##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139724_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8587293
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37419498787336	33.0	31.0	34.0	31.0	34.0
2	32.49053432787259	34.0	31.0	34.0	31.0	34.0
3	32.568893829522295	34.0	31.0	34.0	31.0	34.0
4	36.1151862408794	37.0	35.0	37.0	35.0	37.0
5	36.02463209302396	37.0	35.0	37.0	35.0	37.0
6	36.04760138031857	37.0	35.0	37.0	35.0	37.0
7	35.95788428320776	37.0	35.0	37.0	35.0	37.0
8	35.93487924541529	37.0	35.0	37.0	35.0	37.0
9	37.625747252364626	39.0	37.0	39.0	35.0	39.0
10-11	37.51714556612893	39.0	37.0	39.0	35.0	39.0
12-13	37.47224666725591	39.0	37.0	39.0	35.0	39.0
14-15	38.71353178469629	40.0	38.0	41.0	34.5	41.0
16-17	38.755919414884296	40.0	38.0	41.0	35.0	41.0
18-19	38.71664312607011	40.0	38.0	41.0	34.0	41.0
20-21	38.6734012334862	40.0	38.0	41.0	34.0	41.0
22-23	38.5741658637519	40.0	38.0	41.0	34.0	41.0
24-25	38.495115558027926	40.0	38.0	41.0	34.0	41.0
26-27	38.35168091119739	40.0	38.0	41.0	34.0	41.0
28-29	38.21491343379593	40.0	38.0	41.0	34.0	41.0
30-31	38.072712154899634	40.0	37.5	41.0	33.0	41.0
32-33	38.326198904932156	40.0	38.0	41.0	34.0	41.0
34-35	38.319281718310464	40.0	38.0	41.0	34.0	41.0
36-37	38.22001906961529	40.0	38.0	41.0	33.5	41.0
38-39	38.10650896189175	40.0	38.0	41.0	33.0	41.0
40-41	38.065302347480824	40.0	37.5	41.0	33.0	41.0
42-43	37.957374958769805	40.0	37.0	41.0	33.0	41.0
44-45	37.98232985060588	40.0	37.0	41.0	33.0	41.0
46-47	38.09180837004359	40.0	37.0	41.0	33.0	41.0
48-49	38.01634664509275	40.0	37.0	41.0	33.0	41.0
50-51	37.906853611194876	40.0	37.0	41.0	33.0	41.0
52-53	37.7493748858671	40.0	37.0	41.0	33.0	41.0
54-55	37.619436018031806	39.5	36.0	41.0	32.5	41.0
56-57	37.45761056343965	39.0	36.0	41.0	32.0	41.0
58-59	37.282628319621395	39.0	35.5	41.0	32.0	41.0
60-61	37.02335167721333	39.0	35.0	40.0	31.5	41.0
62-63	36.7522031299781	38.0	35.0	40.0	31.0	41.0
64-65	36.48640597059922	38.0	35.0	40.0	31.0	41.0
66-67	36.17957520521872	37.0	35.0	40.0	31.0	41.0
68-69	35.8398308806421	37.0	34.0	39.0	30.0	41.0
70-71	35.523295324878106	36.0	34.0	39.0	30.0	40.5
72-73	35.19346810690186	36.0	34.0	39.0	30.0	40.0
74-75	34.79684466708955	35.0	34.0	37.5	29.5	40.0
76-77	33.810339140906265	35.0	32.5	36.5	28.5	39.0
78-79	34.11678129170663	35.0	33.0	36.5	29.0	39.0
80-81	34.01215549831436	35.0	33.0	36.0	29.0	38.5
82-83	33.78500004874658	35.0	33.0	36.0	29.0	37.0
84-85	33.559840976580716	35.0	33.0	35.5	29.0	37.0
86-87	33.365329680990186	35.0	33.0	35.0	29.0	36.5
88-89	33.1486775505627	35.0	33.0	35.0	29.0	36.0
90-91	33.03477089536255	34.5	33.0	35.0	29.0	36.0
92-93	32.95473611374992	34.0	33.0	35.0	29.0	35.5
94-95	32.90744359649655	34.0	32.5	35.0	29.0	35.0
96-97	32.866183704343925	34.0	32.0	35.0	29.0	35.0
98-99	32.95894776231705	34.0	32.0	35.0	29.0	35.0
100-101	32.08409373625775	33.5	30.5	35.0	26.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	7.0
20	41.0
21	238.0
22	1087.0
23	3623.0
24	9506.0
25	20719.0
26	37549.0
27	60371.0
28	88474.0
29	121644.0
30	160222.0
31	205847.0
32	265442.0
33	351364.0
34	484486.0
35	692545.0
36	1106570.0
37	1827833.0
38	2012910.0
39	1094193.0
40	42621.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.00647316913491	20.257990498286247	12.528499959183877	26.20703637339497
