##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139722_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8262202
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.446404118417824	34.0	31.0	34.0	31.0	34.0
2	32.55429182196223	34.0	31.0	34.0	31.0	34.0
3	32.63552137795711	34.0	31.0	34.0	31.0	34.0
4	36.158891055919476	37.0	35.0	37.0	35.0	37.0
5	36.075353156458775	37.0	35.0	37.0	35.0	37.0
6	36.09909041197492	37.0	35.0	37.0	35.0	37.0
7	36.0139242540911	37.0	35.0	37.0	35.0	37.0
8	35.990953017125456	37.0	35.0	37.0	35.0	37.0
9	37.697560650296374	39.0	37.0	39.0	35.0	39.0
10-11	37.59429943736549	39.0	37.0	39.0	35.0	39.0
12-13	37.55143550109281	39.0	37.0	39.0	35.0	39.0
14-15	38.81642750927658	40.0	38.0	41.0	35.0	41.0
16-17	38.860805751299715	40.0	38.0	41.0	35.0	41.0
18-19	38.824693526011586	40.0	38.0	41.0	35.0	41.0
20-21	38.782880291209736	40.0	38.0	41.0	34.5	41.0
22-23	38.68522581143628	40.0	38.0	41.0	34.0	41.0
24-25	38.608923337525184	40.0	38.0	41.0	34.0	41.0
26-27	38.47244859708951	40.0	38.0	41.0	34.0	41.0
28-29	38.340234275266155	40.0	38.0	41.0	34.0	41.0
30-31	38.19979672732742	40.0	38.0	41.0	34.0	41.0
32-33	38.44904219168229	40.0	38.0	41.0	34.0	41.0
34-35	38.44421173854663	40.0	38.0	41.0	34.0	41.0
36-37	38.34750077545813	40.0	38.0	41.0	34.0	41.0
38-39	38.237603630706914	40.0	38.0	41.0	34.0	41.0
40-41	38.19650163606724	40.0	38.0	41.0	33.5	41.0
42-43	38.09314103993178	40.0	37.5	41.0	33.0	41.0
44-45	38.11594421244415	40.0	37.5	41.0	33.0	41.0
46-47	38.217792331445	40.0	38.0	41.0	33.0	41.0
48-49	38.14233314362108	40.0	37.0	41.0	33.0	41.0
50-51	38.03251334546348	40.0	37.0	41.0	33.0	41.0
52-53	37.87967483670237	40.0	37.0	41.0	33.0	41.0
54-55	37.752220864321856	40.0	36.5	41.0	33.0	41.0
56-57	37.59324365318851	39.0	36.0	41.0	33.0	41.0
58-59	37.422909507050036	39.0	36.0	41.0	32.0	41.0
60-61	37.16913297136027	39.0	35.0	40.5	32.0	41.0
62-63	36.90569674132045	38.5	35.0	40.0	31.5	41.0
64-65	36.640810187954884	38.0	35.0	40.0	31.0	41.0
66-67	36.33860950814183	37.0	35.0	40.0	31.0	41.0
68-69	36.006194859299406	37.0	34.5	39.5	31.0	41.0
70-71	35.690876867529866	36.0	34.0	39.0	30.5	41.0
72-73	35.363168061428226	36.0	34.0	39.0	30.0	40.0
74-75	34.97061034472866	35.0	34.0	38.0	30.0	40.0
76-77	33.9864788108795	35.0	32.5	37.0	29.0	39.0
78-79	34.279508283491595	35.0	33.5	37.0	29.0	39.0
80-81	34.17021041548736	35.0	34.0	36.0	30.0	39.0
82-83	33.93856675365568	35.0	33.5	36.0	29.0	37.5
84-85	33.712189924040885	35.0	33.0	35.5	29.0	37.0
86-87	33.52050902439165	35.0	33.0	35.0	29.0	37.0
88-89	33.30405872983113	35.0	33.0	35.0	29.0	36.0
90-91	33.18910049593673	35.0	33.0	35.0	29.0	36.0
92-93	33.10974636408276	35.0	33.0	35.0	29.0	36.0
94-95	33.064240533230674	34.0	33.0	35.0	29.0	35.0
96-97	33.01882018805355	34.0	33.0	35.0	29.0	35.0
98-99	33.09661317286718	34.0	33.0	35.0	29.0	35.0
100-101	32.22287864146068	33.5	31.0	35.0	26.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	4.0
20	45.0
21	215.0
22	926.0
23	3255.0
24	8401.0
25	18276.0
26	33753.0
27	54434.0
28	79667.0
29	109534.0
30	144852.0
31	186875.0
32	241921.0
33	319233.0
34	443080.0
35	638093.0
36	1035351.0
37	1759210.0
38	2038078.0
39	1099034.0
40	47962.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.699016799637675	19.95650796240518	12.1563355628439	26.18813967511324
