##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139725_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8358586
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.43593222585734	34.0	31.0	34.0	31.0	34.0
2	32.5501468789099	34.0	31.0	34.0	31.0	34.0
3	32.63084772950832	34.0	31.0	34.0	31.0	34.0
4	36.16062920211624	37.0	35.0	37.0	35.0	37.0
5	36.07628000716868	37.0	35.0	37.0	35.0	37.0
6	36.101430672604195	37.0	35.0	37.0	35.0	37.0
7	36.01718065711114	37.0	35.0	37.0	35.0	37.0
8	35.99476873241479	37.0	35.0	37.0	35.0	37.0
9	37.693579272857875	39.0	37.0	39.0	35.0	39.0
10-11	37.594335393570155	39.0	37.0	39.0	35.0	39.0
12-13	37.55866434825221	39.0	37.0	39.0	35.0	39.0
14-15	38.820475795786514	40.0	38.0	41.0	35.0	41.0
16-17	38.869875658394854	40.0	38.0	41.0	35.0	41.0
18-19	38.84520055186368	40.0	38.0	41.0	35.0	41.0
20-21	38.81125216347098	40.0	38.0	41.0	35.0	41.0
22-23	38.71636500193054	40.0	38.0	41.0	34.0	41.0
24-25	38.642798083642994	40.0	38.0	41.0	34.0	41.0
26-27	38.51303460223035	40.0	38.0	41.0	34.0	41.0
28-29	38.38247458220872	40.0	38.0	41.0	34.0	41.0
30-31	38.24009509523252	40.0	38.0	41.0	34.0	41.0
32-33	38.468228152041206	40.0	38.0	41.0	34.0	41.0
34-35	38.45702307425586	40.0	38.0	41.0	34.0	41.0
36-37	38.36372886205065	40.0	38.0	41.0	34.0	41.0
38-39	38.25906970818167	40.0	38.0	41.0	34.0	41.0
40-41	38.220568392629666	40.0	38.0	41.0	34.0	41.0
42-43	38.122207612646065	40.0	38.0	41.0	33.0	41.0
44-45	38.1496507948558	40.0	37.5	41.0	33.5	41.0
46-47	38.262814274990845	40.0	38.0	41.0	33.5	41.0
48-49	38.19435545105105	40.0	38.0	41.0	33.0	41.0
50-51	38.095267783524406	40.0	37.0	41.0	33.0	41.0
52-53	37.95245636420642	40.0	37.0	41.0	33.0	41.0
54-55	37.84356955778273	40.0	37.0	41.0	33.0	41.0
56-57	37.70047148402613	39.5	36.0	41.0	33.0	41.0
58-59	37.54592421019342	39.0	36.0	41.0	32.5	41.0
60-61	37.30821644737853	39.0	35.5	41.0	32.0	41.0
62-63	37.06067655413378	39.0	35.0	40.0	32.0	41.0
64-65	36.814601091789825	38.0	35.0	40.0	31.0	41.0
66-67	36.52470281019019	38.0	35.0	40.0	31.0	41.0
68-69	36.19996880175014	37.0	35.0	40.0	31.0	41.0
70-71	35.89231820735206	37.0	34.0	39.0	31.0	41.0
72-73	35.56221830200411	36.0	34.0	39.0	30.0	40.5
74-75	35.16188441430965	36.0	34.0	38.5	30.0	40.0
76-77	34.15542648937097	35.0	32.5	37.0	29.0	39.0
78-79	34.4412032060288	35.0	34.0	37.0	29.5	39.0
80-81	34.313570768876446	35.0	34.0	36.5	30.0	39.0
82-83	34.060646026608424	35.0	34.0	36.0	29.5	37.5
84-85	33.81283183510169	35.0	33.0	36.0	29.0	37.0
86-87	33.596585815616606	35.0	33.0	35.0	29.0	37.0
88-89	33.36203598000816	35.0	33.0	35.0	29.0	36.0
90-91	33.23134610484699	35.0	33.0	35.0	29.0	36.0
92-93	33.13698565009747	35.0	33.0	35.0	29.0	36.0
94-95	33.07846457815954	34.0	33.0	35.0	29.0	35.5
96-97	33.02283651864711	34.0	33.0	35.0	29.0	35.0
98-99	33.09900080383184	34.0	33.0	35.0	29.0	35.0
100-101	32.2463619765904	33.5	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	29.0
21	192.0
22	927.0
23	2998.0
24	8041.0
25	17510.0
26	32423.0
27	52611.0
28	78528.0
29	108323.0
30	143963.0
31	186612.0
32	241442.0
33	320137.0
34	442070.0
35	636543.0
36	1018263.0
37	1751553.0
38	2125245.0
39	1140052.0
40	51119.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.090082222040905	20.95856882970397	13.696598922353614	25.25475002590151