2	15.501873004604954	22.150806449359003	38.23727721679248	24.11004332924356
3	18.24748726894578	25.862678127041537	30.271145544509487	25.618689059503197
4	12.852175883599173	17.356936580596468	37.32901625692753	32.46187127887683
5	13.186087862612816	37.791513577095834	35.7250299949006	13.297368565390746
6	31.840918901917053	37.10418405427647	17.824790652886772	13.2301063909197
7	27.38782757267046	31.721277007783478	23.077237494982413	17.813657924563657
8	25.495275402853963	36.106046457247935	20.955160141851454	17.443517998046648
9	26.36352340603727	15.083402883772571	20.20102260398009	38.35205110621007
10-11	25.009650887654583	26.012958914992186	28.849062213202693	20.128327984150534
12-13	25.35849190192998	23.585174047281257	28.75465527960907	22.301678771179695
14-15	22.439515558515485	24.967315070608414	25.79227837975911	26.800890991116987
16-17	22.60821121537565	27.87187322102668	26.66368668165044	22.856228881947235
18-19	22.6269717082939	27.451044782828383	27.81536240724224	22.106621101635476
20-21	23.229604374867385	26.84223207861867	27.510688998756937	22.417474547757006
22-23	22.961030254543573	26.755249322815487	27.42941726550583	22.85430315713511
24-25	22.79882744397162	26.828143208691973	27.56555172799049	22.807477619345917
26-27	22.814388572386274	26.94861422281595	27.481425244278622	22.755571960519152
28-29	22.7411475702403	27.042302232455818	27.47080684049005	22.745743356813836
30-31	22.96902710823448	26.936734908072673	27.328950988534594	22.765286995158252
32-33	22.74582291377886	27.102756391402792	27.390721917395037	22.760698777423304
34-35	22.86682852653514	27.089397432354968	27.399167998642593	22.6446060424673
36-37	22.861883407805593	27.006470287808064	27.36985456616309	22.76179173822325
38-39	22.707475513798574	27.138911961887864	27.44839685566225	22.705215668651313
40-41	22.952470170761835	26.908997355322168	27.378368923957456	22.76016354995854
42-43	22.784060993552888	27.05847763492818	27.250551315234073	22.90691005628486
44-45	22.830242035273105	27.114287740829635	27.14715669029626	22.908313533601003
46-47	22.93976797022127	27.05102888631159	26.97263569359726	23.036567449869878
48-49	22.79693742848254	27.164295682186417	27.003527111725333	23.035239777605707
50-51	22.822205820749637	27.223748213338013	26.987164180010186	22.966881785902167
52-53	22.988725597638503	27.114475846809118	26.876970346817647	23.019828208734737
54-55	22.898415832325465	27.01392585653945	26.92522115577311	23.162437155361975
56-57	22.932804041871844	27.17074144453865	26.84434623772415	23.05210827586536
58-59	22.932829987839423	27.21365820004792	26.845294574620933	23.008217237491728
60-61	23.029934533720393	27.154551778193543	26.859251826013963	22.956261862072104
62-63	23.016402214127137	27.247781014735907	26.80287744633909	22.93293932479787
64-65	22.940057062719532	27.206612114474936	26.817169102434523	23.03616172037101
66-67	23.01894551622493	27.229243583430247	26.647571188988778	23.104239711356048
68-69	22.977812804165147	27.173711141620654	26.76684939288535	23.081626661328844
70-71	22.95573644347804	26.96801877862358	26.826613660814402	23.249631117083975
72-73	22.99366479073604	27.018091291202445	26.77177229795788	23.216471620103636
74-75	22.778633878869982	27.20571383387294	26.792908888398802	23.22274339885827