2	15.56572396284182	22.15602106559615	38.139563931702384	24.138691039859637
3	18.281007195794526	25.90296007582496	30.252099111288572	25.563933617091937
4	12.720955019013092	17.36821491413548	37.53092698532426	32.379903081527175
5	13.118597197212075	37.8673506167	35.77294527536364	13.241106910724284
6	31.617588144177546	37.242577705071845	17.97699935198873	13.16283479876188
7	27.395360219951048	31.81472687305394	23.12499742804642	17.66491547894859
8	25.668568742328013	35.868113609422764	21.112930911154194	17.350386737095025
9	26.493954033077383	15.03099294836897	20.16462439432006	38.31042862423359
10-11	24.995939339173745	26.051184659973213	28.91007143132061	20.042804569532432
12-13	25.404075087972917	23.566707761441805	28.8535489691489	22.17566818143638
14-15	22.41347205263295	24.990981518785464	25.867860505200763	26.72768592338082
16-17	22.560439853712115	27.88597566883354	26.746945109000308	22.806639368454036
18-19	22.58029845955519	27.440013947873272	27.914185963857168	22.06550162871437
20-21	23.2272898734466	26.85659873816374	27.616156360165643	22.299955028224012
22-23	22.902841451960608	26.781307835603414	27.561102299315714	22.75474841312026
24-25	22.73735135876001	26.845365890040956	27.678968823418593	22.738313927780442
26-27	22.72064955333216	27.038680932421773	27.61124908931358	22.62942042493249
28-29	22.68437411389992	27.070485797522036	27.597478053503522	22.64766203507452
30-31	22.915732054675885	27.0236950732668	27.45692206290462	22.603650809152693
32-33	22.668136849975458	27.17655429651315	27.49339750242478	22.661911351086612
34-35	22.80860888371486	27.116941647461047	27.513081110981414	22.561368357842685
36-37	22.841312926320708	27.045394767117482	27.489366423857795	22.623925882704015
38-39	22.622804244940667	27.19403967407432	27.57764192862313	22.60551415236188
40-41	22.870637173315934	27.03408508164295	27.47612060724191	22.619157137799206
42-43	22.686191626599257	27.14666786631053	27.349378696000983	22.817761811089234
44-45	22.761791325686623	27.22673198682021	27.267489185943216	22.74398750154996
46-47	22.874211243257577	27.114216556227788	27.11423588080421	22.89733631971043
48-49	22.71054511426736	27.23412531684426	27.13637597713479	22.918953591753585
50-51	22.727593308831306	27.32367964796462	27.116984613098815	22.83174243010526
52-53	22.87312304168353	27.273308294101955	26.9664956510914	22.887073013123114
54-55	22.848704707139262	27.106374689268222	27.030121036558285	23.01479956703423
56-57	22.82294445997572	27.303501703911635	27.015342590459955	22.858211245652686
58-59	22.8049544589111	27.355811627282815	26.975429534049095	22.863804379756985
60-61	22.878527901002162	27.290238224230244	27.018032982124502	22.81320089264309
62-63	22.856804205374807	27.382661082421873	26.9818464528447	22.778688259358624
64-65	22.81291347452473	27.342553308005712	26.95097121404537	22.893562003424183
66-67	22.86713395389711	27.381415077175113	26.811752074943847	22.939698893983934
68-69	22.854954232452148	27.34210069426349	26.86490435971741	22.938040713566952
70-71	22.822715764742547	27.107882182166964	27.004448539228616	23.064953513861877
72-73	22.87606084973701	27.16710381971213	26.94431173711748	23.012523593433375
74-75	22.65727824343375	27.356357561381405	26.934661716588693	23.051702478596155
