FastQCFastQC Report
Fri 27 May 2016
SRR2139721_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139721_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11445036
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103300.042.0874981
GTATCAA186450.034.048191
ATCAACG216100.028.452453
TCAACGC215650.028.4173494
CAACGCA222200.027.6530155
GTACATG375200.027.0303121
AACGCAG227400.027.0206686
TACATGG373800.026.5756192
TATCAAC234450.026.4343092
ACATGGG376400.025.9155083
CATGGGG256950.022.9300444
ACGCAGA279850.021.9709647
CGCAGAG283000.021.740818
ATGGGGG147200.020.4283715
GAGTACA248800.020.012891
AGTACAT258500.018.394982
TATACCG8400.017.462585
GCAGAGT350300.017.4359959
AGAGTAC310700.015.99284610-11
GAGTACT185100.015.497169512-13
Produced by FastQC (version 0.11.5)