2	14.731301494310808	22.3766053734796	39.583976635235054	23.30811649697454
3	17.576692056213524	26.575038421699055	30.96341498138794	24.884854540699482
4	12.425175741447179	18.395120897242666	38.5853061749918	30.594397186318357
5	12.80122020638419	38.1072588114784	36.2000941307537	12.891426851383713
6	30.281293989198648	38.36284031772838	18.47826893208971	12.877596760983256
7	26.290427591461047	32.52771461584531	23.749686848947896	17.43217094374575
8	24.624320429316633	36.7156358742974	21.421888821865327	17.238154874520642
9	25.73394590903294	15.969315862754776	21.08167577626168	37.2150624519506
10-11	24.109484546788178	26.694796225103147	29.469745241599476	19.725973986509203
12-13	24.750460185490702	24.278029800734238	29.353397811543726	21.618112202231334
14-15	21.890631009913758	25.769941921987993	26.58150273042626	25.757924337671987
16-17	21.747704800052592	28.751335976643418	27.41900528504494	22.08195393825905
18-19	21.802821329684548	28.177549131721175	28.722647929345385	21.296981609248895
20-21	22.470490403170853	27.48712335965322	28.529571750947312	21.512814486228613
22-23	22.204142329557676	27.40647888346911	28.372054037939414	22.0173247490338
24-25	22.023889491935233	27.511023440493528	28.46815662965028	21.99693043792096
26-27	21.981325732881473	27.68077862726117	28.38086309668127	21.957032543176087
28-29	21.989080051943613	27.719147456678613	28.367933456199623	21.92383903517815
30-31	22.196773715961555	27.61876127336167	28.275450712508853	21.90901429816792
32-33	21.94856088652775	27.75309860716286	28.307181459201736	21.991159047107658
34-35	22.11397722730779	27.75404729137014	28.289473247210356	21.84250223411172
36-37	22.103141858743676	27.664126176214026	28.254120497127555	21.978611467914742
38-39	21.937485964920665	27.81068811183911	28.330284666018095	21.92154125722213
40-41	22.18065523459514	27.65787939573366	28.207773013642303	21.9536923560289
42-43	22.0293078181303	27.764586027926192	28.06176669275921	22.144339461184302
44-45	22.11450865461839	27.825965758074457	27.930398602790106	22.129126984517047
46-47	22.174085832808654	27.79922417868003	27.77802018207114	22.248669806440173
48-49	22.061302195570782	27.902596702746674	27.776738466132283	22.259362635550257
50-51	22.125935352280887	27.97071333465765	27.71242607964432	22.190925233417143
52-53	22.27274279053984	27.87911716456944	27.597551408036786	22.250588636853934
54-55	22.221863831740077	27.796000041671242	27.645910114598582	22.3362260119901
56-57	22.23142526362646	27.905522843479723	27.60266307817914	22.260388814714673
58-59	22.22034369976436	27.944813346995645	27.565477349675522	22.269365603564474
60-61	22.296838707077438	27.91764100958446	27.563142380502548	22.222377902835554
62-63	22.245521649644413	27.964243432567542	27.5636838529911	22.226551064796944
64-65	22.270132005339093	27.898325381818488	27.509484572591557	22.322058040250855
66-67	22.290431909131694	27.947740634701557	27.420296540006234	22.34153091616051
68-69	22.272435003226658	27.91195857888994	27.485479667807223	22.33012675007618
70-71	22.24485405851837	27.730175451891707	27.557780823094074	22.467189666495845