76-77	22.869640946835226	27.27603143566355	26.88477734101727	22.969550276483954
78-79	22.82644350348114	27.258196471720563	26.812779307084817	23.10258071771348
80-81	22.851536349527297	27.27463466896065	26.79729441322749	23.076534568284565
82-83	22.81296538610102	27.231130905279464	26.95291831167237	23.002985396947146
84-85	22.838115415041234	27.24869880524348	26.88283645178704	23.03034932792824
86-87	22.710410400217576	27.279108992016	26.954844947029965	23.055635660736463
88-89	22.80939075178528	27.24277069935263	26.8918872037676	23.055951345094492
90-91	22.802250936378687	27.28357068215706	26.952343052300904	22.96183532916335
92-93	22.825515110469652	27.255243190254635	26.94273012034225	22.976511578933465
94-95	22.65903082361047	27.28314012722297	27.050519551192153	23.00730949797441
96-97	22.614898188102607	27.314240387272058	27.090093113796442	22.980768310828893
98-99	22.79808973559566	27.945570937619145	27.114426144552617	22.141913182232578
100-101	25.67438519236227	35.81941536204966	28.293530912998527	10.21266853258954
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1895.0
1	1733.0
2	2145.5
3	2933.0
4	3793.5
5	5692.0
6	7313.0
7	8388.0
8	8916.5
9	8640.5
10	7946.0
11	7232.5
12	7881.5
13	10142.0
14	15014.0
15	21191.5
16	25833.5
17	28126.5
18	28357.0
19	27593.5
20	26804.0
21	26540.0
22	27785.5
23	31818.5
24	38463.5
25	47651.0
26	59934.0
27	73543.5
28	87246.5
29	101908.5
30	116840.0
31	132980.5
32	152016.5
33	171974.5
34	191092.5
35	210814.5
36	231925.0
37	250083.0
38	266167.5
39	281380.0
40	295737.5
41	309299.0
42	320613.5
43	333170.5
44	345927.5
45	356070.5
46	364251.0
47	371115.0
48	375556.0
49	376109.5
50	374279.5
51	371372.0
52	363583.0
53	350957.5
54	336635.0
55	318333.0
56	297785.0
57	275113.0
58	247385.5
59	217717.5
60	186479.0
61	154754.5
62	125893.0
63	99103.0
64	74943.0
65	56294.0
66	42333.0
67	32277.0
68	24624.5
69	17751.5
70	12698.5
71	9100.5
72	6247.5
73	4162.0
74	2801.5
75	1934.0
76	1364.0
77	958.5
78	670.5
79	490.0
80	360.0
81	246.5
82	185.0
83	134.5
84	91.5
85	63.0
86	47.0
87	38.5
88	27.0
89	18.0
90	13.0
91	9.5
92	8.0
93	4.5
94	1.5
95	1.0
96	1.0
97	1.0
98	1.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.2103310957538923E-4
3	1.8154966436308384E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.8154966436308384E-5
16-17	6.051655478769462E-6
18-19	1.270847650541587E-4
20-21	0.0
22-23	1.271027408374509E-4
24-25	0.0019190473195297936
26-27	0.003185408455357893
28-29	0.0030784354445724366
30-31	0.00494051624510326
32-33	0.0023550452889814
34-35	0.006376526174815396
36-37	0.021026493381660894
38-39	0.009121054222964511
40-41	0.0035223201747820237
42-43	0.007849994295208801
44-45	0.0045637323310519975
46-47	0.0038067966328078624
48-49	0.0023993392428363425
50-51	1.1875598356971382E-4
52-53	4.0028596429289085E-5
54-55	0.0027741331188363707
56-57	0.0030430952203642017
58-59	0.004811484444865969
60-61	0.002242665552129847
62-63	0.001425453147992114
64-65	3.2510722939501084E-4
66-67	6.597474794530811E-5
68-69	7.445019646662345E-5
70-71	1.2859219458994484E-4
72-73	9.911333517679014E-4
74-75	0.002878413699189855
76-77	0.0033278231448990456
78-79	0.0011761007940935895
80-81	0.006312866827778679
82-83	0.004322242719962312
84-85	0.007035972320875305
86-87	0.0037481774055547817
