##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139721_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11445036
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.44070084183222	34.0	31.0	34.0	31.0	34.0
2	32.53621176901497	34.0	31.0	34.0	31.0	34.0
3	32.623252473823584	34.0	31.0	34.0	31.0	34.0
4	36.15083604804738	37.0	35.0	37.0	35.0	37.0
5	36.074989017072554	37.0	35.0	37.0	35.0	37.0
6	36.10361880906272	37.0	35.0	37.0	35.0	37.0
7	36.01782012743341	37.0	35.0	37.0	35.0	37.0
8	35.99598026602975	37.0	35.0	37.0	35.0	37.0
9	37.6955655709602	39.0	37.0	39.0	35.0	39.0
10-11	37.595045048351096	39.0	37.0	39.0	35.0	39.0
12-13	37.5568802055319	39.0	37.0	39.0	35.0	39.0
14-15	38.81471923723088	40.0	38.0	41.0	35.0	41.0
16-17	38.86820666182265	40.0	38.0	41.0	35.0	41.0
18-19	38.848670812394126	40.0	38.0	41.0	35.0	41.0
20-21	38.81916530921908	40.0	38.0	41.0	35.0	41.0
22-23	38.72668577020673	40.0	38.0	41.0	34.0	41.0
24-25	38.65864054793126	40.0	38.0	41.0	34.0	41.0
26-27	38.52922388555233	40.0	38.0	41.0	34.0	41.0
28-29	38.40101676635791	40.0	38.0	41.0	34.0	41.0
30-31	38.265723395869	40.0	38.0	41.0	34.0	41.0
32-33	38.4837820528527	40.0	38.0	41.0	34.0	41.0
34-35	38.46544959051772	40.0	38.0	41.0	34.0	41.0
36-37	38.37296462869067	40.0	38.0	41.0	34.0	41.0
38-39	38.269314053871916	40.0	38.0	41.0	34.0	41.0
40-41	38.23730854838131	40.0	38.0	41.0	34.0	41.0
42-43	38.14034543605278	40.0	38.0	41.0	33.0	41.0
44-45	38.16685303529542	40.0	37.5	41.0	33.5	41.0
46-47	38.269861703087635	40.0	38.0	41.0	33.5	41.0
48-49	38.202948483226116	40.0	38.0	41.0	33.0	41.0
50-51	38.10466519474596	40.0	37.0	41.0	33.0	41.0
52-53	37.970135504842986	40.0	37.0	41.0	33.0	41.0
54-55	37.86833015284253	40.0	37.0	41.0	33.0	41.0
56-57	37.72745137583125	40.0	36.5	41.0	33.0	41.0
58-59	37.58222350438446	39.0	36.0	41.0	32.5	41.0
60-61	37.35453240822072	39.0	36.0	41.0	32.0	41.0
62-63	37.11898859466053	39.0	35.0	40.0	32.0	41.0
64-65	36.88778442994814	38.0	35.0	40.0	31.5	41.0
66-67	36.607291999081255	38.0	35.0	40.0	31.0	41.0
68-69	36.29466443769137	37.0	35.0	40.0	31.0	41.0
70-71	35.99706550233742	37.0	34.5	39.0	31.0	41.0
72-73	35.67553973377176	36.0	34.0	39.0	31.0	40.5
74-75	35.28239622983592	36.0	34.0	39.0	30.0	40.0
76-77	34.27357386174026	35.0	33.0	37.0	29.0	39.0
78-79	34.562449637077606	35.0	34.0	37.0	30.0	39.0
80-81	34.43445694974635	35.0	34.0	37.0	30.0	39.0
82-83	34.18392878114656	35.0	34.0	36.0	30.0	38.5
84-85	33.935085545730814	35.0	34.0	36.0	30.0	37.0
86-87	33.71951771868217	35.0	33.0	35.5	29.0	37.0
88-89	33.48435345551614	35.0	33.0	35.0	29.0	36.5
90-91	33.348923283689565	35.0	33.0	35.0	29.0	36.0
92-93	33.253231227729174	35.0	33.0	35.0	29.0	36.0
94-95	33.189995573259864	35.0	33.0	35.0	29.0	36.0
96-97	33.13032674215196	34.0	33.0	35.0	29.0	35.0
98-99	33.19766682491826	34.0	33.0	35.0	29.0	35.0
100-101	32.35368396265331	34.0	31.5	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	3.0
19	19.0
20	62.0
21	299.0
22	1286.0
23	4248.0
24	11123.0
25	24001.0
26	43860.0
27	71190.0
28	105576.0
29	144376.0
30	192397.0
31	248746.0
32	322926.0
33	425963.0
34	591980.0
35	841542.0
36	1338530.0
37	2314959.0
38	2948296.0
39	1731122.0
40	82530.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.10506074423881	20.891188109849544	12.536753925457289	25.466997220454353