72-73	22.287668757237235	27.784104749686183	27.482586078383875	22.44564041469271
74-75	22.158211346863464	27.927677183267985	27.456657117904705	22.457454351963854
76-77	22.286570747114524	27.8314313379138	27.42487401664929	22.457123898322386
78-79	22.258213139767797	27.83061341102746	27.401197435591527	22.509976013613215
80-81	22.25682182243277	27.850810698239613	27.401751190160777	22.49061628916683
82-83	22.259342335293695	27.834766761611263	27.510727934937616	22.39516296815743
84-85	22.260131547469403	27.824117946166016	27.486067315546897	22.42968319081768
86-87	22.198675866258665	27.840567286824047	27.508869704461254	22.45188714245603
88-89	22.26415354138464	27.823243510497974	27.447961037191575	22.46464191092581
90-91	22.26872835225376	27.847451435914365	27.502021674495747	22.381798537336124
92-93	22.27943863198377	27.825541884986638	27.496845117674113	22.398174365355477
94-95	22.119204773319197	27.864148175144628	27.587153482033788	22.429493569502384
96-97	22.091108760677017	27.900788935779318	27.632279244156415	22.375823059387248
98-99	22.263679951971387	28.437212401341423	27.711460276916906	21.587647369770284
100-101	24.91600967568536	36.277673539281004	28.986829808115193	9.81948697691844
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2110.0
1	1895.0
2	2252.5
3	3014.5
4	3845.0
5	5608.0
6	7096.5
7	8188.5
8	8843.0
9	8733.5
10	8330.0
11	8024.5
12	8985.5
13	11839.5
14	17953.5
15	25786.0
16	31778.5
17	34842.5
18	35851.5
19	35686.5
20	34794.0
21	34182.5
22	35390.5
23	40184.5
24	48436.5
25	60015.0
26	75471.5
27	93184.5
28	112854.5
29	134031.5
30	154542.0
31	174391.0
32	194291.0
33	213959.0
34	232078.0
35	249087.5
36	268273.5
37	285067.5
38	298630.5
39	311538.5
40	323194.0
41	335395.5
42	344866.5
43	351692.5
44	357858.0
45	361245.0
46	363243.0
47	365173.5
48	364173.0
49	359333.5
50	352973.0
51	343486.0
52	328793.0
53	311704.5
54	293105.5
55	272435.0
56	250835.5
57	228189.5
58	203168.5
59	176216.5
60	148614.0
61	121810.5
62	97939.0
63	77068.0
64	58629.5
65	44328.5
66	33777.5
67	25962.5
68	19664.0
69	14380.5
70	10607.5
71	7781.0
72	5514.5
73	3864.0
74	2701.0
75	1852.5
76	1319.0
77	944.5
78	647.5
79	430.0
80	298.0
81	221.0
82	179.5
83	135.0
84	101.5
85	76.0
86	53.5
87	36.0
88	20.5
89	14.5
90	9.5
91	5.0
92	4.0
93	3.5
94	2.5
95	1.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	2.0338368235967185E-4
3	3.349848885924007E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.1963746021157168E-5
16-17	5.981873010578584E-6
18-19	1.1365558720099309E-4
20-21	0.0
22-23	9.572402571937054E-5
24-25	0.002082476231795163
26-27	0.0031248518389214303
28-29	0.0034203788809394862
30-31	0.005146758807645949
32-33	0.002429996989570561
34-35	0.0065876216475889305
36-37	0.021485624313198077
38-39	0.00956713433334591
40-41	0.003521745698450216
42-43	0.007431302378758645
44-45	0.004460912014559207
46-47	0.0034892242612460664
48-49	0.002214900923542653
50-51	1.241136002638002E-4
52-53	3.961993128187052E-5
54-55	0.002778005568298469
56-57	0.0027718783342823073
58-59	0.004690379811893174
60-61	0.00209470813606142
62-63	0.0011307773504313423
64-65	2.844474785508823E-4
66-67	3.6035958120450915E-5
68-69	1.023291054601568E-4
70-71	1.6313285072209644E-4
72-73	0.001015280039804994
