FastQCFastQC Report
Fri 27 May 2016
SRR2139724_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139724_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8587293
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69300.037.869291
GTATCAA108550.031.1210061
TCAACGC124250.026.0204544
ATCAACG128100.025.3329733
CAACGCA128200.025.2816225
GTACATG221600.024.8678461
AACGCAG134100.024.2594916
TACATGG223850.024.0236032
TATCAAC140050.023.5455932
ACATGGG227450.023.182553
CATGGGG172100.020.2615244
ACGCAGA168100.019.2808077
CGCAGAG171850.018.9304478
GAGTACA153650.016.2666651
ATGGGGG103550.015.8445985
AGTACAT158750.015.0076662
GCAGAGT218000.014.9783999
AGAGTAC183600.014.83165410-11
TATACTG38700.014.27074055
CAGAGTA207650.014.07482210-11
Produced by FastQC (version 0.11.5)