76-77	23.00203287628926	27.092862931346723	26.725912597304692	23.17919159505933
78-79	22.928156822461144	27.081730929606707	26.690059433450163	23.30005281448199
80-81	22.989833628262208	27.117505204830316	26.663877864099018	23.22878330280846
82-83	22.966415538066116	27.0992367359531	26.789172351139023	23.145175374841763
84-85	22.996355770781584	27.07261117458124	26.736817153164367	23.19421590147281
86-87	22.856326030144416	27.097907669616315	26.815479392999386	23.230286907239883
88-89	22.943011354752375	27.07173348870159	26.77456549444579	23.210689662100247
90-91	22.944294624074434	27.097024788439615	26.806666139146884	23.152014448339063
92-93	22.997212344869354	27.05798437408797	26.80123744824425	23.143565832798423
94-95	22.80140396976197	27.08835282597474	26.879218820457368	23.231024383805917
96-97	22.741007041507324	27.106099863385207	26.95406655477594	23.198826540331527
98-99	22.89746626683477	27.727313998833065	27.014367305005948	22.360852429326215
100-101	25.825927982253717	35.64716264845858	28.1862012913192	10.340708077968499
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1879.0
1	1768.5
2	2239.0
3	3002.5
4	3777.5
5	5462.0
6	6967.0
7	8072.5
8	8517.5
9	8144.0
10	7604.0
11	7166.0
12	7850.5
13	10179.0
14	15141.5
15	21287.5
16	25752.0
17	27836.0
18	28363.0
19	28317.5
20	27884.0
21	27786.0
22	29244.5
23	33303.5
24	39958.5
25	49575.5
26	62308.5
27	76129.5
28	90600.0
29	106086.5
30	121863.5
31	138923.5
32	158744.5
33	179463.5
34	199916.5
35	220455.0
36	243870.0
37	265714.0
38	283602.0
39	298866.5
40	313687.5
41	330463.5
42	342989.0
43	354550.0
44	368112.0
45	380305.5
46	389904.5
47	397254.0
48	404053.0
49	406506.0
50	404396.5
51	400940.0
52	392003.0
53	378111.0
54	361966.5
55	341508.0
56	318073.0
57	291825.0
58	263244.5
59	231056.5
60	196770.0
61	163471.5
62	132092.5
63	104222.0
64	79118.5
65	59368.0
66	44662.0
67	34242.5
68	26354.0
69	19206.0
70	13853.5
71	10062.0
72	7091.0
73	4957.0
74	3475.0
75	2397.5
76	1661.0
77	1153.0
78	822.0
79	579.5
80	418.0
81	296.5
82	224.0
83	168.5
84	115.0
85	76.5
86	58.0
87	51.5
88	38.5
89	26.5
90	19.0
91	16.5
92	14.0
93	8.5
94	3.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	2.445473794826845E-4
3	2.67837606100083E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	2.329022661739852E-5
16-17	1.7467669963048892E-5
18-19	6.987067985219557E-5
20-21	0.0
22-23	1.1646966017763719E-4
24-25	0.00205036989954469
26-27	0.0031059427757140446
28-29	0.003277598222678624
30-31	0.004943655764483772
32-33	0.0024626365268100203
34-35	0.0062917543025172076
36-37	0.0207749131619311
38-39	0.00871492967549234
40-41	0.0033490378783451397
42-43	0.008035064950006351
44-45	0.0048192254637466955
46-47	0.003857004873646028
48-49	0.0025542434359606264
50-51	7.746687951982929E-5
52-53	4.5758339228532707E-5
54-55	0.0029867571426707663
56-57	0.0027142874515064665
58-59	0.00456230948607118
60-61	0.001834686570454318
62-63	0.0014402058834026177
64-65	2.921972154460579E-4
66-67	5.066262735951235E-5
68-69	1.029898315932343E-4
70-71	1.7950474640466952E-4
72-73	9.198013776344138E-4
74-75	0.0026253763940362568
76-77	0.0030208187768445845
78-79	0.0011332081377751263
80-81	0.0065571564659413
82-83	0.0042853532334768815
84-85	0.007348189807530455
86-87	0.003837984948245458