74-75	0.0029051874905829224
76-77	0.003240350734326118
78-79	0.0011537821252776631
80-81	0.006330220496568327
82-83	0.0046969332184366285
84-85	0.007335158954621434
86-87	0.0037257677859257364
88-89	0.05407064914341558
90-91	1.7299596928041374E-5
92-93	2.2925308111732307E-4
94-95	0.001575547928303026
96-97	0.024842125286840967
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	204.0
20-21	732.0
22-23	1535.0
24-25	2781.0
26-27	4529.0
28-29	8693.0
30-31	20899.0
32-33	29214.0
34-35	42534.0
36-37	66081.0
38-39	71218.0
40-41	71105.0
42-43	80447.0
44-45	95400.0
46-47	97141.0
48-49	90599.0
50-51	83283.0
52-53	82756.0
54-55	90315.0
56-57	93646.0
58-59	93712.0
60-61	91025.0
62-63	87285.0
64-65	91835.0
66-67	96598.0
68-69	97682.0
70-71	96094.0
72-73	91369.0
74-75	91423.0
76-77	93561.0
78-79	98661.0
80-81	98436.0
82-83	94098.0
84-85	94079.0
86-87	98350.0
88-89	103777.0
90-91	109338.0
92-93	113441.0
94-95	140093.0
96-97	188861.0
98-99	671786.0
100-101	4583970.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.64462551844446
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.06022996166814	81.60644610093952
2	5.791525186101461	10.267751626052068
3	1.2619031794742324	3.355828043550832
4	0.41196903521739364	1.4607536340816285
5	0.1908728462236012	0.8459925987565382
6	0.097324074995717	0.5176353709154602
7	0.05356280830153016	0.3323638558523758
8	0.03251002317957534	0.23054710642795243
9	0.021392701695930483	0.1706713227597194
>10	0.07687914130208208	1.0771348286951978
>50	0.001616914930696676	0.09779563192885622
>100	1.9448437181812878E-4	0.027921139573413543
>500	1.964253784908429E-5	0.009158740466404369
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1963746021157166E-5
2	0.0	2.3927492042314333E-5	0.0	0.0	1.1963746021157166E-5
3	0.0	2.3927492042314333E-5	0.0	1.1963746021157166E-5	1.1963746021157166E-5
4	0.0	9.570996816925733E-5	0.0	1.1963746021157166E-5	2.3927492042314333E-5
5	0.0	1.1963746021157167E-4	0.0	3.58912380634715E-5	2.3927492042314333E-5
6	0.0	1.1963746021157167E-4	0.0	3.58912380634715E-5	2.3927492042314333E-5
7	0.0	1.3160120623272883E-4	0.0	3.58912380634715E-5	2.3927492042314333E-5
8	0.0	1.3160120623272883E-4	0.0	3.58912380634715E-5	2.3927492042314333E-5
9	0.0	2.3927492042314334E-4	0.0	3.58912380634715E-5	3.58912380634715E-5
10-11	0.0	2.3927492042314334E-4	0.0	5.9818730105785835E-5	3.58912380634715E-5
12-13	0.0	2.3927492042314334E-4	0.0	8.374622214810018E-5	3.58912380634715E-5
14-15	0.0	2.7516615848661486E-4	0.0	8.972809515867875E-5	4.7854984084628666E-5
16-17	0.0	2.8114803149719347E-4	0.0	1.1365558720099309E-4	8.374622214810018E-5
18-19	0.0	3.230211425712435E-4	0.0	1.43564952253886E-4	8.374622214810018E-5
20-21	0.0	3.58912380634715E-4	0.0	2.0936555537025042E-4	8.972809515867875E-5
22-23	0.0	4.30694856761658E-4	0.0	2.8712990450777197E-4	1.1963746021157167E-4
24-25	0.0	4.606042218145509E-4	0.0	4.067673647193437E-4	1.3160120623272883E-4
26-27	0.0	4.905135868674439E-4	0.0	6.280966661107513E-4	1.3160120623272883E-4
28-29	0.0	4.905135868674439E-4	0.0	0.001621087585866796	1.3758307924330743E-4
30-31	0.0	5.862235550367011E-4	0.0	0.005090573932002375	1.495468252644646E-4
32-33	0.0	6.280966661107513E-4	0.0	0.01283709948070164	1.6749244429620035E-4