2	14.780099381293734	22.81455212231775	39.45847671094418	22.94687178544433
3	17.614441449153215	27.453977505113368	30.900023512448797	24.03155753328462
4	12.056248665360249	18.051642651014816	39.00319754345902	30.88891114016592
5	12.237698509642083	38.95310595790175	36.34600188238814	12.46319365006803
6	29.646258867163024	38.62050761570344	18.935265909167956	12.797967607965585
7	26.114937515268625	32.761845397428196	24.22321782124582	16.899999266057357
8	24.83344744393989	36.646210636646316	21.883941649462702	16.636400269951096
9	26.315976638256096	15.41080342604427	21.083245172841742	37.189974762857894
10-11	24.174419372730675	26.761112852768658	29.68877948483517	19.375688289665494
12-13	24.70634430507689	24.397581624033336	29.643554638010748	21.252519432879023
14-15	21.773806953033382	25.737277221600095	26.839135071120584	25.64978075424594
16-17	21.62964988748396	28.738811196296037	27.679736234970008	21.95180268124999
18-19	21.61346712772293	28.242841605225117	29.024023890207655	21.119667376844294
20-21	22.278874146689613	27.64018773658649	28.681635776552426	21.399302340171467
22-23	22.03041700561552	27.615302250620378	28.561165073486904	21.7931156702772
24-25	21.800049201924075	27.69701649199721	28.736602713343203	21.76633159273551
26-27	21.797099424179965	27.87560688801502	28.646771326029985	21.680522361775033
28-29	21.703378886859085	27.858232117453674	28.6946351682925	21.743753827394745
30-31	21.93900427194239	27.865230132243312	28.525522255537915	21.670243340276382
32-33	21.84619645772184	27.932948109712395	28.54069036132481	21.680165071240953
34-35	21.90927846102622	27.92734988297979	28.608265666951727	21.555105989042264
36-37	21.82825522827371	27.931839130312408	28.560891938630572	21.67901370278331
38-39	21.639065021500276	28.00989769334054	28.65103300574201	21.700004279417172
40-41	21.844407205610704	27.800756309869968	28.633525286089746	21.721311198429582
42-43	21.741419207385615	27.916263806416858	28.42256406563404	21.919752920563482
44-45	21.734214659608902	28.047629617325526	28.339021462873976	21.879134260191595
46-47	21.870104289018272	27.974809165203368	28.125004358975765	22.030082186802595
48-49	21.76633230401214	28.095120558065865	28.090266853477015	22.048280284444978
50-51	21.796542571825757	28.173524965913693	28.02691309662475	22.003019365635797
52-53	21.860199251884417	28.109826746800508	28.011244301033873	22.018729700281202
54-55	21.86395240029817	28.046082016033303	27.963922022182125	22.126043561486405
56-57	21.89887442368084	28.156102597729166	27.893355210404863	22.05166776818513
58-59	21.83782199931005	28.20037197875101	27.904916605718473	22.056889416220468
60-61	21.9405282148213	28.26567637967625	27.828442826746304	21.965352578756146
62-63	21.90962782292017	28.28457498656659	27.833682391092555	21.972114799420677
64-65	21.83468915666942	28.24284195725667	27.833363493779046	22.089105392294865
66-67	21.952081126775425	28.22533423884966	27.719259733489547	22.10332490088537
68-69	21.93570101929761	28.167640798631798	27.805101093987428	22.09155708808317
70-71	21.947828360710105	27.960993929403028	27.886733357318978	22.204444352567887
72-73	21.96849530884683	28.064819649731703	27.824453257866388	22.14223178355508
74-75	21.834353902888864	28.208817484622145	27.786250330645444	22.170578281843543
