FastQCFastQC Report
Fri 27 May 2016
SRR2139723_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139723_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9647342
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78850.040.807591
GTATCAA135200.032.4645161
TCAACGC154650.027.1361264
GTACATG282800.026.6714571
TACATGG279200.026.5698932
ATCAACG158500.026.4771183
CAACGCA159400.026.2519785
AACGCAG165950.025.5301136
ACATGGG287850.025.2836253
TATCAAC171600.024.7364122
CATGGGG193400.021.9065254
ACGCAGA203050.020.6875827
CGCAGAG206600.020.3321088
GAGTACA187000.018.966141
AGTACAT191150.017.92472
ATGGGGG116350.017.8964565
GCGTGCG22600.016.3323129
GCAGAGT263850.015.9813689
AGAGTAC225750.015.33738510-11
GAGTACT138800.014.56831612-13
Produced by FastQC (version 0.11.5)