34-35	0.0	7.058610152482728E-4	0.0	0.024758972390784756	1.794561903173575E-4
36-37	0.0	8.135347294386874E-4	0.0	0.040365679075384284	1.9141993633851466E-4
38-39	0.0	8.314803484704231E-4	0.0	0.05985462134384931	1.9141993633851466E-4
40-41	0.0	8.853172055656303E-4	0.0	0.07850610139083333	1.9141993633851466E-4
42-43	0.0	9.152265706185232E-4	0.0	0.09587147874054296	1.9141993633851466E-4
44-45	0.0	0.0010528096498618306	0.0	0.1165867049761766	1.9740180934909327E-4
46-47	0.0	0.0011604833640522453	0.0	0.13107480140779793	2.0936555537025042E-4
48-49	0.0	0.0011604833640522453	0.0	0.1426437438102569	2.2731117440198619E-4
50-51	5.981873010578583E-6	0.0013160120623272884	0.0	0.15358458954660514	2.3927492042314334E-4
52-53	1.1963746021157166E-5	0.0013399395543696028	0.0	0.1627009640147269	2.572205394548791E-4
54-55	1.1963746021157166E-5	0.0013519033003907598	0.0	0.17217026899047277	2.9311177751835057E-4
56-57	1.7945619031735748E-5	0.001363867046411917	0.0	0.18114307850634068	2.990936505289292E-4
58-59	2.3927492042314333E-5	0.001363867046411917	0.0	0.18969117503845745	3.050755235395078E-4
60-61	2.3927492042314333E-5	0.0013758307924330742	0.0	0.19807776099928864	3.1105739655008633E-4
62-63	2.3927492042314333E-5	0.0014057401574859671	0.0	0.20594392400819947	3.1105739655008633E-4
64-65	2.3927492042314333E-5	0.0014954682526446459	0.0	0.21299057041466105	3.1105739655008633E-4
66-67	2.3927492042314333E-5	0.001507431998665803	0.0	0.2208507515505613	3.1105739655008633E-4
68-69	2.3927492042314333E-5	0.0015433232367292745	0.0	0.2286750414483981	3.1105739655008633E-4
70-71	2.3927492042314333E-5	0.0015552869827504317	0.0	0.23663093255246762	3.230211425712435E-4
72-73	2.3927492042314333E-5	0.0015612688557610104	0.0	0.24340839467345315	3.349848885924007E-4
74-75	2.3927492042314333E-5	0.0016031419668350606	0.0	0.24907322841447105	3.349848885924007E-4
76-77	2.3927492042314333E-5	0.0016569788239302677	0.0	0.25446887787001293	3.349848885924007E-4
78-79	2.3927492042314333E-5	0.0016988519350043178	0.0	0.25927232189750754	3.349848885924007E-4
80-81	2.3927492042314333E-5	0.0016988519350043178	0.0	0.2622393309107545	3.409667616029793E-4
82-83	2.9909365052892918E-5	0.0017108156810254748	0.0	0.26396809221081174	3.4694863461355786E-4
84-85	3.58912380634715E-5	0.0017467069190889464	0.0	0.26530204989217077	3.4694863461355786E-4
86-87	3.58912380634715E-5	0.0017825981571524179	0.0	0.26549945170151984	3.4694863461355786E-4
88-89	3.58912380634715E-5	0.0018663443793005181	0.0	0.2655473066856045	3.4694863461355786E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7905	0.0	41.827217	1
GTATCAA	12905	0.0	32.54524	1
TCAACGC	15010	0.0	26.911234	4
ATCAACG	15380	0.0	26.23705	3
CAACGCA	15420	0.0	26.195694	5
AACGCAG	15930	0.0	25.486279	6
TATCAAC	16630	0.0	24.562054	2
GTACATG	20745	0.0	21.912952	1
TACATGG	20915	0.0	21.400156	2
ACGCAGA	19300	0.0	21.036083	7
ACATGGG	20965	0.0	20.91703	3
CGCAGAG	19770	0.0	20.535984	8
CATGGGG	15110	0.0	18.966625	4
AGAGTAC	21135	0.0	16.46265	10-11
GCAGAGT	24550	0.0	16.353037	9
GAGTACT	13455	0.0	15.913518	12-13
CAGAGTA	23440	0.0	15.46739	10-11
ATGGGGG	9080	0.0	15.100928	5
GTGGTAT	4520	0.0	14.575613	1
GAGTACA	14960	0.0	14.340075	1
>>END_MODULE