FastQCFastQC Report
Fri 27 May 2016
SRR2139725_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139725_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8358586
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79050.041.8272171
GTATCAA129050.032.545241
TCAACGC150100.026.9112344
ATCAACG153800.026.237053
CAACGCA154200.026.1956945
AACGCAG159300.025.4862796
TATCAAC166300.024.5620542
GTACATG207450.021.9129521
TACATGG209150.021.4001562
ACGCAGA193000.021.0360837
ACATGGG209650.020.917033
CGCAGAG197700.020.5359848
CATGGGG151100.018.9666254
AGAGTAC211350.016.4626510-11
GCAGAGT245500.016.3530379
GAGTACT134550.015.91351812-13
CAGAGTA234400.015.4673910-11
ATGGGGG90800.015.1009285
GTGGTAT45200.014.5756131
GAGTACA149600.014.3400751
Produced by FastQC (version 0.11.5)