76-77	21.917162578825998	28.099546035017354	27.830602893099233	22.152688493057422
78-79	21.950343397379687	28.03449328092385	27.774181267301127	22.24098205439534
80-81	21.94943455205804	28.12011389801277	27.719219241697928	22.211232308231256
82-83	21.970442339515145	27.982475071984968	27.86586113163252	22.181221456867366
84-85	22.00009658777277	28.017005166202757	27.80778138196716	22.17511686405731
86-87	21.85531739126113	28.072477363882083	27.918050766722896	22.154154478133893
88-89	21.995300920574522	27.893999860697182	27.849753771287777	22.260945447440516
90-91	21.990590737047057	27.903477360119837	27.92916441968129	22.176767483151817
92-93	22.030344238645046	27.90567930398908	27.892256772487485	22.17171968487839
94-95	21.89780012395986	27.83280535487779	28.01478971253497	22.254604808627374
96-97	21.8434072356525	27.851531983262866	28.11460467140588	22.190456109678752
98-99	21.977832918237333	28.49798741635134	28.123930786946776	21.40024887846455
100-101	24.604539678188956	36.231186123205916	29.48562450239452	9.678649696210602
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2382.0
1	2199.0
2	3202.0
3	4709.5
4	6273.0
5	9671.5
6	12542.0
7	14705.5
8	15851.5
9	15159.5
10	13643.0
11	12399.5
12	13573.5
13	17597.0
14	26227.5
15	37429.0
16	45775.5
17	49503.0
18	49884.5
19	48950.0
20	47745.0
21	47140.0
22	49281.0
23	56286.5
24	68250.0
25	84840.5
26	106074.5
27	129083.0
28	153581.5
29	181671.5
30	209492.5
31	238057.0
32	270588.0
33	304389.5
34	337631.0
35	368944.0
36	399233.5
37	424232.0
38	442170.5
39	455555.5
40	466395.5
41	475981.5
42	481663.0
43	488753.5
44	495005.5
45	496846.0
46	499932.0
47	501259.0
48	496372.0
49	486044.5
50	475598.5
51	461695.5
52	439483.5
53	414979.5
54	387584.0
55	357288.0
56	327211.5
57	294957.5
58	261624.5
59	228606.0
60	195123.0
61	162144.5
62	131180.5
63	103628.5
64	79511.0
65	59769.5
66	44320.5
67	33492.5
68	25648.0
69	18387.0
70	12715.0
71	8872.5
72	6074.0
73	4063.5
74	2802.0
75	2013.5
76	1427.0
77	958.0
78	665.0
79	503.5
80	384.5
81	283.5
82	198.5
83	131.0
84	97.5
85	82.0
86	66.5
87	53.0
88	39.5
89	25.5
90	15.5
91	14.0
92	12.0
93	7.5
94	3.5
95	2.0
96	2.0
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	2.184353111689644E-4
3	3.058094356365502E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	2.1843531116896445E-5
16-17	8.737412446758576E-6
18-19	1.0484894936110294E-4
20-21	0.0
22-23	9.175532524968918E-5
24-25	0.002058414391594327
26-27	0.0031084501534223447
28-29	0.0031628734131879397
30-31	0.0048497639934858075
32-33	0.0023058773035220144
34-35	0.0062849949931596686
36-37	0.022839772525792126
38-39	0.010572348159618605
40-41	0.004176466194186846
42-43	0.007782683828514926
44-45	0.004588818230479114
46-47	0.0037241701946708846
48-49	0.002438562236886549
50-51	9.05599152740498E-5
52-53	4.8220699583980734E-5
54-55	0.002918165988306938
56-57	0.0027296143425805447
58-59	0.004408539285426437
60-61	0.0018832974522752066
62-63	0.0014769430851719512
64-65	2.723598678363266E-4
66-67	6.38136539203679E-5
68-69	2.0002299723865548E-4
70-71	3.023853641965063E-4
72-73	0.0011796229801961427
74-75	0.003510854163549221
76-77	0.003799498939633757
78-79	0.001586526604964101
80-81	0.0062693871216010204
82-83	0.004284490668660074
84-85	0.007066443838551957
86-87	0.003577053272896116
88-89	0.05427665492297655