88-89	0.052374400747891424
90-91	0.0
92-93	1.2748545413733553E-4
94-95	0.0014819419777773945
96-97	0.0229639936872548
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	301.0
20-21	760.0
22-23	1561.0
24-25	3301.0
26-27	5408.0
28-29	13351.0
30-31	27016.0
32-33	36666.0
34-35	52825.0
36-37	81737.0
38-39	87554.0
40-41	84253.0
42-43	94089.0
44-45	110448.0
46-47	111283.0
48-49	106730.0
50-51	97498.0
52-53	96492.0
54-55	106363.0
56-57	110412.0
58-59	110012.0
60-61	107075.0
62-63	100722.0
64-65	105304.0
66-67	111353.0
68-69	112111.0
70-71	109406.0
72-73	102559.0
74-75	102571.0
76-77	104954.0
78-79	111452.0
80-81	111534.0
82-83	104483.0
84-85	102958.0
86-87	107567.0
88-89	114419.0
90-91	119900.0
92-93	122912.0
94-95	149419.0
96-97	197245.0
98-99	671433.0
100-101	4379856.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.11742578182371
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.44744677712215	79.66666157543213
2	6.075445322999481	10.585543140358704
3	1.3728378680130333	3.5879430323130754
4	0.49990196549080457	1.7420068950733183
5	0.22243188790847726	0.968884674318885
6	0.12718886624069797	0.6648219968998985
7	0.07163775554788727	0.43686277964836495
8	0.040607107162427415	0.2830069315549866
9	0.029199340809745584	0.22893942652840682
>10	0.10908276297907957	1.5241949590049495
>50	0.0033468777834288663	0.1949136784929972
>100	8.519902219745025E-4	0.1024497339496387
>500	2.1477720864319234E-5	0.013771176424730023
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.329022661739852E-5
2	0.0	3.4935339926097785E-5	0.0	0.0	2.329022661739852E-5
3	0.0	3.4935339926097785E-5	0.0	0.0	2.329022661739852E-5
4	0.0	8.151579316089483E-5	0.0	0.0	3.4935339926097785E-5
5	0.0	8.151579316089483E-5	0.0	0.0	3.4935339926097785E-5
6	3.4935339926097785E-5	8.151579316089483E-5	0.0	0.0	5.8225566543496306E-5
7	3.4935339926097785E-5	8.151579316089483E-5	0.0	0.0	5.8225566543496306E-5
8	3.4935339926097785E-5	9.316090646959408E-5	0.0	0.0	5.8225566543496306E-5
9	3.4935339926097785E-5	2.2125715286528595E-4	0.0	1.164511330869926E-5	6.987067985219557E-5
10-11	3.4935339926097785E-5	2.2125715286528595E-4	0.0	2.329022661739852E-5	6.987067985219557E-5
12-13	3.4935339926097785E-5	2.2125715286528595E-4	0.0	2.329022661739852E-5	6.987067985219557E-5
14-15	3.4935339926097785E-5	2.853052760631319E-4	0.0	2.329022661739852E-5	1.5720902966744E-4
16-17	6.987067985219557E-5	3.085955026805304E-4	0.0	2.329022661739852E-5	1.746766996304889E-4
18-19	6.987067985219557E-5	3.2606317264357925E-4	0.0	2.329022661739852E-5	1.8632181293918817E-4
20-21	6.987067985219557E-5	3.3770828595227855E-4	0.0	3.4935339926097785E-5	1.921443695935378E-4
22-23	6.987067985219557E-5	3.493533992609778E-4	0.0	8.151579316089483E-5	2.329022661739852E-4
24-25	6.987067985219557E-5	3.7264362587837634E-4	0.0	1.5720902966744E-4	2.853052760631319E-4
26-27	6.987067985219557E-5	3.7846618253272596E-4	0.0	3.260631726435793E-4	2.911278327174815E-4
28-29	6.987067985219557E-5	4.017564091501245E-4	0.0	0.0015022196168222048	2.911278327174815E-4
30-31	6.987067985219557E-5	4.1922407911317337E-4	0.0	0.006247603290117153	2.911278327174815E-4
32-33	6.987067985219557E-5	4.2504663576752304E-4	0.0	0.014585504419145823	2.911278327174815E-4