88-89	0.05679588155057861
90-91	0.0
92-93	1.9260886413508303E-4
94-95	0.0014169675767789981
96-97	0.0248786597716226
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	230.0
20-21	692.0
22-23	1297.0
24-25	2702.0
26-27	4321.0
28-29	10405.0
30-31	19975.0
32-33	27514.0
34-35	39931.0
36-37	62935.0
38-39	69347.0
40-41	68696.0
42-43	76728.0
44-45	91133.0
46-47	94328.0
48-49	91666.0
50-51	85094.0
52-53	84707.0
54-55	93999.0
56-57	99646.0
58-59	99788.0
60-61	98442.0
62-63	94046.0
64-65	97564.0
66-67	104689.0
68-69	105978.0
70-71	103990.0
72-73	98273.0
74-75	98565.0
76-77	101647.0
78-79	108160.0
80-81	107801.0
82-83	101638.0
84-85	100650.0
86-87	106450.0
88-89	111829.0
90-91	116487.0
92-93	120338.0
94-95	144402.0
96-97	190318.0
98-99	654205.0
100-101	4371596.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.60810984673026
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.69608012440096	80.33320260053098
2	5.904928130578284	10.346391846014997
3	1.3602460672990975	3.5750576064756645
4	0.46390183301413407	1.6256625097920707
5	0.21804270829803413	0.9551154769926369
6	0.11468309628001971	0.6028301577877865
7	0.06649119243224552	0.4077617383310972
8	0.04464857844578306	0.3129262051982812
9	0.029194669548118425	0.23019208350394949
>10	0.0989182487091272	1.368221701280759
>50	0.0022843995069829473	0.1382753435833161
>100	5.38092448675912E-4	0.07237184001453935
>500	4.2859038511095996E-5	0.03199089049386173
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.630993287261677E-5
2	0.0	2.4206621915077845E-5	0.0	0.0	3.630993287261677E-5
3	0.0	2.4206621915077845E-5	0.0	0.0	3.630993287261677E-5
4	0.0	1.089297986178503E-4	0.0	0.0	3.630993287261677E-5
5	0.0	1.3313642053292815E-4	0.0	0.0	3.630993287261677E-5
6	3.630993287261677E-5	1.3313642053292815E-4	0.0	0.0	4.841324383015569E-5
7	3.630993287261677E-5	1.3313642053292815E-4	0.0	1.2103310957538923E-5	4.841324383015569E-5
8	3.630993287261677E-5	1.57343042448006E-4	0.0	1.2103310957538923E-5	7.261986574523354E-5
9	3.630993287261677E-5	2.9047946298093414E-4	0.0	6.051655478769461E-5	7.261986574523354E-5
10-11	7.261986574523354E-5	2.9047946298093414E-4	0.0	7.8671521224003E-5	7.261986574523354E-5
12-13	7.261986574523354E-5	3.0258277393847307E-4	0.0	8.472317670277246E-5	7.261986574523354E-5
14-15	7.261986574523354E-5	4.236158835138623E-4	0.0	8.472317670277246E-5	1.1498145409661977E-4
16-17	1.2103310957538923E-4	4.357191944714012E-4	0.0	8.472317670277246E-5	1.2103310957538923E-4
18-19	1.2103310957538923E-4	4.4782250542894014E-4	0.0	1.1498145409661977E-4	1.2103310957538923E-4
20-21	1.2103310957538923E-4	4.5992581638647906E-4	0.0	1.4523973149046707E-4	1.2103310957538923E-4
22-23	1.4523973149046707E-4	5.143907156954042E-4	0.0	2.1180794175693115E-4	1.5129138696923653E-4
24-25	1.4523973149046707E-4	5.870105814406377E-4	0.0	2.4206621915077845E-4	1.9365297532062276E-4
26-27	1.4523973149046707E-4	6.051655478769461E-4	0.0	3.267893958535509E-4	1.9970463079939222E-4
28-29	1.5129138696923653E-4	6.112172033557156E-4	0.0	8.653867334640329E-4	2.0575628627816169E-4
30-31	1.57343042448006E-4	6.414754807495628E-4	0.0	0.002886639663373033	2.0575628627816169E-4
32-33	1.57343042448006E-4	6.414754807495628E-4	0.0	0.005888260780842686	2.178595972357006E-4