90-91	6.598268139364924E-6
92-93	1.9580113248674326E-4
94-95	0.0015489556063099747
96-97	0.025514615339765477
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	340.0
20-21	901.0
22-23	1750.0
24-25	4257.0
26-27	5690.0
28-29	13193.0
30-31	26868.0
32-33	36331.0
34-35	51855.0
36-37	85037.0
38-39	97238.0
40-41	96800.0
42-43	109020.0
44-45	127793.0
46-47	133672.0
48-49	132403.0
50-51	123577.0
52-53	119901.0
54-55	132080.0
56-57	140995.0
58-59	145599.0
60-61	144136.0
62-63	135111.0
64-65	139771.0
66-67	153413.0
68-69	154224.0
70-71	153396.0
72-73	143406.0
74-75	141483.0
76-77	146367.0
78-79	158311.0
80-81	159990.0
82-83	148846.0
84-85	146407.0
86-87	152022.0
88-89	162740.0
90-91	172475.0
92-93	171393.0
94-95	198251.0
96-97	251989.0
98-99	871417.0
100-101	5954588.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.12617494862025
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.52885259188324	75.31719916094002
2	6.970147110022021	11.727436303906646
3	1.8660894350089205	4.709608988379971
4	0.741058781134884	2.493697626758581
5	0.33742467406782684	1.419312358130558
6	0.18972999222585557	0.9576755111395611
7	0.11497783965997217	0.6770852098114458
8	0.0693257286862029	0.46656867039168687
9	0.046069336706285643	0.3488073371567891
>10	0.13387678832576091	1.6762436688550006
>50	0.001963536337174216	0.11015305354247235
>100	4.4914935577771987E-4	0.07165951332021786
>500	3.503658610780096E-5	0.024552597667039627
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.737412446758576E-6
2	0.0	8.737412446758577E-5	0.0	0.0	8.737412446758576E-6
3	0.0	8.737412446758577E-5	0.0	0.0	8.737412446758576E-6
4	0.0	1.8348566138193012E-4	0.0	8.737412446758577E-5	8.737412446758576E-6
5	0.0	1.8348566138193012E-4	0.0	8.737412446758577E-5	8.737412446758576E-6
6	0.0	1.8348566138193012E-4	0.0	8.737412446758577E-5	1.7474824893517153E-5
7	0.0	1.8348566138193012E-4	0.0	9.611153691434435E-5	1.7474824893517153E-5
8	8.737412446758576E-6	2.0969789872220585E-4	0.0	9.611153691434435E-5	1.7474824893517153E-5
9	8.737412446758576E-6	3.2328426053006737E-4	0.0	2.4464754850924016E-4	3.4949649787034306E-5
10-11	1.7474824893517153E-5	3.2328426053006737E-4	0.0	2.4464754850924016E-4	3.4949649787034306E-5
12-13	2.621223734027573E-5	3.2328426053006737E-4	0.0	2.927033169664123E-4	3.931835601041359E-5
14-15	2.621223734027573E-5	4.062896787742738E-4	0.0	3.2328426053006737E-4	4.8055768457172177E-5
16-17	3.058094356365502E-5	4.106583849976531E-4	0.0	3.320216729768259E-4	7.86367120208272E-5
18-19	3.4949649787034306E-5	4.54345447231446E-4	0.0	3.7134002898723953E-4	7.86367120208272E-5
20-21	3.931835601041359E-5	4.980325094652389E-4	0.0	4.6308285967820457E-4	7.86367120208272E-5
22-23	4.3687062233792884E-5	5.28613453028894E-4	0.0	6.20356283719859E-4	9.174283069096506E-5
24-25	4.3687062233792884E-5	5.985127526029625E-4	0.0	9.174283069096506E-4	1.0921765558448222E-4
26-27	4.3687062233792884E-5	6.290936961666176E-4	0.0	0.001529047178182751	1.1358636180786151E-4
28-29	4.3687062233792884E-5	6.290936961666176E-4	0.0	0.005063330512896595	1.1358636180786151E-4
30-31	4.3687062233792884E-5	6.378311086133762E-4	0.0	0.01455216043007641	1.1358636180786151E-4
32-33	5.242447468055146E-5	6.421998148367555E-4	0.0	0.030873646880621435	1.3106118670137867E-4