34-35	6.987067985219557E-5	5.065624289284179E-4	0.0	0.02645187488071037	3.0277294602618077E-4
36-37	6.987067985219557E-5	5.589654388175646E-4	0.0	0.04298211322240897	3.0277294602618077E-4
38-39	6.987067985219557E-5	5.880782220893127E-4	0.0	0.0644673472769591	3.0277294602618077E-4
40-41	6.987067985219557E-5	6.987067985219556E-4	0.0	0.0855857602622852	3.0277294602618077E-4
42-43	8.151579316089483E-5	7.39464695102403E-4	0.0	0.10175500009141414	3.0277294602618077E-4
44-45	8.733834981524446E-5	7.918677049915496E-4	0.0	0.12286759052008589	3.085955026805304E-4
46-47	9.316090646959408E-5	8.268030449176475E-4	0.0	0.13922897471880835	3.2024061598922963E-4
48-49	9.316090646959408E-5	8.268030449176475E-4	0.0	0.15209682492492105	3.260631726435793E-4
50-51	1.2227368974134225E-4	8.908511681154934E-4	0.0	0.16359637431726157	3.3188572929792893E-4
52-53	1.3974135970439114E-4	8.966737247698431E-4	0.0	0.17401292817189307	3.7264362587837634E-4
54-55	1.3974135970439114E-4	8.966737247698431E-4	0.0	0.1839578549375222	4.075789658044741E-4
56-57	1.3974135970439114E-4	9.083188380785423E-4	0.0	0.19428707044233845	4.075789658044741E-4
58-59	1.3974135970439114E-4	9.083188380785423E-4	0.0	0.20476184986351345	4.075789658044741E-4
60-61	1.4556391635874076E-4	9.257865080415913E-4	0.0	0.21507359769836665	4.075789658044741E-4
62-63	1.5138647301309038E-4	9.548992913133394E-4	0.0	0.22441297857194345	4.3086919242187266E-4
64-65	1.5138647301309038E-4	0.0010829955377090312	0.0	0.2342705669877574	4.3086919242187266E-4
66-67	1.5138647301309038E-4	0.0010946406510177304	0.0	0.24381373734423642	4.3086919242187266E-4
68-69	1.5138647301309038E-4	0.0011528662175612269	0.0	0.2532113437843567	4.3086919242187266E-4
70-71	1.5138647301309038E-4	0.0011878015574873246	0.0	0.2629117231705032	4.425143057305719E-4
72-73	1.7467669963048892E-4	0.0011878015574873246	0.0	0.2700560001853902	5.123849855827675E-4
74-75	1.8632181293918817E-4	0.0011994466707960238	0.0	0.2762803132488899	5.182075422371171E-4
76-77	1.8632181293918817E-4	0.0012285594540677718	0.0	0.2822018533663635	5.240300988914667E-4
78-79	1.8632181293918817E-4	0.0012460271240308208	0.0	0.2874130415720064	5.240300988914667E-4
80-81	1.8632181293918817E-4	0.0012460271240308208	0.0	0.29069696352505964	5.589654388175646E-4
82-83	1.8632181293918817E-4	0.0012460271240308208	0.0	0.2924961335312537	6.055458920523615E-4
84-85	1.9796692624788744E-4	0.0012460271240308208	0.0	0.29394595013818675	6.055458920523615E-4
86-87	1.9796692624788744E-4	0.00125767223733952	0.0	0.2941380945077803	6.055458920523615E-4
88-89	2.0378948290223706E-4	0.001292607577265618	0.0	0.294167207291052	6.055458920523615E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6930	0.0	37.86929	1
GTATCAA	10855	0.0	31.121006	1
TCAACGC	12425	0.0	26.020454	4
ATCAACG	12810	0.0	25.332973	3
CAACGCA	12820	0.0	25.281622	5
GTACATG	22160	0.0	24.867846	1
AACGCAG	13410	0.0	24.259491	6
TACATGG	22385	0.0	24.023603	2
TATCAAC	14005	0.0	23.545593	2
ACATGGG	22745	0.0	23.18255	3
CATGGGG	17210	0.0	20.261524	4
ACGCAGA	16810	0.0	19.280807	7
CGCAGAG	17185	0.0	18.930447	8
GAGTACA	15365	0.0	16.266665	1
ATGGGGG	10355	0.0	15.844598	5
AGTACAT	15875	0.0	15.007666	2
GCAGAGT	21800	0.0	14.978399	9
AGAGTAC	18360	0.0	14.831654	10-11
TATACTG	3870	0.0	14.2707405	5
CAGAGTA	20765	0.0	14.074822	10-11
>>END_MODULE