031.2920061
TCAACGC176400.026.1339784
ATCAACG179750.025.6469213
CAACGCA183500.025.100415
AACGCAG189550.024.3426156
TATCAAC191950.024.2737142
GTACATG241400.022.535231
TACATGG244150.022.0794562
ACATGGG246300.021.1527163
ACGCAGA223700.020.6081097
CGCAGAG229250.020.1271238
CATGGGG171100.017.9863534
GAGTACT149700.016.56419612-13
GCAGAGT280200.016.5260649
AGAGTAC246050.016.08113110-11
CAGAGTA269350.015.82649510-11
TATACCG6600.014.9409625
ATGGGGG97150.014.5910845
GAGTACA176500.014.5261721
Produced by FastQC (version 0.11.5)