FastQCFastQC Report
Fri 27 May 2016
SRR2139722_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139722_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8262202
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64250.042.5476761
GTATCAA105450.030.8989491
GTACATG213050.026.5088461
TCAACGC120700.026.0781694
TACATGG215200.025.8250162
CAACGCA124600.025.2948115
ACATGGG215450.025.281433
ATCAACG127500.024.783773
AACGCAG130550.024.1733596
TATCAAC137300.023.3132952
CATGGGG165900.022.4070194
ACGCAGA160250.019.693187
CGCAGAG166850.018.8896228
ATGGGGG96650.017.8950845
GAGTACA146850.017.7503241
AGTACAT149550.016.7995342
AGAGTAC177800.015.44422910-11
GAGTACT111050.015.07641212-13
TATACCG5500.014.9035745
GCAGAGT216750.014.4652359
Produced by FastQC (version 0.11.5)