34-35	6.989929957406861E-5	8.082106513251685E-4	0.0	0.05354723217995994	1.3106118670137867E-4
36-37	6.989929957406861E-5	9.742214878135814E-4	0.0	0.08495822992605703	1.3106118670137867E-4
38-39	6.989929957406861E-5	0.0010048024313772364	0.0	0.13047141136122245	1.3979859914813725E-4
40-41	1.3106118670137867E-4	0.0010921765558448223	0.0	0.17535986780644464	1.6601083648841298E-4
42-43	1.3106118670137867E-4	0.001122757499408477	0.0	0.21541216646238598	1.6601083648841298E-4
44-45	1.4853601159489584E-4	0.0012669248047799935	0.0	0.25150204857372227	1.6601083648841298E-4
46-47	1.7037954271179224E-4	0.0013805111665878551	0.0	0.28137963043541325	1.8348566138193012E-4
48-49	1.7474824893517154E-4	0.0013848798728112344	0.0	0.30444639929485584	2.0096048627544726E-4
50-51	1.922230738286887E-4	0.001529047178182751	0.0	0.321541146746939	2.0096048627544726E-4
52-53	1.922230738286887E-4	0.0015377845906295096	0.0	0.3342890315067598	2.4901625473261945E-4
54-55	1.96591780052068E-4	0.001542153296852889	0.0	0.34707623462259096	2.970720231897916E-4
56-57	2.0096048627544726E-4	0.001572734240416544	0.0	0.35773587780763644	2.970720231897916E-4
58-59	2.0096048627544726E-4	0.0015771029466399233	0.0	0.36857900665406385	2.970720231897916E-4
60-61	2.0096048627544726E-4	0.0015989464777568197	0.0	0.37918185665820536	2.970720231897916E-4
62-63	2.0096048627544726E-4	0.0016382648337672332	0.0	0.38883669741187354	3.058094356365502E-4
64-65	2.0969789872220585E-4	0.0017736947266919912	0.0	0.3987667666576147	3.058094356365502E-4
66-67	2.1406660494558514E-4	0.0017824321391387498	0.0	0.4089939079265456	3.101781418599295E-4
68-69	2.3154142983910228E-4	0.0018042756702556463	0.0	0.4196360762866976	3.145468480833088E-4
70-71	2.3591013606248158E-4	0.0018261192013725427	0.0	0.4302039766410521	3.145468480833088E-4
72-73	2.3591013606248158E-4	0.0018261192013725427	0.0	0.43845646269701555	3.407590854235845E-4
74-75	2.4464754850924016E-4	0.0018654375573829564	0.0	0.4453764933548483	3.407590854235845E-4
76-77	2.4464754850924016E-4	0.0019353368569570248	0.0	0.4517810166783224	3.407590854235845E-4
78-79	2.4464754850924016E-4	0.00196591780052068	0.0	0.457294323932227	3.407590854235845E-4
80-81	2.4464754850924016E-4	0.00196591780052068	0.0	0.4606407528993356	3.4949649787034307E-4
82-83	2.4464754850924016E-4	0.001970286506744059	0.0	0.46265035776209007	3.6697132276386024E-4
84-85	2.4464754850924016E-4	0.0020008674503077142	0.0	0.46412698046559225	3.6697132276386024E-4
86-87	2.4464754850924016E-4	0.0020314483938713695	0.0	0.4643104661269742	3.6697132276386024E-4
88-89	2.5775366717937804E-4	0.0021188225183389553	0.0	0.4643672593078781	3.6697132276386024E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10330	0.0	42.087498	1
GTATCAA	18645	0.0	34.04819	1
ATCAACG	21610	0.0	28.45245	3
TCAACGC	21565	0.0	28.417349	4
CAACGCA	22220	0.0	27.653015	5
GTACATG	37520	0.0	27.030312	1
AACGCAG	22740	0.0	27.020668	6
TACATGG	37380	0.0	26.575619	2
TATCAAC	23445	0.0	26.434309	2
ACATGGG	37640	0.0	25.915508	3
CATGGGG	25695	0.0	22.930044	4
ACGCAGA	27985	0.0	21.970964	7
CGCAGAG	28300	0.0	21.74081	8
ATGGGGG	14720	0.0	20.428371	5
GAGTACA	24880	0.0	20.01289	1
AGTACAT	25850	0.0	18.39498	2
TATACCG	840	0.0	17.46258	5
GCAGAGT	35030	0.0	17.435995	9
AGAGTAC	31070	0.0	15.992846	10-11
GAGTACT	18510	0.0	15.4971695	12-13
>>END_MODULE