34-35	1.57343042448006E-4	7.927668677187995E-4	0.0	0.010487518944707477	2.2996290819323953E-4
36-37	1.6339469792677546E-4	9.501099101668054E-4	0.0	0.016914377063160645	2.2996290819323953E-4
38-39	1.6944635340554492E-4	0.0010106264649545	0.0	0.02551377949849205	2.4206621915077845E-4
40-41	1.6944635340554492E-4	0.0011861244738388143	0.0	0.03486963886866963	2.541695301083174E-4
42-43	1.6944635340554492E-4	0.0012284860621902005	0.0	0.04399553533065398	2.6022118558708686E-4
44-45	1.6944635340554492E-4	0.001428190692989593	0.0	0.05482194698217255	2.662728410658563E-4
46-47	1.8154966436308384E-4	0.001585533735437599	0.0	0.06603566458433235	2.7232449654462573E-4
48-49	1.9365297532062276E-4	0.0015915853909163682	0.0	0.07722517556457709	3.14686084896012E-4
50-51	2.0575628627816169E-4	0.001863909887460994	0.0	0.08836022164551291	3.14686084896012E-4
52-53	2.178595972357006E-4	0.001876013198418533	0.0	0.09775239094856311	3.3889270681108983E-4
54-55	2.2996290819323953E-4	0.0019002198203336108	0.0	0.10765289931182995	3.630993287261677E-4
56-57	2.481178746295479E-4	0.0019607363751213054	0.0	0.11934469769681255	3.691509842049371E-4
58-59	2.541695301083174E-4	0.0019728396860788444	0.0	0.12978380339768986	3.752026396837066E-4
60-61	2.783761520233952E-4	0.0020030979634726915	0.0	0.14036814883005766	3.812542951624761E-4
62-63	2.783761520233952E-4	0.0020273045853877692	0.0	0.1498692479317257	3.9940926159878445E-4
64-65	2.783761520233952E-4	0.002178595972357006	0.0	0.16012075231276118	3.9940926159878445E-4
66-67	2.783761520233952E-4	0.002190699283314545	0.0	0.17060221960198987	3.9940926159878445E-4
68-69	2.9047946298093414E-4	0.0022149059052296227	0.0	0.1812591849001029	3.9940926159878445E-4
70-71	2.9047946298093414E-4	0.0022330608716659315	0.0	0.19143806941539315	4.1151257255632337E-4
72-73	2.9047946298093414E-4	0.002239112527144701	0.0	0.1994686162357202	4.357191944714012E-4
74-75	2.9047946298093414E-4	0.0022512158381022395	0.0	0.20625252202742078	4.357191944714012E-4
76-77	2.965311184597036E-4	0.002281474115496087	0.0	0.2125886053136924	4.4177084995017065E-4
78-79	3.0258277393847307E-4	0.0022875257709748563	0.0	0.21879155217943108	4.4782250542894014E-4
80-81	3.0258277393847307E-4	0.0022875257709748563	0.0	0.22217442759206324	4.538741609077096E-4
82-83	3.0258277393847307E-4	0.0022875257709748563	0.0	0.2240988540343119	4.5992581638647906E-4
84-85	3.0258277393847307E-4	0.002299629081932395	0.0	0.22567228445879198	4.5992581638647906E-4
86-87	3.0863442941724255E-4	0.002323835703847473	0.0	0.22596881557725168	4.5992581638647906E-4
88-89	3.14686084896012E-4	0.002396455569592707	0.0	0.226065642064912	4.5992581638647906E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6425	0.0	42.547676	1
GTATCAA	10545	0.0	30.898949	1
GTACATG	21305	0.0	26.508846	1
TCAACGC	12070	0.0	26.078169	4
TACATGG	21520	0.0	25.825016	2
CAACGCA	12460	0.0	25.294811	5
ACATGGG	21545	0.0	25.28143	3
ATCAACG	12750	0.0	24.78377	3
AACGCAG	13055	0.0	24.173359	6
TATCAAC	13730	0.0	23.313295	2
CATGGGG	16590	0.0	22.407019	4
ACGCAGA	16025	0.0	19.69318	7
CGCAGAG	16685	0.0	18.889622	8
ATGGGGG	9665	0.0	17.895084	5
GAGTACA	14685	0.0	17.750324	1
AGTACAT	14955	0.0	16.799534	2
AGAGTAC	17780	0.0	15.444229	10-11
GAGTACT	11105	0.0	15.076412	12-13
TATACCG	550	0.0	14.903574	5
GCAGAGT	21675	0.0	14.465235	9
>>END_MODULE